Garden footprint area and water use of gated communities in South Africa

Gated community homes in South Africa are popular amongst property buyers in urban environments such as cities and metropoles due to the  increased security and lifestyle improvements offered. Garden design and layout requirements are prescribed in architectural guidelines compiled by the homeowners associations of these communities. Garden footprint area in gated community homes is of importance to researchers and planners, because of the influence on water use. This study used a quantitative approach to evaluate the spatial data of garden footprint area as a percentage of total plot area for 1 813 gated community homes in different regions of South Africa. The research reviewed how garden footprint area is prescribed and how it is applied in gated community homes. The impact of garden footprint area on water use was also analysed. The results were compared to relevant information lifted from specific architectural design guidelines developed for each gated community. Data from 11 gated communities were analysed and the average garden footprint area was found to be 36% of the total plot area. Gated community homes with a garden area smaller than 100 m2 were found to have limited influence on monthly water consumption, while the water use of gated community homes with a larger garden footprint area increased proportionally with garden footprint area. The seasonal fluctuation of water use is illustrative of garden irrigation and other outdoor water use. The results provided useful input for incorporation in outdoor water use modelling of gated  community homes Keywords: garden irrigation household water consumption plot are

Anti-cancer effects and mechanism of actions of aspirin analogues in the treatment of glioma cancer

INTRODUCTION: In the past 25 years only modest advancements in glioma treatment have been made, with patient prognosis and median survival time following diagnosis only increasing from 3 to 7 months. A substantial body of clinical and preclinical evidence has suggested a role for aspirin in the treatment of cancer with multiple mechanisms of action proposed including COX 2 inhibition, down regulation of EGFR expression, and NF-κB signaling affecting Bcl-2 expression. However, with serious side effects such as stroke and gastrointestinal bleeding, aspirin analogues with improved potency and side effect profiles are being developed. METHOD: Effects on cell viability following 24 hr incubation of four aspirin derivatives (PN508, 517, 526 and 529) were compared to cisplatin, aspirin and di-aspirin in four glioma cell lines (U87 MG, SVG P12, GOS – 3, and 1321N1), using the PrestoBlue assay, establishing IC50 and examining the time course of drug effects. RESULTS: All compounds were found to decrease cell viability in a concentration and time dependant manner. Significantly, the analogue PN517 (IC50 2mM) showed approximately a twofold increase in potency when compared to aspirin (3.7mM) and cisplatin (4.3mM) in U87 cells, with similar increased potency in SVG P12 cells. Other analogues demonstrated similar potency to aspirin and cisplatin. CONCLUSION: These results support the further development and characterization of novel NSAID derivatives for the treatment of glioma

Garden footprint area and water use of gated communities in South Africa

  Gated community homes in South Africa are popular amongst property buyers in urban environments such as cities and metropoles due to the increased security and lifestyle improvements offered. Garden design and layout requirements are prescribed in architectural guidelines compiled by the homeowners associations of these communities. Garden footprint area in gated community homes is of importance to researchers and planners, because of the influence on water use. This study used a quantitative approach to evaluate the spatial data of garden footprint area as a percentage of total plot area for 1 813 gated community homes in different regions of South Africa. The research reviewed how garden footprint area is prescribed and how it is applied in gated community homes. The impact of garden footprint area on water use was also analysed. The results were compared to relevant information lifted from specific architectural design guidelines developed for each gated community. Data from 11 gated communities were analysed and the average garden footprint area was found to be 36% of the total plot area. Gated community homes with a garden area smaller than 100 m2 were found to have limited influence on monthly water consumption, while the water use of gated community homes with a larger garden footprint area increased proportionally with garden footprint area. The seasonal fluctuation of water use is illustrative of garden irrigation and other outdoor water use. The results provided useful input for incorporation in outdoor water use modelling of gated community homes

Flow cytometric analysis of the oxidative status in human peripheral blood mononuclear cells of workers exposed to welding fumes

Flow cytometry is a simple analytical technique that identifies, counts, and characterizes cells. The oxidative status of cells is influenced by many exogenous sources, such as occupational exposure to welding fumes. This study evaluated flow cytometry as a method to determine the oxidative status of male welders (n = 15) occupationally exposed to welding fumes. Flow cytometric analysis of reactive oxygen species (ROS) was carried out in peripheral blood mononuclear cells (PBMC) by using the probe 2, 7-dichlorodihydrofluorescein diacetate (DCFH-DA). Lipid peroxidation was measured by the decrease of fluor-DHPE fluorescence and intracellular glutathione (GSH) levels by using mercury orange. All of the parameters were also observed under a confocal microscope. The oxidative stress ratio was calculated from the oxidative damage and the antioxidant capacity to give an accurate account of the cellular oxidative status. ROS and lipid peroxidation levels were elevated by ∼ 87% and ∼ 96%, respectively, and GSH levels lowered ∼ 96% in PBMC of workers exposed to welding fumes compared with non-exposed controls. The oxidative stress ratio was significantly higher (p < 0.001) in the exposed group. Flow cytometry can be useful for the measurement of cellular oxidative stress in somatic cells of workers exposed to welding fumes and other occupational settings. Calculating an oxidative stress index may be useful in predicting disease outcomes and whether preventative control measures are efficien