Spatiotemporal Tradeoffs and Synergies in Vegetation Vitality and Poverty Transition in Rocky Desertification Area

Vegetation recovery and poverty alleviation are critical problems in the karst national designed poor counties (NPDC) in southwest China. However, little information is available about the relationship between poverty and vegetation dynamics in these areas. In this study, we used remote sensing and statistical datasets from 2000 to 2015 to identify the relations between vegetation dynamics and poverty among the NPDC in southwest rocky desertification areas. We estimated the vegetation dynamics using the Normalized Difference Vegetation Index and poverty with the rural per capita net income. Local indicator of spatial association and the space-time transition type of poverty were applied to identify spatial patterns of the poverty spatial distribution relationship and transition. Also, poverty, natural and ecological governance factorswere assessed using the Geodetector method to uncover the driving factors of karst vegetation. The results showed that vegetation increased significantly (p < 0.05) in karst NPDC (82.82%) and rocky desertification control counties (78.77%). The karst NPDC was significantly clustered. The hot spots of rural per capita net income changed from west and north (2000) to only north (2015) and cold spots changed from east and south (2000) to only south (2015). The rural per capita net income spatiotemporal transitionwas higher in 2000 than in 2015.Wefound aweak synergy between vegetation change and poverty type transition in 42.86% of the browning counties, 45.45% in the slowly greening counties, and 43.65% in stable greening counties. However, 57.50% of counties in the quick greening counties showed a tradeoff relationship with the poverty type transition. The rocky desertification rate and ecological engineering measures affected vegetation dynamics importantly. The results will help decisionmakers to understand the interdependence between vegetation and poverty. This will contribute to better policies formulation to tackle poverty in the karst rocky desertification area

Recruitment and metabolomics between Canna indica and rhizosphere bacteria under Cr stress

It is of positive significance to explore the mechanism of antioxidant and metabolic response of Canna indica under Cr stress mediated by rhizosphere niche. However, the mechanisms of recruitment and interaction of rhizosphere microorganisms in plants still need to be fully understood. This study combined physiology, microbiology, and metabolomics, revealing the interaction between C. indica and rhizosphere microorganisms under Cr stress. The results showed that Cr stress increased the content of malondialdehyde (MDA) and oxygen-free radicals (ROS) in plants. At the same time, the activities of antioxidant enzymes (SOD, POD, and APX) and the contents of glutathione (GSH) and soluble sugar were increased. In addition, Cr stress decreased the α diversity index of C. indica rhizosphere bacterial community and changed its community structure. The dominant bacteria, namely, Actinobacteriota, Proteobacteria, and Chloroflexi accounted for 75.16% of the total sequence. At the same time, with the extension of stress time, the colonization amount of rhizosphere-dominant bacteria increased significantly, and the metabolites secreted by roots were associated with the formation characteristics of Proteobacteria, Actinobacteria, Bacteroidetes, and other specific bacteria. Five critical metabolic pathways were identified by metabolome analysis, involving 79 differentially expressed metabolites, which were divided into 15 categories, mainly including lipids, terpenoids, and flavonoids. In conclusion, this study revealed the recruitment and interaction response mechanism between C. indica and rhizosphere bacteria under Cr stress through multi-omics methods, providing the theoretical basis for the remediation of Cr-contaminated soil

Differential microRNA expression between shoots and rhizomes in Oryza longistaminata using high-throughput RNA sequencing

AbstractPlant microRNAs (miRNAs) play important roles in biological processes such as development and stress responses. Although the diverse functions of miRNAs in model organisms have been well studied, their function in wild rice is poorly understood. In this study, high-throughput small RNA sequencing was performed to characterize tissue-specific transcriptomes in Oryza longistaminata. A total of 603 miRNAs, 380 known rice miRNAs, 72 conserved plant miRNAs, and 151 predicted novel miRNAs were identified as being expressed in aerial shoots and rhizomes. Additionally, 99 and 79 miRNAs were expressed exclusively or differentially, respectively, in the two tissues, and 144 potential targets were predicted for the differentially expressed miRNAs in the rhizomes. Functional annotation of these targets suggested that transcription factors, including squamosa promoter binding proteins and auxin response factors, function in rhizome growth and development. The expression levels of several miRNAs and target genes in the rhizomes were quantified by RT-PCR, and the results indicated the existence of complex regulatory mechanisms between the miRNAs and their targets. Eight target cleavage sites were verified by RNA ligase-mediated rapid 5′ end amplification. These results provide valuable information on the composition, expression and function of miRNAs in O. longistaminata, and will aid in understanding the molecular mechanisms of rhizome development

The first case of pulmonary disease caused by Mycobacterium septicum in China

SummaryMycobacterium septicum is a rapidly growing Mycobacterium (RGM) that rarely causes pulmonary disease globally. We describe a case of M. septicum pulmonary disease, which to our knowledge is the first reported in China. The isolates were identified as M. septicum and were susceptible in vitro to amikacin, ciprofloxacin, ofloxacin, levofloxacin, kanamycin, and sulfamethoxazole

QTL mapping and candidate gene analysis of ferrous iron and zinc toxicity tolerance at seedling stage in rice by genome-wide association study

