The numerical analysis of piles\u27 restriction on foundation

Kako je moguće predvidjeti taloženje visokih zgrada i analizirati pomak temelja kako bi se zaštitile podzemne komunalije? To je urgentan problem u današnjoj izgradnji. U svrhu njegovog rješenja, u ovom se radu istražuje kako piloti utječu na vrijednost pomaka temelja primjenom Mohr-Coulomb Modela i 3D računarskog programa FLAC3D s 20 m dubokim iskopima temelja kao primjerom. Analiza pokazuje da je distribucijski uzorak pomaka temelja bez pilota konveksna krivulja, a da s pilotima krivulja postaje valovita uz porast vrijednosti pomaka temelja za 0,4÷0,6. Isto tako parametri poput udaljenosti pilota, promjera pilota i njihove dužine utječu na tu vrijednost na način da veći razmak snižava ograničenje pilota na vrijednost pomaka temelja. Stoga se u radu predlaže da najveći razmak između pilota ne bi trebao biti veći od 6d kad je ograničeni iznos vrijednosti oko 0,23÷0,37. Povećanje promjera i dužine pilota (ne više od 1,25 puta dubine kopanja) može učinkovito ograničiti pomak. Prema tome, u radu se daju korisni parametri za konstrukciju pilota, dokazuje njihovo djelovanje na mehanizam za ograničenje pomaka temelja i pruža analitička osnova za optimizaciju pilota.How can people predict the sedimentation of tall buildings and analyze the foundation rebound to protect underground utilities? This is the urgent problem in today\u27s construction. To solve it, this paper is dedicated to analyzing how piles affect the foundation rebound value by using Mohr-Coulomb Model and the 3D emulation calculation software FLAC3D with 20-meter-deep foundation pits as an example. The study shows that the distribution pattern of the foundation rebound without piles is a convex curve while piles make the curve go corrugated with the foundation rebound value shrieking by 0,4÷0,6. Also, parameters such as the distance of the piles and diameter of the piles and length of the piles have an effect on the value in a way that the increasing distance lowers the piles\u27 restriction on the foundation rebound value. Therefore, this paper suggests that the largest distance between piles should be no more than 6d when the restricted extent of the value is about 0,23÷0,37. Raising the diameter and length of the piles (no more than 1,25 times of the digging depth) can restrict rebound effectively. Therefore, this paper concludes useful parameters in pile design, proves its mechanism for restriction on foundation rebound, and provides analytical basis for optimization of the piles

Deep Learning based 3D Segmentation: A Survey

3D object segmentation is a fundamental and challenging problem in computer vision with applications in autonomous driving, robotics, augmented reality and medical image analysis. It has received significant attention from the computer vision, graphics and machine learning communities. Traditionally, 3D segmentation was performed with hand-crafted features and engineered methods which failed to achieve acceptable accuracy and could not generalize to large-scale data. Driven by their great success in 2D computer vision, deep learning techniques have recently become the tool of choice for 3D segmentation tasks as well. This has led to an influx of a large number of methods in the literature that have been evaluated on different benchmark datasets. This paper provides a comprehensive survey of recent progress in deep learning based 3D segmentation covering over 150 papers. It summarizes the most commonly used pipelines, discusses their highlights and shortcomings, and analyzes the competitive results of these segmentation methods. Based on the analysis, it also provides promising research directions for the future.Comment: Under review of ACM Computing Surveys, 36 pages, 10 tables, 9 figure

Current understanding of CTLA-4: from mechanism to autoimmune diseases

Autoimmune diseases (ADs) are characterized by the production of autoreactive lymphocytes, immune responses to self-antigens, and inflammation in related tissues and organs. Cytotoxic T-lymphocyte antigen 4 (CTLA-4) is majorly expressed in activated T cells and works as a critical regulator in the inflammatory response. In this review, we first describe the structure, expression, and how the signaling pathways of CTLA-4 participate in reducing effector T-cell activity and enhancing the immunomodulatory ability of regulatory T (Treg) cells to reduce immune response, maintain immune homeostasis, and maintain autoimmune silence. We then focused on the correlation between CTLA-4 and different ADs and how this molecule regulates the immune activity of the diseases and inhibits the onset, progression, and pathology of various ADs. Finally, we summarized the current progress of CTLA-4 as a therapeutic target for various ADs

Twist1 enhances hypoxia induced radioresistance in cervical cancer cells by promoting nuclear EGFR localization

