Remote Sensing Evidence for Significant Variations in the Global Gross Domestic Product during the COVID-19 Epidemic

Coronavirus disease 2019 (COVID-19) has been spreading rapidly and is still threatening human health currently. A series of measures for restraining epidemic spreading has been adopted throughout the world, which seriously impacted the gross domestic product (GDP) globally. However, details of the changes in the GDP and its spatial heterogeneity characteristics on a fine scale worldwide during the pandemic are still uncertain. We designed a novel scheme to simulate a 0.1° × 0.1° resolution grid global GDP map during the COVID-19 pandemic. Simulated nighttime-light remotely sensed data (SNTL) was forecasted via a GM(1, 1) model under the assumption that there was no COVID-19 epidemic in 2020. We constructed a geographically weighted regression (GWR) model to determine the quantitative relationship between the variation of nighttime light (ΔNTL) and the variation of GDP (ΔGDP). The scheme can detect and explain the spatial heterogeneity of ΔGDP at the grid scale. It is found that a series of policies played an obvious role in affecting GDP. This work demonstrated that the global GDP, except for in a few countries, represented a remarkably decreasing trend, whereas the ΔGDP exhibited significant differences

Transplantation of Porcine Hepatocytes Cultured with Polylactic Acid-O-Carboxymethylated Chitosan Nanoparticles Promotes Liver Regeneration in Acute Liver Failure Rats

In this study, free porcine hepatocytes suspension (Group A), porcine hepatocytes embedded in collagen gel (Group B), porcine hepatocytes cultured with PLA-O-CMC nanoparticles and embedded in collagen gel (Group C), and PLA-O-CMC nanoparticles alone (Group D) were transplanted into peritoneal cavity of ALF rats, respectively. The result showed that plasma HGF levels were elevated post-transplantation with a peak at 12 hr. The rats in Group C showed highest plasma HGF levels at 2, 6, 12, 24 and 36 hr post-transplantation and lowest HGF level at 48 hr. Plasma VEGF levels were elevated at 48 hr post-transplantation with a peak at 72 hr. The rats in Group C showed highest plasma HGF levels at 48, 72, and 96 hr post-transplantation. The liver functions in Group C were recovered most rapidly. Compared with Group B, Group C had significant high liver Kiel 67 antigen labeling index (Ki-67 LI) at day 1 post-HTx (P < .05). Ki-67 LI in groups B and C was higher than that in groups A and D at days 5 and 7 post-HTx. In conclusion, intraperitoneal transplantation of porcine hepatocytes cultured with PLA-O-CMC nanoparticles and embedded in collagen gel can promote significantly liver regeneration in ALF rats

Identifying Population Hollowing Out Regions and Their Dynamic Characteristics across Central China

Continuous urbanization and industrialization lead to plenty of rural residents migrating to cities for a living, which seriously accelerated the population hollowing issues. This generated series of social issues, including residential estate idle and numerous vigorous laborers migrating from undeveloped rural areas to wealthy cities and towns. Quantitatively determining the population hollowing characteristic is the priority task of realizing rural revitalization. However, the traditional field investigation methods have obvious deficiencies in describing socio-economic phenomena, especially population hollowing, due to weak efficiency and low accuracy. Here, this paper conceives a novel scheme for representing population hollowing levels and exploring the spatiotemporal dynamic of population hollowing. The nighttime light images were introduced to identify the potential hollowing areas by using the nightlight decreasing trend analysis. In addition, the entropy weight approach was adopted to construct an index for evaluating the population hollowing level based on statistical datasets at the political boundary scale. Moreover, we comprehensively incorporated physical and anthropic factors to simulate the population hollowing level via random forest (RF) at a grid-scale, and the validation was conducted to evaluate the simulation results. Some findings were achieved. The population hollowing phenomenon decreasing gradually was mainly distributed in rural areas, especially in the north of the study area. The RF model demonstrated the best accuracy with relatively higher R2 (Mean = 0.615) compared with the multiple linear regression (MLR) and the geographically weighted regression (GWR). The population hollowing degree of the grid-scale was consistent with the results of the township scale. The population hollowing degree represented an obvious trend that decreased in the north but increased in the south during 2016–2020 and exhibited a significant reduction trend across the entire study area during 2019–2020. The present study supplies a novel perspective for detecting population hollowing and provides scientific support and a first-hand dataset for rural revitalization

Synthesis, Biological Evaluation of Fluorescent 23-Hydroxybetulinic Acid Probes, and Their Cellular Localization Studies

© 2018 American Chemical Society. 23-Hydroxybetulinic acid (23-HBA) is a complex lupane triterpenoid, which has attracted increasing attention as an anticancer agent. However, its detailed mechanism of anticancer action remains elusive so far. To reveal its anticancer mode of action, a series of fluorescent 23-HBA derivatives conjugated with coumarin dyes were designed, synthesized, and evaluated for their antiproliferative activities. Subcellular localization and uptake profile studies of representative fluorescent 23-HBA probe 26c were performed in B16F10 cells, and the results suggested that probe 26c was rapidly taken up into B10F10 cells in a dose-dependent manner and mitochondrion was the main site of its accumulation. Further mode of action studies implied that the mitochondrial pathway was involved in 23-HBA-mediated apoptosis. Together, our results provided new clues for revealing the molecular mechanism of natural product 23-HBA for its further development into an antitumor agent

