Influence of β-Stabilizers on the α-Ti→ω-Ti Transformation in Ti-Based Alloys

The development of next generation Ti-based alloys demand completely new processes and approaches. In particular, the Ti-alloys of next generation will contain not only α-Ti and β-Ti phases, but also small amounts of ω-phase and intermetallic compounds. The β→ω phase transformation induced by high-pressure torsion (HPT) has been studied in detail recently. In this work, we investigated the HPT-induced α→ω phase transformation. For this purpose, we added various β-stabilizers into α-Ti matrix of studied Ti-alloys. Ti-alloys with 4% Fe, 2% Cr, 3% Ni, and 4% Co (wt. %) have been annealed at the temperatures below their point of eutectoid decomposition, from β-Ti to α-Ti, and respective intermetallics (TiFe, Ti$_{2}$Co, Ti$_{2}$Ni, TiCr$_{2}$). Volume fraction of HPT-driven ω-phase (from ≤5 up to ~80%) depended on the amount of alloying element dissolved in the α-matrix. Evaluation of lattice parameters revealed accelerated mass transfer during HPT at room temperature corresponding to bulk diffusion in α-Ti at ~600 °С

Inflammatory Proteins HMGA2 and PRTN3 as Drivers of Vulvar Squamous Cell Carcinoma Progression.

Current knowledge on the biology of squamous cell vulvar carcinoma (VSCC) is limited. We aimed to identify protein markers of VSCC tumors that would permit to stratify patients by progression risk. Early-stage tumors from patients who progressed (progVSCC) and from those who were disease-free (d-fVSCC) during follow-up, along with normal vulvar tissues were examined by mass spectrometry-based proteomics. Differentially expressed proteins (DEPs) were then verified in solid tissues and blood samples of patients with VSCC tumors and vulvar premalignant lesions. In progVSCC vs. d-fVSCC tumors, the immune response was the most over-represented Gene Ontology category for the identified DEPs. Pathway profiling suggested bacterial infections to be linked to aggressive VSCC phenotypes. High Mobility Group AT-Hook 2 (HMGA2) and Proteinase 3 (PRTN3) were revealed as proteins predicting VSCC progression. HMGA2 and PRTN3 abundances are associated with an aggressive phenotype, and hold promise as markers for VSCC patient stratification. It appears that vulvovaginal microflora disturbances trigger an inflammatory response contributing to cancer progression, suggesting that bacterial rather than viral infection status should be considered in the development of targeted therapies in VSCC

miR-125b-5p, miR-155-3p, and miR-214-5p and target E2F2 gene in oral squamous cell carcinoma

It is known that E2F2 (E2F transcription factor 2) plays an important role as controller in the cell cycle. This study aimed to analyse the expression of the E2F2 gene and E2F2 protein and demonstrate E2F2 target microRNAs (miRNAs) candidates (miR-125b-5p, miR-155-3p, and miR-214-5p) in oral squamous cell carcinoma tumour and margin samples. The study group consisted 50 patients. The E2F2 gene and miRNAs expression levels were assessed by qPCR, while the E2F2 protein was assessed by ELISA. When analysing the effect of miRNAs expression on E2F2 gene expression and E2F2 protein level, we observed no statistically significant correlations. miR-125b-5p was downregulated, while miR-155-3p, and miR-214-5p were upregulated in tumour samples compared to margin. We observed a difference between the miR-125b-5p expression level in smokers and non-smokers in margin samples. Furthermore, HPV-positive individuals had a significantly higher miR-125b-5p and miR-214-5p expression level compared to HPV-negative patients in tumour samples. The study result showed that the E2F2 gene is not the target for analysed miRNAs in OSCC. Moreover, miR-155-3p and miR-125b-5p could play roles in the pathogenesis of OSCC. A differential expression of the analysed miRNAs was observed in response to tobacco smoke and HPV status

miR-125b-5p, miR-155-3p, and miR-214-5p and target E2F2 gene in oral squamous cell carcinoma

