Comparative Metabolomics Study of the Impact of Articaine and Lidocaine on the Metabolism of SH-SY5Y Neuronal Cells

Articaine (ATC) and lidocaine (LDC) are the local anesthetics (LAs) currently most employed in dentistry. Cases of paresthesia, reported more frequently for ATC, have raised concerns about their potential neurotoxicity, calling for further investigation of their biological effects in neuronal cells. In this work, the impact of ATC and LDC on the metabolism of SH-SY5Y cells was investigated through 1H NMR metabolomics. For each LA, in vitro cultured cells were exposed to concentrations causing 10 and 50% reductions in cell viability, and their metabolic intracellular and extracellular profiles were characterized. Most effects were common to ATC and LDC, although with varying magnitudes. The metabolic variations elicited by the two LAs suggested (i) downregulation of glycolysis and of glucose-dependent pathways (e.g., one-carbon metabolism and hexosamine biosynthetic pathway), (ii) disturbance of branched chain amino acids (BCAA) catabolism, (iii) downregulation of TCA cycle anaplerotic fueling and activation of alternative energy producing pathways, (iv) interference with choline metabolism and (v) lipid droplet build-up. Interestingly, LDC had a greater impact on membrane phospholipid turnover, as suggested by higher phosphatidylcholine to phosphocholine conversion. Moreover, LDC elicited an increase in triglycerides, whereas cholesteryl esters accumulated in ATC-exposed cells, suggesting a different composition and handling of lipid droplets.publishe

Alterações físicas e químicas de um Argissolo amarelo sob diferentes sistemas de uso e manejo

As alterações nas condições químicas do solo em virtude de diferentes sistemas de manejo com cana-de-açúcar podem causar modificações na dispersão de argila e no estado de agregação do solo. Estudaram-se os efeitos de diferentes sistemas de manejo com cana-de-açúcar nas propriedades físicas e químicas de um Argissolo Amarelo Coeso, selecionando-se quatro sistemas de uso e manejo, constituídos de uma área não irrigada, uma área irrigada, uma com aplicação de vinhaça e uma sob floresta nativa como condição original. Foram retiradas amostras de cada área a 0-0,2, 0,2-0,4 e 0,4-0,8 m de profundidade, para determinação das alterações nas propriedades físicas e químicas do solo. Os sistemas de manejo com cana-de-açúcar afetaram as propriedades químicas do solo, ocorrendo redução da capacidade de troca de cátions e aumento da condutividade elétrica, pH em água e concentração crítica de floculação. Essas alterações químicas resultaram numa dispersão maior de argila nos solos cultivados com cana-de-açúcar, reduzindo a estabilidade dos agregados em água

Use of cork granules as an effective sustainable material to clean-up spills of crude oil and derivatives

The use of cork granules for cleaning up crude oil or oil derivative spills and further oil recovery appears as a promising option due to their unique properties, which allow a high oil sorption capacity, low water pickup and excellent reuse. The present work reports the effect of oil viscosity on cork sorption capacity by using five types of oils (lubricating oil, 5.7 goil gcork-1; heavy oil, 4.2 goil gcork-1; light oil, 3.0 goil gcork-1; biodiesel, 2.6 goil gcork-1; and diesel, 2.0 goil gcork-1). The cork sorption capacity for light petroleum was also evaluated as a function of temperature and sorbent particle size. Additionally, improvements on oil recovery from cork sorbents by a mechanical compression process have been achieved as a result of a design of experiments (DOE) using the response surface methodology. Such statistical technique provided remarkable results in terms of cork sorbent reusability, as the oil sorption capacity was preserved after 30 cycles of sorption-squeezing steps. The sorbed oils could be removed from the sorbent surface, collected simply by squeezing the cork granules and further reused. The best operational region yielded near 80% oil recovery, using a cork mass of 8.85 g (particle size of 2.0-4.0 mm) loaded with 43.5 mL of lubricating oil, at 5.4 bar, utilising two compressions with a duration of 2 min each. Graphical abstract.publishe

Docetaxel and Lidocaine Co-Loaded (NLC-in-Hydrogel) Hybrid System Designed for the Treatment of Melanoma

