Scanning Electron Microscopic Study of the Surface of Feline Gastric Epithelium: A Simple Method of Removing the Coating Material

Scanning electron microscopic examination of the gastric surface epithelial cells is often hindered by the presence of a coating material. Several methods for removal of coating material on feline gastric mucosa were utilized. The cleansed tissues were evaluated using the scanning electron microscope to assess damage caused by the use of various cleansing methods to surface epithelial cells. The stretched stomach washed several times, including rubbing the mucosal surface with gloved fingers, yielded the best results with no apparent damage to the surface epithelial cells. Flushing unstretched stomachs with saline only did not adequately remove coating material. Flushing unstretched stomachs with saline while stroking the surface with a cotton tipped applicator stick removed debris but damaged the surface epithelium

The Normal Structure of Regional Feline Gastric Mucosae: Scanning Electron Microscopic Study

Regions of cat\u27s stomach can be identified by looking at the surface epithelial cells by scanning electron microscopy (SEM). The luminal surface of cells of the cardiac region were elongated, of the fundus rounded, of the corpus polygonal shaped, and of the pyloric region diamond shaped. The quantity and distribution of microvilli covering the epithelial cells varies, being abundant and evenly distributed in the cardiac region and gradually decreasing in number toward the gastro-duodenal junction, where they were confined to cell perimeters. The colliculi varied in shape and distribution from few in the fundus and corpus to numerous in the pyloric region. Large numbers of gastric pits were present in the corpus. They diminish toward both the cardia and gastro-duodenal junction. The cardiac and pyloric glands were coiled. The gastric glands (glandula gastrica propria) were straight tubules in the fundus and coiled in the corpus. All luminal surfaces of glandular epithelial cells were covered with microvilli, but the regional distribution of microvilli on the cell was variable. Parietal, mucous neck, and chief (zymogen) cells were identified by their cytoplasmic structure. Parietal cells had long apical microvilli, mucous neck cells contained large numbers of globular mucous granules, and chief cells were vacuolated. A few G cells (Endocrinocytus gastrointestinalis) were seen in the cardiac region, large numbers in the pyloric region, and not found in fundus or corpus

Observation on the ultrastructure morphology of HeLa cells treated with ethanol: Statistical analysis.

It is estimated that 5.9% of all human deaths are attributable to alcohol consumption and that the harmful use of ethanol ranks among the top five risk factors for causing disease, disability, and death worldwide. Ethanol is known to disrupt phospholipid packing and promote membrane hemifusion at lipid bilayers. With the exception of mitochondria involved in hormone synthesis, the sterol content of mitochondrial membranes is low. As membranes that are low in cholesterol have increased membrane fluidity and are the most easily disordered by ethanol, we hypothesize that mitochondria are sensitive targets for ethanol damage. HeLa cells were exposed to 50 mM ethanol and the direct effects of ethanol on cellular ultrastructure were examined utilizing transmission electron microscopy. Our ultramicroscopic analysis revealed that cells exposed to ethanol harbor fewer incidence of apoptotic morphology; however, significant alterations to mitochondria and to nuclei occurred. We observed statistical increases in the amount of irregular cells and cells with multiple nuclei, nuclei harboring indentations, and nuclei with multiple nucleolus-like bodies. Indeed, our analysis revealed that mitochondrial damage is the most extensive type of cellular damage. Rupturing of cristae was the most prominent damage followed by mitochondrial swelling. Ethanol exposure also resulted in increased amounts of mitochondrial rupturing, organelles with linked membranes, and mitochondria localizing to indentations of nuclear membranes. We theorize that these alterations could contribute to cellular defects in oxidative phosphorylation and, by extension, the inability to generate regular levels of cellular adenosine triphosphate

PENGARUH KEPEMIMPINAN DAN DISIPLIN KERJA TERHADAP KINERJA KARYAWAN PADA PT. DISTRIBUSI SENTRA JAYA

Tujuan. Penelitian ini bertujuan untuk mengetahui adakah pengaruh atau seberapa besarkah pengaruh variabel kepemimpinan dan disiplin kerja terhadap kinerja karyawan PT. Distribusi Sentra Jaya - Tangerang Selatan.Metode. Metode yang digunakan dalam penelitian ini adalah metode kuantitatif, dimana teknik pengumpulan data yang digunakan dengan penyebaran kuesioner. Sampel dalam penelitian ini adalah karyawan PT. Distribusi Sentra Jaya Tangerang Selatan yang berjumlah 65 orang dengan metode sampel jenuh. Metode yang digunakan adalah regresi linear berganda.Hasil. Dari hasil pengolahan data menggunakan SPSS 26 didapati bahwa Adjusted R Square sebesar 49,2%. Artinya, variabel Kepemimpinan dan Disiplin Kerja dapat menjelaskan variabel Kinerja sebesar 49,2%. Selisihnya, dipengaruhi oleh variabel lain yang tidak digunakan dalam penelitian ini. Hasil dari penelitian ini menyimpulkan bahwa secara parsial, variabel kepemimpinan dan disiplin kerja berpengaruh signifikan terhadap kinerja karyawan PT. Distribusi Sentra Jaya - Tangerang Selatan. Secara simultan, kepemimpinan dan disiplin kerja berpengaruh terhadap Kinerja karyawan.Implikasi. Perusahaan harus menumbuhkan kesadaran pada seluruh karyawan agar memiliki Tanggung jawab menyelesaiakan pekerjaan. Perusahaan harus memberikan tempat kerja yang nyaman agar karyawan bekerja ihklas