It is known that E2F2 (E2F transcription factor 2) plays an important role as controller in the cell cycle. This study aimed to analyse the expression of the E2F2 gene and E2F2 protein and demonstrate E2F2 target microRNAs (miRNAs) candidates (miR-125b-5p, miR-155-3p, and miR-214-5p) in oral squamous cell carcinoma tumour and margin samples. The study group consisted 50 patients. The E2F2 gene and miRNAs expression levels were assessed by qPCR, while the E2F2 protein was assessed by ELISA. When analysing the effect of miRNAs expression on E2F2 gene expression and E2F2 protein level, we observed no statistically significant correlations. miR-125b-5p was downregulated, while miR-155-3p, and miR-214-5p were upregulated in tumour samples compared to margin. We observed a difference between the miR-125b-5p expression level in smokers and non-smokers in margin samples. Furthermore, HPV-positive individuals had a significantly higher miR-125b-5p and miR-214-5p expression level compared to HPV-negative patients in tumour samples. The study result showed that the E2F2 gene is not the target for analysed miRNAs in OSCC. Moreover, miR-155-3p and miR-125b-5p could play roles in the pathogenesis of OSCC. A differential expression of the analysed miRNAs was observed in response to tobacco smoke and HPV status

The 42nd Symposium Chromatographic Methods of Investigating Organic Compounds : Book of abstracts

The 42nd Symposium Chromatographic Methods of Investigating Organic Compounds : Book of abstracts. June 4-7, 2019, Szczyrk, Polan

Language functions in web advertisements (based on examples from ads concerning animals).

W mojej pracy licencjackiej zajmuję się ogłoszeniami internetowymi oraz funkcjami, jakie w nich występują. W pierwszym rozdziale charakteryzuję ogłoszenie jak tekst, w drugim omawiam prace na temat funkcji językowych, w trzecim analizuję ogłoszenia właśnie pod kątem występujących w nich funkcji. Ogłoszenia te są dosyć specyficzne, gdyż to samo zwierzę przedstawia siebie i swoją historię, nie robi tego jego właściciel. Dodatkowo, nietypowe jest to, że dominuje w nich funkcja impresywna, a nie poznawcza. W analizowanych przeze mnie tekstach występują cztery funkcje : prezentatywna, ekspresywna, impresywna oraz fatyczna.In my thesis I analyse web advertisements and their functions. In the first chapter I describe advertisements like a text, in the second I talk over works about language functions and in the third I analyse advertisements in view of their functions. These advertisements are unique because animal introduce itself and his history. Moreover, it is specific that in these advertisements dominate the impressive function, not the cognitive function. In these advertisements occur four functions: cognitive, expressive, impressive and phatic

Stereotypes of the wolf in works for children

Centrum zainteresowania mojej pracy magisterskiej stanowi wykazanie, w jaki sposób zbudowany jest stereotyp wilka w tekstach dla dzieci oraz jak wygląda on na tle kulturowym i językowym. Pierwszy rozdział zawiera przegląd pojęć stereotypu, głównie w ujęciu językoznawczym oraz krótką charakterystykę badań nad stereotypami u dzieci. W drugim rozdziale omawiam wyobrażenie wilka w kontekście kulturowym, na który składa się warstwa językowa, psychoanaliza oraz wierzenia, przesądy i religie. Trzeci rozdział przedstawia charakterystykę wyobrażenia mitologicznego wilka, które składa się ze stereotypowego obrazu wilka oraz z cech wypływających z tła kulturowego i językowego. Ostatni, czwarty rozdział ma na celu zobrazowanie stereotypu wilka ukazanego w utworach dla dzieci. Analizie zostały poddane utwory znane i najbardziej charakterystyczne, a zostało ona wykonana metodą fasetową. Stereotypowy obraz wilka obejmował cechy nazywające, wartościujące, zawierające komponent przestrzenny oraz takie, które umożliwią rozpoznanie go.Focus of my thesis is to demonstrate how they built a stereotype wolf in children's texts and how he looks on cultural and linguistic background. The first chapter provides an overview of the concepts of stereotype, mainly in terms of linguistics and a brief description of research into stereotypes of children. In the second chapter I discuss the idea of a wolf in a cultural context, which consists of a layer of language, psychoanalysis and beliefs, superstitions and religions. The third section presents the characteristics of a wolf mythological ideas, which consists of the stereotypical image of the wolf and the characteristics stemming from cultural and linguistic background. The last, fourth chapter aims to illustrate the wolf stereotype shown in works for children. The analysis have been submitted to works known and most characteristic, and it was made using faceted. The stereotypical image of the wolf included calling features, evaluative, including spatial component and those that enable the identification of him

