Identificação de QTLs ligados a resistência ao crestamento bacteriano em Phaseolus vulgaris.

O objetivo deste trabalho foi identificar QTLs associados à resistência do feijoeiro ao crestamento bacteriano comum, a fim de auxiliar a seleção com marcadores

Fast, simple and cheap: method modified from conventional cultivation for tuberculosis diagnosis allows seeding on Löwenstein-Jensen of any swab-embedded pulmonary samples decontaminated with sodium hydroxide.

Background: Few tuberculosis (TB) control programmes in low-income countries have access to culture facilities in their primary care diagnostic centres and this scenario may have worsened with the coronavirus disease 2019 pandemic. Thus the aim was to develop and evaluate a simpler TB test that allows seeding on Löwenstein?Jensen (LJ) medium of several swab-embedded samples decontaminated with sodium hydroxide (NaOH). Methods: A cotton swab containing each sample was decontaminated in NaOH before being dipped into aslightly acidic solution to neutralize the pH in order to allow the culture to develop on LJ medium. Samples (n=543) from suspected or confirmed pulmonary TB were analysed in two phases: standardization (n=167) and evaluation of the study method (n=376). Results: The study method showed sensitivity >95% and specificity >93% using Ogawa?Kudoh (OK) and modified Petroff (MP) as standards and was comparable to MP-LJ (p>0.05) and slightly superior to OK (p=0.03) for sputum culture and more comprehensive than the latter for other pulmonary specimens. Conclusions: This article reports a more comprehensive, simpler and less costly method for diagnosing TB inthe laboratory with fewer economic resources and biosafety equipment. Thus a patent application was filed (BR1020190103841)

Sistema integração lavoura-pecuária-floresta para a região Semiárida.

O sistema Integração Lavoura Pecuária e Floresta (ILPF) é uma estratégia de cultivo que objetiva a produção sustentável, integrando atividades agrícolas, pecuárias e fl orestais na mesma área, em cultivo consorciado, em sucessão ou rotacionado, e busca efeitos sinérgicos entre os componentes do agroecossistema, contemplando a melhoria ambiental, a valorização dos seres humanos e a viabilidade econômica. Em outras palavras, o sistema ILPF consiste na diversifi cação de cultivo, de forma que o produtor consiga, além de melhorar sua renda, conservar o solo e água da propriedade, melhorando suas condições de cultivo ao longo do tempo, e com melhor aproveitamento de água da chuva. Através deste sistema, o produtor, sendo familiar ou empresarial, tem a possibilidade de produzir grãos, tanto para venda como uso na propriedade, forragem para alimentação animal e madeira. Ainda, o sistema traz outros benefícios, como o aumento da quantidade de matéria orgânica no solo, a cobertura do solo e proteção contra a erosão e o efeito do sombreamento das árvores, que proporciona maior conforto térmico para os animais. Existem várias formas de se fazer cultivo em sistema ILPF, através do uso de diferentes tipos de culturas, forragens e espécies arbóreas, sendo que o ideal para cada caso vai variar em função da região, solo, clima, mão de obra disponível, entre outros, conforme apresentado a seguir, para a região Semiárida

Produtividade da soja inoculada com Bradyrhizobium e Azospirillum em sucessão à braquiária Paiaguás.

bitstream/item/148581/1/37-produtivadade-da-soja-inoculada.pd

The fraction of cancer attributable to ways of life, infections, occupation, and environmental agents in Brazil in 2020

Many human cancers develop as a result of exposure to risk factors related to the environment and ways of life. The aim of this study was to estimate attributable fractions of 25 types of cancers resulting from exposure to modifiable risk factors in Brazil. The prevalence of exposure to selected risk factors among adults was obtained from population-based surveys conducted from 2000 to 2008. Risk estimates were based on data drawn from metaanalyses or large, high quality studies. Population-attributable fractions (PAF) for a combination of risk factors, as well as the number of preventable deaths and cancer cases, were calculated for 2020. The known preventable risk factors studied will account for 34% of cancer cases among men and 35% among women in 2020, and for 46% and 39% deaths, respectively. The highest attributable fractions were estimated for tobacco smoking, infections, low consumption of fruits and vegetables, excess weight, reproductive factors, and physical inactivity. This is the first study to systematically estimate the fraction of cancer attributable to potentially modifiable risk factors in Brazil. Strategies for primary prevention of tobacco smoking and control of infection and the promotion of a healthy diet and physical activity should be the main priorities in policies for cancer prevention in the country. \ua9 2016 Azevedo e Silva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Comparação entre os métodos de Ogawa-Kudoh e Petroff modificado para o cultivo de micobactérias no diagnóstico da tuberculose pulmonar.

