Examination Free-Flow Speed Distribution on Two-Lane Rural Roads

[EN] Free-flow speed variation of passenger vehicles along a road segment is one of the most used factors in road safety studies, as a surrogate measure to evaluate road design consistency. Free-flow speed may be measured when a road segment is already built but must be estimated during the design phase. Several studies have been carried out to calibrate models to estimate free-flow speed, with geometric features as explanatory variables. Currently, most free-flow speed models focus only on mean speed or speed in particular percentiles, such as the 85th or 95th. Moreover, most studies have assumed normality in the free-flow speed distribution without checking this hypothesis. The main objective of this study was to analyze the free-flow speed distribution on two-lane rural road curves and tangents. The research focused on two main issues: determining whether speed data were normally distributed at a specific site and analyzing the behavior of the mean and standard deviation of speed on curves and tangents. The study was based on continuous operating speed profiles, which were obtained from a database of more than 16,000 vehicles/km. A total of 63 horizontal curves and 78 tangents were analyzed. According to the results, the normal distribution is not the best distribution in most cases for describing free-flow speeds. In 46 of the curves and 64 of the tangents, free-flow speed cannot be assumed to be normally distributed. Therefore, some other distributions should be tested in further research.The study presented in this paper is part of the research project CASEFU-Estudio experimental de la funcionalidad y seguridad de las carreteras convencionales, subsidized by the Spanish Ministry of Economy and Competitiveness and the European Social Fund. In addition, the authors thank the Center for Studies and Experimentation of Public Works of the Spanish Ministry of Public Works for subsidizing the field data collection, and the Infrastructure and Transportation Department of the General Directorate of Public Works of the Valencian Government, the Valencian Provincial Council, and the Ministry of the Interior, especially the General Directorate of Traffic of Spain, for their cooperation in gathering the field data.García Jiménez, ME.; Pérez Zuriaga, AM.; Llopis-Castelló, D.; Camacho Torregrosa, FJ.; García García, A. (2016). Examination Free-Flow Speed Distribution on Two-Lane Rural Roads. Transportation Research Record Journal of the Transportation Research Board. 2556:86-97. https://doi.org/10.3141/2556-09S8697255

Validation of Low-Cost Driving Simulator Based on Continuous Speed Profiles

[EN] The number of road safety studies that are based on driving simulators is growing significantly. The Polytechnic University of Valencia, Spain, developed a low-cost driving simulator for the assessment, training, and rehabilitation of drivers (SE2RCO). The main objective of this research was the validation of the driving simulator so that studies about road safety and highway geometric design that considered human factors could be performed. The validation was based on continuous speed profiles collected from 28 volunteers on a 30-km-long, two-lane rural road section. The same volunteers drove through the same road section built in SE2RCO. Speed data of 79 curves and 52 tangents were selected for the analysis. Comparison of the real and simulated speeds ensured the simulator's objective validity according to average and operating speeds. Two models were developed to predict field speeds from simulated speeds. Results showed that a simulated average speed lower than approximately 90 km/h was linked to a similar real average speed. For higher simulated speeds, the average speed in the real environment was lower than the simulated one. In addition, the actual operating speed was around 5 km/h lower than the operating speed in the driving simulator. Most volunteers assessed the quality and similarity of the virtual environment compared with the real world as medium or high and assessed the driving tasks similarly, thus achieving subjective validation of the simulator.The authors thank the Polytechnic University of Valencia, which subsidized the research project CONSIM-Desarrollo de un Modelo para la Evaluacion de la Consistencia del Diseno Geometric de Carreteras Convencionales Mediante Simuladores de Conduccion. The study presented here was also part of the research project titled CASEFU-Estudio Experimental de la Funcionalidad y Seguridad de las Carreteras Convencionales, which was subsidized by the Spanish Ministry of Economy and Competitiveness and the European Social Fund.Llopis-Castelló, D.; Camacho Torregrosa, FJ.; Marín-Morales, J.; Pérez Zuriaga, AM.; García García, A.; Dols Ruiz, JF. (2016). Validation of Low-Cost Driving Simulator Based on Continuous Speed Profiles. Transportation Research Record Journal of the Transportation Research Board. 2602:104-114. https://doi.org/10.3141/2602-13S104114260

PIME Aprendizaje Basado en Proyectos: Ingeniería de Carreteras e Ingeniería Geotécnica.

