53 research outputs found
ΠΠ°ΡΡΠΎΡΡΠ²Π°Π½Π½Ρ Π·Π²ΠΎΡΠΎΡΠ½ΠΈΡ Π·Π°Π»Π΅ΠΆΠ½ΠΎΡΡΠ΅ΠΉ Ρ ΠΌΠ°ΡΠ΅ΠΌΠ°ΡΠΈΡΠ½ΠΈΡ ΠΌΠΎΠ΄Π΅Π»ΡΡ ΡΠΊΠ»Π°Π΄Π½ΠΈΡ ΠΎΠ±βΡΠΊΡΡΠ² ΡΠ° ΡΠΈΡΡΠ΅ΠΌ
ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ΠΎ ΠΌΠ΅ΡΠΎΠ΄ ΠΏΠΎΠ±ΡΠ΄ΠΎΠ²ΠΈ Π°ΠΏΡΠΎΠΊΡΠΈΠΌΡΡΡΠΈΡ
ΠΏΠΎΠ»ΡΠ½ΠΎΠΌΡΠ°Π»ΡΠ½ΠΈΡ
ΡΡΠ½ΠΊΡΡΠΉ Π±Π°Π³Π°ΡΡΠΎΡ
Π·ΠΌΡΠ½Π½ΠΈΡ
, ΡΠΊΠΈΠΉ Π·Π°ΡΠ½ΠΎΠ²Π°Π½ΠΎ Π½Π° Π²ΠΈΠΊΠΎΡΠΈΡΡΠ°Π½Π½Ρ Π² ΠΏΠΎΠ»ΡΠ½ΠΎΠΌΠ°Ρ
Π²ΡΠ΄βΡΠΌΠ½ΠΈΡ
ΡΡΠ΅ΠΏΠ΅Π½ΡΠ² ΡΠ° Π·Π°ΡΡΠΎΡΡΠ²Π°Π½Π½Ρ Π΄ΠΎ ΠΏΠΎΠ»ΡΠ½ΠΎΠΌΡΠ² ΠΎΠ±ΠΌΠ΅ΠΆΠ΅Π½Π½Ρ Π½Π° ΡΡΠΌΠ°ΡΠ½Ρ Π²Π΅Π»ΠΈΡΠΈΠ½Ρ ΡΡΡΠΏΠ΅Π½Ρ Π΄ΠΎΠ±ΡΡΠΊΡ Π·ΠΌΡΠ½Π½ΠΈΡ
. ΠΠ°ΠΏΡΠΎΠΏΠΎΠ½ΠΎΠ²Π°Π½ΠΎ Π²ΠΈΠΊΠΎΡΠΈΡΡΠ°Π½Π½Ρ ΡΡΡΠ°ΡΠ½ΠΎΡ ΡΡΠ½ΠΊΡΡΡ Π½Π° ΠΊΡΠ»ΡΠΊΡΡΡΡ ΡΠ»Π΅Π½ΡΠ² ΠΏΠΎΠ»ΡΠ½ΠΎΠΌΠ°. ΠΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΈΠΌ ΡΠ»ΡΡ
ΠΎΠΌ ΠΎΡΡΠΈΠΌΠ°Π½ΠΎ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½Ρ Π²Π΅Π»ΠΈΡΠΈΠ½Ρ ΠΊΠΎΠ΅ΡΡΡΡΡΠ½ΡΠ° Π·Π°ΠΏΡΠΎΠΏΠΎΠ½ΠΎΠ²Π°Π½ΠΎΡ ΡΡΡΠ°ΡΠ½ΠΎΡ ΡΡΠ½ΠΊΡΡΡ.ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ ΠΌΠ΅ΡΠΎΠ΄ ΠΏΠΎΡΡΡΠΎΠ΅Π½ΠΈΡ Π°ΠΏΠΏΡΠΎΠΊΡΠΈΠΌΠΈΡΡΡΡΠΈΡ
ΠΏΠΎΠ»ΠΈΠ½ΠΎΠΌΠΈΠ°Π»ΡΠ½ΡΡ
ΡΡΠ½ΠΊΡΠΈΠΉ ΠΌΠ½ΠΎΠ³ΠΈΡ
ΠΏΠ΅ΡΠ΅ΠΌΠ΅Π½Π½ΡΡ
, ΠΎΡΠ½ΠΎΠ²Π°Π½Π½ΡΠΉ Π½Π° ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠΈ Π² ΠΏΠΎΠ»ΠΈΠ½ΠΎΠΌΠ°Ρ
ΠΎΡΡΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΡΡ
ΡΡΠ΅ΠΏΠ΅Π½Π΅ΠΉ ΠΈ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠΈ ΠΊ ΠΏΠΎΠ»ΠΈΠ½ΠΎΠΌΠ°ΠΌ ΠΎΠ³ΡΠ°Π½ΠΈΡΠ΅Π½ΠΈΡ Π½Π° ΡΡΠΌΠΌΠ°ΡΠ½ΡΡ Π²Π΅Π»ΠΈΡΠΈΠ½Ρ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ ΠΏΡΠΎΠΈΠ·Π²Π΅Π΄Π΅Π½ΠΈΡ ΠΏΠ΅ΡΠ΅ΠΌΠ΅Π½Π½ΡΡ
. ΠΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½ΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΡΡΠ°ΡΠ½ΠΎΠΉ ΡΡΠ½ΠΊΡΠΈΠΈ Π½Π° ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΡΠ»Π΅Π½ΠΎΠ² ΠΏΠΎΠ»ΠΈΠ½ΠΎΠΌΠ°. ΠΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΠΌ ΠΏΡΡΠ΅ΠΌ ΠΏΠΎΠ»ΡΡΠ΅Π½Π° ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½Π°Ρ Π²Π΅Π»ΠΈΡΠΈΠ½Π° ΠΊΠΎΡΡΡΠΈΡΠΈΠ΅Π½ΡΠ° ΠΏΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½Π½ΠΎΠΉ ΡΡΡΠ°ΡΠ½ΠΎΠΉ ΡΡΠ½ΠΊΡΠΈΠΈ.A method of constructing approximating polynomials functions of many variables, based on the use of the negative degrees in polynomials and the application of the limitation on the total value of the product variable to polynoms is presented. The usage of the penalty function for the number of polynomial members is suggested. The optimum value of the proposed penalty functions coefficient is experimentally obtained
