Advances in the Study of Magnesium Alloys and Their Use in Bone Implant Material

Magnesium and magnesium alloys have great application potential in the field of orthopaedics. Compared with traditional inorganic nonmetallic materials and medical polymer materials, magnesium alloys have many advantages, such as better strength, toughness, fatigue resistance, and easy processing. Its mechanical properties are suitable and controllable. It can meet the same elastic modulus, cell compatibility, and biodegradability as human cortical bone. There are also some drawbacks for biodegradability, as magnesium and its alloys, with their high degradation rate, can cause insufficient integrity of the mechanical properties. This paper summarises the research on magnesium and its magnesium alloy materials in the field of bone implantation, looking at what magnesium and its magnesium alloys are, the history of magnesium alloys in bone implant materials, the manufacturing of magnesium alloys, the mechanical properties of magnesium alloys, the bio-compatibility and clinical applications of magnesium alloys, the shortcomings, and the progress of research in recent years

Synthesis and biological evaluation of novel folic acid receptor-targeted, β-cyclodextrin-based drug complexes for cancer treatment

Drug targeting is an active area of research and nano-scaled drug delivery systems hold tremendous potential for the treatment of neoplasms. In this study, a novel cyclodextrin (CD)-based nanoparticle drug delivery system has been assembled and characterized for the therapy of folate receptor-positive [FR(+)] cancer. Water-soluble folic acid (FA)-conjugated CD carriers (FACDs) were successfully synthesized and their structures were confirmed by 1D/2D nuclear magnetic resonance (NMR), matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS), high performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR), and circular dichroism. Drug complexes of adamatane (Ada) and cytotoxic doxorubicin (Dox) with FACD were readily obtained by mixed solvent precipitation. The average size of FACD-Ada-Dox was 1.5-2.5 nm. The host-guest association constant Ka was 1,639 M-1 as determined by induced circular dichroism and the hydrophilicity of the FACDs was greatly enhanced compared to unmodified CD. Cellular uptake and FR binding competitive experiments demonstrated an efficient and preferentially targeted delivery of Dox into FR-positive tumor cells and a sustained drug release profile was seen in vitro. The delivery of Dox into FR(+) cancer cells via endocytosis was observed by confocal microscopy and drug uptake of the targeted nanoparticles was 8-fold greater than that of non-targeted drug complexes. Our docking results suggest that FA, FACD and FACD-Ada-Dox could bind human hedgehog interacting protein that contains a FR domain. Mouse cardiomyocytes as well as fibroblast treated with FACD-Ada-Dox had significantly lower levels of reactive oxygen species, with increased content of glutathione and glutathione peroxidase activity, indicating a reduced potential for Dox-induced cardiotoxicity. These results indicate that the targeted drug complex possesses high drug association and sustained drug release properties with good biocompatibility and physiological stability. The novel FA-conjugated β-CD based drug complex might be promising as an anti-tumor treatment for FR(+) cancer

In the direction of a sustainable future: A Comprehensive Review of Evolution, Environmental Impacts, and Future Prospects of Bioenergy

As global focus sharpens on carbon emissions and environmental protection; the pursuit of sustainable development permeates every sector. Against the backdrop of increasing fossil fuel prices and relentless energy demand, the exploration of clean energy has become paramount. This paper presents a comprehensive review of bioenergy. It introduces the concept and underscores its importance, tracing the historical stages and accomplishments in its development. The paper explicates different types of bioenergy and their chemical operating principles. The integral system of bioenergy is also evaluated, focusing on crucial components: bioenergy feedstocks, processing technologies, transport process, storage, and grid integration. The paper concludes with an assessment of bioenergy's economic and environmental impacts, considering market dynamics and future prospects, and suggests potential mitigation measures against its environmental repercussions

Research on High Density Water-Based Drilling Fluid of Complete Tectonic for the Southern Edge of the Junggar Basin

