25 research outputs found
Dysregulation of Transcription Factor Networks Unveils Different Pathways in Human Papillomavirus 16-Positive Squamous Cell Carcinoma and Adenocarcinoma of the Uterine Cervix
Squamous cell carcinoma (SCC) and adenocarcinoma (ADC) are the most common
histological types of cervical cancer (CC). The worse prognosis of ADC cases highlights
the need for better molecular characterization regarding differences between these
CC types. RNA-Seq analysis of seven SCC and three ADC human papillomavirus
16-positive samples and the comparison with public data from non-tumoral human
papillomavirus-negative cervical tissue samples revealed pathways exclusive to each
histological type, such as the epithelial maintenance in SCC and the maturity-onset
diabetes of the young (MODY) pathway in ADC. The transcriptional regulatory network
analysis of cervical SCC samples unveiled a set of six transcription factor (TF) genes
with the potential to positively regulate long non-coding RNA genes DSG1-AS1,
CALML3-AS1, IGFL2-AS1, and TINCR. Additional analysis revealed a set of MODY TFs
regulated in the sequence predicted to be repressed bymiR-96-5p ormiR-28-3p in ADC.
These microRNAs were previously described to target LINC02381, which was predicted
to be positively regulated by two MODY TFs upregulated in cervical ADC. Therefore, we
hypothesize LINC02381might act by decreasing the levels ofmiR-96-5p andmiR-28-3p,
promoting the MODY activation in cervical ADC. The novel TF networks here described
should be explored for the development of more efficient diagnostic tools
The James Webb Space Telescope Mission
Twenty-six years ago a small committee report, building on earlier studies,
expounded a compelling and poetic vision for the future of astronomy, calling
for an infrared-optimized space telescope with an aperture of at least .
With the support of their governments in the US, Europe, and Canada, 20,000
people realized that vision as the James Webb Space Telescope. A
generation of astronomers will celebrate their accomplishments for the life of
the mission, potentially as long as 20 years, and beyond. This report and the
scientific discoveries that follow are extended thank-you notes to the 20,000
team members. The telescope is working perfectly, with much better image
quality than expected. In this and accompanying papers, we give a brief
history, describe the observatory, outline its objectives and current observing
program, and discuss the inventions and people who made it possible. We cite
detailed reports on the design and the measured performance on orbit.Comment: Accepted by PASP for the special issue on The James Webb Space
Telescope Overview, 29 pages, 4 figure
Aplicação das seqüências dos espaçadores internos transcritos do DNA ribossomal nuclear para estudos filogenéticos com o gênero Passiflora (Passifloraceae)
Resumo não disponível
Aplicação das seqüências dos espaçadores internos transcritos do DNA ribossomal nuclear para estudos filogenéticos com o gênero Passiflora (Passifloraceae)
Resumo não disponível
Evolução molecular Darwiniana nas proteínas relacionadas à patogênese (PRs) em plantas
Um total de 201 seqüências de DNA, de 50 espécies pertencentes a 32 gêneros e 12 famílias, foi investigado através do método da máxima verossimilhança para identificar, nas proteínas respectivas, possíveis códons nos quais estivesse ocorrendo seleção positiva. Foram considerados 15 tipos de proteínas relacionadas à patogênese (PR1-PR15), quanto a 14 modelos diferentes de seleção. Tanto quanto se possa avaliar, não há qualquer estudo disponível na literatura que tenha examinado de maneira homogênea tal número de seqüências de forma tão abrangente.A total of 210 DNA sequences from 50 species belonging to 32 genera and 12 families was investigated by the maximum likelihood method to identify, in the respective proteins, possible codons in which positive selection would be occurring. Fifteen types of pathogenesis related proteins (PR1 – PR15) were considered in conection with 14 different selection models. As far as can be ascertained, there is no study in the literature which had examined in a homogeneous and comprehensive way such a number of sequences
Evolução molecular Darwiniana nas proteínas relacionadas à patogênese (PRs) em plantas
Um total de 201 seqüências de DNA, de 50 espécies pertencentes a 32 gêneros e 12 famílias, foi investigado através do método da máxima verossimilhança para identificar, nas proteínas respectivas, possíveis códons nos quais estivesse ocorrendo seleção positiva. Foram considerados 15 tipos de proteínas relacionadas à patogênese (PR1-PR15), quanto a 14 modelos diferentes de seleção. Tanto quanto se possa avaliar, não há qualquer estudo disponível na literatura que tenha examinado de maneira homogênea tal número de seqüências de forma tão abrangente.A total of 210 DNA sequences from 50 species belonging to 32 genera and 12 families was investigated by the maximum likelihood method to identify, in the respective proteins, possible codons in which positive selection would be occurring. Fifteen types of pathogenesis related proteins (PR1 – PR15) were considered in conection with 14 different selection models. As far as can be ascertained, there is no study in the literature which had examined in a homogeneous and comprehensive way such a number of sequences
Patterns of molecular evolution in pathogenesis-related proteins
The genes encoding 13 classes of pathogenesis-related (PR) proteins were examined for positive selection using maximum-likelihood (ML) models of codon substitution. The study involved 194 sequences from 54 species belonging to 37 genera. Although the sizes of the sequences examined varied from 237 bp for PR12 to 1,110 bp for PR7, most classes (9 out of 13) contained sequences made up of more than 400 nucleotides. Signs of positive selection were obtained for sites in PR proteins 4, 6, 8, 9 and 15 using an ML-based Bayesian method and likelihood ratio tests. These results confirm the importance of positive selection in proteins related to defense mechanisms already observed in a wide array of organisms