[ES] En los últimos años, la Universitat Politècnica de València (UPV) ha promovido, dentro de su programa de innovación educativa “Aprendizaje y Docencia” (A+D), la puesta en marcha de Proyectos de Innovación y Mejora Educativa (PIME). Durante el curso 2019/2020 se ha implantado un PIME basado en Aprendizaje Basado en Proyectos (ABP) que reúne a dos asignaturas consecutivas de tercer curso de Ingeniería Civil. En este artículo se muestran los resultados alcanzados en el primer semestre de implantación. Los alumnos han expresado claramente su interés y preferencia por este tipo de metodología. Han demostrado haber adquirido un aprendizaje en profundidad y ser capaces de adquirir conocimientos de forma autónoma, así como transferir estos a la realidad de la profesión mediante el desarrollo de su capacidad de trabajar en equipo y la posibilidad de realizar visitas de campo donde los conocimientos recibidos adquieren un carácter práctico. Además, han desarrollado las competencias esperadas, integrando aspectos académicos con otros sociales y éticos.[EN] In recent years, the Polytechnic University of Valencia has encouraged the implementation of Educational Innovation and Improvement Projects (PIME) within the "Learning and Teaching" (A+D) program. During the 2019/2020 academic year, a PIME based on Problem or Project Based Learning (PBL) was implemented, bringing together two consecutive subjects from the third year of Civil Engineering. This article shows the results achieved in the first semester after implantation. The students have clearly expressed their interest and preference for this kind of methodology. They have obtained an in-depth learning and they are prepared to acquire knowledge by themselves, as well as transferring it to professional practice by developing their ability to work as a team and the possibility of visit the studio area, so the knowledges acquire a practical character. They have also developed the expected skills , integrating academic aspects with other social and ethical oneGarrido De La Torre, ME.; Pérez-Zuriaga, AM.; Martínez-Ibáñez, V.; López Maldonado, G.; Cuadrado Tarodo, Á. (2020). PIME Aprendizaje Basado en Proyectos: Ingeniería de Carreteras e Ingeniería Geotécnica. En IN-RED 2020: VI Congreso de Innovación Educativa y Docencia en Red. Editorial Universitat Politècnica de València. 293-306. https://doi.org/10.4995/INRED2020.2020.11929OCS29330

Estado fitosanitario del azafrán en Aragón (España): Insectos, ácaros, nematodos, virus, bacterias y malas hierbas

El azafrán cultivado en España está adquiriendo relevancia en las últimas décadas como producto de gran calidad, lo que requiere la selección de cormos, sus órganos reproductivos, sanos para la plantación con el objetivo de mantener un adecuado estado fitosanitario del cultivo. Este trabajo presenta un estudio del estado fitosanitario del azafrán en Teruel, donde el cultivo fue muy importante económica y socialmente. Además, este estudio pretende ser de utilidad para las zonas productoras con características agroclimáticas similares. Con dichos objetivos, se prospectaron 10 plantaciones comerciales de azafrán en 6 localidades del valle del Jiloca entre los años 2008 y 2011, estudiando la presencia de insectos, ácaros, nematodos, virus, bacterias y malas hierbas. El ácaro Rhizoglyphus robini, una de las plagas más importantes del azafrán, se detectó en los cormos y en el suelo en una parcela. También el nematodo Aphelenchoides blastophtorus, plaga en plantas ornamentales, se encontró abundantemente en cormos de dos parcelas. Se detectaron infecciones ocasionales de virus del género potyvirus en el cultivo y en la mala hierba Eruca vesicaria. Las malas hierbas Lolium rigidum y Descurainia sophia podrían causar serios problemas de competencia al cultivo y se considera necesario realizar operaciones de escarda en otoño y en invierno. No se detectaron insectos nocivos ni bacterias fitopatógenas. La multiplicación vegetativa del azafrán hace aconsejable realizar muestreos, especialmente en los cormos antes de ser replantados, para detectar la presencia de ácaros, nematodos y virus que podrían ocasionar pérdidas de producción y calidad. In the last decades, saffron produced in Spain is gaining relevance as a high-quality product, which requires the selection of healthy corms (the reproductive organ) for planting in order to maintain adequa-te phytosanitary status of the crop. In this work, the phytosanitary status of saffron was studied in Teruel (Aragón, Spain), where the crop has economic and social importance. Moreover, it aims to be useful for the production areas with similar agro-climatic characteristics. Ten commercial saffron plantations in six locations of the Jiloca valley have been surveyed between 2008 and 2011 and the presence of insects, mites, nematodes, virus, bacteria and weeds was studied. The mite Rhizoglyphus robini, one of the most important pests of saffron, was detected in both corms and soil in one plantation. The nematode Aphelenchoides blastophtorus, pest in ornamental plants, was also found in corms in two plantations. Potyvirus infections were occasionally detected in both the crop and in the weed Eruca vesicaria. The weeds Lolium rigidum and Descurainia sophia could cause diminutions of the yield by competition, therefore, weeding operations are necessary in autumn and winter. No harmful insects and phytopatogenic bacteria were detected. Because reproduction is only possible through corm propagation, it is advisable to analyse the plants, especially the corms, before being re-planted, in order to detect the presence of mites, nematodes and virus that could reduce yield and quality decreases

