Effect of time, moisture content, and substrate amount on sorbitol production using entrapment of Lactobacillus plantarum (BAA-793) in sodium alginate beads

Agro-industrial wastes such as Meranti wood sawdust (MWS) have great potential as substrates for the production of sorbitol and other biochemical products. In this study, treated cellulose from MWS was used as a substrate to produce sorbitol via solid state fermentation (SSF) process using immobilized cells of Lactobacillus plantarum strain (BAA-793), entrapped in sodium alginate. The effect of fermentation time, moisture content, and substrate amount on sorbitol concentration were studied at the following ranges (fermentation time: 2–8 h, moisture content: 40–80%, and substrate amount: 0.5–2.5 g). The results show that the fermentation time of 4 h, substrate amount of 2 g and moisture content of 50% yielded 8.396 g/L of sorbitol. With a moisture content of 50%, substrate amount of 2 g, and fermentation time of 6 h yielded 4.726 g/L of sorbitol. The highest concentration of sorbitol (13.607 g/L) was obtained at the optimized condition of fermentation time 4 h, moisture content 50%, and substrate amount 1.0 g. These results indicate that the fermentation time, moisture content, and substrate amount are important factors to be considered in order to achieve high sorbitol yield

Effect of agitation rate on butanol and ethanol production from palm oil mill effluent by Clostridium acetobutylicum

Malaysia is world largest palm oil producing country. The crude palm oil mill releases liquid effluent known as palm oil mill effluent (POME). These waste result in a significant environmental problem if not dispose in proper manner. The objective of this research is to study the effect of agitation rate on butanol and ethanol production from palm oil mill effluent by Clostridium acetobutylicum. This study also investigated the type of sugar that contain in POME, the profile growth rate of C.acetobutylicum and then to investigate consumption of glucose by C. acetobutylicum during fermentation. The experiment works were conducted in schott bottle in anaerobic chamber to maintain anaerobic condition. Fermentation were carried out for 72 hours at 35oC and maintain the concentration of POME (90%) and inoculums concentration (10%) using palm oil mill effluent and reinforced clostridia medium as a substrate in batch culture. The speed that used in this investigation are, 100, 150, 175 and 200 rpm. The result showed that the yield of butanol and ethanol production will increase as the speed increase until it reaches the optimum point (200 rpm). From this study, it was observed that optimum condition for butanol and ethanol fermentation by C. acetobutylicum is at 200 rpm. The concentration of butanol and ethanol from POME for 200 rpm at optimum time (72 hours) is 0.137 g/l and 68.958 g/l respectively. The result of this experiment showed that fresh POME is a viable media for butanol and ethanol fermentation by C. acetobutylicu

Production of Sorbitol from Meranti Wood Sawdust Using Solid State Fermentation (SSF) Process

The main objective of this research is to produce a high concentrations of sorbitol using solid state fermentation (SSF) of meranti wood dust by bacteria Lactobacillus plantarum (BAA 793; NCIMB 8826). Before the fermentation process, meranti wood dust has been treated through physical and chemical processes for the recovery of cellulose, followed by enzymatic hydrolysis process to produce glucose. The resulting glucose is then used as the carbon sources in fermentation to produce sorbitol. Parameters studied in sorbitol production using solid-state fermentation is fermentation time (2 hours to 14 hours), moisture content (40% to 90%) and temperature (25oC to 45oC). Method one factor at a time (OFAT) conducted on all parameters to determine an appropriate range before the response surface methodology (RSM) is implemented. From this study, the results showed that the optimum condition for the production of sorbitol is at 10 hours, with 50% moisture content and temperature of 35oC where the production of sorbitol is 29.0625g/L. Results of this experiment showed that SSF produce high sorbitol than SMF

Effect of Fermentation Time, Moisture Content, and Temperature on Sorbitol Production Via Solid State Fermentation Process

Malaysia is the largest country that has produced many types of waste. One of it is Meranti wood sawdust. These wastes result in a significant environmental problem if not dispose it in the proper manner. The main objective of this article is to produce the high yield of sorbitol by solid state Fermentation (SSF) process from pretreated Meranti wood sawdust using bacterium Lactobacillus plantarum (BAA 793; NCIMB 8826). One factor at a time (OFAT) was studied for further process using solid state fermentation (SSF) process and investigated the effect of relevant parameters (fermentation time, range: 2 hours to14 hours, moisture content, range: 40% to 90%, temperature, range: 25oC to 45oC) to the solid-state fermentation (SSF) process in producing high yield of sorbitol. The highest product yield was obtained at 50% moisture content, at 10 hours of fermentation time and 35oC of incubation temperature where the concentration of sorbitol was 25.68 g/L respectively. This study also showed that the solid state fermentation (SSF) process will produce the high yield of sorbitol production compared to the submerged fermentation and could serve as a-low cost substrate for bioproducts production especially sorbitol

