A novel multifunctional biomedical material based on polyacrylonitrile:preparation and characterization

Wet spun microfibers have great potential in the design of multifunctional controlled release materials. Curcumin (Cur) and vitamin E acetate (Vit. E Ac) were used as a model drug system to evaluate the potential application of the drug-loaded microfiber system for enhanced delivery. The drugs and polyacrylonitrile (PAN) were blended together and spun to produce the target drug-loaded microfiber using an improved wet-spinning method and then the microfibers were successfully woven into fabrics. Morphological, mechanical properties, thermal behavior, drug release performance characteristics, and cytocompatibility were determined. The drug-loaded microfiber had a lobed “kidney” shape with a height of 50 ~ 100 μm and width of 100 ~ 200 μm. The addition of Cur and Vit. E Ac had a great influence on the surface and cross section structure of the microfiber, leading to a rough surface having microvoids. X-ray diffraction and Fourier transform infrared spectroscopy indicated that the drugs were successfully encapsulated and dispersed evenly in the microfilament fiber. After drug loading, the mechanical performance of the microfilament changed, with the breaking strength improved slightly, but the tensile elongation increased significantly. Thermogravimetric results showed that the drug load had no apparent adverse effect on the thermal properties of the microfibers. However, drug release from the fiber, as determined through in-vitro experiments, is relatively low and this property is maintained over time. Furthermore, in-vitro cytocompatibility testing showed that no cytotoxicty on the L929 cells was found up to 5% and 10% respectively of the theoretical drug loading content (TDLC) of curcumin and vitamin E acetate. This study provides reference data to aid the development of multifunctional textiles and to explore their use in the biomedical material field

Seroprevalence of Toxoplasma gondii infection in pet dogs in Lanzhou, Northwest China

<p>Abstract</p> <p>Background</p> <p>In recent years, surveys of <it>Toxoplasma gondii </it>infection in dogs have been reported worldwide, including China. However, little is known about the prevalence of <it>T. gondii </it>in pet dogs in Northwest China. In the present study, the prevalence of <it>T. gondii </it>in pet dogs in Lanzhou, China was investigated using the modified agglutination test (MAT).</p> <p>Results</p> <p>In this survey, antibodies to <it>T. gondii </it>were found in 28 of 259 (10.81%) pet dogs, with MAT titers of 1:20 in 14 dogs, 1:40 in nine, 1:80 in four, and 1:160 or higher in one dog. The prevalence ranged from 6.67% to 16.67% among dogs of different ages, with low rates in young pet dogs, and high rates in older pet dogs. The seroprevalence in dogs >3 years old was higher than that in dogs ≤1 years old, but the difference was not statistically significant (<it>P ></it>0.05). The seroprevalence in male dogs was 12.50% (17 of 136), and in female dogs it was 8.94% (11 of 123), but the difference was not statistically significant (<it>P ></it>0.05).</p> <p>Conclusions</p> <p>A high prevalence of <it>T. gondii </it>infection was found in pet dogs in Lanzhou, Northwest China, which has implications for public health in this region. In order to reduce the risk of exposure to <it>T. gondii</it>, further measures and essential control strategies should be carried out rationally in this region.</p

Development of clinically relevant orthotopic xenograft mouse model of metastatic lung cancer and glioblastoma through surgical tumor tissues injection with trocar

<p>Abstract</p> <p>Objective</p> <p>Orthotopic models are important in cancer research. Here we developed orthotopic xenograft mouse model of metastatic lung cancer and glioblastoma with a specially designed system.</p> <p>Methods</p> <p>Tiny fragments of surgical tumors were implanted into the mice brain with a trocar system. Immunohistochemistry was performed to detect brain tumor stem cells among glioblastoma tissues, including both the original and resulting ones with monoclonal antibody against CD133.</p> <p>Results</p> <p>Besides the constant high take rates in both models; brain transplants perfectly resembled their original tumors in biological behaviors. The brain tumor stem cells, positively stained with CD133 were found, though not frequently, in both original and resulting glioblastoma tissues.</p> <p>Conclusions</p> <p>Orthotopic model established with a trocar system is effective and injection of tumor tissues containing stem cells promise the forming of new tumor mass when grafted.</p

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals &lt;1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Reaction Kinetics of Hydrogen Atom Abstraction from C4–C6 Alkenes by the Hydrogen Atom and Methyl Radical