WNT5A-JNK regulation of vascular insulin resistance in human obesity

Obesity is associated with the development of vascular insulin resistance; however, pathophysiological mechanisms are poorly understood. We sought to investigate the role of WNT5A-JNK in the regulation of insulin-mediated vasodilator responses in human adipose tissue arterioles prone to endothelial dysfunction. In 43 severely obese (BMI 44±11 kg/m2) and five metabolically normal non-obese (BMI 26±2 kg/m2) subjects, we isolated arterioles from subcutaneous and visceral fat during planned surgeries. Using videomicroscopy, we examined insulin-mediated, endothelium-dependent vasodilator responses and characterized adipose tissue gene and protein expression using real-time polymerase chain reaction and Western blot analyses. Immunofluorescence was used to quantify endothelial nitric oxide synthase (eNOS) phosphorylation. Insulin-mediated vasodilation was markedly impaired in visceral compared to subcutaneous vessels from obese subjects (pWNT5A and its non-canonical receptors, which correlated negatively with insulin signaling. Pharmacological JNK antagonism with SP600125 markedly improved insulin-mediated vasodilation by sixfold (p

Under pressure: phenotypic divergence and convergence associated with microhabitat adaptations in Triatominae.

BACKGROUND: Triatomine bugs, the vectors of Chagas disease, associate with vertebrate hosts in highly diverse ecotopes. It has been proposed that occupation of new microhabitats may trigger selection for distinct phenotypic variants in these blood-sucking bugs. Although understanding phenotypic variation is key to the study of adaptive evolution and central to phenotype-based taxonomy, the drivers of phenotypic change and diversity in triatomines remain poorly understood. METHODS/RESULTS: We combined a detailed phenotypic appraisal (including morphology and morphometrics) with mitochondrial cytb and nuclear ITS2 DNA sequence analyses to study Rhodnius ecuadoriensis populations from across the species' range. We found three major, naked-eye phenotypic variants. Southern-Andean bugs primarily from vertebrate-nest microhabitats (Ecuador/Peru) are typical, light-colored, small bugs with short heads/wings. Northern-Andean bugs from wet-forest palms (Ecuador) are dark, large bugs with long heads/wings. Finally, northern-lowland bugs primarily from dry-forest palms (Ecuador) are light-colored and medium-sized. Wing and (size-free) head shapes are similar across Ecuadorian populations, regardless of habitat or phenotype, but distinct in Peruvian bugs. Bayesian phylogenetic and multispecies-coalescent DNA sequence analyses strongly suggest that Ecuadorian and Peruvian populations are two independently evolving lineages, with little within-lineage phylogeographic structuring or differentiation. CONCLUSIONS: We report sharp naked-eye phenotypic divergence of genetically similar Ecuadorian R. ecuadoriensis (nest-dwelling southern-Andean vs palm-dwelling northern bugs; and palm-dwelling Andean vs lowland), and sharp naked-eye phenotypic similarity of typical, yet genetically distinct, southern-Andean bugs primarily from vertebrate-nest (but not palm) microhabitats. This remarkable phenotypic diversity within a single nominal species likely stems from microhabitat adaptations possibly involving predator-driven selection (yielding substrate-matching camouflage coloration) and a shift from palm-crown to vertebrate-nest microhabitats (yielding smaller bodies and shorter and stouter heads). These findings shed new light on the origins of phenotypic diversity in triatomines, warn against excess reliance on phenotype-based triatomine-bug taxonomy, and confirm the Triatominae as an informative model system for the study of phenotypic change under ecological pressure

Correction to: Under pressure: phenotypic divergence and convergence associated with microhabitat adaptations in Triatominae.

