Into the blue – The blue economy model in Operation Phakisa ‘Unlocking the Ocean Economy’ Programme

Significance: Economic and social benefits of ocean resource uses have motivated numerous nations, including South Africa, to turn to their Exclusive Economic Zones (EEZs) to advance economic development initiatives. Such initiatives result in increasing and spatially competitive pressures on ocean systems, compromising ecosystem services and market and non-market ocean benefits. It is critical to prioritise sustainable development in any ocean or blue economy advancement programmes (where the blue economy model most often parallels a terrestrial green economy, to incorporate sustainability and inclusivity pillars over and above the often GDP-centred ocean economy model). We explore multiple definitions of ocean and blue economies, discuss the importance of adopting blue economy models, and examine how the South African Operation Phakisa – Unlocking the Ocean Economy initiative presents numerous features aligned with ocean sustainable development, sustainability, and inclusivity, which strongly align it to a blue economy model

Baseline adjustment methodology in a shared water savings contract during severe water restrictions – a case study in the Western Cape, South Africa

A novel method for baseline adjustment in a shared water savings contract under serious drought conditions was presented in a companion paper. The newly developed baseline adjustment method was subsequently applied to a case study, as discussed in this manuscript. The case study involved application of the method to 24 relatively complex sites, spread over the Western Cape Province in South Africa. The sites included, for example, military bases, naval dockyards, an airforce base, prison facilities, large multi-storey blocks of flats and administrative office buildings. Baseline adjustment became essential mid-contract during the serious water restrictions in Cape Town at the time. The restrictions were linked to the ‘Day Zero’ scenario in 2018 when water supply would potentially run out, and resulted in water savings at baseline sites that were ascribed to external factors. The study incorporated a comprehensive review of the approved baseline reports with site visits to 12 of the properties. The baseline adjustment method provided a robust means to obtain adjustments for sites with relatively limited data. The minimum data requirement was a record of monthly water consumption per site. The adjustments varied between 0% and 64% of the original baseline value for the different sites in the study sample. The relatively higher adjustments were linked to sites where outdoor irrigation and pool water use was prevalent during the baseline-setting period, but was banned during the drought. Zero adjustments were found for sites with exceptionally high leakage flows that had subsequently been repaired; leaks dwarfed actual use in these cases. The results for all 24 sites were accepted by the contracting parties as being reasonable and fair.Keywords: baselines, shared water savings contrac

Concurrent babesiosis and ehrlichiosis in the dog : blood smear examination supplemented by the indirect fluorescent antibody test, using Cowdria ruminantium as antigen

Giemsa-stained, peripheral blood smears of 67 dogs, showing clinical signs typical of babesiosis or reminiscent of concurrent babesiosis and ehrlichiosis, were examined for the presence of Babesia canis and Ehrlichia canis. Since Cowdria ruminantium cross-reacts with Ehrlichia, the sera of these dogs were also subjected to the indirect fluorescent antibody (IFA) test in which C. ruminantium was used as antigen. Fifty-five per cent of these dogs had mixed infections of B. canis and E. canis, as judged by blood smear examination and serology. The serum of 32 % of these dogs with mixed infections reacted positively in the IFA test. Six out of 9 dogs, the blood smears of which were negative for both B. canis and E. canis, were serologically positive for E. canis. Furthermore, sero-conversion from a negative in the initial serum sample to titres of up to 1:160 in a subsequent sample was recorded in 9 out of 13 dogs with suspected mixed infection on blood smear.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201

Potential Antioxidative Effects of Kolaviron on Reproductive Function in Streptozotocin-Induced Diabetic Wistar Rats

The present study investigated the effects of Kolaviron (KV) on the testicular and epididymal tissue antioxidant status in streptozotocin (STZ)-induced diabetic rats. Diabetes was induced by a single intraperitoneal injection of STZ at 50 mg/kg body weight. The antioxidant status was studied by evaluating epididymal and testicular levels of malondialdehyde (MDA), a lipid peroxidation (LPO) marker, and the activities of catalase (CAT) glutathione peroxidase (GPX) and superoxide dismutase (SOD) were also assessed using biochemical techniques. Diabetes induction resulted in testicular and epididymal LPO and adversely affected the activities of antioxidant enzymes evident by a noticeable decrease in enzyme activity in both tissues. The potential antioxidative effects of KV in the testicular and epididymal tissues of STZ-induced diabetes were revealed by its ability to mitigate against LPO and increase the activity of antioxidant defense enzymes in the reproductive tissues studied. KV might potentially be used as an antioxidant as well as antidiabetic treatment; however, further studies are needed

Baseline adjustment methodology in a shared water savings contract under serious drought conditions