Ostre rozlane zapalenie ucha zewnętrznego

Diffuse acute otitis externa (OEAD) is one of the most frequent cases recorded in Otorhinolaryngological emergency room. The main symptoms include: acute otalgia, feeling of ear fullness, itching, hearing impairment, sometimes fever. Otoscopy is the basic part of the diagnosis. First-line treatment involves topical antibiotic eardrops with or without a steroid. Clinicians should prescribe a non-ototoxic preparation when the patient has a known or suspected perforation of the tympanic membrane. Systemic antibiotics should not be prescribed in the first stage of the therapy unless the patient is at risk of severe course of the disease.Ostre rozlane zapalenie ucha zewnętrznego (OEAD; otitis externa acuta diffusa) to jedna z częstszych przyczyn zgłaszania się pacjentów na ostry dyżur otorynolaryngologiczny. Głównymi dolegliwościami, o których informują chorzy, są: nagły ostry ból, uczucie pełności w uchu, często świąd, upośledzenie słuchu, czasami gorączka. Podstawą rozpoznania jest badanie otoskopowe. Leczenie pierwszego rzutu polega na podawaniu miejscowo kropli zawierających antybiotyk ze sterydem (np. cyprofloksacyna z fluocynolonem) lub bez (np. cyprofloksacyna). Jeżeli nie ma możliwości oceny ciągłości błony bębenkowej, należy unikać stosowania preparatów o potencjale ototoksycznym. Antybiotyki o działaniu systemowym nie powinny być przepisywane w pierwszej fazie leczenia zapalenia ucha zewnętrznego – chyba, że pacjent jest w grupie ryzyka wystąpienia zapalenia ucha zewnętrznego o ciężkim przebiegu

Frequency and Severity of Ear–Nose–Throat (ENT) Symptoms during COVID-19 Infection

Background and Objectives: Coronavirus disease 2019 (COVID-19) is a new disease entity caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The main symptoms of infection at the onset of the pandemic include dyspnea, cough and high fever. Ear–nose–throat (ENT) symptoms are among the ones presented by patients in the course of infection. The aim of the study was to analyze the frequency of ENT symptoms and to assess their severity and duration in COVID-19 patients. Materials and Methods: The study included 337 patients who had been infected with SARS-CoV-2, as confirmed by a PCR test. The study participants were >18 years old; the mean age was 43.98 years ± 13.47 SD. The convalescents completed a questionnaire that contained 26 questions, including 9 detailed questions related to ENT symptoms, such as sore throat, vertigo, dizziness, hearing disorders, olfactory disorders, taste disturbance, headache, cough and dyspnea. The severity of symptoms was assessed using a Visual Analogue Scale (VAS). Results: The most reported ENT symptoms were olfactory disorders, which occurred in 72% of patients. The second most frequent symptom was taste disturbance (68%), VAS = 6.79 ± 3.01. Vertigo and dizziness were reported by 34% of respondents (VAS = 4.01 ± 2.01). Tinnitus was observed in 15% of patients, VAS = 3.87 ± 1.98; 14% of the subjects reported hearing impairment (VAS = 3.81 ± 2.37). Conclusions: Symptoms related to the sense of smell, taste and hearing are some of the most common symptoms in the course of COVID-19, which is important in the therapeutic and epidemiological management of patients. Delayed diagnosis and treatment of symptoms, especially those related to the hearing organ, may result in greater permanent damage