Bis{tris­[3-(2-pyrid­yl)-1H-pyrazole]iron(II)} tetra­deca­molybdo(V,VI)silicate

The asymmetric unit of the title compound, [Fe(C8H7N3)3]2[SiMo14O44], consists of a complex [Fe(C8H7N3)3]2+ cation and half of a derivative of an α-Keggin-type anion, [SiMo14O44]4−. In the mixed-valent MoV/VI anion, the α-Keggin type core is capped on two oppositely disposed tetra­gonal faces by additional (MoO2) units. The [SiMo14O44]4− anion shows disorder. Two O atoms of the central SiO4 group ( symmetry) are equally disordered about an inversion centre. Moreover, two of the outer bridging O atoms and the O atoms of the capping (MoO2) unit are likewise disordered. The Fe2+ cation is surrounded in a slightly distorted octa­hedral coordination by six N atoms from three 3-(2-pyrid­yl)-1H-pyrazole ligands. N—H⋯O hydrogen bonding between the cations and anions leads to a consolidation of the structure

Infection of inbred BALB/c and C57BL/6 and outbred Institute of Cancer Research mice with the emerging H7N9 avian influenza virus

A new avian-origin influenza virus A (H7N9) recently crossed the species barrier and infected humans; therefore, there is an urgent need to establish mammalian animal models for studying the pathogenic mechanism of this strain and the immunological response. In this study, we attempted to develop mouse models of H7N9 infection because mice are traditionally the most convenient models for studying influenza viruses. We showed that the novel A (H7N9) virus isolated from a patient could infect inbred BALB/c and C57BL/6 mice as well as outbred Institute of Cancer Research (ICR) mice. The amount of bodyweight lost showed differences at 7 days post infection (d.p.i.) (BALB/c mice 30%, C57BL/6 and ICR mice approximately 20%), and the lung indexes were increased both at 3 d.p.i. and at 7 d.p.i.. Immunohistochemistry demonstrated the existence of the H7N9 viruses in the lungs of the infected mice, and these findings were verified by quantitative real-time polymerase chain reaction (RT-PCR) and 50% tissue culture infectious dose (TCID50) detection at 3 d.p.i. and 7 d.p.i.. Histopathological changes occurred in the infected lungs, including pulmonary interstitial inflammatory lesions, pulmonary oedema and haemorrhages. Furthermore, because the most clinically severe cases were in elderly patients, we analysed the H7N9 infections in both young and old ICR mice. The old ICR mice showed more severe infections with more bodyweight lost and a higher lung index than the young ICR mice. Compared with the young ICR mice, the old mice showed a delayed clearance of the H7N9 virus and higher inflammation in the lungs. Thus, old ICR mice could partially mimic the more severe illness in elderly patients. </p

Immune cell early activation, apoptotic kinetic, and T-cell functional impairment in domestic pigs after ASFV CADC_HN09 strain infection

African swine fever (ASF) caused by the African swine fever virus (ASFV) is a fatal and highly contagious disease of domestic pigs characterized by rapid disease progression and death within 2 weeks. How the immune cells respond to acute ASFV infection and contribute to the immunopathogenesis of ASFV has not been completely understood. In this study, we examined the activation, apoptosis, and functional changes of distinct immune cells in domestic pigs following acute infection with the ASFV CADC_HN09 strain using multicolor flow cytometry. We found that ASFV infection induced broad apoptosis of DCs, monocytes, neutrophils, and lymphocytes in the peripheral blood of pigs over time. The expression of MHC class II molecule (SLA-DR/DQ) on monocytes and conventional DCs as well as CD21 expression on B cells were downregulated after ASFV infection, implying a potential impairment of antigen presentation and humoral response. Further examination of CD69 and ex vivo expression of IFN-γ on immune cells showed that T cells were transiently activated and expressed IFN-γ as early as 5 days post-infection. However, the capability of T cells to produce cytokines was significantly impaired in the infected pigs when stimulated with mitogen. These results suggest that the adaptive cellular immunity to ASFV might be initiated but later overridden by ASFV-induced immunosuppression. Our study clarified the cell types that were affected by ASFV infection and contributed to lymphopenia, improving our understanding of the immunopathogenesis of ASFV

Enhanced Bauxite Recovery Using a Flotation Column Packed with Multilayers of Medium

An innovative self-designed medium was packed in a bench-scale flotation column to study its influence on the flotation recovery of bauxite. Computational fluid dynamics (CFD) simulation was conducted to reveal the impact of the packing medium on the turbulent characteristics of collection zone in the column. Simulation results show that multilayer packing of the medium divides the collection zone into small units having different turbulent intensities, which is more suitable for flotation separation. The packing medium decreases the turbulence kinetic energy (from 1.08 &times; 10&minus;2 m2/s2 to 2.1 &times; 10&minus;3 m2/s2), turbulence eddy dissipation (from 3.71 &times; 10&minus;2 m2/s3 to 9.8 &times; 10&minus;3 m2/s3) and axial fluid velocity of fluid in the column. With three layers of packing, the recovery of Al2O3 increased by 2.11% and the aluminum to silicon content ratio of the concentrate improved from 5.16 to 9.72

Bis{tris[3-(2-pyridyl)-1H-pyrazole]zinc(II)} dodecamolybdosilicate hexahydrate

The asymmetric unit of the title compound, [Zn(C8H7N3)3]2[SiMo12O40]&amp;#183;6H2O, consists of a complex [Zn(C8H7N3)3]2+ cation, half of a Keggin-type [SiMo12O40]4&amp;#8722; heteropolyanion and three uncoordinated water molecules. The Zn2+ cation is surrounded in a distorted octahedral coordination by six N atoms from three chelating 3-(2-pyridyl)pyrazole ligands. In the heteropolyanion, two O atoms of the central SiO4 group (overline{1} symmetry) are equally disordered about an inversion centre. N&amp;#8212;H...O hydrogen bonding between the cations, anions and the uncoordinated water molecules leads to a consolidation of the structure