Spatiotemporal coupled-mode equations for arbitrary pulse transformation

Spatiotemporal modulation offers a variety of opportunities for light manipulations. In this paper, we propose a way towards arbitrary transformation for pulses sequentially propagating within one waveguide in space via temporal waveguide coupling. The temporal waveguide coupling operation is achieved by spatiotemporally modulating the refractive index of the spatial waveguide with a traveling wave through segmented electrodes. We derive the temporal coupled-mode equations and discuss how systematic parameters affect the temporal coupling coefficients. We further demonstrated a temporal Mach-Zehnder interferometer and universal multiport interferometer, which enables arbitrary unitary transformation for pulses. We showcase a universal approach for transforming pulses among coupled temporal waveguides, which requires only one spatial waveguide under spatiotemporal modulation, and hence provide a flexible, compact, and highly compatible method for optical signal processing in time domain

Characterization of cell cycle and apoptosis using flow cytometry for bioprocess monitoring

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Color 3D Printing: Theory, Method, and Application

Our research team proposes a colored manufacturing technology with a layer-by-layer printing process. Using digital inkjet printing in layer-by-layer printing color graphics, a further low-cost color 3D Printing (3DP) technology can be developed. It can provide an integrated way to prototype and reproduce 3D objects, from concept to design and manufacturing. Ultimately, with fast graphics printing method, it guarantees a feasible way to further promote cultural and creative products

H-beta Line Width and the UV-X-ray Spectra of Luminous AGN

The width of the broad H-beta emission line is the primary defining characteristic of the NLS1 class. This parameter is also an important component of Boroson and Green's optical Eigenvector 1 (EV1), which links steeper soft X-ray spectra with narrower H-beta emission, stronger H-beta blue wing, stronger optical Fe II emission, and weaker [O III] lambda 5007. Potentially, EV1 represents a fundamental physical process linking the dynamics of fueling and outflow with the accretion rate. We attempted to understand these relationships by extending the optical spectra into the UV for a sample of 22 QSOs with high quality soft-X-ray spectra, and discovered a whole new set of UV relationships that suggest that high accretion rates are linked to dense gas and perhaps nuclear starbursts. While it has been argued that narrow (BLR) H-beta means low Black Hole mass in luminous NLS1s, the C IV, lambda 1549 and Ly alpha emission lines are broader, perhaps the result of outflows driven by their high Eddington accretion rates. We present some new trends of optical-UV with X-ray spectral energy distributions. Steeper X-ray spectra appear associated with stronger UV relative to optical continua, but the presence of strong UV absorption lines is associated with depressed soft X-rays and redder optical-UV continua.Comment: Invited talk presented at the Joint MPE,AIP,ESO workshop on NLS1s, Bad Honnef, Dec. 1999, to appear in New Astronomy Reviews; also available at http://wave.xray.mpe.mpg.de/conferences/nls1-worksho

Albiflorin attenuates inflammation and apoptosis by upregulating AMPK-mediated expression of CDX2 in a mouse model of ulcerative colitis

Purpose: To investigate the mechanism underlying the ameliorative effect of albiflorin (AF) on ulcerative colitis (UC) in dextran sulphate sodium (DSS)-induced mice model. Method: Female C57BL/6 mice were administered DSS to establish a mice model of UC. After one week, the mice received AF, and the body weight and length of colon were measured. The histopathological features of colon tissues treated with hematoxylin-eosin (H & E) stain were examinedby microscopy. Expression of inflammatory cytokines and apoptosis-related proteins were determined using enzyme-linked immunosorbent assay (ELISA) and western blotting. Results: The relative abundance of goblet cells and crypts of mice were significantly reduced in DSSinduced UC mice model; furthermore, focal ulcers and mucosal damage were apparent. Moreover, treatment with DSS decreased body weight and colon length, downregulated Bcl-2 and AMPK pathwayrelated proteins, increased inflammatory cytokines levels, and upregulated Bax and cleaved caspase-3. In contrast, treatment with AF completely ameliorated DSS-induced effects. Conclusion: AF treatment attenuated DSS-induced inflammation response and apoptosis via AMPK pathway and modulation of CDX2 expression in UC mice model. Keyword: Albiflorin, Ulcerative colitis, AMPK, CDX2, Apoptosi

Linking Incomplete Reprogramming to the Improved Pluripotency of Murine Embryonal Carcinoma Cell-Derived Pluripotent Stem Cells

Somatic cell nuclear transfer (SCNT) has been proved capable of reprogramming various differentiated somatic cells into pluripotent stem cells. Recently, induced pluripotent stem cells (iPS) have been successfully derived from mouse and human somatic cells by the over-expression of a combination of transcription factors. However, the molecular mechanisms underlying the reprogramming mediated by either the SCNT or iPS approach are poorly understood. Increasing evidence indicates that many tumor pathways play roles in the derivation of iPS cells. Embryonal carcinoma (EC) cells have the characteristics of both stem cells and cancer cells and thus they might be the better candidates for elucidating the details of the reprogramming process. Although previous studies indicate that EC cells cannot be reprogrammed into real pluripotent stem cells, the reasons for this remain unclear. Here, nuclei from mouse EC cells (P19) were transplanted into enucleated oocytes and pluripotent stem cells (P19 NTES cells) were subsequently established. Interestingly, P19 NTES cells prolonged the development of tetraploid aggregated embryos compared to EC cells alone. More importantly, we found that the expression recovery of the imprinted H19 gene was dependent on the methylation state in the differential methylation region (DMR). The induction of Nanog expression, however, was independent of the promoter region DNA methylation state in P19 NTES cells. A whole-genome transcriptome analysis further demonstrated that P19 NTES cells were indeed the intermediates between P19 cells and ES cells and many interesting genes were uncovered that may be responsible for the failed reprogramming of P19 cells. To our knowledge, for the first time, we linked incomplete reprogramming to the improved pluripotency of EC cell-derived pluripotent stem cells. The candidate genes we discovered may be useful not only for understanding the mechanisms of reprogramming, but also for deciphering the transition between tumorigenesis and pluripotency

A comparative study on microstructure and properties of traditional laser cladding and high-speed laser cladding of Ni45 alloy coatings

High-speed laser cladding technology can significantly improve the efficiency of coating preparation and effectively widen the application range of laser cladding. In this study, the Ni45 powders were deposited on steel substrate by traditional low speed laser cladding and high-speed laser cladding process, respectively. The cladding efficiency, surface forming, cross-sectional microstructure, microhardness, wear and corrosion resistance properties of the traditional and high-speed laser cladded Ni45 alloy coatings were compared. It can be seen that the thickness of the high-speed laser cladding coating was much thinner than that of the traditional laser cladding coating. Compared with traditional laser cladding, high-speed laser cladding could achieve a cladding speed of 76.86 m/min and a cladding efficiency of 156.79 cm2/min. The microstructure of the two kinds of coatings shows the same growth law, but the microstructure in high-speed laser cladding was smaller and denser, and the columnar crystal interval was narrower, only about 6 μm. It is found that the cooling rate of the traditional laser cladding coating was smaller than that of the high-speed laser cladding, and as the cladding speed increased, the cooling rate became higher and higher. The cross-section microhardness of the traditional laser cladding coating was relatively uniform of 337 HV0.2, while the microhardness of high-speed laser cladding surface increased to about 543 HV0.2. In addition, the wear and corrosion resistance of high-speed laser cladded coatings were better than that of traditional laser cladded coatings. As the cladding speed increased, the wear and corrosion resistance of the cladded coatings became better