CITATION: Jacobs, H. E. et al. 2020. Baseline adjustment methodology in a shared water savings contract under serious drought conditions. Water SA, 46(1):22-29, doi:10.17159/wsa/2020.v46.i1.7874.The original publication is available at: http://www.scielo.org.zaENGLISH ABSTRACT: Baselines are often employed in shared water saving contracts for estimating water savings after some type of intervention by the water service company. An adjustment to the baseline may become necessary under certain conditions. Earlier work has described a number of relatively complex methods for baseline determination and adjustment, but application in regions faced with relatively limited data becomes problematic. If the adjustment were determined before finalising the contractual matters, it would be possible to gather the required data in order to determine the adjustment. However, in cases where no adjustment was fixed prior to the contract, a method is required to determine an adjustment mid-contract based on whatever data are available at the time. This paper presents a methodology for baseline adjustment in an existing shared water savings contract and explains how adjustment could be determined mid-contract, under conditions of limited data. The adjustment compensates for expected reduced water consumption due to external influences induced by serious water restrictions, typically introduced during periods of drought. The fundamental principle underpinning the baseline adjustment methodology presented in this paper involved segregating real water losses from the actual consumption of end-users, preferably by analysing the minimum night flow. In the absence of recorded night flows, an alternative procedure involving the minimum monthly consumption pre- and post-baseline was employed. The baseline adjustment method was subsequently applied in a South African case study, reported on separately. This technique is helpful because adjustments could be determined without adding unnecessary complexity or cost, and provides a means to resolve disputes in cases where unexpected savings occur mid-contract.Publisher's versio

Baseline adjustment methodology in a shared water savings contract under serious drought conditions

CITATION: Jacobs, H. E. et al. 2020. Baseline adjustment methodology in a shared water savings contract under serious drought conditions. Water SA, 46(1):22-29, doi:10.17159/wsa/2020.v46.i1.7874.The original publication is available at: http://www.scielo.org.zaENGLISH ABSTRACT: Baselines are often employed in shared water saving contracts for estimating water savings after some type of intervention by the water service company. An adjustment to the baseline may become necessary under certain conditions. Earlier work has described a number of relatively complex methods for baseline determination and adjustment, but application in regions faced with relatively limited data becomes problematic. If the adjustment were determined before finalising the contractual matters, it would be possible to gather the required data in order to determine the adjustment. However, in cases where no adjustment was fixed prior to the contract, a method is required to determine an adjustment mid-contract based on whatever data are available at the time. This paper presents a methodology for baseline adjustment in an existing shared water savings contract and explains how adjustment could be determined mid-contract, under conditions of limited data. The adjustment compensates for expected reduced water consumption due to external influences induced by serious water restrictions, typically introduced during periods of drought. The fundamental principle underpinning the baseline adjustment methodology presented in this paper involved segregating real water losses from the actual consumption of end-users, preferably by analysing the minimum night flow. In the absence of recorded night flows, an alternative procedure involving the minimum monthly consumption pre- and post-baseline was employed. The baseline adjustment method was subsequently applied in a South African case study, reported on separately. This technique is helpful because adjustments could be determined without adding unnecessary complexity or cost, and provides a means to resolve disputes in cases where unexpected savings occur mid-contract.Publisher's versio

HIV and influenza infection are associated with increased blood pneumococcal load : a prospective, hospital-based observational study in South Africa, 2009–2011

BACKGROUND : Increased pneumococcal loads are associated with severe outcomes. We determined the prevalence of pneumococcal DNA in blood specimens from patients hospitalized with acute lower respiratory tract infection and identified factors associated with invasive pneumococcal pneumonia, bacterial loads, and death. METHODS : A total of 8523 patients were enrolled as part of prospective hospital-based surveillance. Blood was collected for quantitative pneumococcal (lytA) detection, and nasopharyngeal specimens were collected for detection of influenza virus and other respiratory viruses by real-time polymerase chain reaction. RESULTS : Of 6396 cases (75%) with lytA results, 422 (7%) were positive for pneumococcal DNA. The prevalences of human immunodeficiency virus (HIV) and influenza virus were 51% (2965/5855) and 8% (485/6358), respectively. On multivariable analysis, HIV infection (adjusted odds ratio [aOR], 2.4; 95% confidence interval [CI], 1.6–3.6), influenza virus coinfection (aOR, 1.4; 95% CI, 1.2–2.1), oxygen therapy during admission (aOR, 1.6; 95% CI, 1.1– 2.3) and in-hospital death (aOR, 2.1; 95% CI, 1.1–4.0) were significantly associated with increased pneumococcal load. Among lytA-positive patients, after adjustment for length of hospitalization, duration of symptoms, and oxygen therapy during admission, pneumococcal loads ≥10,000 DNA copies/mL (aOR, 3.6; 95% CI, 1.8–7.2) were associated with increased risk of death. CONCLUSIONS : HIV and influenza virus infections were associated with elevated pneumococcal loads, which, in turn, were associated with increased risk of death.http://jid.oxfordjournals.orghb201

Epidemiology and molecular identification and characterization of mycoplasma pneumoniae, South Africa, 2012-2015.

