Photonic crystal Microarray Nanoplatform for High Throughput Detection of Biomolecules

ABSTRACT We present preliminary designs and experimental results for creating a microarray nanoplatform based on twodimensional photonic crystal devices in silicon. Multiple photonic crystal microcavities are coupled along the length of a single photonic crystal waveguide that undergo resonance wavelength shifts when an antibody-antibody binding event occurs in the immediate vicinity of the corresponding photonic crystal microcavity. The microarray nanoplatform enables high throughput measurements of multiple antibody-antibody interactions via a single optical waveguide transmission measurement

A laboratory study of the expiratory airflow and particle dispersion in the stratified indoor environment

Understanding the role of human expiratory flows on respiratory infection in ventilated environments is useful for taking appropriate interventions to minimize the infection risk. Some studies have predicted the lock-up phenomenon of exhaled flows in stratified environments; however, there is a lack of high-quality experimental data to validate the theoretical models. In addition, how thermal stratification affects the transport of exhaled particles has not been explored so far. In this study, a water tank experiment was conducted according to the similarity protocols to mimic how the expiratory airflow and particles behaved in both uniform and stratified environments. The lock-up phenomenon was visualized and compared with the predicted results by an integral model. Results showed that our previously developed theoretical model of a respiratory airflow was effective to predict the airflow dispersion in stratified environments. Stratification frequency (N) of the background fluid and the Froude Number 〖"Fr" 〗_"0" of the thermal flow jointly determined the lock-up layer in a power law. For the particle dispersion, it indicated that small particles such as fine droplets and droplet nuclei would be ‘locked’ by indoor thermal stratification, and disperse with the thermal flow over a long distance, potentially increasing the long-range airborne infection risk. Large particles such as large droplets can deposit within a short distance, hardly affected by thermal stratification, however, droplet infection could happen to the susceptible people at a close contact with the infector. This study could give some guidance in view of cross-infection control indoors for stratified environment

A Simple and Improved Model for Describing Soil Hydraulic Properties from Saturation to Oven Dryness

Soil water retention characteristics and hydraulic conductivity from saturation to oven dryness are needed for simulating soil water movement and solute transport, especially in arid and semiarid regions. The aim of this study was to derive a new soil hydraulic model and compare this model with the classic van Genuchten–Mualem (VGM) models (M1). A total of 12 soils with varying basic properties were selected from the Unsaturated Soil Hydraulic Database (UNSODA) for the evaluation of the new model. Five pairs of soil hydraulic models were considered, including M1 and the new model with different parameters fitted (M2–M5). Correlation analysis was conducted to analyze the relationships between the new model parameters and basic soil properties. In addition, the Morris method was applied to quantify the parameter sensitivity of the new model. Results showed that M2 to M5 gave slightly better performance than M1 in predicting soil water retention characteristics, with a decrease of ∼15% in the root mean squared error (RMSE). However, they produced substantially better performance than M1 in estimating soil hydraulic conductivities, with a decrease of ∼40% in the RMSE. The new model with all of the model parameters being fitted (M2) was more efficient than those with one or two model parameters not fitted. However, an over-parameterization problem was detected with M2. Physically unrealistic parameters (e.g., the saturated soil water content and effective grain diameter) exist in M2. These parameters were found to have important influences on the results of the soil hydraulic properties prediction based on the Morris-based sensitivity analysis. Overall, the new model with two parameters not fitted (M5) can best describe the soil hydraulic properties from saturation to oven dryness and had the potential to simulate water and solute transport in the vadose zone

HspB5 correlates with poor prognosis in colorectal cancer and prompts epithelial-mesenchymal transition through ERK signaling.

Alpha B-crystallin (HspB5) is abnormally expressed in tumor tissues and portends a poor prognosis in cancer patients. However, the role of HspB5 in colorectal cancer (CRC) is still unclear. Seventy CRC patients and 40 healthy volunteers were sampled from August 2012 to March 2015 in order to determine the clinical significance of HspB5. In vitro cellular studies were used to validate its molecular mechanisms in CRC. Our clinical data indicated that HspB5 was up-regulated, and had a positive association with TNM stage CRC patients. The expression level of HspB5 in CRC patients was closely correlated with MMP7 and E-cadherin, two core epithelial-mesenchymal transition (EMT) gene products. The in vitro studies revealed that high HspB5 expression could prompt tumor cell proliferation and invasion, as well as EMT. Gene-microarray analysis suggested three significant signaling pathways (PI3K, p38 and ERK) were involved in HspB5-induced EMT. Signal transduction pathway inhibitors and HspB5 gene knockdown models suggested that HspB5 promotes CRC tumorigenesis and EMT progression through ERK signaling pathways. In summary, HspB5 maybe trigger the EMT in CRC by activating the ERK signaling pathway. It is a potential tumor biomarker for CRC diagnosis and prognosis

Urban microclimates and urban heat island in Chongqing, China

Chongqing is the largest directly-controlled municipality in China, which is now undergoing a rapid urbanization. The urbanization rate increased from 35.6% in 2000 to 48.3% in 2007, and it is estimated to reach at least 70% by 2020. The question remains open: What are the consequences of such rapid urbanization in Chongqing in terms of urban microclimate? Furthermore, Chongqing is located within the Three Gorges Reservoir (TGR) region and the upper Yangtze River, where the Three Gorges Reservoir (TGR) project started in 1993 and was completed in 2010. As one of the biggest construction projects in the world with a rising water level of 175m and water storage capacity of about 39.3 billion m3, it would be interesting to investigate how such a gigantic project impacts the surrounding micro-environment, especially in Chongqing. Different research approaches are adopted in the study. Our literature review indicates present studies on the urban climate in Chongqing are mainly confined within the historical trend analysis of several weather stations operated by the Chongqing government, little is known about the spatial distribution of urban air temperature and how the local land cover influences the air temperature, especially when there are rivers running through the Chongqing urban area. To contribute to the present knowledge, a series of field measurement campaigns and numerical simulations were carried out. Two complementary types of field measurements are included: fixed weather stations and mobile transverse measurement. Numerical simulations using a house-developed program are able to predict the urban air temperature in Chongqing

Metal-semiconductor-metal Ge photodetectors on SOI substrates for near infrared wavelength operation

Conference Name:5th IEEE International Conference on Group IV Photonics. Conference Address: Sorrento, ITALY. Time:SEP 17-19, 2008.Metal-semiconductor-metal Ge photodetectors on SOI substrates operating at 1.3-1.6 mu m have been demonstrated. Ge films with thin top silicon layer for suppressing dark current were grown on low-temperature buffers by ultra-high vacuum chemical vapor deposition