Analiza objawów ogólnoustrojowych w przebiegu COVID-19

INTRODUCTION: COVID-19 (coronavirus disease 2019), a disease caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) infection, may have a more or less severe course. The aim of this study was to analyse the symptoms which occurred in a group of people who tested positively for the presence of the genetic material of the virus. MATERIAL AND METHODS: The analysis was based on the results of 337 questionnaires collected from Polish patients – 185 (55%) females and 152 (45%) males aged 18–86. The questionnaire included 26 questions, of which 11 were related to systemic symptoms. The frequency of their occurrence and their severity were determined according to Visual Analogue Scale (VAS). RESULTS: The most common symptoms that occurred in over 50% of the respondents included: general weakness (91% of the respondents, severity of 6.25 on VAS), musculoskeletal pain (77%, VAS 5.67), headache (65%, VAS 5.37) and cough (56%, VAS 4.76). Runny nose, fever above 38℃ and sore throat were less common and occurred in 39% of the subjects. The least frequent symptoms were diarrhoea (23%), skin lesions (7%) and vomiting (3%). When determining the correlation coefficient, it was also found that dyspnoea occurred most often with general weakness (r = 1), whereas general weakness was less frequently associated with musculoskeletal pain (r = 0.8). No statistically significant relationships were found between the frequency of the symptoms and age, gender or the presence of comorbidities in the respondents. CONCLUSIONS: The observations showed that in the analysed group of people with SARS-CoV-2 infection, the disease had a mild-to-moderate course. General weakness, musculoskeletal pain, cough, and upper respiratory tract infection symptoms were the most common.WSTĘP: COVID-19 (coronavirus disease 2019), choroba wywołana infekcją SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2), może charakteryzować się mniej lub bardziej nasilonym przebiegiem. Celem niniejszego opracowania była analiza objawów, jakie wystąpiły w grupie osób z dodatnim wynikiem testu na obecność materiału genetycznego wirusa. MATERIAŁ I METODY: Analizę przeprowadzono na podstawie wyników ankiety przeprowadzonej wśród 337 polskich pacjentów – 185 (55%) kobiet oraz 152 (45%) mężczyzn w wieku 18–86 lat. Kwestionariusz zawierał 26 pytań, z czego 11 dotyczyło objawów ogólnoustrojowych. Określono częstość ich występowania oraz nasilenie w skali VAS (Visual Analogue Scale). WYNIKI: Do najczęstszych objawów, występujących u ponad 50% ankietowanych, należą: ogólne osłabienie (91% ankietowanych, nasilenie 6,25 w skali VAS), bóle układu mięśniowo-szkieletowego (77%, VAS 5,67), bóle głowy (65%, VAS 5,37) oraz kaszel (56%, VAS 4,76). Chorobie rzadziej towarzyszyły katar, gorączka powyżej 38°C oraz bóle gardła, które wystąpiły u 39% pacjentów. Najrzadziej występowały biegunka (23%), zmiany skórne (7%) oraz wymioty (3%). Określając współczynnik korelacji, stwierdzono również, iż duszności występowały najczęściej z ogólnym osła-bieniem (r = 1), natomiast rzadziej ogólnemu osłabieniu towarzyszyły bóle mięśniowo-szkieletowe (r = 0,8). Nie stwierdzono statystycznie istotnych zależności między częstością występowania danych objawów a wiekiem, płcią czy chorobami współistniejącymi u ankietowanych. WNIOSKI: Obserwacje wykazały, iż w analizowanej grupie zakażonych SARS-CoV-2 choroba miała przebieg łagodny lub umiarkowany. Najczęstszymi objawami były: ogólne zmęczenie, bóle mięśniowo-szkieletowe, kaszel oraz objawy nieżytu górnych dróg oddechowych