Analysis of dilemma and countermeasures of patent transformation in colleges and universities in Shaanxi Province

To explore and implement the diversifi cation of intellectual property ownership and benefi t distribution is the main theme of the era of knowledge economy. In recent years, Shaanxi colleges and universities have done a lot of work in improving the intellectual property system and promoting the transformation of scientifi c and technological achievements, and have achieved positive results, but there is still a big gap compared with many colleges and universities in advanced areas in China. This paper starts from the current situation of patent transformation in colleges and universities in Shaanxi, analyzes the main problems in patent transformation, namely the diffi culties faced, and puts forward targeted suggestions to improve the patent transformation mechanism in colleges and universities in Shaanxi, and provides reference for promoting the patent transformation work in colleges and universities in Shaanxi by formulating effective countermeasures

The amoebal MAP kinase response to Legionella pneumophila is regulated by DupA

SummaryThe amoeba Dictyostelium discoideum can support replication of Legionella pneumophila. Here we identify the dupA gene, encoding a putative tyrosine kinase/dual-specificity phosphatase, in a screen for D. discoideum mutants altered in allowing L. pneumophila intracellular replication. Inactivation of dupA resulted in depressed L. pneumophila growth and sustained hyperphosphorylation of the amoebal MAP kinase ERK1, consistent with loss of a phosphatase activity. Bacterial challenge of wild-type amoebae induced dupA expression and resulted in transiently increased ERK1 phosphorylation, suggesting that dupA and ERK1 are part of a response to bacteria. Indeed, over 500 of the genes misregulated in the dupA− mutant were regulated in response to L. pneumophila infection, including some thought to have immune-like functions. MAP kinase phosphatases are known to be highly upregulated in macrophages challenged with L. pneumophila. Thus, DupA may regulate a MAP kinase response to bacteria that is conserved from amoebae to mammals

Analysis and Optimization of GNN-Based Recommender Systems on Persistent Memory

Graph neural networks (GNNs), which have emerged as an effective method for handling machine learning tasks on graphs, bring a new approach to building recommender systems, where the task of recommendation can be formulated as the link prediction problem on user-item bipartite graphs. Training GNN-based recommender systems (GNNRecSys) on large graphs incurs a large memory footprint, easily exceeding the DRAM capacity on a typical server. Existing solutions resort to distributed subgraph training, which is inefficient due to the high cost of dynamically constructing subgraphs and significant redundancy across subgraphs. The emerging persistent memory technologies provide a significantly larger memory capacity than DRAMs at an affordable cost, making single-machine GNNRecSys training feasible, which eliminates the inefficiencies in distributed training. One major concern of using persistent memory devices for GNNRecSys is their relatively low bandwidth compared with DRAMs. This limitation can be particularly detrimental to achieving high performance for GNNRecSys workloads since their dominant compute kernels are sparse and memory access intensive. To understand whether persistent memory is a good fit for GNNRecSys training, we perform an in-depth characterization of GNNRecSys workloads and a comprehensive analysis of their performance on a persistent memory device, namely, Intel Optane. Based on the analysis, we provide guidance on how to configure Optane for GNNRecSys workloads. Furthermore, we present techniques for large-batch training to fully realize the advantages of single-machine GNNRecSys training. Our experiment results show that with the tuned batch size and optimal system configuration, Optane-based single-machine GNNRecSys training outperforms distributed training by a large margin, especially when handling deep GNN models

Cloning and expression trait of UDP-glucose:flavonoid 3-O-glucosyltransferase gene (UF3GT) in turnip

Anthocyanin is a class of important secondary metabolites in plants. UDP-glucose:flavonoid  3-O-glucosyltransferase (UF3GT) is a committed catalytic enzyme in the late stage of anthocyanin  biosynthesis. BrUF3GT1 and BrUF3GT2 genes were cloned by reverse transcription polymerase chain reaction  (RT-PCR) method from ‘Tsuda’ and ‘Yurugi Akamaru’ turnips. The open reading frame (ORF) of BrUF3GT1 and  BrUF3GT2 genes contained 1407 bp encoding proteins of 468 amino acids. Amino acid sequence analysis  showed that BrUF3GT1 and BrUF3GT2 had 87% identity to UF3GT of Arabidopsis thaliana, and the  glycosyltransferase protein family domain was in the amino acids sequence from 16 to 453. The nucleotide  sequence of BrUF3GT1 and BrUF3GT2 genes showed only seven nucleotide differences, and one common  deduced amino acid sequence. The northern blotting results showed that the expression of BrUF3GT1 and BrUF3GT2 genes could be induced by irradiation of ultra-violet A (UV-A), and the expression of the genes was correlated with light-exposure time. The 51.88 and 51.89 KDa proteins of BrUF3GT1 and BrUF3GT2 were  successfully purified after prokaryotic induced expression.Key words: Turnip, UDP-glucose:flavonoid 3-O-glucosyltransferase (UF3GT) gene, gene clone, sequence analysis, gene expression