Characterization of Meranti wood Sawdust and Removal of Lignin Content using Pre-treatment Process

Meranti wood sawdust (MWS) is consisting of major components such as lignin, hemicellulose, and cellulose. The characterizations of MWS are important to determine the composition in MWS.The objective of this study is to characterize MWS and recover cellulose after pretreatment applied with the removal of lignin content. In order to remove the lignin content from MWS, physical pretreatment, and chemical pretreatment was applied. The physical pretreatment involved process such as drying, grinding, screening a sample for the uniform size of the sample less than 0.5mm and autoclaved samples to make sure that, no microorganisms react with the sample (MWS).Then for chemical pretreatment process, type of chemicals that used to remove lignin content in MWS included sodium hydroxide (NaOH), peracetic acid (CH 3 COOH) and acid sulfuric (H 2 SO 4 ).The result shows that, MWS contains - Cellulose: 41.58%, Hemicellulose: 32.81 % Lignin: 33.56 %, Extractives: 3.08 % and Ash: 0.64 %. The result also shows that after using NaOH solution, peracetic acid solution and sulfuric acid solution, the percentage of lignin content in MWS was reduced from 33.56 % to 0.31 % left in MWS. This means that sodium hydroxide (NaOH), peracetic acid (CH3COOH) and acid sulfuric (H2SO4) are categorized as a good agent for treatment. The low lignin content and the high composition of cellulose was important in order to use as energy sources to convert into biochemical products and used as energy sources feedstock for further processing

Production of Sorbitol from Meranti Wood Sawdust (MWS) using Immobilized Cells via Solid State Fermentation (SSF) Process:OFAT Study

Malaysia is the largest country that has produced many types of waste. One of it is Meranti wood sawdust (MWS). These wastes result in a significant environmental problem if not dispose of in the proper manner. The Agroindustrial like MWS has great potential as a substrate for sorbitol fermentation and others biochemical products. The objective of this study is to produce the high production of sorbitol via solid state fermentation (SSF) process from treated MWS using immobilization cells of bacteria (Lactobacillus plantarum sp. strain BAA 793) by entrapment technique (entrapped in sodium alginate). Besides that, one factor at a time (OFAT) were study for further process by solid state fermentation (SSF) process using immobilized cells and investigated the effect of relevant parameters (fermentation time, range: 2hours to 8hours, moisture content, range: 40% to 80%, substrate amount, range: 0.5gram to 2.5gram) to the solid-state fermentation (SSF) process in producing high yield of sorbitol production. The optimum fermentation time is at 4 hours after fixing the substrate amount at 2 g and moisture content at 50 % and the sorbitol production was about 8.396 g/L. Then, 50 % of moisture content will give optimum production of sorbitol, whereby the product was about 4.726 g/L after fixing the substrate amount at 2 g and fermentation time at 6 hours. The production of sorbitol is optimum at 1.0 g of substrate where is the production of sorbitol is about 12.210 g/L after fermentation time was maintained at 50% and 4 hours The highest product was obtained at 50% moisture content, at 4 hours of fermentation time and 1.0 gram of substrate amount whereby the concentrations of sorbitol was 12.21 g/L respectively. These results also indicate that the solid state fermentation (SSF) process will produce the high yield of sorbitol production using immobilized cells with control the important parameter

Optimization Of Sorbitol Production Using Immobilized Of Lactobacillus Plantarum Strain (BAA-793) Via Solid State Fermentation (SSF) Process: Response Surface Methodology (RSM)

Background: The process introduced in this work is the production of sorbitol via solid state fermentation (SSF) using immobilized cells of bacteria. The raw material that was used in this study is Meranti wood sawdust (MWS) that was obtained from Kilang Kayu Aman, Gambang Kuantan, Pahang. The bacterium used was Lactobacillus plantarum (BAA-793) and was immobilized using the entrapment technique (entrapped in sodium alginate). The pretreatment processes used for the treatment of the MWS to recover the cellulose from MWS are the physical pretreatment and chemical pretreatments. The last phase of this works was fermentation using solid state (SSF) process to convert the cellulose to sorbitol. Objective: This study aim to optimize sorbitol production via solid state fermentation (SSF) process using response surface methodology (RSM) and the central composite design (CCD) was used in order to reduce the total number of experiments; besides, to determine the best combination of parameters for optimization of the process. Results: The result shows that the interactions between parameters like moisture content and substrate amount have a very significant effect and the p-value was < 0.0001. The highest yield of sorbitol production (13.607 g/L) was obtained at the condition comprised of 50 % of moisture content, 4 h of fermentation time and 1.0 gram of substrate amount. Conclusion: The production of sorbitol will increase when all the process parameters in the solid state fermentation (SSF) process have been optimized. The RSM was also suitable for fitting a quadratic surface and it also helped to optimize the effective parameters with a minimum number of experiments as well as to analyze the interaction between the parameters