Real-world comparison of the effects of etanercept and adalimumab on well-being in non-systemic juvenile idiopathic arthritis: a propensity score matched cohort study
Background: Etanercept (ETN) and adalimumab (ADA) are considered equally efective biologicals in the treatβ
ment of arthritis in juvenile idiopathic arthritis (JIA) but no studies have compared their impact on patient-reported
well-being. The objective of this study was to determine whether ETN and ADA have a diferential efect on patientreported well-being in non-systemic JIA using real-world data.
Methods: Biological-naive patients without a history of uveitis were selected from the international Pharmachild
registry. Patients starting ETN were matched to patients starting ADA based on propensity score and outcomes were
collected at time of therapy initiation and 3β12 months afterwards. Primary outcome at follow-up was the improveβ
ment in Juvenile Arthritis Multidimensional Assessment Report (JAMAR) visual analogue scale (VAS) well-being score
from baseline. Secondary outcomes at follow-up were decrease in active joint count, adverse events and uveitis
events. Outcomes were analyzed using linear and logistic mixed efects models.
Results: Out of 158 eligible patients, 45 ETN starters and 45 ADA starters could be propensity score matched resultβ
ing in similar VAS well-being scores at baseline. At follow-up, the median improvement in VAS well-being was 2 (interβ
quartile range (IQR): 0.0 β 4.0) and scores were signifcantly better (P=0.01) for ETN starters (median 0.0, IQR: 0.0 β 1.0)
compared to ADA starters (median 1.0, IQR: 0.0 β 3.5). The estimated mean diference in VAS well-being improvement
from baseline for ETN versus ADA was 0.89 (95% CI: -0.01 β 1.78; P=0.06). The estimated mean diference in active
joint count decrease was -0.36 (95% CI: -1.02 β 0.30; P=0.28) and odds ratio for adverse events was 0.48 (95% CI: 0.16
β1.44; P=0.19). One uveitis event was observed in the ETN group.
