Integrated process production and extraction of the fibrinolytic protease from Bacillus sp. UFPEDA 485

Fibrinolytic proteases are enzymes that degrade fibrin; these enzymes are a promising alternative for thrombolytic therapy, and microorganisms produce them. The aim of this study was to evaluate the optimum conditions for the integrated production and purification of fibrinolytic protease from Bacillus sp. UFPEDA 485. Extractive fermentation was carried out in a culture medium containing soybean flour and by adding polyethylene glycol (PEG) and Na2SO4 according to a 23 experimental design. In all assays, the enzyme preferentially partitioned to the bottom phase (K < 1), with an optimum activity of 835 U ml−1 in the bottom phase (salt-rich phase). The best conditions for extractive fermentation were obtained with 18 % PEG 8000 and 13 % Na2SO4. Characterization showed that it is a metalloprotease, as a strong inhibition—residual activity of 3.13 %—occurred in the presence of ethylenediaminetetraacetic acid. It was also observed that enzymatic activity was stimulated in the presence of ions: CaCl2 (440 %), MgCl2 (440 %), FeSO4 (268 %), and KCl (268 %). The obtained results indicate that the use of a low-cost substrate and the integration of fermentation with an aqueous two-phase system extraction may be an interesting alternative for the production of fibrinolytic protease.The authors thank CAPES (National Council for the Improvement of Higher Education) for the scholarship and CNPq (National Counsel of Technological and Scientific Development) and RENORBIO for the financial support

Obtenção de café com qualidade no Acre.

No Estado do Acre, o desenvolvimento da cafeicultura é fundamental para a geração de emprego e renda, aumento de receita estadual, redução de importações, melhoria das condições de vida dos agricultores e fixação do homem à terra. Fatores como espécies e variedades de cultivo, tratos culturais (adubação, capinas e controle de pragas e doenças), condições climáticas e, principalmente, cuidados nas operações agrícolas e pós-colheita (secagem, armazenamento, torração, moagem e preparo da infusão) interferem, em muito, na qualidade final da bebida de café. Este trabalho foi realizado com a finalidade de detectar falhas e promover melhorias nas técnicas de colheita, secagem, armazenamento e beneficiamento do café, de forma que todos os setores ligados à cadeia produtiva na cultura Estado do Acre envolvam-se para aumentar a produção de cafés de qualidade e a rentabilidade econômica dessa atividade agrícola.bitstream/CPAF-AC/3997/1/cirtec34.pd

Effects of a high-energy diet on oocyte quality and in vitro embryo production in Bos indicus and Bos taurus cows.

201

Evolução da maturação e características físicoquímicas da videira "BRS Vitória" em diferentes épocas de poda.

Na região noroeste paulista são realizadas duas podas no mesmo ano, a primeira de produção, realizada de fevereiro a junho, e a segunda para formação, realizada entre junho a novembro. O período de colheita se estende de julho a novembro, diferentemente de outras regiões produtoras, o que beneficia os produtores na comercialização da uva

Effects of a Competitive Season on Autonomic Heart Rate Modulation in Field Soccer Athletes

International Journal of Exercise Science 12(2): 1198-1205, 2019. The physical demands of soccer combined with the rigor of the competitive season may have a substantial impact on autonomic modulation in field soccer athletes. The number of sudden death cases associated with soccer may be related to the physical training required to maintain performance and fitness, minimal time for recovery, and recurrent game participation. It is possible to identify individuals at risk of cardiovascular events by measuring heart rate variability (HRV), which is an indirect method for assessing autonomic activity. Therefore, the objective of this study was to analyze HRV before and after a period of field soccer competition. We evaluated 17 healthy male professional field soccer athletes and 12 untrained controls. The HRV was analyzed during supine rest before and after a period of field soccer competition. The following parameters were evaluated: interval R wave variation (RR), standard deviation of normal–normal intervals (SDNN), Root-mean-square successive difference (RMSSD), low frequency component (LF), high-frequency component (HF) and sympathovagal balance (LF/HF). Results indicated that the RR (p\u3c .05, ES: 2.77), SDNN (p \u3c .05, ES: 1.70), LF (p\u3c .05, ES: 1.86), HF (pp\u3c .05, ES: 0.89) all decreased after the competition in the professional athletes with no change observed in the control group. In conclusion, the data suggest that a soccer competition negatively influences the autonomic regulation of heart rate

Caracterização de linhagens elite de feijão carioca quanto à reação ao mofo-branco em campo e em ambiente controlado.

O objetivo deste trabalho foi caracterizar linhagens pré-comerciais de feijão carioca quanto à reação ao mofo-branco em campo e em ambiente controlado, para identificar fontes de resistência a serem exploradas pelo Programa de Melhoramento de Feijão da Embrapa e seus parceiros

Globular cluster systems in fossil groups: NGC6482, NGC1132 and ESO306-017

We study the globular cluster (GC) systems in three representative fossil group galaxies: the nearest (NGC6482), the prototype (NGC1132) and the most massive known to date (ESO306-017). This is the first systematic study of GC systems in fossil groups. Using data obtained with the Hubble Space Telescope Advanced Camera for Surveys in the F475W and F850LP filters, we determine the GC color and magnitude distributions, surface number density profiles, and specific frequencies. In all three systems, the GC color distribution is bimodal, the GCs are spatially more extended than the starlight, and the red population is more concentrated than the blue. The specific frequencies seem to scale with the optical luminosities of the central galaxy and span a range similar to that of the normal bright elliptical galaxies in rich environments. We also analyze the galaxy surface brightness distributions to look for deviations from the best-fit S\'ersic profiles; we find evidence of recent dynamical interaction in all three fossil group galaxies. Using X-ray data from the literature, we find that luminosity and metallicity appear to correlate with the number of GCs and their mean color, respectively. Interestingly, although NGC6482 has the lowest mass and luminosity in our sample, its GC system has the reddest mean color, and the surrounding X-ray gas has the highest metallicity.Comment: 16 pages, 13 figures. Accepted for publication in A&