Background:Ferrous iron (Fe) and zinc (Zn) at high concentration in the soil cause heavy metal toxicity andgreatly affect rice yield and quality. To improve rice production, understanding the genetic and molecularresistance mechanisms to excess Fe and Zn in rice is essential. Genome-wide association study (GWAS) is aneffective way to identify loci and favorable alleles governing Fe and Zn toxicty as well as dissect the geneticrelationship between them in a genetically diverse population.Results:A total of 29 and 31 putative QTL affecting shoot height (SH), root length (RL), shoot fresh weight (SFW),shoot dry weight (SDW), root dry weight (RDW), shoot water content (SWC) and shoot ion concentrations (SFe orSZn) were identified at seedling stage in Fe and Zn experiments, respectively. Five toxicity tolerance QTL (qSdw3a,qSdw3b,qSdw12andqSFe5/qSZn5) were detected in the same genomic regions under the two stress conditionsand 22 candidate genes for 10 important QTL regions were also determined by haplotype analyses.Conclusion:Rice plants share partial genetic overlaps of Fe and Zn toxicity tolerance at seedling stage. Candidategenes putatively affecting Fe and Zn toxicity tolerance identified in this study provide valuable information forfuture functional characterization and improvement of rice tolerance to Fe and Zn toxicity by marker-assistedselection or designed QTL pyramiding

Identification of rhizome-specific genes by genome-wide differential expression Analysis in Oryza longistaminata

<p>Abstract</p> <p>Background</p> <p>Rhizomatousness is a key component of perenniality of many grasses that contribute to competitiveness and invasiveness of many noxious grass weeds, but can potentially be used to develop perennial cereal crops for sustainable farmers in hilly areas of tropical Asia. <it>Oryza longistaminata</it>, a perennial wild rice with strong rhizomes, has been used as the model species for genetic and molecular dissection of rhizome development and in breeding efforts to transfer rhizome-related traits into annual rice species. In this study, an effort was taken to get insights into the genes and molecular mechanisms underlying the rhizomatous trait in <it>O. longistaminata </it>by comparative analysis of the genome-wide tissue-specific gene expression patterns of five different tissues of <it>O. longistaminata </it>using the Affymetrix GeneChip Rice Genome Array.</p> <p>Results</p> <p>A total of 2,566 tissue-specific genes were identified in five different tissues of <it>O. longistaminata</it>, including 58 and 61 unique genes that were specifically expressed in the rhizome tips (RT) and internodes (RI), respectively. In addition, 162 genes were up-regulated and 261 genes were down-regulated in RT compared to the shoot tips. Six distinct <it>cis</it>-regulatory elements (CGACG, GCCGCC, GAGAC, AACGG, CATGCA, and TAAAG) were found to be significantly more abundant in the promoter regions of genes differentially expressed in RT than in the promoter regions of genes uniformly expressed in all other tissues. Many of the RT and/or RI specifically or differentially expressed genes were located in the QTL regions associated with rhizome expression, rhizome abundance and rhizome growth-related traits in <it>O. longistaminata </it>and thus are good candidate genes for these QTLs.</p> <p>Conclusion</p> <p>The initiation and development of the rhizomatous trait in <it>O. longistaminata </it>are controlled by very complex gene networks involving several plant hormones and regulatory genes, different members of gene families showing tissue specificity and their regulated pathways. Auxin/IAA appears to act as a negative regulator in rhizome development, while GA acts as the activator in rhizome development. Co-localization of the genes specifically expressed in rhizome tips and rhizome internodes with the QTLs for rhizome traits identified a large set of candidate genes for rhizome initiation and development in rice for further confirmation.</p

A novel pathogenic species of genus Stenotrophomonas: Stenotrophomonas pigmentata sp. nov

IntroductionStenotrophomonas is a prominent genus owing to its dual nature. Species of this genus have many applications in industry and agriculture as plant growth-promoting rhizobacteria and microbial biological control agents, whereas species such as Stenotrophomonas maltophilia are considered one of the leading gram-negative multi-drug-resistant bacterial pathogens because of their high contribution to the increase in crude mortality and significant clinical challenge. Pathogenic Stenotrophomonas species and most clinical isolates belong to the Stenotrophomonas maltophilia complex (SMc). However, a strain highly homologous to S. terrae was isolated from a patient with pulmonary tuberculosis (TB), which aroused our interest, as S. terrae belongs to a relatively distant clade from SMc and there have been no human association reports.MethodsThe pathogenicity, immunological and biochemical characteristics of 610A2T were systematically evaluated.Results610A2T is a new species of genus Stenotrophomonas, which is named as Stenotrophomonas pigmentata sp. nov. for its obvious brown water-soluble pigment. 610A2T is pathogenic and caused significant weight loss, pulmonary congestion, and blood transmission in mice because it has multiple virulence factors, haemolysis, and strong biofilm formation abilities. In addition, the cytokine response induced by this strain was similar to that observed in patients with TB, and the strain was resistant to half of the anti-TB drugs.ConclusionsThe pathogenicity of 610A2T may not be weaker than that of S. maltophilia. Its isolation extended the opportunistic pathogenic species to all 3 major clades of the genus Stenotrophomonas, indicating that the clinical importance of species of Stenotrophomonas other than S. maltophilia and potential risks to biological safety associated with the use of Stenotrophomonas require more attention