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Symmetry breaking in the female germline cyst.
In mammals and flies, only one cell in a multicellular female germline cyst becomes an oocyte, but how symmetry is broken to select the oocyte is unknown. Here, we show that the microtubule (MT) minus end-stabilizing protein Patronin/CAMSAP marks the future Drosophila oocyte and is required for oocyte specification. The spectraplakin Shot recruits Patronin to the fusome, a branched structure extending into all cyst cells. Patronin stabilizes more MTs in the cell with the most fusome material. Our data suggest that this weak asymmetry is amplified by Dynein-dependent transport of Patronin-stabilized MTs. This forms a polarized MT network, along which Dynein transports oocyte determinants into the presumptive oocyte. Thus, Patronin amplifies a weak fusome anisotropy to break symmetry and select one cell to become the oocyte
Nonequilibrium functional RG with frequency dependent vertex function: A study of the single impurity Anderson model
We investigate nonequilibrium properties of the single impurity Anderson
model by means of the functional renormalization group (fRG) within Keldysh
formalism. We present how the level broadening Gamma/2 can be used as flow
parameter for the fRG. This choice preserves important aspects of the Fermi
liquid behaviour that the model exhibits in case of particle-hole symmetry. An
approximation scheme for the Keldysh fRG is developed which accounts for the
frequency dependence of the two-particle vertex in a way similar but not
equivalent to a recently published approximation to the equilibrium Matsubara
fRG. Our method turns out to be a flexible tool for the study of weak to
intermediate on-site interactions U <= 3 Gamma. In equilibrium we find
excellent agreement with NRG results for the linear conductance at finite gate
voltage, magnetic field, and temperature. In nonequilibrium, our results for
the current agree well with TD-DMRG. For the nonlinear conductance as function
of the bias voltage, we propose reliable results at finite magnetic field and
finite temperature. Furthermore, we demonstrate the exponentially small scale
of the Kondo temperature to appear in the second order derivative of the
self-energy. We show that the approximation is, however, not able to reproduce
the scaling of the effective mass at large interactions.Comment: [v2] - minor changes throughout the text; added new Fig. 3; corrected
pert.-theory data in Figs. 10, 11; published versio
Time- and angle-resolved photoemission spectroscopy with optimized high-harmonic pulses using frequency-doubled Ti:Sapphire lasers
Time- and angle-resolved photoemission spectroscopy (trARPES) using femtosecond extreme ultraviolet high harmonics has recently emerged as a powerful tool for investigating ultrafast quasiparticle dynamics in correlated-electron materials. However, the full potential of this approach has not yet been achieved because, to date, high harmonics generated by 800 nm wavelength Ti:Sapphire lasers required a trade-off between photon flux, energy and time resolution. Photoemission spectroscopy requires a quasi-monochromatic output, but dispersive optical elements that select a single harmonic can significantly reduce the photon flux and time resolution. Here we show that 400 nm driven high harmonic extreme-ultraviolet trARPES is superior to using 800 nm laser drivers since it eliminates the need for any spectral selection, thereby increasing photon flux and energy resolution to < 150 meV while preserving excellent time resolution of about 30 fs. © 2014 The Authors
Charge transport through single molecules, quantum dots, and quantum wires
We review recent progresses in the theoretical description of correlation and
quantum fluctuation phenomena in charge transport through single molecules,
quantum dots, and quantum wires. A variety of physical phenomena is addressed,
relating to co-tunneling, pair-tunneling, adiabatic quantum pumping, charge and
spin fluctuations, and inhomogeneous Luttinger liquids. We review theoretical
many-body methods to treat correlation effects, quantum fluctuations,
nonequilibrium physics, and the time evolution into the stationary state of
complex nanoelectronic systems.Comment: 48 pages, 14 figures, Topical Review for Nanotechnolog
DNA-PAINT MINFLUX nanoscopy
MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations, has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on–off switching process for single fluorophore separation, initial MINFLUX implementations have been limited to two color channels. Here we show that MINFLUX can be effectively combined with sequentially multiplexed DNA-based labeling (DNA-PAINT), expanding MINFLUX nanoscopy to multiple molecular targets. Our method is exemplified with three-color recordings of mitochondria in human cells
Monitoring mitochondrial translation in living cells
Mitochondria possess a small genome that codes for core subunits of the oxidative phosphorylation system and whose expression is essential for energy production. Information on the regulation and spatial organization of mitochondrial gene expression in the cellular context has been difficult to obtain. Here we devise an imaging approach to analyze mitochondrial translation within the context of single cells, by following the incorporation of clickable non-canonical amino acids. We apply this method to multiple cell types, including specialized cells such as cardiomyocytes and neurons, and monitor with spatial resolution mitochondrial translation in axons and dendrites. We also show that translation imaging allows to monitor mitochondrial protein expression in patient fibroblasts. Approaching mitochondrial translation with click chemistry opens new avenues to understand how mitochondrial biogenesis is integrated into the cellular context and can be used to assess mitochondrial gene expression in mitochondrial diseases
Self-amplified photo-induced gap quenching in a correlated electron material.
Capturing the dynamic electronic band structure of a correlated material presents a powerful capability for uncovering the complex couplings between the electronic and structural degrees of freedom. When combined with ultrafast laser excitation, new phases of matter can result, since far-from-equilibrium excited states are instantaneously populated. Here, we elucidate a general relation between ultrafast non-equilibrium electron dynamics and the size of the characteristic energy gap in a correlated electron material. We show that carrier multiplication via impact ionization can be one of the most important processes in a gapped material, and that the speed of carrier multiplication critically depends on the size of the energy gap. In the case of the charge-density wave material 1T-TiSe2, our data indicate that carrier multiplication and gap dynamics mutually amplify each other, which explains-on a microscopic level-the extremely fast response of this material to ultrafast optical excitation
Systematic behaviour of the in-plane penetration depth in d-wave cuprates
We report the temperature T and oxygen concentration dependences of the
penetration depth of grain-aligned YBa_2Cu_3O_{7-\delta} with \delta= 0.0, 0.3
and 0.43. The values of the in-plane \lambda_{ab}(0) and out-of-plane
\lambda_{c}(0) penetration depths, the low temperature linear term in
\lambda_{ab}(T), and the ratio \lambda_{c}(0) /\lambda_{ab}(T) were found to
increase with increasing . The systematic changes of the linear term in
\lambda_{ab}(T) with T_c found here and in recent work on HgBa_2Ca_{n-1}
Cu_nO_{2n+2+\delta} (n = 1 and 3) are discussed.Comment: 4 pages, 4 figure
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