A target repurposing approach identifies N-myristoyltransferase as a new candidate drug target in filarial nematodes

Myristoylation is a lipid modification involving the addition of a 14-carbon unsaturated fatty acid, myristic acid, to the N-terminal glycine of a subset of proteins, a modification that promotes their binding to cell membranes for varied biological functions. The process is catalyzed by myristoyl-CoA:protein N-myristoyltransferase (NMT), an enzyme which has been validated as a drug target in human cancers, and for infectious diseases caused by fungi, viruses and protozoan parasites. We purified Caenorhabditis elegans and Brugia malayi NMTs as active recombinant proteins and carried out kinetic analyses with their essential fatty acid donor, myristoyl-CoA and peptide substrates. Biochemical and structural analyses both revealed that the nematode enzymes are canonical NMTs, sharing a high degree of conservation with protozoan NMT enzymes. Inhibitory compounds that target NMT in protozoan species inhibited the nematode NMTs with IC50 values of 2.5-10 nM, and were active against B. malayi microfilariae and adult worms at 12.5 µM and 50 µM respectively, and C. elegans (25 µM) in culture. RNA interference and gene deletion in C. elegans further showed that NMT is essential for nematode viability. The effects observed are likely due to disruption of the function of several downstream target proteins. Potential substrates of NMT in B. malayi are predicted using bioinformatic analysis. Our genetic and chemical studies highlight the importance of myristoylation in the synthesis of functional proteins in nematodes and have shown for the first time that NMT is required for viability in parasitic nematodes. These results suggest that targeting NMT could be a valid approach for the development of chemotherapeutic agents against nematode diseases including filariasis

Casting process design and wear properties of a high chromium cast iron hammer

In this article, both chemical composition and structure of a high chromium iron hammer head were designed and analyzed respectively. Also, the casting process was investigated and optimized through numerical simulation using commercial software View Cast. On the basis of numerical simulation and optimization, several hammer heads with fine surface quality and no internal defects were cast into one mold through shell molding and string casting process. In addition, heat treatment of the as-cast hammer head was carried out. Consequently, the microstructure was observed, and wear resistance was tested. After being quenched at the temperature of 950℃ and tempered at the temperature in the range of 230-260℃, the microstructure of the hammer is made up from tempered martensite, retained austenite and network eutectic carbides. The hardness is 60 HRC. Experimental result shows that the wear loss is slowly increased with an increase in load and rotating speed

Towards Fast, Adaptive, and Hardware-Assisted User-Space Scheduling

Modern datacenter applications are prone to high tail latencies since their requests typically follow highly-dispersive distributions. Delivering fast interrupts is essential to reducing tail latency. Prior work has proposed both OS- and system-level solutions to reduce tail latencies for microsecond-scale workloads through better scheduling. Unfortunately, existing approaches like customized dataplane OSes, require significant OS changes, experience scalability limitations, or do not reach the full performance capabilities hardware offers. The emergence of new hardware features like UINTR exposed new opportunities to rethink the design paradigms and abstractions of traditional scheduling systems. We propose LibPreemptible, a preemptive user-level threading library that is flexible, lightweight, and adaptive. LibPreemptible was built with a set of optimizations like LibUtimer for scalability, and deadline-oriented API for flexible policies, time-quantum controller for adaptiveness. Compared to the prior state-of-the-art scheduling system Shinjuku, our system achieves significant tail latency and throughput improvements for various workloads without modifying the kernel. We also demonstrate the flexibility of LibPreemptible across scheduling policies for real applications experiencing varying load levels and characteristics.Comment: Accepted by HPCA202