Twist1 is a crucial transcription factor that regulates epithelial mesenchymal transition and involves in metastasis. Recent evidence suggests that Twist1 plays important role in hypoxia-induced radioresistance, but the underlying mechanism remains elusive. Here we investigated the change of Twist1 expression in human cervical squamous cancer cell line SiHa after hypoxia treatment. We also explored the role of Twist1 in radioresistance by manipulating the expression level of Twist1.We observed that hypoxia treatment elevated the expression of Twist1 in SiHa cells. Knockdown of Twist1 with siRNA increased the radiosensitivity of SiHa cells under hypoxia condition, accompanied by reduced levels of nuclear Epidermal Growth Factor Receptor (EGFR) and DNA-dependent protein kinase (DNA-PK). Conversely, overexpression of Twist1 led to increased radioresistance of SiHa cells, which in turn increased nuclear EGFR localisation and expression levels of nuclear DNA-PK. Moreover, concomitant high expression of hypoxia-inducible factor-1? (HIF-1?) and Twist1 in primary tumors of cervical cancer patients correlated with the worse prognosis after irradiation treatment. Taken together, these data provide new insights into molecular mechanism underlying hypoxia-induced radio resistance in cervical cancer cells, and suggest that Twist1 is a promising molecular target to improve the efficacy of cancer radiotherapy

HI content of massive red spiral galaxies observed by FAST

A sample of 279 massive red spirals was selected optically by Guo et al. (2020), among which 166 galaxies have been observed by the ALFALFA survey. In this work, we observe HI content of the rest 113 massive red spiral galaxies using the Five-hundred-meter Aperture Spherical radio Telescope (FAST). 75 of the 113 galaxies have HI detection with a signal-to-noise ratio (S/N) greater than 4.7. Compared with the red spirals in the same sample that have been observed by the ALFALFA survey, galaxies observed by FAST have on average a higher S/N, and reach to a lower HI mass. To investigate why many red spirals contain a significant amount of HI mass, we check color profiles of the massive red spirals using images observed by the DESI Legacy Imaging Surveys. We find that galaxies with HI detection have bluer outer disks than the galaxies without HI detection, for both ALFALFA and FAST samples. For galaxies with HI detection, there exists a clear correlation between galaxy HI mass and g-r color at outer radius: galaxies with higher HI masses have bluer outer disks. The results indicate that optically selected massive red spirals are not fully quenched, and the HI gas observed in many of the galaxies may exist in their outer blue disks.Comment: 11 pages, 9 figures, accepted by MNRAS; Table 1 is available in the source file

PRIMA-1Met suppresses colorectal cancer independent of p53 by targeting MEK

This work was supported by Grant No. 81201779 (Hua Xiong) from the National Natural Science Youth Foundation; Grant No. 81502118 (Yanmei Zou) from the National Natural Science Youth Foundation; Grant No. 2014CFB250 (Yanmei Zou) from the Natural Science Foundation of Hubei Province; Grant No. 81372434 (Huihua Xiong) from the National Natural Science Foundation.PRIMA-1Met is the methylated PRIMA-1 (p53 reactivation and induction of massive apoptosis) and could restore tumor suppressor function of mutant p53 and induce p53 dependent apoptosis in cancer cells harboring mutant p53. However, p53 independent activity of PRIMA-1Met remains elusive. Here we reported that PRIMA-1Met attenuated colorectal cancer cell growth irrespective of p53 status. Kinase profiling revealed that mitogen-activated or extracellular signal-related protein kinase (MEK) might be a potential target of PRIMA-1Met. Pull-down binding and ATP competitive assay showed that PRIMA-1Met directly bound MEK in vitro and in cells. Furthermore, the direct binding sites of PRIMA-1Met were explored by using a computational docking model. Treatment of colorectal cancer cells with PRIMA-1Met inhibited p53-independent phosphorylation of MEK, which in turn impaired anchorage-independent cell growth in vitro. Moreover, PRIMA-1Met suppressed colorectal cancer growth in xenograft mouse model by inhibiting MEK1 activity. Taken together, our findings demonstrate a novel p53-independent activity of PRIMA-1Met to inhibit MEK and suppress colorectal cancer growth.Publisher PDFPeer reviewe

Arsenic and Cadmium Accumulation in Soil as Affected by Continuous Organic Fertilizer Application: Implications for Clean Production

