8,773 research outputs found
Thermal Anomalies Detect Critical Global Land Surface Changes
Measurements that link surface conditions and climate can provide critical information on important biospheric changes occurring in the Earth system. As the direct driving force of energy and water fluxes at the surface–atmosphere interface, land surface temperature (LST) provides information on physical processes of land-cover change and energy-balance changes that air temperature cannot provide. Annual maximum LST (LSTmax) is especially powerful at minimizing synoptic and seasonal variability and highlighting changes associated with extreme climatic events and significant land-cover changes. The authors investigate whether maximum thermal anomalies from satellite observations could detect heat waves and droughts, a melting cryosphere, and disturbances in the tropical forest from 2003 to 2014. The 1-km2 LSTmax anomalies peaked in 2010 when 20% of the global land area experienced anomalies of greater than 1 standard deviation and over 4% of the global land area was subject to positive anomalies exceeding 2 standard deviations. Positive LSTmax anomalies display complex spatial patterns associated with heat waves and droughts across the global land area. The findings presented herein show that entire biomes are experiencing shifts in their LSTmax distributions driven by extreme climatic events and large-scale land surface changes, such as melting of ice sheets, severe droughts, and the incremental effects of forest loss in tropical forests. As climate warming and land-cover changes continue, it is likely that Earth’s maximum surface temperatures will experience greater and more frequent directional shifts, increasing the possibility that critical thresholds in Earth’s ecosystems and climate system will be surpassed, resulting in profound and irreversible changes
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P16-12. Relative Dominance of Gag-Specific Cytotoxic T Lymphocytes Is Associated with Viral Load Inversely in HIV-1 Clade B' Infected Chinese
SV‑HotSpot: Detection and visualization of hotspots targeted by structural variants associated with gene expression
A thin layer angiogenesis assay: a modified basement matrix assay for assessment of endothelial cell differentiation
BACKGROUND: Basement matrices such as Matrigel™ and Geltrex™ are used in a variety of cell culture assays of anchorage-dependent differentiation including endothelial cell tube formation assays. The volumes of matrix recommended for these assays (approximately 150 μl/cm(2)) are costly, limit working distances for microscopy, and require cell detachment for subsequent molecular analysis. Here we describe the development and validation of a thin-layer angiogenesis (TLA) assay for assessing the angiogenic potential of endothelial cells that overcomes these limitations. RESULTS: Geltrex™ basement matrix at 5 μl/cm(2) in 24-well (10 μl) or 96-well (2 μl) plates supports endothelial cell differentiation into tube-like structures in a comparable manner to the standard larger volumes of matrix. Since working distances are reduced, high-resolution single cell microscopy, including DIC and confocal imaging, can be used readily. Using MitoTracker dye we now demonstrate, for the first time, live mitochondrial dynamics and visualise the 3-dimensional network of mitochondria present in differentiated endothelial cells. Using a standard commercial total RNA extraction kit (Qiagen) we also show direct RNA extraction and RT-qPCR from differentiated endothelial cells without the need to initially detach cells from their supporting matrix. CONCLUSIONS: We present here a new thin-layer assay (TLA) for measuring the anchorage-dependent differentiation of endothelial cells into tube-like structures which retains all the characteristics of the traditional approach but with the added benefit of a greatly lowered cost and better compatibility with other techniques, including RT-qPCR and high-resolution microscopy. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12860-014-0041-5) contains supplementary material, which is available to authorized users
Evaluation of the Role of Candida albicans Agglutinin-Like Sequence (Als) Proteins in Human Oral Epithelial Cell Interactions
The fungus C. albicans uses adhesins to interact with human epithelial surfaces in the processes of colonization and pathogenesis. The C. albicans ALS (agglutinin-like sequence) gene family encodes eight large cell-surface glycoproteins (Als1-Als7 and Als9) that have adhesive function. This study utilized C. albicans Δals mutant strains to investigate the role of the Als family in oral epithelial cell adhesion and damage, cytokine induction and activation of a MAPK-based (MKP1/c-Fos) signaling pathway that discriminates between yeast and hyphae. Of the eight Δals mutants tested, only the Δals3 strain showed significant reductions in oral epithelial cell adhesion and damage, and cytokine production. High fungal:epithelial cell multiplicities of infection were able to rescue the cell damage and cytokine production phenotypes, demonstrating the importance of fungal burden in mucosal infections. Despite its adhesion, damage and cytokine induction phenotypes, the Δals3 strain induced MKP1 phosphorylation and c-Fos production to a similar extent as control cells. Our data demonstrate that Als3 is involved directly in epithelial adhesion but indirectly in cell damage and cytokine induction, and is not the factor targeted by oral epithelial cells to discriminate between the yeast and hyphal form of C. albicans