CAPRISA, 2018.Abstract available in pdf

Visualizing variation within global pneumococcal sequence clusters (GPSCS) and country population snapshots to contextualize pneumococcal isolates

Knowledge of pneumococcal lineages, their geographic distribution and antibiotic resistance patterns, can give insights into global pneumococcal disease. We provide interactive bioinformatic outputs to explore such topics, aiming to increase dissemi-nation of genomic insights to the wider community, without the need for specialist training. We prepared 12 country-specific phylogenetic snapshots, and international phylogenetic snapshots of 73 common Global Pneumococcal Sequence Clusters (GPSCs) previously defined using PopPUNK, and present them in Microreact. Gene presence and absence defined using Roary, and recombination profiles derived from Gubbins are presented in Phandango for each GPSC. Temporal phylogenetic signal was assessed for each GPSC using BactDating. We provide examples of how such resources can be used. In our example use of a country-specific phylogenetic snapshot we determined that serotype 14 was observed in nine unrelated genetic backgrounds in South Africa. The international phylogenetic snapshot of GPSC9, in which most serotype 14 isolates from South Africa were observed, highlights that there were three independent sub-clusters represented by South African serotype 14 isolates. We estimated from the GPSC9-dated tree that the sub-clusters were each established in South Africa during the 1980s. We show how recombination plots allowed the identification of a 20 kb recombination spanning the capsular polysaccharide locus within GPSC97. This was consistent with a switch from serotype 6A to 19A estimated to have occured in the 1990s from the GPSC97-dated tree. Plots of gene presence/absence of resistance genes (tet, erm, cat) across the GPSC23 phylogeny were consistent with acquisition of a composite transposon. We estimated from the GPSC23-dated tree that the acquisition occurred between 1953 and 1975. Finally, we demonstrate the assignment of GPSC31 to 17 externally generated pneumococcal serotype 1 assemblies from Utah via Pathogenwatch. Most of the Utah isolates clustered within GPSC31 in a USA-specific clade with the most recent common ancestor estimated between 1958 and 1981. The resources we have provided can be used to explore to data, test hypothesis and generate new hypotheses. The accessible assignment of GPSCs allows others to contextualize their own collections beyond the data presented here.Fil: Gladstone, Rebecca A.. Wellcome Sanger Institute; Reino UnidoFil: Lo, Stephanie W.. Wellcome Sanger Institute; Reino UnidoFil: Goater, Richard. Wellcome Sanger Institute; Reino Unido. University of Oxford; Reino UnidoFil: Yeats, Corin. Wellcome Sanger Institute; Reino Unido. University of Oxford; Reino UnidoFil: Taylor, Ben. Wellcome Sanger Institute; Reino Unido. University of Oxford; Reino UnidoFil: Hadfield, James. Fred Hutchinson Cancer Research Center; Estados UnidosFil: Lees, John A.. Imperial College London; Reino UnidoFil: Croucher, Nicholas J.. Imperial College London; Reino UnidoFil: van Tonder, Andries. Wellcome Sanger Institute; Reino Unido. University of Cambridge; Estados UnidosFil: Bentley, Leon J.. Wellcome Sanger Institute; Reino UnidoFil: Quah, Fu Xiang. Wellcome Sanger Institute; Reino UnidoFil: Blaschke, Anne J.. University of Utah; Estados UnidosFil: Pershing, Nicole L.. University of Utah; Estados UnidosFil: Byington, Carrie L.. University of California; Estados UnidosFil: Balaji, Veeraraghavan. Christian Medical College; IndiaFil: Hryniewicz, Waleria. National Medicines Institute; PoloniaFil: Sigauque, Betuel. Instituto Nacional de Saude Maputo; MozambiqueFil: Ravikumar, K. L.. Kempegowda Institute Of Medical Sciences; IndiaFil: Grassi Almeida, Samanta Cristine. Adolfo Lutz Institute; BrasilFil: Ochoa, Theresa J.. Universidad Peruana Cayetano Heredia; PerúFil: Ho, Pak Leung. The University Of Hong Kong; Hong KongFil: du Plessis, Mignon. National Institute for Communicable Diseases; SudáfricaFil: Ndlangisa, Kedibone M.. National Institute for Communicable Diseases; SudáfricaFil: Cornick, Jennifer. Malawi liverpool wellcome Trust Clinical Research Programme; MalauiFil: Kwambana Adams, Brenda. Colegio Universitario de Londres; Reino Unido. Medical Research Council Unit The Gambia at The London School of Hygiene & Tropical Medicine; GambiaFil: Benisty, Rachel. Ben Gurion University of the Negev; IsraelFil: Nzenze, Susan A.. University of the Witwatersrand; SudáfricaFil: Madhi, Shabir A.. University of the Witwatersrand; SudáfricaFil: Hawkins, Paulina A.. Emory University; Estados UnidosFil: Faccone, Diego Francisco. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Data from a pre-publication independent replication initiative examining ten moral judgement effects

We present the data from a crowdsourced project seeking to replicate findings in independent laboratories before (rather than after) they are published. In this Pre-Publication Independent Replication (PPIR) initiative, 25 research groups attempted to replicate 10 moral judgment effects from a single laboratory's research pipeline of unpublished findings. The 10 effects were investigated using online/lab surveys containing psychological manipulations (vignettes) followed by questionnaires. Results revealed a mix of reliable, unreliable, and culturally moderated findings. Unlike any previous replication project, this dataset includes the data from not only the replications but also from the original studies, creating a unique corpus that researchers can use to better understand reproducibility and irreproducibility in science