An amendment to this paper has been published and can be accessed via the original article

Psychometric Properties and factor structure of the spanish version of the HC-PAIRS questionnaire

Objective To develop a Spanish version of the Health Care Providers" Pain and Impairment Relationship Scale (HC-PAIRS) and to test its psychometric properties. Methods A forward and backward translation methodology was used to translate the questionnaire, which was then applied to 206 participants (174physiotherapy students and 32 family physicians). The intraclass correlation coefficient was calculated to assess testretest reliability. Internal consistency was evaluated using Cronbach"s alpha and item analysis. Construct validity was measured using Pearson correlation coefficients between HC-PAIRS and FABQ, FABQ-Phys, FABQ-Work and the responses given by participants to three clinical case scenarios. An exploratory factor analysis was carried out following the Kaiser normalization criteria and principal axis factoring with an oblique rotation (quartimax). Sensitivity to change was assessed after a teaching module. Results Testretest reliability was ICC 0.50 (p\0.01)and Cronbach"s alpha was 0.825. The HC-PAIRS scores correlated significantly with the scores of the FABQ and also with the recommendations for work and activity given by the participants in the three clinical case scenarios. Sensitivity to change test showed an effect size of 1.5, which is considered a large change. Factor analysis suggests that the Spanish version of HC-PAIRS measures a unidimensional construct. Conclusion The Spanish version of the HC-PAIRS has proven to be a reliable, valid and sensitive instrument to assess health care providers" attitudes and beliefs about LBP. It can be used in evaluating clinical practice and in undergraduate acquisition of skills and knowledge

Genomic variation in tomato, from wild ancestors to contemporary breeding accessions

