18 research outputs found
Interferon-beta induces S phase slowing via up-regulated expression of PML in squamous carcinoma cells.
Interferon-β induces S phase slowing via up-regulated expression of PML in squamous carcinoma cell
HIV-1 Nef Induces Proinflammatory State in Macrophages through Its Acidic Cluster Domain: Involvement of TNF Alpha Receptor Associated Factor 2
Background: HIV-1 Nef is a virulence factor that plays multiple roles during HIV replication. Recently, it has been described that Nef intersects the CD40 signalling in macrophages, leading to modification in the pattern of secreted factors that appear able to recruit, activate and render T lymphocytes susceptible to HIV infection. The engagement of CD40 by CD40L induces the activation of different signalling cascades that require the recruitment of specific tumor necrosis factor receptor-associated factors (i.e. TRAFs). We hypothesized that TRAFs might be involved in the rapid activation of NF-kappa B, MAPKs and IRF-3 that were previously described in Nef-treated macrophages to induce the synthesis and secretion of proinflammatory cytokines, chemokines and IFN beta to activate STAT1, -2 and -3. Methodology/Principal Findings: Searching for possible TRAF binding sites on Nef, we found a TRAF2 consensus binding site in the AQEEEE sequence encompassing the conserved four-glutamate acidic cluster. Here we show that all the signalling effects we observed in Nef treated macrophages depend on the integrity of the acidic cluster. In addition, Nef was able to interact in vitro with TRAF2, but not TRAF6, and this interaction involved the acidic cluster. Finally silencing experiments in THP-1 monocytic cells indicate that both TRAF2 and, surprisingly, TRAF6 are required for the Nef-induced tyrosine phosphorylation of STAT1 and STAT2. Conclusions: Results reported here revealed TRAF2 as a new possible cellular interactor of Nef and highlighted that in monocytes/macrophages this viral protein is able to manipulate both the TRAF/NF-kappa B and TRAF/IRF-3 signalling axes, thereby inducing the synthesis of proinflammatory cytokines and chemokines as well as IFN beta
Interferon-β induces cellular senescence in cutaneous human papilloma virus-transformed human keratinocytes by affecting p53 transactivating activity
Interferon (IFN)-beta inhibits cell proliferation and affects cell cycle in keratinocytes transformed by both mucosal high risk Human Papilloma Virus (HPV) and cutaneous HPV E6 and E7 proteins. In particular, upon longer IFN-beta treatments, cutaneous HPV38 expressing cells undergo senescence. IFN-beta appears to induce senescence by upregulating the expression of the tumor suppressor PML, a well known IFN-induced gene. Indeed, experiments in gene silencing via specific siRNAs have shown that PML is essential in the execution of the senescence programme and that both p53 and p21 pathways are involved. IFN-beta treatment leads to a modulation of p53 phosphorylation and acetylation status and a reduction in the expression of the p53 dominant negative Delta Np73. These effects allow the recovery of p53 transactivating activity of target genes involved in the control of cell proliferation. Taken together, these studies suggest that signaling through the IFN pathway might play an important role in cellular senescence. This additional understanding of IFN antitumor action and mechanisms influencing tumor responsiveness or resistance appears useful in aiding further promising development of biomolecular strategies in the IFN therapy of cancer
In vivo antitumor effect of an intracellular single-chain antibody fragment against the E7 oncoprotein of human papillomavirus 16.
Human papillomavirus (HPV)-associated tumors still represent an urgent problem of public health in spite of the efficacy of
the prophylactic HPV vaccines. Specific antibodies in single-chain format expressed as intracellular antibodies (intrabodies)
are valid tools to counteract the activity of target proteins. We previously showed that the M2SD intrabody, specific for the E7
oncoprotein of HPV16 and expressed in the endoplasmic reticulum of the HPV16-positive SiHa cells, was able to inhibit cell
proliferation. Here, we showed by confocal microscopy that M2SD and E7 colocalize in the endoplasmic reticulum of SiHa
cells, suggesting that the E7 delocalization mediated by M2SD could account for the anti-proliferative activity of the intrabody.
We then tested the M2SD antitumor activity in two mouse models for HPV tumors based respectively on TC-1 and C3 cells.
The M2SD intrabody was delivered by retroviral vector to tumor cells before cell injection into C57BL/6 mice. In both models,
a marked delay of tumor onset with respect to the controls was observed in all the mice injected with the M2SD-expressing
tumor cells and, importantly, a significant percentage of mice remained tumor-free permanently. This is the first in vivo demonstration
of the antitumor activity of an intrabody directed towards an HPV oncoprotein. We consider that these results could
contribute to the development of new therapeutic molecules based on antibodies in single-chain format, to be employed
against the HPV-associated lesions even in combination with other drugs
Thyroid Hormones Interaction With Immune Response, Inflammation and Non-thyroidal Illness Syndrome
The interdependence between thyroid hormones (THs), namely, thyroxine and
triiodothyronine, and immune system is nowadays well-recognized, although not yet fully
explored. Synthesis, conversion to a bioactive form, and release of THs in the circulation
are events tightly supervised by the hypothalamic–pituitary–thyroid (HPT) axis. Newly
synthesized THs induce leukocyte proliferation, migration, release of cytokines, and
antibody production, triggering an immune response against either sterile or microbial
insults. However, chronic patho-physiological alterations of the immune system, such
as infection and inflammation, affect HPT axis and, as a direct consequence, THs
mechanism of action. Herein, we revise the bidirectional crosstalk between THs and
immune cells, required for the proper immune system feedback response among diverse
circumstances. Available circulating THs do traffic in two distinct ways depending
on the metabolic condition. Mechanistically, internalized THs form a stable complex
with their specific receptors, which, upon direct or indirect binding to DNA, triggers
a genomic response by activating transcriptional factors, such as those belonging
to the Wnt/b-catenin pathway. Alternatively, THs engage integrin avb3 receptor on
cell membrane and trigger a non-genomic response, which can also signal to the
nucleus. In addition, we highlight THs-dependent inflammasome complex modulation
and describe new crucial pathways involved in microRNA regulation by THs, in
physiological and patho-physiological conditions, which modify the HPT axis and THs
performances. Finally, we focus on the non-thyroidal illness syndrome in which the
HPT axis is altered and, in turn, affects circulating levels of active THs as reported
in viral infections,particularly in immunocompromised patients infected with human
immunodeficiency virus
p53 and ΔNp73 co-localyze with IFN-β-induced PML into PML Nuclear Bodies.
<p>(A,B) For confocal microscopy analysis, K38 cells were cultured on glass bottom dishes (MatTek Corporation) and treated with IFN-β for 4 days. Cells were then fixed in PBS 4% paraformaldehyde for 30 min on ice, immuno-fluorescence labelling was performed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036909#s4" target="_blank">Materials and Methods</a> and sample were analyzed using confocal microscope (Leica TCS SP5).</p