EDITORIAL COMMENT: LAPAROSCOPIC - ASSISTED TRANSPYELIC RIGID NEPHROSCOPY - SIMPLE ALTER-NATIVE WHEN FLEXIBLE URETEROSCOPY IS NOT AVAILABLE
Genome wide association mapping of grain arsenic, copper, molybdenum and zinc in rice (Oryza sativa L.) grown at four international field sites
Peer reviewedPublisher PD
Smooth Muscle Myosin Inhibition: A Novel Therapeutic Approach for Pulmonary Hypertension
Pulmonary hypertension remains a major clinical problem despite current therapies. In this study, we examine for the first time a novel pharmacological target, smooth muscle myosin, and determine if the smooth muscle myosin inhibitor, CK-2019165 (CK-165) ameliorates pulmonary hypertension.Six domestic female pigs were surgically instrumented to measure pulmonary blood flow and systemic and pulmonary vascular dynamics. Pulmonary hypertension was induced by hypoxia, or infusion of the thromboxane analog (U-46619, 0.1 µg/kg/min, i.v.). In rats, chronic pulmonary hypertension was induced by monocrotaline.CK-165 (4 mg/kg, i.v.) reduced pulmonary vascular resistance by 22±3 and 28±6% from baseline in hypoxia and thromboxane pig models, respectively (p<0.01 and 0.01), while mean arterial pressure also fell and heart rate rose slightly. When CK-165 was delivered via inhalation in the hypoxia model, pulmonary vascular resistance fell by 17±6% (p<0.05) while mean arterial pressure and heart rate were unchanged. In the monocrotaline model of chronic pulmonary hypertension, inhaled CK-165 resulted in a similar (18.0±3.8%) reduction in right ventricular systolic pressure as compared with sildenafil (20.3±4.5%).Inhibition of smooth muscle myosin may be a novel therapeutic target for treatment of pulmonary hypertension
Modulation of enhancer looping and differential gene targeting by Epstein-Barr virus transcription factors directs cellular reprogramming
Epstein-Barr virus (EBV) epigenetically reprogrammes B-lymphocytes to drive immortalization and facilitate viral persistence. Host-cell transcription is perturbed principally through the actions of EBV EBNA 2, 3A, 3B and 3C, with cellular genes deregulated by specific combinations of these EBNAs through unknown mechanisms. Comparing human genome binding by these viral transcription factors, we discovered that 25% of binding sites were shared by EBNA 2 and the EBNA 3s and were located predominantly in enhancers. Moreover, 80% of potential EBNA 3A, 3B or 3C target genes were also targeted by EBNA 2, implicating extensive interplay between EBNA 2 and 3 proteins in cellular reprogramming. Investigating shared enhancer sites neighbouring two new targets (WEE1 and CTBP2) we discovered that EBNA 3 proteins repress transcription by modulating enhancer-promoter loop formation to establish repressive chromatin hubs or prevent assembly of active hubs. Re-ChIP analysis revealed that EBNA 2 and 3 proteins do not bind simultaneously at shared sites but compete for binding thereby modulating enhancer-promoter interactions. At an EBNA 3-only intergenic enhancer site between ADAM28 and ADAMDEC1 EBNA 3C was also able to independently direct epigenetic repression of both genes through enhancer-promoter looping. Significantly, studying shared or unique EBNA 3 binding sites at WEE1, CTBP2, ITGAL (LFA-1 alpha chain), BCL2L11 (Bim) and the ADAMs, we also discovered that different sets of EBNA 3 proteins bind regulatory elements in a gene and cell-type specific manner. Binding profiles correlated with the effects of individual EBNA 3 proteins on the expression of these genes, providing a molecular basis for the targeting of different sets of cellular genes by the EBNA 3s. Our results therefore highlight the influence of the genomic and cellular context in determining the specificity of gene deregulation by EBV and provide a paradigm for host-cell reprogramming through modulation of enhancer-promoter interactions by viral transcription factors
Polaron Effective Mass, Band Distortion, and Self-Trapping in the Holstein Molecular Crystal Model
We present polaron effective masses and selected polaron band structures of
the Holstein molecular crystal model in 1-D as computed by the Global-Local
variational method over a wide range of parameters. These results are augmented
and supported by leading orders of both weak- and strong-coupling perturbation
theory. The description of the polaron effective mass and polaron band
distortion that emerges from this work is comprehensive, spanning weak,
intermediate, and strong electron-phonon coupling, and non-adiabatic, weakly
adiabatic, and strongly adiabatic regimes. Using the effective mass as the
primary criterion, the self-trapping transition is precisely defined and
located. Using related band-shape criteria at the Brillouin zone edge, the
onset of band narrowing is also precisely defined and located. These two lines
divide the polaron parameter space into three regimes of distinct polaron
structure, essentially constituting a polaron phase diagram. Though the
self-trapping transition is thusly shown to be a broad and smooth phenomenon at
finite parameter values, consistency with notion of self-trapping as a critical
phenomenon in the adiabatic limit is demonstrated. Generalizations to higher
dimensions are considered, and resolutions of apparent conflicts with
well-known expectations of adiabatic theory are suggested.Comment: 28 pages, 15 figure
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