[EN] Background: Domestication modifies the genomic variation of species. Quantifying this variation provides insights into the domestication process, facilitates the management of resources used by breeders and germplasm centers, and enables the design of experiments to associate traits with genes. We described and analyzed the genetic diversity of 1,008 tomato accessions including Solanum lycopersicum var. lycopersicum (SLL), S. lycopersicum var. cerasiforme (SLC), and S. pimpinellifolium (SP) that were genotyped using 7,720 SNPs. Additionally, we explored the allelic frequency of six loci affecting fruit weight and shape to infer patterns of selection. Results: Our results revealed a pattern of variation that strongly supported a two-step domestication process, occasional hybridization in the wild, and differentiation through human selection. These interpretations were consistent with the observed allele frequencies for the six loci affecting fruit weight and shape. Fruit weight was strongly selected in SLC in the Andean region of Ecuador and Northern Peru prior to the domestication of tomato in Mesoamerica. Alleles affecting fruit shape were differentially selected among SLL genetic subgroups. Our results also clarified the biological status of SLC. True SLC was phylogenetically positioned between SP and SLL and its fruit morphology was diverse. SLC and “cherry tomato” are not synonymous terms. The morphologically-based term “cherry tomato” included some SLC, contemporary varieties, as well as many admixtures between SP and SLL. Contemporary SLL showed a moderate increase in nucleotide diversity, when compared with vintage groups. Conclusions: This study presents a broad and detailed representation of the genomic variation in tomato. Tomato domestication seems to have followed a two step-process; a first domestication in South America and a second step in Mesoamerica. The distribution of fruit weight and shape alleles supports that domestication of SLC occurred in the Andean region. Our results also clarify the biological status of SLC as true phylogenetic group within tomato. We detect Ecuadorian and Peruvian accessions that may represent a pool of unexplored variation that could be of interest for crop improvement.We are grateful to the gene banks for their collections that made this study possible. We thank Syngenta Seeds for providing genotyping data for 42 accessions. We would like to thank the Supercomputing and Bioinnovation Center (Universidad de Malaga, Spain) for providing computational resources to process the SNAPP phylogenetic tree. This research was supported in part by the USDA/NIFA funded SolCAP project under contract number to DF and USDA AFRI 2013-67013-21229 to EvdK and DF.Blanca Postigo, JM.; Montero Pau, J.; Sauvage, C.; Bauchet, G.; Illa, E.; Díez Niclós, MJTDJ.; Francis, D.... (2015). Genomic variation in tomato, from wild ancestors to contemporary breeding accessions. BMC Genomics. 16(257):1-19. https://doi.org/10.1186/s12864-015-1444-1S11916257Tanksley SD, McCouch SR. Seed banks and molecular maps: unlocking genetic potential from the wild. Science (80-). 1997;277:1063–6.Doebley JF, Gaut BS, Smith BD. The molecular genetics of crop domestication. Cell. 2006;127:1309–21.Gepts P. A comparison between crop domestication, classical plant breeding, and genetic engineering. Crop Sci. 2002;42:1780.Weigel D, Nordborg M. Natural variation in Arabidopsis. How do we find the causal genes? Plant Physiol. 2005;138:567–8.Peralta IE, Spooner DM, Knapp S, Anderson C. Taxonomy of wild tomatoes and their relatives (Solanum sect. Lycopersicoides, sect. Juglandifolia, sect. Lycopersicon; Solanaceae). Syst Bot Monogr. 2008;84:1–186.Rick CM, Fobes JF. Allozyme variation in the cultivated tomato and closely related species. Bull Torrey Bot Club. 1975;102:376–84.Zuriaga E, Blanca J, Nuez F. Classification and phylogenetic relationships in Solanum section Lycopersicon based on AFLP and two nuclear gene sequences. Genet Resour Crop Evol. 2008;56:663–78.Zuriaga E, Blanca J, Cordero L, Sifres A, Blas-Cerdán WG, Morales R, et al. Genetic and bioclimatic variation in Solanum pimpinellifolium. Genet Resour Crop Evol. 2008;56:39–51.Blanca J, Cañizares J, Cordero L, Pascual L, Diez MJ, Nuez F. Variation revealed by SNP genotyping and morphology provides insight into the origin of the tomato. PLoS One. 2012;7:e48198.Rick CM. Natural variability in wild species of Lycopersicon and its bearing on tomato breeding. Genet Agrar. 1976;30:249–59.Rick CM, Holle M. Andean Lycopersicon esculentum var. cerasiforme: genetic variation and its evolutionary significance. Econ Bot. 1990;44:69–78.Nakazato T, Franklin RA, Kirk BC, Housworth EA. Population structure, demographic history, and evolutionary patterns of a green-fruited tomato, Solanum peruvianum (Solanaceae), revealed by spatial genetics analyses. Am J Bot. 2012;99:1207–16.Rick CM, Butler L. Cytogenetics of the Tomato. Adv Genet. 1956;8:267–382. Advances in Genetics.Jenkins JA. The origin of the cultivated tomato. Econ Bot. 1948;2:379–92.Nesbitt TC, Tanksley SD. Comparative sequencing in the genus lycopersicon: implications for the evolution of fruit size in the domestication of cultivated tomatoes. Genetics. 2002;162:365–79.Ranc N, Muños S, Santoni S, Causse M. A clarified position for Solanum lycopersicum var cerasiforme in the evolutionary history of tomatoes (solanaceae). BMC Plant Biol. 2008;8:130.De Candolle A. Origin of cultivated plants. 2nd ed. London: Trench, Paul; 1886.Miller JC, Tanksley SD. RFLP analysis of phylogenetic relationships and genetic variation in the genus Lycopersicon. Theor Appl Genet. 1990;80:437–48.Williams CE, Clair DAS. Phenetic relationships and levels of variability detected by restriction fragment length polymorphism and random amplified polymorphic DNA analysis of cultivated and wild accessions of Lycopersicon esculentum. Genome. 1993;36:619–30.Park YH, West MAL, St Clair DA. Evaluation of AFLPs for germplasm fingerprinting and assessment of genetic diversity in cultivars of tomato (Lycopersicon esculentum L). Genome. 