Gammaproteobacteria, a core taxon in the guts of soil fauna, are potential responders to environmental concentrations of soil pollutants

Background: The ubiquitous gut microbiotas acquired from the environment contribute to host health. The gut microbiotas of soil invertebrates are gradually assembled from the microecological region of the soil ecosystem which they inhabit, but little is known about their characteristics when the hosts are under environmental stress. The rapid development of high-throughput DNA sequencing in the last decade has provided unprecedented insights and opportunities to characterize the gut microbiotas of soil invertebrates. Here, we characterized the core, transient, and rare bacterial taxa in the guts of soil invertebrates using the core index (CI) and developed a new theory of global microbial diversity of soil ecological microregions. Results: We found that the Gammaproteobacteria could respond indiscriminately to the exposure to environmental concentrations of soil pollutants and were closely associated with the physiology and function of the host. Meanwhile, machine-learning models based on metadata calculated that Gammaproteobacteria were the core bacteria with the highest colonization potential in the gut, and further identified that they were the best indicator taxon of the response to environmental concentrations of soil pollution. Gammaproteobacteria also closely correlated with the abundance of antibiotic resistance genes. Conclusions: Our results determined that Gammaproteobacteria were an indicator taxon in the guts of the soil invertebrates that responded to environmental concentrations of soil pollutants, thus providing an effective theoretical basis for subsequent assessments of soil ecological risk. The results of the physiological and biochemical analyses of the host and the microbial-community functions, and the antibiotic resistance of Gammaproteobacteria, provide new insights for evaluating global soil ecological health

Studies on Chemical Characterization of Ginkgo Amillaria Oral Solution and Its Drug–Drug Interaction With Piceatannol 3′-O-β-D-Glucopyranoside for Injection

Ginkgo Amillaria oral solution (GAO) is commonly used for the treatment of cardiovascular and cerebrovascular diseases in China. Piceatannol-3′-O-β-D-glucopyranoside for injection (PGI) is mainly used for the prevention and treatment of ischemic cerebrovascular diseases. With the spread of cerebrovascular disease, the possibility of combining the two drugs has increased; however, there is no research on the drug–drug interaction (DDI) between these two medicines. In this paper, an ultrahigh-performance liquid chromatography/quadrupole–orbitrap mass spectrometry (UHPLC/Q-Orbitrap MS) method was established to characterize the chemical constituents of GAO first; 62 compounds were identified or tentatively identified based on their retention time (RT), MS, and MS/MS data. Nine main compounds were determined by ultrahigh-performance liquid chromatography/triple quadrupole mass spectrometry (UPLC-QQQ-MS). Furthermore, incubation with liver microsomes in vitro was fulfilled; the results showed that GAO had a significant inhibitory effect on UGT1A9 and UGT2B7 (p < 0.05), and PGI was mainly metabolized by UGT1A9. The identification results of in vivo metabolites of PGI showed that PGI mainly undergoes a phase II binding reaction mediated by UDP-glucuronosyltransferase (UGT) and sulfotransferase (SULT) in vivo. Therefore, pharmacokinetic studies were performed to investigate the DDI between GAO and PGI. The results showed that the AUC (p < 0.05) and T1/2 (p < 0.05) of PGI in vivo were significantly increased when administered together with GAO, whereas the CL was significantly decreased (p < 0.05). The exploration of in vitro and in vivo experiments showed that there was a DDI between GAO and PGI

Hydrochemical Characteristics and Quality Assessment of Groundwater under the Impact of Seawater Intrusion and Anthropogenic Activity in the Coastal Areas of Zhejiang and Fujian Provinces, China