Melanoma is the most aggressive skin carcinoma and nanotechnology can bring new options for its pharmacological treatment. Nanostructured lipid carriers (NLC) are ideal drug-delivery carriers for hydrophobic drugs, such as the antineoplastic docetaxel (DTX), and hybrid (NLC-in-hydrogel) systems are suitable for topical application. This work describes a formulation of NLCDTX in xanthan-chitosan hydrogel containing lidocaine (LDC) with anticancer and analgesia effects. The optimized nanoparticles encapsulated 96% DTX and rheological analysis revealed inherent viscoelastic properties of the hydrogel. In vitro assays over murine fibroblasts (NIH/3T3) and melanoma cells (B16-F10), human keratinocytes (HaCaT) and melanoma cells (SK-MEL-103) showed reduction of docetaxel cytotoxicity after encapsulation in NLCDTX and HGel-NLCDTX. Addition of LDC to the hybrid system (HGel-NLCDTX-LDC) increased cell death in tumor and normal cells. In vivo tests on C57BL/6J mice with B16-F10-induced melanoma indicated that LDC, NLCDTX, HGel-NLCDTX-LDC and NLCDTX + HGel-LDC significantly inhibited tumor growth while microPET/SPECT/CT data suggest better prognosis with the hybrid treatment. No adverse effects were observed in cell survival, weight/feed-consumption or serum biochemical markers (ALT, AST, creatinine, urea) of animals treated with NLCDTX or the hybrid system. These results confirm the adjuvant antitumor effect of lidocaine and endorse HGel-NLCDTX-LDC as a promising formulation for the topical treatment of melanoma

Aspectos morfológicos e influência do tamanho da semente na germinação do jambo-vermelho Morphological aspects and influence of the seed size in the germination of wax jambu

O jambo-vermelho (Syzygium malaccense (L.) Merryl et Perry), espécie pertencente à família Myrtaceae, possui frutos ricos em vitaminas, ferro e fósforo. Este trabalho teve por objetivos fornecer informações sobre aspectos morfológicos do fruto, semente e plântula e avaliar a influência do tamanho da semente na germinação dessa espécie. O fruto é uma baga piriforme, carnoso e indeiscente. O epicarpo é delgado, liso e avermelhado; o mesocarpo e o endocarpo são esbranquiçados e suculentos. Os frutos pesam, em média, 35,57g e medem 7,16cm de comprimento e 5,15cm de largura. As sementes são poliembriônicas e exalbuminosas, pesando, em média, 7,62g. Os cotilédones são maciços e esverdeados. A germinação é hipógea, e a emergência das plântulas inicia-se aos 36 dias após a semeadura. A raiz primária é longa e esbranquiçada. As raízes secundárias são curtas e filiformes. As sementes de maior tamanho são as mais vigorosas, não havendo diferença entre as de tamanho médio e pequeno. O estudo morfológico do jambo-vermelho pode ser utilizado para a identificação da espécie ou em relações ecológicas interespecíficas.<br>The fruits of wax jambu (Syzygium malaccense (L.) Merryl et Perry), species belonging to the Myrtaceae family, is a source of vitamins, iron and phosphorus. This work aimed to supply information on morphological aspects of the fruit, seed and seedling and to evaluate the influence of the seed size in the germination of this species. The fruit is a pear-shaped bacca, juicy and undeiscent. The epicarp is thin, smooth and redish; the mesocarp and the endocarp are whitish and succulent. The fruits weigh, in average, 35,57g and measure 7,16cm in length and 5,15cm in width. The seeds are polyembrionic and unalbuminous, weighing, in average, 7,62g. The cotyledons are massive and greenish. The germination is hypogeal and the emergency of the seedlings starts at 36 days after the sowing. The primary root is long and whitish. The secondary roots are short and filiform. The large seeds are most vigorous and it doesn't have difference between the seeds of medium and small size. The morphological study of the wax jambu may be used to the identification of the species or in interespecifics ecologics relations

Guarana (Paullinia cupana var. sorbilis), an anciently consumed stimulant from the Amazon rain forest: The seeded-fruit transcriptome

Guarana (Paullinia cupana var. sorbilis) is a plant native to the central Amazon basin. Roasted seed extracts have been used as medicinal beverages since pre-Colombian times, due to their reputation as stimulants, aphrodisiacs, tonics, as well as protectors of the gastrointestinal tract. Guarana plants are commercially cultivated exclusively in Brazil to supply the national carbonated soft-drink industry and natural product stores around the world. In this report, we describe and discuss the annotation of 15,387 ESTs from guarana seeded-fruits, highlighting sequences from the flavonoid and purine alkaloid pathways, and those related to biotic stress avoidance. This is the largest set of sequences registered for the Sapindaceae family. © 2007 Springer-Verlag