Microbial community dynamics in diesel waste biodegradation using sequencing batch bioreactor operation mode (SBR)

The dynamic of molecular microbial community during diesel waste biodegradation was investigated. The waste was treated in bioreactors operated in sequencing batch operation mode (SBR) in four cycles of 72 h, using optimized setpoints (pH, initial waste load, C:N ratio, aeration). Optimal conditions allowed the system to reach biodegradation of 53.3, 96.0, 76.2 and 75.0% at the end of cycles one, two three and four, respectively. Oxygen uptake rate (OUR) indicated increases in microbial activity from cycle one to cycle two (124.9 to 252.9 mgO2/L/h) and decreases in cycles three and four (120.4 to 108.8 mgO2/L/h, respectively). Investigations of microbial diversity showed changes in the microbial community members at the end of the cycle one. Significant reductions in the relative ecotoxicity were observed beginning with cycle two, and the reductions extended until the end of process. The SBR operation mode proved to be an efficient method for treating the diesel waste, and the process allowed for relevant reductions in the hydrocarbon content of the waste along with an increase in its environmental quality. Changes in the microbial members are evidence of the synergistic action of the microbiota in the process.Key words: Microbial diversity, denaturing gradient gel electrophoresis, sequencing batch, biodegradation

EVALUATION OF COSTS OF TWO HARVESTER MODELS IN THE CUT OF Eucalyptus

O presente estudo teve o objetivo de realizar a avalia\ue7\ue3o de custos de dois modelos de harvester, na colheita de madeira de eucalipto. A pesquisa avaliou a derrubada e o processamento da \ue1rvore, com idade de 6 anos, no sistema de toras curtas, para dois modelos de harvester, em \ue1reas localizadas nos munic\uedpios de Concei\ue7\ue3o da Barra \u2013 ES e Caravelas \u2013 BA. Avaliaram-se dois modelos de harvester, PC-228 SHO e PC-200 LC, da marca Komatsu. As informa\ue7\uf5es foram coletadas durante um per\uedodo de 6 meses, que compreende os meses de mar\ue7o a agosto de 2010, pelo m\ue9todo de censo. Calcularam-se os custos operacionais dos dois modelos de harvester, pelo m\ue9todo cont\ue1bil, o qual utiliza valores estimados em reais. Realizou-se ainda a an\ue1lise de sensibilidade de custos para os elementos que mais contribuem com o custo final. Obteve-se o custo operacional para os modelos PC 200 e PC 228 de R$156,95 e R$ 168,84 por hora efetiva, respectivamente. Em rela\ue7\ue3o aos custos operacionais totais, os custos mais significativos foram: combust\uedvel, manuten\ue7\ue3o e reparos e deprecia\ue7\ue3o, com 24,41%, 22,39% e 19,08%, respectivamente. Na an\ue1lise de sensibilidade, simulando uma situa\ue7\ue3o em que a empresa consiga uma economia real de 10% em cada um desses itens, a mesma poder\ue1 obter uma redu\ue7\ue3o no custo de produ\ue7\ue3o em 7%, para as duas m\ue1quinas avaliadas.The present study aimed to carry through the evaluation of costs of two models of harvester in the wood harvesting of Eucalyptus wood. The research evaluated the 6-year old tree fallen and processing, in the cut-to-length system for two models of harvester, in areas located in the cities of Concei\ue7\ue3o da Barra, Esp\uedrito Santo state and Caravelas, Bahia state. It was evaluated the models of harvester PC-228 SHO and PC-200 LC, of the Komatsu Mark. The information was collected during a period of 6 months, from March to August 2010, by the method of census. It was calculated the operational costs of the two models of harvester, for the countable method, which uses values in \u2018Reais\u2019, the Brazilian currency. It was conducted further analysis of cost sensitivity for the elements that contribute most to the final cost. We obtained the operating cost models for PC 200 and PC 228 of R$156,95 and R$ 168,84 per effective hour, respectively. In relation with the total operational costs, the most significant costs were: fuel, maintenance, and repairs/ depreciation, with 24.41%, 22.39% and 19.08%, respectively. In the sensitivity analysis, simulating a situation where the company gets a real economy of 10% in each one of these items, the same one will be able to get a reduction in the cost of production in 7%, for the two evaluated machines

Alcaligenes faecalis associated with Mimosa calodendron rizhosphere assist plant survival in arsenic rich soils.

The ferruginous rupestrian grasslands (FRG) in the Iron Quadrangle (IQ) are ecosystems characterized by rocky soils with reduced availability of water and nutrients, but high levels of metals. In order to comprehend the interference of microorganisms on the adaptive process of endemic plant Mimosa calodendrum (Fabaceae), bacteria associated with its roots and rhizosphere were isolated. Fourteen isolates were obtained and subsequently grown in the presence of different concentrations of arsenic (As) species. The isolate Mc250, an Alcaligenes faecalis strain, resisted to 10 mM of As (III) and 800 mM of As (V). In the presence of this strain, atomic spectrometer detected a reduction of 55% for As (III) and 72% for As (V) respectively in 10 mM and 500 mM solution. Scanning electron microscopy of this isolate demonstrated morphological modification and EDX spectroscopy revealed the presence of both As species adsorbed on the membrane, justifying the removal observed in the in vitro assays. To validate this potential removal of As in vivo, tomato plants were used as grown model in the presence and absence of A. faecalis in soil previously contaminated with 5 mM of As (III). After 14 days, plants from contaminated soil had their growth improved when compared to untreated control plants. All these results suggest for the first time that plant-associated bacteria from FRG-IQ present potential for soil rhizoremediation and may benefit the adaptive processes of plants in extreme environments including application in recovering degraded areas