2004;47:510–8.Sim S-C, Robbins MD, Van Deynze A, Michel AP, Francis DM. Population structure and genetic differentiation associated with breeding history and selection in tomato (Solanum lycopersicum L.). Heredity (Edinb). 2011;106:927–35.Sim S-C, Robbins MD, Chilcott C, Zhu T, Francis DM. Oligonucleotide array discovery of polymorphisms in cultivated tomato (Solanum lycopersicum L) reveals patterns of SNP variation associated with breeding. BMC Genomics. 2009;10:466.Sim S-C, Durstewitz G, Plieske J, Wieseke R, Ganal MW, Van Deynze A, et al. Development of a large SNP genotyping array and generation of high-density genetic maps in tomato. PLoS One. 2012;7:e40563.Frary A, Nesbitt TC, Grandillo S, Knaap E, Cong B, Liu J, et al. fw2.2: a quantitative trait locus key to the evolution of tomato fruit size. Science. 2000;289:85–8.Liu J, Van Eck J, Cong B, Tanksley SD. A new class of regulatory genes underlying the cause of pear-shaped tomato fruit. Proc Natl Acad Sci U S A. 2002;99:13302–6.Xiao H, Jiang N, Schaffner E, Stockinger EJ, van der Knaap E. A retrotransposon-mediated gene duplication underlies morphological variation of tomato fruit. Science. 2008;319:1527–30.Cong B, Barrero LS, Tanksley SD. Regulatory change in YABBY-like transcription factor led to evolution of extreme fruit size during tomato domestication. Nat Genet. 2008;40:800–4.Muños S, Ranc N, Botton E, Bérard A, Rolland S, Duffé P, et al. Increase in tomato locule number is controlled by two single-nucleotide polymorphisms located near WUSCHEL. Plant Physiol. 2011;156:2244–54.Chakrabarti M, Zhang N, Sauvage C, Muños S, Blanca J, Cañizares J, et al. A cytochrome P450 regulates a domestication trait in cultivated tomato. Proc Natl Acad Sci U S A. 2013;110:17125–30.Rodríguez GR, Muños S, Anderson C, Sim S-C, Michel A, Causse M, et al. Distribution of SUN, OVATE, LC, and FAS in the tomato germplasm and the relationship to fruit shape diversity. Plant Physiol. 2011;156:275–85.Sim S-C, Van Deynze A, Stoffel K, Douches DS, Zarka D, Ganal MW, et al. High-density SNP genotyping of tomato (Solanum lycopersicum L) reveals patterns of genetic variation due to breeding. PLoS One. 2012;7:e45520.Sauvage C, Segura V, Bauchet G, Stevens R, Thi Do P, Nikoloski Z, et al. Genome Wide Association in tomato reveals 44 candidate loci for fruit metabolic traits. Plant Physiol. 2014;165:1120–32.Hamilton JP, Sim S-C, Stoffel K, Van Deynze A, Buell CR, Francis DM. Single nucleotide polymorphism discovery in cultivated tomato via sequencing by synthesis. Plant Genome J. 2012;5:17.Patterson NJ, Price AL, Reich D. Population structure and eigenanalysis. PLoS Genet. 2006;2:e190.Price AL, Patterson NJ, Plenge RM, Weinblatt ME, Shadick NA, Reich D. Principal components analysis corrects for stratification in genome-wide association studies. Nat Genet. 2006;38:904–9.Kosman E, Leonard KJ. Similarity coefficients for molecular markers in studies of genetic relationships between individuals for haploid, diploid, and polyploid species. Mol Ecol. 2005;14:415–24.Adler D. vioplot: Violin plot. 2005.Jost L. Gst and its relatives do not measure differentiation. Mol Ecol. 2008;17:4015–26.Excoffier L, Lischer H. Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour. 2010;10:564–7.Huson DH, Bryant D. Application of phylogenetic networks in evolutionary studies. Mol Ecol Evol. 2006;23:254–67.Knight R, Maxwell P, Birmingham A, Carnes J, Caporaso JG, Easton BC, et al. PyCogent: a toolkit for making sense from sequence. Genome Biol. 2007;8:R171.Szpiech ZA, Jakobsson M, Rosenberg NA. ADZE: a rarefaction approach for counting alleles private to combinations of populations. Bioinformatics. 2008;24:2498–504.Bradbury PJ, Zhang Z, Kroon DE, Casstevens TM, Ramdoss Y, Buckler ES. TASSEL: software for association mapping of complex traits in diverse samples. Bioinformatics. 2007;23:2633–5.Cleveland WS. Robust locally weighted regression and smoothing scatterplots. J Am Stat Assoc. 1979;74:829.R Core Team. R: A Language and Environment for Statistical Computing. 2013.Sinnot RS. Virtues of the haversine. Sky Telesc. 1984;68:159.Hijmans RJ, Etten JV. raster: Geographic data analysis and Modeling. 2013.Bryant D, Bouckaert R, Felsenstein J, Rosenberg NA, RoyChoudhury A. Inferring species trees directly from biallelic genetic markers: bypassing gene trees in a full coalescent analysis. Mol Biol Evol. 2012;29:1917–32.Drummond AJ, Rambaut A. BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol Biol. 2007;7:214.Rambaut A. Tracer v.1.5. 2009.Huang Z, van der Knaap E. Tomato fruit weight 11.3 maps close to fasciated on the bottom of chromosome 11. Theor Appl Genet. 2011;123:465–74.Guo M, Rupe MA, Dieter JA, Zou J, Spielbauer D, Duncan KE, et al. Cell Number Regulator1 affects plant and organ size in maize: implications for crop yield enhancement and heterosis. Plant Cell. 2010;22:1057–73.Sambrook J, Fritsch EF, Maniatis T. Molecular cloning. New York: Cold Spring Harbor Laboratory Press; 1989.Lin T, Zhu G, Zhang J, Xu X, Yu Q, Zheng Z, et al. Genomic analyses provide insights into the history of tomato breeding. Nat Genet. 2014;46:1220–6.Platt A, Horton M, Huang YS, Li Y, Anastasio AE, Mulyati NW, et al. The scale of population structure in Arabidopsis thaliana. PLoS Genet. 2010;6:e1000843.Pressoir G, Berthaud J. Patterns of population structure in maize landraces from the Central Valleys of Oaxaca in Mexico. Heredity (Edinb). 2004;92:88–94.Koenig D, Jiménez-Gómez JM, Kimura S, Fulop D, Chitwood DH, Headland LR, et al. Comparative transcriptomics reveals patterns of selection in domesticated and wild tomato. Proc Natl Acad Sci U S A. 2013;110:e2655–62.Nakazato T, Housworth EA. Spatial genetics of wild tomato species reveals roles of the Andean geography on demographic history. Am J Bot. 2011;98:88–98.United States. Office of Experimental Stations. Experimental Station Recod, Volumen 39. Volume 39. Washington, DC, USA: United States. Office of Experimental Stations; 1918.Merk HL, Yames SC, Van Deynze A, Tong N, Menda N, Mueller LA, et al. Trait diversity and potential for selection indeces based on variation among regionally adapted processing tomato germplasm. J Am Soc Hortic Sci. 2012;137:427–37

