Recombinant Pseudomonas Vaccine: Technological Aspects of Obtaining and Evaluating Quality Indicators

For an aim to prevent Pseudomonas aeruginosa, a candidate recombinant vaccine has been developed. This vaccine – (RPV) was based on two protective proteins of P. aeruginosa: the outer membrane protein F (OprF) and the recombinant truncated form of the Exotoxin A (toxoid) that were adsorbed on the aluminum hydroxide. The optimal immunization schedule for mice included two intraperitoneal administrations with a two-week interval. RPV promoted to increase survival rates in challenged immunized mice and stimulated humoral and innate immune responses. During preclinical studies, we confirmed the immunogenicity of the vaccine that had not pyrogenicity, acute and chronic toxicity, allergenicity and immunotoxicity. Keywords: Pseudomonas aeruginosa, outer membrane protein F (OprF), toxoid, Pseudomonas Recombinant Vaccine (PRV

Changes in the Anxiety of Moscow Students during the COVID-19 Pandemic

This work is devoted to assessing the changes in anxiety among Moscow students during the COVID-2019 pandemic. The material was collected in comprehensive examination of 18–25-year-old students studying at Lomonosov Moscow State University (residents of Moscow and the Moscow region). The study included longitudinal and cross-sectional components. The methodology included a social questionnaire about the peculiarities of the self-isolation regime, living conditions of the respondent, professional employment, etc. and the scale of situational and personal anxiety of C.D. Spielberger in the Russian-language adaptation of Yu.L. Khanin. A total of 284 questionnaires have been analyzed. In 2020, two consecutive waves of infection were recorded in Russia: in May and in December. During the first wave, severe social restrictions and a temporary lockdown were introduced in Moscow, and the second one took place under conditions of significantly less restrictions, although it was marked by a large number of infections. Different social load and adaptation to the pandemic led to the fact that the behavioral manifestations of stress and the level of situational anxiety among Moscow students increased significantly at the beginning of the pandemic, but subsequently began to decrease. It can be concluded that among university students there is a social and psychological adaptation to new norms of life, which are dictated by the conditions of the COVID-19 pandemic

A review of the genus Muusoctopus (Cephalopoda: Octopoda) from Arctic waters

We report two Arctic species of incirrate octopods new to science. One is formally described here as Muusoctopus aegir Golikov, Gudmundsson & Sabirov sp. nov. while the other, Muusoctopus sp. 1, is not formally described due to a limited number of samples (all are immature individuals). These two species differ from each other, and from other Muusoctopus, especially in: 1) absence of stylets (in M. aegir sp. nov.); 2) proportions of mantle and head; 3) funnel organ morphology (W-shaped with medial and marginal limbs of equal length in M. aegir sp. nov., or medial are slightly longer; V V-shaped with medial limbs slightly longer and broader than marginal in Muusoctopus sp. 1); 4) sucker and gill lamellae counts; 5) relative arm length and sucker diameter; and 6) male reproductive system relative size and morphology. Species of Muusoctopus now comprise four of 12 known Arctic cephalopods. Additionally, this study provides: a) new data on the morphology and reproductive biology of M. johnsonianus and M. sibiricus, and a diagnosis of M. sibiricus; b) the equations to estimate mantle length and body mass from beak measurements of M. aegir sp. nov. and M. johnsonianus; c) a cytochrome c oxidase subunit I gene barcode for M. sibiricus; d) new data on the ecology and distribution of all studied species; and e) a data table for the identification of northern North Atlantic and Arctic species of Muusoctopus