AbstractCoastal groundwater is an important resource in the developed region associated with human health and sustainable economic development. To identify the origins of salinity and evaluate the impact of water-rock interactions, seawater intrusion (SWI), and evaporation on groundwater in the coastal areas of Zhejiang and Fujian provinces, a comprehensive investigation was performed. Meanwhile, nitrate and fluoride indicators resulting from the anthropogenic activity and SWI were also considered. At last, the water quality index (WQI) of coastal groundwater was evaluated with geochemical and multivariate statistical methods. The results indicated that (1) the groundwater in coastal areas of Zhejiang and Fujian provinces has been affected by SWI to varying degrees. The analysis of selected ion ratios (Na+/Cl− and Br−/Cl−) and isotopic compositions showed that SWI is the predominant cause of increasing salinity in the groundwater of Zhejiang Province, while the cause is water-rock interactions (ion exchange and mineral weathering) in Fujian Province. The hydrochemical evolution path of groundwater in Zhejiang Province is Ca/Mg-HCO3 to Na-Cl, while a different pattern of Ca/Mg-HCO3 to Na (Mg/Ca)-Cl occurs in Fujian Province. However, the trend of SWI development in both provinces was freshening. (2) Nitrification, sewage infiltration, and SWI increased the NO3− content in groundwater. Some of the NO3− concentration in Fujian Province exceeds the standard, and the nitrogen pollution was more serious than in Zhejiang Province. The F− content in coastal groundwater was affected by SWI and mineral dissolution; the F− content in Zhejiang Province was higher than in Fujian Province, which was close to the groundwater standard limit. The average WQI value of Zhejiang was 103.61, and the WQI of Fujian was 61.69, indicating that the coastal groundwater quality in Fujian Province was better than in Zhejiang Province. The results of the study revealed the impact of SWI and anthropogenic activity on groundwater in the southern coastal zone of China and will be valuable for sustainable groundwater resource management

Negative effects of abamectin on soil microbial communities in the short term

With the widespread use of abamectin in agriculture, there is increasing urgency to assess the effects of abamectin on soil microorganisms. Here, we treated plant–soil microcosms with abamectin at concentrations of 0.1 and 1.0 mg/kg and quantified the impacts of abamectin on bulk and rhizosphere soil microbial communities by shotgun metagenomics after 7 and 21 days of exposure. Although abamectin was reported to be easily degradable, it altered the composition of the soil microbial communities, disrupted microbial interactions, and decreased community complexity and stability after 7 days of exposure. After treatment with abamectin at a concentration of 1.0 mg/kg, some opportunistic human diseases, and soil-borne pathogens like Ralstonia were enriched in the soil. However, most ecological functions in soil, particularly the metabolic capacities of microorganisms, recovered within 21 days after abamectin treatment. The horizontal and vertical gene transfer under abamectin treatments increased the levels of antibiotic resistance genes dissemination. Overall, our findings demonstrated the negative effects of abamectin on soil ecosystems in the short-term and highlight a possible long-term risk to public and soil ecosystem health associated with antibiotic resistance genes dissemination

Optimization for the Production of Surfactin with a New Synergistic Antifungal Activity

-surfactin and the optimization of its production by the response surface method.O. A production of 134.2 mg/L, which were in agreement with the prediction, was observed in a verification experiment. In comparison to the production of original level (88.6 mg/L), a 1.52-fold increase had been obtained.-surfactin

Spatiotemporal Regulation of Rho GTPases in Neuronal Migration

Neuronal migration is essential for the orchestration of brain development and involves several contiguous steps: interkinetic nuclear movement (INM), multipolar–bipolar transition, locomotion, and translocation. Growing evidence suggests that Rho GTPases, including RhoA, Rac, Cdc42, and the atypical Rnd members, play critical roles in neuronal migration by regulating both actin and microtubule cytoskeletal components. This review focuses on the spatiotemporal-specific regulation of Rho GTPases as well as their regulators and effectors in distinct steps during the neuronal migration process. Their roles in bridging extracellular signals and cytoskeletal dynamics to provide optimal structural support to the migrating neurons will also be discussed

High-Precision SLAM Based on the Tight Coupling of Dual Lidar Inertial Odometry for Multi-Scene Applications