Conclusions: Both ETN and ADA improve well-being in non-systemic JIA. Our data might indicate a trend towards a
slightly stronger efect for ETN, but larger studies are needed to confrm this given the lack of statistical signifcance
Understanding inflammation in juvenile idiopathic arthritis : How immune biomarkers guide clinical strategies in the systemic onset subtype
The translation of basic insight in immunological mechanisms underlying inflammation into clinical practice of inflammatory diseases is still challenging. Here we describe how - through continuous dialogue between bench and bedside - immunological knowledge translates into tangible clinical use in a complex inflammatory disease, juvenile idiopathic arthritis (JIA). Systemic JIA (sJIA) is an autoinflammatory disease, leading to the very successful use of IL-1 antagonists. Further immunological studies identified new immune markers for diagnosis, prediction of complications, response to and successful withdrawal of therapy. Myeloid Related Protein (MRP)-8, MRP-14, S100-A12 and Interleukin-18 are already used daily in clinic as markers for active sJIA. For non-sJIA subtypes, HLA-B27, antinuclear-antibodies, rheumatoid factor, erythrocyte sedimentation rate and C-reactive protein are still used for classification, prognosis or active disease. MRP-8, MRP-14 and S100-A12 are now under study for clinical practice. We believe that with biomarkers, algorithms can soon be designed for the individual risk of disease, complications, damage, prediction of response to, and successful withdrawal of therapy. In that way, less time will be lost and less pain will be suffered by the patients. In this review we describe the current status of immunological biomarkers used in diagnosis and treatment of JIA
Clinical Juvenile Arthritis Disease Activity Score proves to be a useful tool in treat-to-target therapy in juvenile idiopathic arthritis
OBJECTIVES: To assess if the Juvenile Arthritis Disease Activity Score (JADAS71) could be used to correctly identify patients with juvenile idiopathic arthritis (JIA) in need of antitumour necrosis factor therapy (anti-TNF) therapy 3 and 6 months after start of methotrexate (MTX). METHODS: Monocentric retrospective cohort study from 2011 to 2015 analysing all patients with oligoarticular JIA (OJIA) (n=39) and polyarticular course JIA (PJIA) (n=74) first starting MTX. Three and 6βmonths after MTX start, clinical and laboratory features and the 2011 American College of Rheumatology (ACR) JIA treatment recommendations (ACR clinical practice guideline (ACR-CPG)) were compared between groups starting and not starting anti-TNF therapy. The sensitivity and specificity of the ACR-CPG, JADAS71 and the clinical JADAS to identify non-responders after 12 months were calculated. RESULTS: Physicians escalated patients with significantly higher physician global assessment, clinical JADAS (cJADAS) and patient Visual Analogue Scale (VAS). The decision not to escalate was correct in 70%-75% as shown by MTX response. The implementation of the ACR-CPG would increase the current anti-TNF use from 12% to 65%. The use of (c)JADAS in identifying patients in need of anti-TNF therapy outperformed the ACR-CPG with a much higher sensitivity, specificity and accuracy. The cJADAS threshold for treatment escalation at month 3 and 6 was >5βand >3 for OJIA and >7βand >4 for PJIA, respectively. The performance of the cJADAS decreased when the patient VAS contribution to the total score was restricted and overall did not improve by adding the erythrocyte sedimentation rate. CONCLUSIONS: The cJADAS identifies patients in need of anti-TNF and is a user-friendly tool ready to be used for treat to target in JIA. The patient VAS is a critical item in the cJADAS for the decision to escalate to anti-TNF
The human microbiome and juvenile idiopathic arthritis
Juvenile idiopathic arthritis (JIA) is the most common rheumatic disease in childhood. The pathogenesis of JIA is thought to be the result of a combination of host genetic and environmental triggers. However, the precise factors that determine one's susceptibility to JIA remain to be unravelled. The microbiome has received increasing attention as a potential contributing factor to the development of a wide array of immune-mediated diseases, including inflammatory bowel disease, type 1 diabetes and rheumatoid arthritis. Also in JIA, there is accumulating evidence that the composition of the microbiome is different from healthy individuals. A growing body of evidence indeed suggests that, among others, the microbiome may influence the development of the immune system, the integrity of the intestinal mucosal barrier, and the differentiation of T cell subsets. In turn, this might lead to dysregulation of the immune system, thereby possibly playing a role in the development of JIA. The potential to manipulate the microbiome, for example by faecal microbial transplantation, might then offer perspectives for future therapeutic interventions. Before we can think of such interventions, we need to first obtain a deeper understanding of the cause and effect relationship between JIA and the microbiome. In this review, we discuss the existing evidence for the involvement of the microbiome in JIA pathogenesis and explore the potential mechanisms through which the microbiome may influence the development of autoimmunity in general and JIA specifically
Increased autophagy contributes to the inflammatory phenotype of juvenile idiopathic arthritis synovial fluid T cells
Objectives: JIA is an autoimmune disease involving disturbed T-cell homeostasis, marked by highly activated effector T cells. Autophagy, a lysosomal degradation pathway, is crucial for maintaining cellular homeostasis by regulating the survival, differentiation and function of a large variety of cells, including T cells. The aim of this study was to examine the rate of autophagy in JIA T cells and to investigate the effect of inhibition of autophagy on the inflammatory phenotype of JIA T cells. Methods: Autophagy-related gene expression was analysed in CD4+ T cells from the SF of JIA patients and healthy controls using RNA sequencing. Autophagy was measured by flow cytometry and western blot. The effect of inhibition of autophagy, using HCQ, on the cellular activation status was analysed using flow cytometry and multiplex immunoassay. Results: Autophagy was increased in T cells derived from the site of inflammation compared with cells from the peripheral blood of patients and healthy controls. This increase in autophagy was not induced by JIA SF, but is more likely to be the result of increased cellular activation. Inhibition of autophagy reduced proliferation, cytokine production and activation marker expression of JIA SF-derived CD4+ T cells. Conclusion: These data indicate that autophagy is increased in JIA SF-derived T cells and that targeting autophagy could be a promising therapeutic strategy to restore the disrupted T-cell homeostasis in JIA
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