Convalescent plasma in patients admitted to hospital with COVID-19 (RECOVERY): a randomised controlled, open-label, platform trial

Background: Many patients with COVID-19 have been treated with plasma containing anti-SARS-CoV-2 antibodies. We aimed to evaluate the safety and efficacy of convalescent plasma therapy in patients admitted to hospital with COVID-19. Methods: This randomised, controlled, open-label, platform trial (Randomised Evaluation of COVID-19 Therapy [RECOVERY]) is assessing several possible treatments in patients hospitalised with COVID-19 in the UK. The trial is underway at 177 NHS hospitals from across the UK. Eligible and consenting patients were randomly assigned (1:1) to receive either usual care alone (usual care group) or usual care plus high-titre convalescent plasma (convalescent plasma group). The primary outcome was 28-day mortality, analysed on an intention-to-treat basis. The trial is registered with ISRCTN, 50189673, and ClinicalTrials.gov, NCT04381936. Findings: Between May 28, 2020, and Jan 15, 2021, 11558 (71%) of 16287 patients enrolled in RECOVERY were eligible to receive convalescent plasma and were assigned to either the convalescent plasma group or the usual care group. There was no significant difference in 28-day mortality between the two groups: 1399 (24%) of 5795 patients in the convalescent plasma group and 1408 (24%) of 5763 patients in the usual care group died within 28 days (rate ratio 1·00, 95% CI 0·93–1·07; p=0·95). The 28-day mortality rate ratio was similar in all prespecified subgroups of patients, including in those patients without detectable SARS-CoV-2 antibodies at randomisation. Allocation to convalescent plasma had no significant effect on the proportion of patients discharged from hospital within 28 days (3832 [66%] patients in the convalescent plasma group vs 3822 [66%] patients in the usual care group; rate ratio 0·99, 95% CI 0·94–1·03; p=0·57). Among those not on invasive mechanical ventilation at randomisation, there was no significant difference in the proportion of patients meeting the composite endpoint of progression to invasive mechanical ventilation or death (1568 [29%] of 5493 patients in the convalescent plasma group vs 1568 [29%] of 5448 patients in the usual care group; rate ratio 0·99, 95% CI 0·93–1·05; p=0·79). Interpretation: In patients hospitalised with COVID-19, high-titre convalescent plasma did not improve survival or other prespecified clinical outcomes. Funding: UK Research and Innovation (Medical Research Council) and National Institute of Health Research