76 research outputs found
Ultralong coherence times in the purely electronic zero-phonon line emission of single molecules
We report the observation of ultralong coherence times in the purely
electronic zero-phonon line emission of single terrylenediimide molecules at
1.4 K. Vibronic excitation and spectrally resolved detection with a scanning
Fabry-Perot spectrum analyzer were used to measure a linewidth of 65 MHz. This
is within a factor of 1.6 of the transform limit. It therefore indicates that
single molecule emission may be suited for applications in linear optics
quantum computation. Additionally it is shown that high resolution spectra
taken with the spectrum analyzer allow for the investigation of fast spectral
dynamics in the emission of a single molecule.Comment: to appear in Applied Physics Letter
Observation of liquid glass in suspensions of ellipsoidal colloids
Despite the omnipresence of colloidal suspensions, little is known about the
influence of shape on phase transformations, especially in nonequilibrium. To
date, real-space imaging results are limited to systems composed of spherical
colloids. In most natural and technical systems, however, particles are
non-spherical and their structural dynamics are determined by translational and
rotational degrees of freedom. Using confocal microscopy, we reveal that
suspensions of ellipsoidal colloids form an unexpected state of matter, a
liquid glass in which rotations are frozen while translations remain fluid.
Image analysis unveils hitherto unknown nematic precursors as characteristic
structural elements of this state. The mutual obstruction of these ramified
clusters prevents liquid crystalline order. Our results give unique insight
into the interplay between local structures and phase transformations. This
helps to guide applications such as self-assembly of colloidal superstructures
and also gives first evidence of the importance of shape on the glass
transition in general.Comment: 19 pages, 6 figures; additional SI; submitte
SECARS imaging of thiophenol adsorbed on silver colloids
The very large enhancement of the Raman signal observed in SERS (Surface-enhanced Raman Spectroscopy)[1] enabling single molecule sensitivity in some cases, makes it very appealing to apply this same experimental approach to non-linear techniques like CARS spectroscopy as higher enhancement factors are expected due to the quadratic dependence of the signal with the pump laser intensity. In spite of this and though several groups have tried the SECARS (Surface-enhanced Coherent Anti-Stokes Raman Spectroscopy) with different success [2], there is not yet a definitive proof-of-principle of this experimental approach. To this latter gaol has been devoted this work. We have recorded the forward CARS images of thiophenol adsorbed on silver nanoaggregates through the 8a ring stretching mode resonance at 1574 cm-1.Universidad de Málaga. Campus de Excelencia Internacional AndalucĂa Tech
Noninvasive Photoacoustic and Fluorescence Sentinel Lymph Node Identification using Dye-Loaded Perfluorocarbon Nanoparticles
The contrast mechanisms used for photoacoustic tomography (PAT) and fluorescence imaging differ in subtle, but significant, ways. The design of contrast agents for each or both modalities requires an understanding of the spectral characteristics as well as intra- and intermolecular interactions that occur during formulation. We found that fluorescence quenching that occurs in the formulation of near-infrared (NIR) fluorescent dyes in nanoparticles results in enhanced contrast for PAT. The ability of the new PAT method to utilize strongly absorbing chromophores for signal generation allowed us to convert a highly fluorescent dye into an exceptionally high PA contrast material. Spectroscopic characterization of the developed NIR dye-loaded perfluorocarbon-based nanoparticles for combined fluorescence and PA imaging revealed distinct dye-dependent photophysical behavior. We demonstrate that the enhanced contrast allows detection of regional lymph nodes of rats in vivo with time-domain optical and photoacoustic imaging methods. The results further show that the use of fluorescence lifetime imaging, which is less dependent on fluorescence intensity, provides a strategic approach to bridge the disparate contrast reporting mechanisms of fluorescence and PA imaging methods
Noninvasive Photoacoustic and Fluorescence Sentinel Lymph Node Identification using Dye-Loaded Perfluorocarbon Nanoparticles
The contrast mechanisms used for photoacoustic tomography (PAT) and fluorescence imaging differ in subtle, but significant, ways. The design of contrast agents for each or both modalities requires an understanding of the spectral characteristics as well as intra- and intermolecular interactions that occur during formulation. We found that fluorescence quenching that occurs in the formulation of near-infrared (NIR) fluorescent dyes in nanoparticles results in enhanced contrast for PAT. The ability of the new PAT method to utilize strongly absorbing chromophores for signal generation allowed us to convert a highly fluorescent dye into an exceptionally high PA contrast material. Spectroscopic characterization of the developed NIR dye-loaded perfluorocarbon-based nanoparticles for combined fluorescence and PA imaging revealed distinct dye-dependent photophysical behavior. We demonstrate that the enhanced contrast allows detection of regional lymph nodes of rats in vivo with time-domain optical and photoacoustic imaging methods. The results further show that the use of fluorescence lifetime imaging, which is less dependent on fluorescence intensity, provides a strategic approach to bridge the disparate contrast reporting mechanisms of fluorescence and PA imaging methods
Multiphoton microscopy and ultrafast spectroscopy: Imaging meets quantum (MUSIQ) roadmap
In April 2019 the EU Marie Skłodowska-Curie Actions (MSCA) Innovative Training
Networks (ITN) MUSIQ officially started. The network brought together a unique
team of world-leading academics and industrial partners at the forefront of optical
micro-spectroscopy and ultrafast laser technology developments merged with
fundamental studies of coherent light-matter interaction phenomena, development
of quantitative image analysis tools beyond state-of-the-art, and
biomedical/pharmaceutical real-world applications. The unique vision of MUSIQ has
been to develop and apply the next-generation optical microscopy technologies
exploiting quantum coherent nonlinear phenomena. This Roadmap has been written
collectively by the MUSIQ early-stage researchers and their supervisors. It provides a
summary of the achievements within MUSIQ to date, with an outlook towards future
directions
Total-internal-reflection fluorescence microscopy with W-shaped axicon mirrors
A scheme based on a W-shaped axicon mirror device for total-internal-reflection fluorescence microscopy (TIRFM)is presented. This approach combines the advantages of higher efficiency compared with traditional TIRFM, adjustable illumination area, and simple switching between wide-field and TIRF imaging modes. TIRF images obtained with this approach are free of shadow artifacts and of interference fringes. Example micrographs of fluorescently labeled polystyrene beads, of Convallaria majalis tissue, and of Propidium-iodide-labeled Chinese hamster ovary cells are shown, and the capabilities of the scheme are discussed
Structured light sheet fluorescence microscopy based on four beam interference
A 3D structured light sheet microscope using a four-faceted symmetric pyramid is presented. The sample is illuminated by the resulting four beam interference field. This approach combines advantages of standing wave and structured illumination microscopy. Examples of micrographs of fluorescently labeled Chinese hamster ovary (CHO) cells as well as of the compound eyes of drosophila are shown and the optical sectioning ability of our system is demonstrated. The capabilities and the limitations of the scheme are discussed. (C) 2010 Optical Society of Americ
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