Hojení defektu femorální kosti pomocí dvou nových biokompatibilních rozložitelných materiálů

This study was conducted as an in vivo experiment in adult miniature pigs with the aim to test two new biomaterials. An iatrogenic defect was made into the central femoral diaphysis in the experimental animals and subsequently fixated by bridging plate osteosynthesis. Into the defect we implanted a cancellous autograft (control group), a pasty injectable scaffold (EXP A), anda porous 3D cylinder (EXP B). Radiological examination was performed in all animals at 0, 10, 20, 30 weeks after surgical procedure and histological assessment was performed. In the newly formed bone the osteoblastic activity was monitored. In terms of radiology, the most effective method was observed in the control group (completely healed 100%) compared to experimental groups EXP A (70.0%) and EXP B (62.5%).Tato studie byla provedena jako experiment in vivo na dospělých miniaturních prasatech s cílem otestovat dva nové biomateriály. U centrálních femorálních diafýz byl u experimentálních zvířat proveden iatrogenní defekt, který byl následně fixován přemostěním osteosyntézy dlahy. Do defektu jsme implantovali spongiózní autotransplantát (kontrolní skupina), injektabilní skafold (EXP A) a porézní 3D válec (EXP B). Radiologické vyšetření bylo provedeno u všech zvířat v 0, 10, 20, 30 týdnech po chirurgickém zákroku a bylo provedeno histologické hodnocení. V nově vytvořené kosti byla sledována osteoblastická aktivita. Pokud jde o radiologii, nejúčinnější metoda byla pozorována u kontrolní skupiny (zcela uzdravená 100%) ve srovnání s experimentálními skupinami EXP A (70,0%) a EXP B (62,5%)

Epigenetic factors in breast cancer therapy

Funding Information: The authors would like to acknowledge the support from their respective institutes throughout the review writing process. Publisher Copyright: Copyright © 2022 Mathur, Jha, Saini, Jha, Shukla, Filipejová, Kesari, Iqbal, Nand, Upadhye, Jha, Roychoudhury and Slama.Epigenetic modifications are inherited differences in cellular phenotypes, such as cell gene expression alterations, that occur during somatic cell divisions (also, in rare circumstances, in germ line transmission), but no alterations to the DNA sequence are involved. Histone alterations, polycomb/trithorax associated proteins, short non-coding or short RNAs, long non—coding RNAs (lncRNAs), & DNA methylation are just a few biological processes involved in epigenetic events. These various modifications are intricately linked. The transcriptional potential of genes is closely conditioned by epigenetic control, which is crucial in normal growth and development. Epigenetic mechanisms transmit genomic adaptation to an environment, resulting in a specific phenotype. The purpose of this systematic review is to glance at the roles of Estrogen signalling, polycomb/trithorax associated proteins, DNA methylation in breast cancer progression, as well as epigenetic mechanisms in breast cancer therapy, with an emphasis on functionality, regulatory factors, therapeutic value, and future challenges.Peer reviewe