Alkenes are important ingredients of realistic fuels and are also critical intermediates during the combustion of a series of other fuels including alkanes, cycloalkanes, and biofuels. To provide insights into the combustion behavior of alkenes, detailed quantum chemical studies for crucial reactions are desired. Hydrogen abstractions of alkenes play a very important role in determining the reactivity of fuel molecules. This work is motivated by previous experimental and modeling evidence that current literature rate coefficients for the abstraction reactions of alkenes are still in need of refinement and/or redetermination. In light of this, this work reports a theoretical and kinetic study of hydrogen atom abstraction reactions from C4–C6 alkenes by the hydrogen (H) atom and methyl (CH₃) radical. A series of C4–C6 alkene molecules with enough structural diversity are taken into consideration. Geometry and vibrational properties are determined at the B3LYP/6-31G­(2df,p) level implemented in the Gaussian-4 (G4) composite method. The G4 level of theory is used to calculate the electronic single point energies for all species to determine the energy barriers. Conventional transition state theory with Eckart tunneling corrections is used to determine the high-pressure-limit rate constants for 47 elementary reaction rate coefficients. To faciliate their applications in kinetic modeling, the obtained rate constants are given in the Arrhenius expression and rate coefficients for typical reaction classes are recommended. The overall rate coefficients for the reaction of H atom and CH₃ radical with all the studied alkenes are also compared. Branching ratios of these reaction channels for certain alkenes have also been analyzed

Bio-inspired three-dimensional self-patterning of functional coatings for PDMS microfluidics

We report a facile, versatile and highly efficient self-patterning method for depositing functional coatings for poly(dimethylsiloxane) (PDMS) microfluidics, inspired by the wetting phenomena of the desert beetle and plant leaves. Driven by the solvent evaporation, fluoropolymer coatings have been demonstrated to deposit only on the roughened PDMS surfaces with exact coverage, conformal thickness, no clogging and good bonding strength, which significantly suppressed the swelling of PDMS by the organic solvent and the nonspecific adsorption of the fluorescent dye. © 2013 The Royal Society of Chemistry

Synthetical Optimization of a Gravity-Driven Irrigation Pipeline Network System with Pressure-Regulating Facilities

Due to the influence of topographic drops, a large elevation difference often occurs in the middle and lower sections of the main pipe of a gravity-driven irrigation pipe network (GDIPN) system. This elevation difference must be reduced appropriately through pressure reduction facilities (pressure-regulating ponds (PRPs) or pressure-reducing valves (PRVs)). The number and locations of PRPs are crucial factors in regulating and balancing the pressure head of the main pipe of a GDIPN system as well as in reducing the project cost. However, there are few studies on the optimization of this kind of pipe network system. In this paper, first, we generalize such type of GDIPN system, and a simplified mathematical model for such system optimization was established. A genetic algorithm based on a fixed proportion and direct comparison (GA-FPDC) was introduced to solve the model. Two existing projects were tested by the proposed method. The results show that the presented method not only improved the design efficiency and rationality but also greatly decreased the project cost. The presented method is effective and efficient to address optimization design of such GDIPN system problems

The orphan nuclear receptor TR3/Nur77 regulates ER stress and induces apoptosis via interaction with TRAP gamma

Fundamental Research Funds for the Central Universities, China [2010121096]; Fujian Provincial Department of Science and Technology [2012J01148]; National Natural Science Foundation of China [31000620]; Ministry of Education, China [B06016]The orphan nuclear receptor TR3 (also known as Nur77) belongs to the steroid/thyroid/retinoid nuclear receptor superfamily and plays important roles in regulating cell proliferation, differentiation and apoptosis. No physiological ligand for TR3 has been found thus far; the determination of its binding partners is therefore important to clarify the biological functions of TR3. Here, we identified translocon-associated protein subunit gamma (TRAP gamma) as a novel TR3 binding partner using a tandem affinity purification method. This interaction between TR3 and TRAP gamma was further confirmed, and the interacting regions were mapped. The ligand-binding domain of TR3 was required for TRAP gamma binding, and the C terminus of TRAP gamma was responsible for its interaction with TR3. When stimulated with 12-O-tetradecanoylphorbol 13-acetate (TPA) or CD437, this TR3-TRAP gamma interaction not only induced Ca2+ depletion in the endoplasmic reticulum (ER) but also promoted the expression of the proapoptotic transcriptional regulator CHOP. Notably, both TR3 and TRAP gamma were required for ER stress-induced apoptosis in HepG2 cells. Overall, this study demonstrated a novel, TR3-initiated signaling pathway in which TR3 regulates ER stress and induces apoptosis of hepatoma cells through its interaction with TRAP gamma. (c) 2013 Elsevier Ltd. All rights reserved