The southern edge of the Junggar basin in China has great potential for oil and gas exploitation. However, during drilling in this area, many borehole problems occurred, which leads to low rate of penetration and long drilling cycle. The main reasons from the geological aspect was that this area was affected by tectonic process, which leaded to development of complete tectonic, formation nappe along fault, big dip angles and poor stability. The Formation E2-3a was dominated by mudstone which was sensitive to water and can easily cause shale hydration expansion and dispersal. The instability of the formation is one of the major causes of borehole collapse and tight hole shrinkage. In order to improve the drilling speed and accelerate the process of exploration of southern edge in Juggar basin, the drilling fluids used in this region are studied based on data of geology and drilling data in this paper, Based on the characteristics of the formation E2-3a, a new high density PRT-organic salt drilling fluid is selected by conducting a large number of laboratory tests. Test results of the inhibitory, sealing and stain resistance of this fluid indicates that it could satisfy the drilling requirement in southern edge of the Junggar basin. And field applications also turned out to be positive, the drilling speed was increased by 22% and drilling cycle was reduced by 4 days

MicroRNA-378-3p/5p suppresses the migration and invasiveness of oral squamous carcinoma cells by inhibiting KLK4 expression

Distant metastasis frequently occurs in oral squamous cell carcinoma (OSCC) and contributes to the adverse prognosis of OSCC. However, the potential mechanisms have not been clarified yet. This study aimed to evaluate the role of miR-378 in the migration and invasion of OSCC in vitro and in vivo. According to our results, the migration and invasion abilities were increased in miR-378-overexpressing cells, while decreased in miR-378-3p/5p-silencing cells. In addition, overexpression of miR-378 suppressed the expressions and activities of MMP-9 and MMP-2. Epithelial-mesenchymal transition (EMT) was restrained by overexpression of miR-378 as evidenced by increase in E-cadherin expression and decrease in N-cadherin and uPA expression. However, the miR-378-3p/5p knockdown groups had the opposite results. Moreover, kallikrein-related peptidase 4 (KLK4) was confirmed to be a target gene of miR-378. Overexpression of KLK4 reversed miR-378 overexpression-induced decrease in migration and invasion via upregulating MMP-9, MMP-2, and N-cadherin levels, while downregulating E-cadhrin level. Finally, the number of metastasis nodules in the lung tissues of nude mice was reduced by overexpression of miR-378, whereas the metastasis nodule number was raised by miR-378 knockdown. Taken together, our study suggests that miR-378/KLK4 axis is involved in the mechanisms of the migration and invasion of OSCC cells. Targeting miR-378/KLK4 axis may be an effective measure for treating OSCC.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Advances in the Study of Magnesium Alloys and Their Use in Bone Implant Material

Magnesium and magnesium alloys have great application potential in the field of orthopaedics. Compared with traditional inorganic nonmetallic materials and medical polymer materials, magnesium alloys have many advantages, such as better strength, toughness, fatigue resistance, and easy processing. Its mechanical properties are suitable and controllable. It can meet the same elastic modulus, cell compatibility, and biodegradability as human cortical bone. There are also some drawbacks for biodegradability, as magnesium and its alloys, with their high degradation rate, can cause insufficient integrity of the mechanical properties. This paper summarises the research on magnesium and its magnesium alloy materials in the field of bone implantation, looking at what magnesium and its magnesium alloys are, the history of magnesium alloys in bone implant materials, the manufacturing of magnesium alloys, the mechanical properties of magnesium alloys, the bio-compatibility and clinical applications of magnesium alloys, the shortcomings, and the progress of research in recent years

Determination of intraceullar ROS, GPx and GSH levels in mouse 3T3 cells.

<p>Plot a shows the level of intracellular ROS in 3T3 cells treated with Dox, Ada-Dox or FACD-Ada-Dox at 5.0 µM over 60 min at 37°C in the culture media. Cells were treated with CM-H₂DCFDA. Plot b displays the activity of GPx in 3T3 cells in the presence of Dox, Ada-Dox or FACD-Ada-Dox at 5.0 µM. Plot c shows the GSH concentrations (expressed as nmol/mg protein) in 3T3 cells in the presence of Dox, Ada-Dox or FACD-Ada-Dox at 5.0 µM. Values are the mean ± SD of three different homogenates of cells analyzed in triplicate. ^*<i>P</i><0.05; ^**<i>P</i><0.01; and ^***<i>P</i><0.001.</p