Исследование алгоритмов идентификации параметров модели в адаптивной системе управления экструдером

Показано, що під час процесу екструзії в апараті відбуваються різноманітні процеси, які обумовлюють нестаціонарні властивості апарата, як об’єкта керування. Показана доцільність використання адаптивних систем керування процесом екструзії. Досліджені алгоритми ідентифікації параметрів математичної моделі та зроблені висновки щодо ефективності того чи іншого алгоритму.It is rotined that during the process of extrusion in a vehicle there are various processes which stipulate non-stationary properties of vehicle. Rotined expedience of the use of adaptive control system by the process of extrusion. Researching observer identifiers parameters of mathematical model and done conclusions in relation to efficiency of one or another algorithm.Показано, что во время процесса экструзии в аппарате происходят разнообразные процессы, которые обусловливают нестационарные свойства аппарата, как объекта управления. Показанная целесообразность использования адаптивных систем управления процессом экструзии. Исследованы алгоритмы идентификации параметров математической модели и сделанные выводы относительно эффективности того или иного алгоритма

Pharmacological screening antihypoxic and cytoprotective properties of mexidol and analogs of human erythropoietin in cultured leukocytes of pigs

The article outlines improved methodological approaches to preclinical screening of antihypoxic and cytoprotective drugs. To investigate the effect of Mexidol, carbamylated darbepoetin, darbepoetin, and placebo (isotonic solution for the substance) against the background of hypoxic and cytotoxic effects on cell culture. Get data on the cytoprotective and antihypoxic therapeutic corridor of Mexido

Pharmacological screening antihypoxic and cytoprotective properties of mexidol and analogs of human erythropoietin in cultured leukocytes of pigs

The article outlines improved methodological approaches to preclinical screening of antihypoxic and cytoprotective drugs. To investigate the effect of Mexidol, carbamylated darbepoetin, darbepoetin, and placebo (isotonic solution for the substance) against the background of hypoxic and cytotoxic effects on cell culture. Get data on the cytoprotective and antihypoxic therapeutic corridor of Mexido

The cinnamyl alcohol dehydrogenase gene family is involved in the response to Fusarium oxysporum in resistant and susceptible flax genotypes

Flax (Linum usitatissimum L.) is used for the production of textile, oils, pharmaceuticals, and composite materials. Fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. lini, is a very harmful disease that reduces flax production. Flax cultivars that are resistant to Fusarium wilt have been developed, and the genes that are involved in the host response to F. oxysporum have been identified. However, the mechanisms underlying resistance to this pathogen remain unclear. In the present study, we used transcriptome sequencing data obtained from susceptible and resistant flax genotypes grown under control conditions or F. oxysporum infection. Approximately 250 million reads, generated with an Illumina NextSeq instrument, were analyzed. After filtering to exclude the F. oxysporum transcriptome, the remaining reads were mapped to the L. usitatissimum genome and quantified. Then, the expression levels of cinnamyl alcohol dehydrogenase (CAD) family genes, which are known to be involved in the response to F. oxysporum, were evaluated in resistant and susceptible flax genotypes. Expression alterations in response to the pathogen were detected for all 13 examined CAD genes. The most significant differences in expression between control and infected plants were observed for CAD1B, CAD4A, CAD5A, and CAD5B, with strong upregulation of CAD1B, CAD5A, and CAD5B and strong downregulation of CAD4A. When plants were grown under the same conditions, the expression levels were similar in all studied flax genotypes for most CAD genes, and statistically significant differences in expression between resistant and susceptible genotypes were only observed for CAD1A. Our study indicates the strong involvement of CAD genes in flax response to F. oxysporum but brings no evidence of their role as resistance gene candidates. These findings contribute to the understanding of the mechanisms underlying the response of flax to F. oxysporum infection and the role of CAD genes in stress resistance

Two novel missense mutations in the myelin protein zero gene causes Charcot-Marie-Tooth type 2 and Déjérine-Sottas syndrome

<p>Abstract</p> <p>Background</p> <p>The Charcot-Marie-Tooth (CMT) phenotype caused by mutation in the <it>myelin protein zero (MPZ) </it>gene varies considerably, from early onset and severe forms to late onset and milder forms. The mechanism is not well understood. The myelin protein zero (P₀) mediates adhesion in the spiral wraps of the Schwann cell's myelin sheath. The crystalline structure of the extracellular domain of the myelin protein zero (P₀ex) is known, while the transmembrane and intracellular structure is unknown.</p> <p>Findings</p> <p>One novel missense mutation caused a milder late onset CMT type 2, while the second missense mutation caused a severe early onset phenotype compatible with Déjérine-Sottas syndrome.</p> <p>Conclusions</p> <p>The phenotypic variation caused by different missense mutations in the <it>MPZ </it>gene is likely caused by different conformational changes of the MPZ protein which affects the functional tetramers. Severe changes of the MPZ protein cause dysfunctional tetramers and predominantly uncompacted myelin, i.e. the severe phenotypes congenital hypomyelinating neuropathy and Déjérine-Sottas syndrome, while milder changes cause the phenotypes CMT type 1 and 2.</p