As and Cd in soil can be assimilated and accumulated by vegetables and can be subsequently ingested by humans. Contradictory effects of organic fertilizer application on As and Cd accumulation in soil have been reported in previous studies. An eight-year greenhouse study was conducted on a sandy loam soil in Beijing, China to investigate the effects of organic fertilizer application rate on soil properties, and As and Cd accumulation in soil. The contamination risk of pak choi grown after eight years’ application of organic fertilizer was also evaluated. Soil organic carbon increased 3.0–3.8 times with low, medium and high rates of fertilizer application in 2018 compared to the initial soil. Organic fertilizer application significantly increased soil nutrients and microbial biomass while it mildly affected soil pH. The bioavailability of As/Cd has decreased after eight years’ application of organic fertilizer. Pak choi crop harvested from all three treatments in 2018 did not pose a threat to human health, even for life-time consumption. Soil total As content significantly decreased with organic fertilizer application, mainly due to the lower As content in the applied fertilizer than that in soil. Continuous application of clean organic fertilizer can be adopted to reduce the contamination risk of highly contaminated soil in the soil–plant system

Defining the Genetic Features of O-Antigen Biosynthesis Gene Cluster and Performance of an O-Antigen Serotyping Scheme for Escherichia albertii

Escherichia albertii is a newly described and emerging diarrheagenic pathogen responsible for outbreaks of gastroenteritis. Serotyping plays an important role in diagnosis and epidemiological studies for pathogens of public health importance. The diversity of O-antigen biosynthesis gene clusters (O-AGCs) provides the primary basis for serotyping. However, little is known about the distribution and diversity of O-AGCs of E. albertii strains. Here, we presented a complete sequence set for the O-AGCs from 52 E. albertii strains and identified seven distinct O-AGCs. Six of these were also found in 15 genomes of E. albertii strains deposited in the public database. Possession of wzy/wzx genes in each O-AGC strongly suggest that O-antigens of E. albertii were synthesized by the Wzx/Wzy-dependent pathway. Furthermore, we performed an O-antigen serotyping scheme for E. albertii based on specific antisera against seven O-antigens and a high throughput xTAG Luminex assay to simultaneously detect seven O-AGCs. Both methods accurately identified serotypes of 64 tested E. albertii strains. Our data revealed the high-level diversity of O-AGCs in E. albertii. We also provide valuable methods to reliably identify and serotype this bacterium

Potential source contributions and risk assessment of PAHs in sediments from the tail-reaches of the Yellow River Estuary, China: PCA model, PMF model, and mean ERM quotient analysis

Principal component analysis (PCA), positive matrix factorization (PMF), and the mean effects range-median quotient (MERM-Q) models were employed to determine occurrence levels, sources, and potential toxicological significance of polycyclic aromatic hydrocarbons (PAHs) in surface sediments of the Yellow River Estuary, China. Due to the grain size of sediments, cumulative effects, and distribution of oil fields, the total concentration of the 16 U.S. Environmental Protection Agency (US EPA) priority PAHs (T-PAHs) measured in sediments along transects in the offshore area was 119.51 +/- 39.58 ng g(-1) dry weight (dw), which is notably higher than that measured in rivers (75.00 +/- 12.56 ng g(-1) dw) and estuaries (67.94 +/- 10.20 ng g(-1) dw). PAH levels decreased seaward along all the studied transects in coastal Bohai Bay. Multivariate statistical analyses supported that PAHs in sediments were principally derived from coal and biomass combustion, oil pollution, and vehicular emissions. Based on the MERM-Q (0.0050 +/- 0.0017), PAHs were at low potential of ecotoxicological contamination level. These results provide helpful information for protecting water resources and serving sustainable development in Construction of Ecological Civilization in the Yellow River Delta

Sources and historical sedimentary record: Temporal variability of n-alkane and PAHs from the Yellow River Estuary, China

The composition and distribution of n-alkanes and polycyclic aromatic hydrocarbons (PAHs) in suspended particulate matter (SPM) from the Yellow River and a sedimentary core from the Yellow River Estuary, China, were measured in order to determine environmental changes in the Yellow River Estuary on a regional scale. The concentration of n-alkane in SPM increased along the upper-middle-lower reaches of the Yellow River. The total concentrations of n-alkanes and PAHs in the core ranged from 0.04 to 0.86 mu g g(-1) (avg. 0.21 mu g g(-1)), and from 0.04 to 0.29 mu g g(-1) (avg. 0.15 mu g g(-1)) on a dry wt. basis, respectively. Understanding the temporal evolution of n-alkanes provide information on terrigenous versus aquatic productivity, oil exploration at the Shengli Oilfield, and channel diversion in the Yellow River. n-Alkanes in SPM were mainly derived from mixed sources, with terrigenous inputs dominating. PAHs in the sediment core were predominantly derived from coal and biomass combustion. The variation in PAHs levels throughout the core determines changes in energy use and socio-economic development. The temporal variability in n-alkane and PAHs and their molecular diagnostic ratios revealed a trend of regional environmental change and the role of anthropogenic activity in that environmental change