175 research outputs found

    On solutions of matrix equation AXB + CYD = F

    Get PDF
    AbstractIn this paper, the matrix equation with two unknown matrices X, Y of form AXB + CYD = F is discussed. By applying the canonical correlation decomposition (CCD) of matrix pairs, we obtain expressions of the least-squares solutions of the matrix equation, and sufficient and necessary conditions for the existence and uniqueness of the solutions. We also derive a general form of the solutions. We also study the least-squares Hermitian (skew-Hermitian) solutions of equation AXAH + CYCH = F

    Compositional identification and authentication of Chinese honeys by 1 H NMR combined with multivariate analysis

    Get PDF
    Abstract(#br)Honey authentication has been becoming more and more important and necessary to the honey producers, the consumers and the market regulatory authority due to its favorite organoleptic and healthy properties, high value and increasing export but prevalent falsification practice for economic motivation in China and the potential health risk of adulterated honey. In this study, we obtained the spectral profiles of 90 authentic and 75 adulterated Chinese honey samples by means of high resolution nuclear magnetic resonance (NMR) spectroscopy, and 65 kinds of major and minor components in honey were identified and quantified from their NMR spectra. Combining with the multivariate statistical analyses including principal component analysis (PCA), linear discriminant analysis (LDA), and orthogonal partial least squared-discriminant analysis (OPLS-DA), the discrimination models were successfully established to identify the adulterated honeys from the authentic ones with an accurate rate of 97.6%. Furthermore, the corresponding volcano plot was used to screen out 8 components including proline, xylobiose, uridine, β-glucose, melezitose, turanose, lysine and an unknown component, which are responsible for the differentiation between the authentic and adulterated honeys and will help to control Chinese domestic honey market

    Development of New Microsatellite DNA Markers from Apostichopus japonicus and Their Cross-Species Application in Parastichopus parvimensis and Pathallus mollis

    Get PDF
    Twenty microsatellite DNA markers were developed for sea cucumber and used to investigate polymorphisms of 60 wild Apostichopus japonicus individuals collected from China. It revealed that all the markers were polymorphic. A total of 164 alleles were detected at 20 loci. The number of alleles per locus varied from 3 to 17 with an average of 8.2, and the expected heterozygosities of each locus ranged from 0.03 to 0.89 with an average of 0.64. Cross-species amplification was also conducted in Parastichopus parvimensis collected from the United States and Pathallus mollis collected from Peru. The result showed that 17 loci amplified Parastichopus parvimensis DNAs while only 4 loci could amplify Pathallus mollis DNAs. All of the polymorphic markers would be useful for future genetic breeding and the assessment of genetic variation within sea cucumbers

    Time Course Transcriptomic Study Reveals the Gene Regulation During Liver Development and the Correlation With Abdominal Fat Weight in Chicken

    Get PDF
    Background: The liver is the central metabolic organ of animals. In chicken, knowledge on the relationship between gene expression in the liver and fat deposition during development is still limited. A time-course transcriptomic study from the embryonic (day 12) to the egg-producing period (day 180 after hatch) was performed to profile slow-growing meat type chicken liver gene expression and to investigate its correlation with abdominal fat deposition.Results: The transcriptome profiles showed a separation of the different developmental stages. In total, 13,096 genes were ubiquitously expressed at all the tested developmental stages. The analysis of differentially expressed genes between adjacent developmental stages showed that biosynthesis of unsaturated fatty acids pathway was enriched from day 21 to day 140 after hatch. The correlation between liver gene expression and the trait abdominal fat weight (AFW) was analyzed by weighted gene co-expression network analysis. The genes MFGE8, HHLA1, CKAP2, and ACSBG2 were identified as hub genes in AFW positively correlated modules, which suggested important roles of these genes in the lipid metabolism in chicken liver.Conclusion: Our results provided a resource of developmental transcriptome profiles in chicken liver and suggested that the gene ACSBG2 among other detected genes can be used as a candidate gene for selecting low AFW chickens

    A recombinant avian antibody against VP2 of infectious bursal disease virus protects chicken from viral infection

    Get PDF
    【Abstract】A stable cell-line was established that expressed the recombinant avian antibody (rAb) against the infectious bursal disease virus (IBDV). rAb exhibited neutralization activity to IBDV-B87 strain in DF1 cells. The minimum rAb concentration required for inhibition of the cytopathic effect (CPE) was 1.563 μg/mL. To test the efficacy of rAb, a 168-h cohabitation challenge experiment was performed to transmit the disease from the chickens challenged with vvIBDV (HLJ0504 strain) to three test groups of chickens, i.e. (1) chickens treated with rAb, (2) chickens treated with yolk antibody, and (3) non-treatment chickens. The survival rates of chickens treated with rAb, yolk antibody and without treatment were 73%, 67% and 20%, respectively. Another batch of chickens was challenged with IBDV (BC6/85 strain) and then injected with rAb (1.0 mg/kg) 6, 24 and 36 h post-challenge. Non-treatment chickens had 100% morbidity, whereas those administered with rAb exhibited only 20% morbidity. Morbidity was evaluated using clinical indicators and bursal histopathological section. This study provides a new approach to treating IBDV and the rAb represents a promising candidate for this IBDV therapy.This research was supported by Heilongjiang province project of applied technology research and development (2013GC13C105) and The National Natural Science Fund biologic science base improve program of research training and capacity (J1210069/J0124)
    corecore