Visualización del proceso de enseñanza-aprendizaje, apoyado de una interfaz gráfica

186 páginas. Doctorado en DiseñoEn esta tesis se presenta un conjunto de requerimientos obtenidos a partir de un análisis metodológico, con el objetivo de conformar un sistema educativo, haciendo énfasis en el diseño de una interfaz gráfica, en lo sucesivo IG que permita el proceso de enseñanza- aprendizaje de la matemática, en el dominio de las ecuaciones de primer grado con una incógnita. Para tal propósito fue necesario que se abordarán distintos enfoques metodológicos pertenecientes al campo de: 1) Los modelos para el diseño de una interfaz gráfica, 2) Los modelos instruccionales, tomando en consideración las inteligencias múltiples de Gardner, 3) Las metodologías de desarrollo de software educativ

Recombinant Fragments of Antibodies

6. CoNct usloN The aim of the thesis was to establish, in the national conditions, technology of preparation of antibody reconrbinant fragrnents and to verífo the cornplete procedure using several model monoclonal antibodies with a potential diagnostic and therapeutic use. For tfuee antibodies' mAb TU-20, M75 and MEM97, recombinant |Ťagments in various formats (scFv fragments monovalent and bivalent, i.e. diabody, and intrabody for intracellular expression) were constructed and for their heterologous expression, vectors allowing expression in E. coli (as cytoplasmic inclusions, periplasmic inclusions and in soluble form) and in Drosophila 32 cells (expression of glycosylated forms of scFv fragments into the medium) were used. In case of proteins expressed in irrsoluble form, especially scFv F|1.2.32, renaturation procedures to obtain active scFv fragments were developed and optimized. The efnect of the length of the linker -(GlyrSer).- (where x is I to 4) connecting the variable domains of the light and heavy chain on the formation of different multimeric forms of scFv rvas studied. For obtaining solell monomeric scFv fragment, the length of 20 amino acid residues tumsd out optimal. Fragnrents rvith a linker l5 residues long. formed a mixture of monomers, dimers and trimers. the proportion of rvhich rvas..

Media 6: LCD-based digital eyeglass for modulating spatial-angular information

Originally published in Optics Express on 04 May 2015 (oe-23-9-11813

Visualization 1: InstantScope: a low-cost whole slide imaging system with instant focal plane detection

Media 1 Originally published in Biomedical Optics Express on 01 September 2015 (boe-6-9-3210

Visualization 2: InstantScope: a low-cost whole slide imaging system with instant focal plane detection

Media 2 Originally published in Biomedical Optics Express on 01 September 2015 (boe-6-9-3210

Stiffness analysis of 3D spheroids using microtweezers - Fig 3

<p>(A) The experimental set up with a well containing a spheroid. B. The cantilever tips were immersed into the media to access the spheroid (arrow) to apply step compression.</p

Stiffness analysis of 3D spheroids using microtweezers - Fig 1

<p>A. Microtweezer system showing the three main components: tweezer arms with a flexible plate spring, a bimorph piezo actuator and two cantilevers as the force sensing tips. The moving arm was actuated (direction indicated by the arrow) to move the cantilever closer to the fixed arm. B. The cantilever holder along with SU8 micro-cantilevers. C. Micro-cantilevers were made from SU8 and brass to obtain a wide measureable force range of sub hundred nN to more than 1 mN.</p

Microindentation set up for mechanical characterization of agarose using stainless steel sphere and 100g load cell.

<p>Microindentation set up for mechanical characterization of agarose using stainless steel sphere and 100g load cell.</p

Spheroid stiffness analysis was done using SU8 cantilevers.

<p>Three cell lines BT474, T47D and MCF 10A spheroids were tested. The protocol similar to agarose pillar was applied for each cell type. Red square indicates reference and green square indicates the position of the cantilever tip with sample. The deviation in the green and red square in A4, B4, and C4 shows cantilever bending due to the spheroid. A1, B1 and C1 being the initial position for each cell line. Panel 1–4 represents step 0, 6, 12 and 18, respectively, for cantilevers. Supplementary movie clips of BT474 (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0188346#pone.0188346.s002" target="_blank">S2 Movie</a>), T47D (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0188346#pone.0188346.s003" target="_blank">S3 Movie</a>) and MCF10A (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0188346#pone.0188346.s004" target="_blank">S4 Movie</a>) compression are available.</p

Two materials were used to make replaceable cantilever tips.

<p>The stiffness and force range for each cantilever is indicated.</p