4 research outputs found

    Effect of elevated temperatures on mechanical properties of ultra high strength hot work tool steel h11

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    This paper presents an experimental and numerical study into the influence of elevated temperatures on mechanical properties of the heat treated high quality hot work tool steel H11. This steel belongs to a group of alloyed steels with extraordinary mechanical properties. The aim of this study was to determine the highest temperature at which these properties are still maintained. The experimental investigation focused on the tensile testing of specimens at seven different temperatures, including the room temperature. The highest testing temperature was 700 degrees C. The heat treatment of plates (specimens) consisted of quenching and tempering. Although the strain hardening of this type of materials is small, the strain hardening curves were calculated to show if there was a possibility for the material to increase its strength due to exploitation loads. Also, a numerical analysis of the tensile test by using the finite element method was done in order to define an appropriate model for numerical testing. The obtained results are then compared with the experimental results

    Experimental-numerical study of tensile strength of the high-strength steel S690QL at elevated temperatures

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    © 2016 Springer Science+Business Media New York. We present the experimental-numerical analysis of the influence of temperature on mechanical properties of structural high-strength steel class S690QL. Since the steel S690QL belongs to a group of steels with good mechanical properties, the aim of this paper is to determine the highest temperatures at which these good characteristics are kept. Experimental tensile testings of the specimens were performed at five different temperatures in the range from 20 to 550°C. Beside experimental testing, strengthening curves were calculated and numerical analysis using finite element method was performed. Both the experimental and numerical results have shown that decrease of mecahanical properties occurs at approximately 450°C

    Effect of elevated temperatures on mechanical properties of ultra high strength hot work tool steel H11

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    © 2020 Transactions of Famena. All rights reserved. This paper presents an experimental and numerical study into the influence of elevated temperatures on mechanical properties of the heat treated high quality hot work tool steel H11. This steel belongs to a group of alloyed steels with extraordinary mechanical properties. The aim of this study was to determine the highest temperature at which these properties are still maintained. The experimental investigation focused on the tensile testing of specimens at seven different temperatures, including the room temperature. The highest testing temperature was 700 °C. The heat treatment of plates (specimens) consisted of quenching and tempering. Although the strain hardening of this type of materials is small, the strain hardening curves were calculated to show if there was a possibility for the material to increase its strength due to exploitation loads. Also, a numerical analysis of the tensile test by using the finite element method was done in order to define an appropriate model for numerical testing. The obtained results are then compared with the experimental results

    Conformational maps of human 20S proteasomes reveal PA28- and immuno-dependent inter-ring crosstalks

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    International audienceHydrogen-Deuterium eXchange coupled to Mass Spectrometry (HDX-MS) is now common practice in structural biology. However, it is most of the time applied to rather small oligomeric complexes. Here, we report on the use of HDX-MS to investigate conformational differences between the human standard 20S (std20S) and immuno 20S (i20s) proteasomes alone or in complex with PA28αβ or PA28γ activators. Their solvent accessibility is analyzed through a dedicated bioinformatic pipeline including stringent statistical analysis and 3D visualization. These data confirm the existence of allosteric differences between the std20S and i20S at the surface of the α-ring triggered from inside the catalytic β-ring. Additionally, binding of the PA28 regulators to the 20S proteasomes modify solvent accessibility due to conformational changes of the β-rings. This work is not only a proof-of-concept that HDX-MS can be used to get structural insights on large multi-protein complexes in solution, it also demonstrates that the binding of the std20S or i20S subtype to any of its PA28 activator triggers allosteric changes that are specific to this 20S/PA28 pair
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