Association analysis of a highly polymorphic CAG Repeat in the human potassium channel gene KCNN3 and migraine susceptibility

BACKGROUND: Migraine is a polygenic multifactorial disease, possessing environmental and genetic causative factors with multiple involved genes. Mutations in various ion channel genes are responsible for a number of neurological disorders. KCNN3 is a neuronal small conductance calcium-activated potassium channel gene that contains two polyglutamine tracts, encoded by polymorphic CAG repeats in the gene. This gene plays a critical role in determining the firing pattern of neurons and acts to regulate intracellular calcium channels. METHODS: The present association study tested whether length variations in the second (more 3') polymorphic CAG repeat in exon 1 of the KCNN3 gene, are involved in susceptibility to migraine with and without aura (MA and MO). In total 423 DNA samples from unrelated individuals, of which 202 consisted of migraine patients and 221 non-migraine controls, were genotyped and analysed using a fluorescence labelled primer set on an ABI310 Genetic Analyzer. Allele frequencies were calculated from observed genotype counts for the KCNN3 polymorphism. Analysis was performed using standard contingency table analysis, incorporating the chi-squared test of independence and CLUMP analysis. RESULTS: Overall, there was no convincing evidence that KCNN3 CAG lengths differ between Caucasian migraineurs and controls, with no significant difference in the allelic length distribution of CAG repeats between the population groups (P = 0.090). Also the MA and MO subtypes did not differ significantly between control allelic distributions (P > 0.05). The prevalence of the long CAG repeat (>19 repeats) did not reach statistical significance in migraineurs (P = 0.15), nor was there a significant difference between the MA and MO subgroups observed compared to controls (P = 0.46 and P = 0.09, respectively), or between MA vs MO (P = 0.40). CONCLUSION: This association study provides no evidence that length variations of the second polyglutamine array in the N-terminus of the KCNN3 channel exert an effect in the pathogenesis of migraine

Genetics of migraine and pharmacogenomics: some considerations

Migraine is a complex disorder caused by a combination of genetic and environmental factors

Химический состав приземного атмосферного аэрозоля в Баренцбурге (архипелаг Шпицберген) по результатам многолетних исследований

The chemical composition (ions, elements, polycyclic aromatic hydrocarbons) of aerosol and gaseous impurities (SO2, HNO3, HCl, NH3) in the surface layer of the atmosphere in Barentsburg, located on the Western Svalbard island (Svalbard archipelago), is analyzed. Atmospheric aerosol and gaseous impurities brought to the Arctic from middle latitudes and deposited on snow and ice not only interact with various natural objects, but also spread to long distances with melting dirty snow and ice. Air sampling was carried out following to methodology adopted by the international networks of the atmospheric monitoring programs in South-East Asia (EANET) and Europe (EMEP). In 2011-2015, the observations of the chemical composition of the atmospheric ground layer were performed daily during the light season (April–September), and monthly from April 2016 to 2018. The largest total ion concentrations were observed in 2011–2012. Seasonal variability of ion concentrations in the aerosol was characterized by high values in the cold period (October–February) and low values in the warm one (May–June). High values of the coefficient of correlation between ions Na+ and Cl− (r = 0,93) as well as between Mg2+ and Cl−  (r = 0,81) throughout the year show that the main source of the aerosol is the sea surface. The significant correlation between ions K+, NO3-, NH4+, SO42−, K+, SO42− in the polar night point to the influence of local sources: coal mining at the mine and its3 combustion at thermal power plants. Emission of polycyclic aromatic hydrocarbons and the gaseous impurities (SO2, HNO3) into the atmosphere, especially during the polar night, is also influenced by local sources. Among the elements the maximum enrichment of the aerosol was revealed for As, Cr, Zn, Mo, Cd, Sn, Sb, W, and Pb with a low content of Cd, Sn, Sb, W, and Pb in the coal, sludge and on the underlying surface. On the basis of the elemental composition of the aerosol and the back-trajectory analysis, it was shown that the air masses enriched in heavy metals come to the area of the Barentsburg settlement from middle latitudes.Прослежена межгодовая (2011–2017 гг.) и сезонная (2016–2018 гг.) изменчивость компонентов химического состава (ионы, элементы, полициклические ароматические углеводороды) атмосферного аэрозоля и газообразных примесей (SO2, HNO3, HCl, NH3) в приземной атмосфере западной части Российской Арктики (пос. Баренцбург на Шпицбергене)

The Ataxic Cacna1a-Mutant Mouse Rolling Nagoya: An Overview of Neuromorphological and Electrophysiological Findings

Homozygous rolling Nagoya natural mutant mice display a severe ataxic gait and frequently roll over to their side or back. The causative mutation resides in the Cacna1a gene, encoding the pore-forming α1 subunit of Cav2.1 type voltage-gated Ca2+ channels. These channels are crucially involved in neuronal Ca2+ signaling and in neurotransmitter release at many central synapses and, in the periphery, at the neuromuscular junction. We here review the behavioral, histological, biochemical, and neurophysiological studies on this mouse mutant and discuss its usefulness as a model of human neurological diseases associated with Cav2.1 dysfunction