RESUMO Objetivo: Comparar o desempenho do método de Ogawa-Kudoh ao de Petroff modificado no diagnóstico da tuberculose pulmonar. Métodos: Utilizaram-se 205 amostras de escarro de 166 pacientes com suspeita clínica ou controle de tuberculose pulmonar atendidos em um hospital público terciário, entre os meses de julho de 2014 a julho de 2016. Todas as amostras foram processadas simultaneamente pelos métodos de descontaminação Ogawa-Kudoh e Petroff modificado, seguindo as recomendações do Ministério da Saúde. Na análise estatística, foi empregado o teste de McNemar, para comparação de proporções, e o índice Kappa, para verificar o grau de concordância entre os dados. Resultados: Os métodos Ogawa-Kudoh e Petroff modificado mostraram-se eficientes na detecção de micobactérias, não sendo verificadas discordâncias significativas tanto nas comparações de pares de resultados (p=0,549), como na taxa de contaminação das culturas (p=0,065). O grau de concordância das técnicas foi considerado excelente (índice Kappa de 0,877), e o Ogawa-Kudoh, em relação ao Petroff modificado, apresentou 90,4% de sensibilidade, 96,6% de especificidade, 94,3% de valor preditivo positivo e 94,2% de valor preditivo negativo. Conclusão: O método de Ogawa-Kudoh revelou-se suficientemente sensível e específico para o diagnóstico da tuberculose pulmonar e, portanto, adequado para a aplicação na rotina laboratorial. Por ser mais simples, de baixo custo e com menores exigências técnicas de biossegurança e capacitação profissional, o Ogawa-Kudoh apresenta-se como alternativa para gestores e profissionais da área promoverem a ampliação da cobertura diagnóstica bacteriológica da tuberculose pulmonar

Deviation from Mendelian transmission of autosomal SNPs can be used to estimate germline mutations in humans exposed to ionizing radiation.

We aimed to estimate the rate of germline mutations in the offspring of individuals accidentally exposed to Cesium-137 ionizing radiation. The study included two distinct groups: one of cases, consisting of males and females accidentally exposed to low doses of ionizing radiation of Cs137, and a control group of non-exposed participants. The cases included 37 people representing 11 families and 15 children conceived after the accident. Exposed families incurred radiation absorbed doses in the range of 0.2 to 0.5 Gray. The control group included 15 families and 15 children also conceived after 1987 in Goiânia with no history of radiation exposure. DNA samples from peripheral blood were analyzed with the Affymetrix GeneChip® CytoScanHD™ to estimate point mutations in autosomal SNPs. A set of scripts previously developed was used to detect de novo mutations by comparing parent and offspring genotypes at the level of each SNP marker. Overall numbers of observed Mendelian deviations were statistically significant between the exposed and control groups. Our retrospective transgenerational DNA analysis showed a 44.0% increase in the burden of SNP mutations in the offspring of cases when compared to controls, based on the average of MFMD for the two groups. Parent-of-origin and type of nucleotide substitution were also inferred. This proved useful in a retrospective estimation of the rate of de novo germline mutations in a human population accidentally exposed to low doses of radiation from Cesium-137. Our results suggested that observed burden of germline mutations identified in offspring was a potentially useful biomarker of effect to estimate parental exposure to low doses of IR and could become an important marker suitable for biomonitoring human population exposed to environmental mutagens

Soil chemical quality in integrated production systems with the presence of native and exotic tree components in the Brazilian Eastern Amazon.

Conservation systems involving trees enhance the sustainability of tropical soils. However, little is known on the effect of integrated systems with native and exotic trees on soil chemical quality in the eastern Amazon

Radiation Campaign of HPK Prototype LGAD sensors for the High-Granularity Timing Detector (HGTD)

We report on the results of a radiation campaign with neutrons and protons of Low Gain Avalanche Detectors (LGAD) produced by Hamamatsu (HPK) as prototypes for the High-Granularity Timing Detector (HGTD) in ATLAS. Sensors with an active thickness of 50~$\mu$m were irradiated in steps of roughly 2$\times$ up to a fluence of $3\times10^{15}~\mathrm{n_{eq}cm^{-2}}$. As a function of the fluence, the collected charge and time resolution of the irradiated sensors will be reported for operation at $-30^{\circ}$