Post-injection delirium/sedation syndrome in patients with schizophrenia treated with olanzapine long-acting injection, I: analysis of cases

<p>Abstract</p> <p>Background</p> <p>An advance in the treatment of schizophrenia is the development of long-acting intramuscular formulations of antipsychotics, such as olanzapine long-acting injection (LAI). During clinical trials, a post-injection syndrome characterized by signs of delirium and/or excessive sedation was identified in a small percentage of patients following injection with olanzapine LAI.</p> <p>Methods</p> <p>Safety data from all completed and ongoing trials of olanzapine LAI were reviewed for possible cases of this post-injection syndrome. Descriptive analyses were conducted to characterize incidence, clinical presentation, and outcome. Regression analyses were conducted to assess possible risk factors.</p> <p>Results</p> <p>Based on approximately 45,000 olanzapine LAI injections given to 2054 patients in clinical trials through 14 October 2008, post-injection delirium/sedation syndrome occurred in approximately 0.07% of injections or 1.4% of patients (30 cases in 29 patients). Symptomatology was consistent with olanzapine overdose (e.g., sedation, confusion, slurred speech, altered gait, or unconsciousness). However, no clinically significant decreases in vital signs were observed. Symptom onset ranged from immediate to 3 to 5 hours post injection, with a median onset time of 25 minutes post injection. All patients recovered within 1.5 to 72 hours, and the majority continued to receive further olanzapine LAI injections following the event. No clear risk factors were identified.</p> <p>Conclusions</p> <p>Post-injection delirium/sedation syndrome can be readily identified based on symptom presentation, progression, and temporal relationship to the injection, and is consistent with olanzapine overdose following probable accidental intravascular injection of a portion of the olanzapine LAI dose. Although there is no specific antidote for olanzapine overdose, patients can be treated symptomatically as needed. Special precautions include use of proper injection technique and a post-injection observation period.</p> <p>Trial Registration</p> <p>ClinicalTrials.gov ID; URL: <url>http://http//www.clinicaltrials.gov/</url>: NCT00094640, NCT00088478, NCT00088491, NCT00088465, and NCT00320489.</p

Dengue Virus Type 4 Phylogenetics in Brazil 2011: Looking beyond the Veil

Dengue Fever and Dengue Hemorrhagic Fever are diseases affecting approximately 100 million people/year and are a major concern in developing countries. In the present study, the phylogenetic relationship of six strains of the first autochthonous cases of DENV-4 infection occurred in Sao Paulo State, Parana State and Rio Grande do Sul State, Brazil, 2011 were studied. Nucleotide sequences of the envelope gene were determined and compared with sequences representative of the genotypes I, II, III and Sylvatic for DEN4 retrieved from GenBank. We employed a Bayesian phylogenetic approach to reconstruct the phylogenetic relationships of Brazilian DENV-4 and we estimated evolutionary rates and dates of divergence for DENV-4 found in Brazil in 2011. All samples sequenced in this study were located in Genotype II. The studied strains are monophyletic and our data suggest that they have been evolving separately for at least 4 to 6 years. Our data suggest that the virus might have been present in the region for some time, without being noticed by Health Surveillance Services due to a low level of circulation and a higher prevalence of DENV-1 and DENV- 2

Differential Geometry for Model Independent Analysis of Images and Other Non-Euclidean Data: Recent Developments

This article provides an exposition of recent methodologies for nonparametric analysis of digital observations on images and other non-Euclidean objects. Fr\'echet means of distributions on metric spaces, such as manifolds and stratified spaces, have played an important role in this endeavor. Apart from theoretical issues of uniqueness of the Fr\'echet minimizer and the asymptotic distribution of the sample Fr\'echet mean under uniqueness, applications to image analysis are highlighted. In addition, nonparametric Bayes theory is brought to bear on the problems of density estimation and classification on manifolds

A Constructive Fuzzy NGE Learning System

This paper presents FNGE, a learning system based on a fuzzy version of the NGE theory, describes its main modules and discusses some empirical results from its use in public domains. 2 FNGE Prototype Syste

Organic matter cycling in a neotropical reservoir: effects of temperature and experimental conditions

Aim: This study reports a comparison between decomposition kinetics of detritus derived from two macrophyte species (Polygonum lapathifolium L.: Polygonaceae; Eichhornia azurea (Sw.) Kunth.: Pontederiaceae) growing in a neotropical reservoir (Brazil), under laboratory and field conditions, in order to assess hypotheses on the main differences in factors affecting organic matter cycling, including the effect of temperature. Methods: Plant and water samples were collected from the reservoir in August 2009. In field incubation mass loss was assessed using a litter bag technique and in the laboratory the decay was followed using a decomposition chamber maintained under controlled conditions (i.e. in the dark, at 15 °C and 25 °C). A kinetic model was adopted to explain and compare the organic matter decay, ANOVA (Repeated Measures) testing was used to describe the differences between the treatments and a linear correlation was used to compare in situ and in vitro experiments. Results: The mass decay was faster in natural conditions with rapid release of the labile-soluble portion. The simulated values of mineralization rates of dissolved organic matter and refractory organic matter were rapid in high temperatures (25 °C). The high Q10 results (mainly for E. azurea), and experimental conditions, and outcomes of ANOVA testing indicate the temperature variation (10 °C) influence the rates of mass decay. Conclusions: The results suggested rapid organic matter cycling in warm months (from October to December) supporting the microbial loop. Although the particulate organic matter losses are high in field conditions the results are of the same magnitude in both conditions suggesting an equivalence of the mass decay kinetic