Simultaneous Localization and Mapping (SLAM) is an essential feature in many applications of mobile vehicles. To solve the problem of poor positioning accuracy, single use of mapping scene, and unclear structural characteristics in indoor and outdoor SLAM, a new framework of tight coupling of dual lidar inertial odometry is proposed in this paper. Firstly, through external calibration and an adaptive timestamp synchronization algorithm, the horizontal and vertical lidar data are fused, which compensates for the narrow vertical field of view (FOV) of the lidar and makes the characteristics of vertical direction more complete in the mapping process. Secondly, the dual lidar data is tightly coupled with an Inertial Measurement Unit (IMU) to eliminate the motion distortion of the dual lidar odometry. Then, the value of the lidar odometry after correcting distortion and the pre-integrated value of IMU are used as constraints to establish a non-linear least-squares objective function. Joint optimization is then performed to obtain the best value of the IMU state values, which will be used to predict the state of IMU at the next time step. Finally, experimental results are presented to verify the effectiveness of the proposed method

Growth, Structure and Optical Characterization of Rb3Ti3P5O20 Single Crystal

Phosphate crystals attract much attention on account of their rich crystal structures and excellent physical and chemical properties. Herein, Rb3Ti3P5O20 single crystals were grown by the high temperature solution method using Rb2CO3 and NH4H2PO4 as the fluxes. This crystal, with non-centrosymmetric Pca21 space group, presents a three-dimensional framework structure composed of [TiO6] octahedron, [PO4] tetrahedra, and [P2O7] dimers. The electronic structure was measured via X-ray photoelectron spectroscopy. The measurements found that Rb3Ti3P5O20 has stronger Ti–O ionic bonding properties and weaker P–O covalent bonding properties compared to RbTiOPO4. Optical measurements indicated that Rb3Ti3P5O20 has a 3.54 eV band gap and a wide transmission range (0.33–4.5 μm). Theoretical calculations showed that Rb3Ti3P5O20 crystals have a moderate birefringence of 0.079 at 1064 nm. In addition, the relationship of the structure–property was studied using first-principles method. The results demonstrated that TiO6 octahedron played a significant role for the optical properties

An Energy-Efficient BJT-Based Temperature Sensor with ±0.8 °C (3<i>σ</i>) Inaccuracy from −50 to 150 °C

This article presents an energy-efficient BJT-based temperature sensor. The output of sensing front-ends is modulated by employing an incremental Δ-Σ ADC as a readout interface. The cascoded floating-inverter-based dynamic amplifier (FIA) is used as the integrator instead of the conventional operational transconductance amplifier (OTA) to achieve a low power consumption. To enhance the accuracy, chopping and dynamic element matching (DEM) are applied to eliminate the component mismatch error while β-compensation resistor and optimized bias current are used to minimize the effect of β variation. Fabricated in a standard 180-nm CMOS process, this sensor has an active area of 0.13 mm2. While dissipating only 45.7 μW in total, the sensor achieves an inaccuracy of ±0.8 °C (3σ) from −50 °C to 150 °C after one-point calibration

A Comprehensive Evaluation Model for Local Summer Climate Suitability under Global Warming: A Case Study in Zhejiang Province

In the context of global warming, how to measure summer climate suitability at a local scale is important for meteorological services. Considering meteorological and ecological conditions, body comfort, and the atmospheric environment, an assessment method for summer climate suitability for Zhejiang Province is proposed. In this paper, a summer suitable index (SSI) for Zhejiang is calculated, including four secondary indices: a summer cool index (SCI), a comfort days index (CDI), a good air days index (GADI) and a vegetation cover index (VCI). Using a local evaluation criterion, summer climate suitable areas are distinguished objectively according to the SSI. The results show that especially suitable regions account for 4.97% of Zhejiang Province, very suitable regions account for 22.2%, suitable regions account for 39.58%, and general regions account for 33.25%. The summer climate suitable areas are located mainly in high mountains and hills and coastal island areas while plain areas cannot be considered a suitable destination for summer tourism. By comparison and discussion, the SSI is demonstrated to capture summer climate suitability well. In contrast to a fixed evaluation index, benchmark values obtained for the SSI depend on the local climate and the index is straightforward to apply