Early growth stages salinity stress tolerance in CM72 x Gairdner doubled haploid barley population

A doubled haploid (DH) population of barley (Hordeum vulgare L.) generated from salinity tolerant genotype CM72 and salinity sensitive variety Gairdner was studied for salinity stress tolerance at germination, seedling emergence and first leaf full expansion growth stages. Germination study was conducted with deionized water, 150 mM and 300 mM NaCl treatments. Seedling stage salinity tolerance was conducted with three treatments: control, 150 mM NaCl added at seedling emergence and first leaf full expansion growth stages. Results from this study revealed transgressive phenotypic segregations for germination percentage and biomass at seedling stage. Twelve QTL were identified on chromosomes 2H–6H each explaining 10–25% of the phenotypic variations. A QTL located at 176.5 cM on chromosome 3H was linked with fresh weight per plant and dry weight per plant in salinity stress induced at first leaf full expansion growth stage, and dry weight per plant in salinity stress induced at seedling emergence. A stable QTL for germination at both 150 and 300 mM salinity stress was mapped on chromosome 2H but distantly located from a QTL linked with seedling stage salinity stress tolerance. QTL, associated markers and genotypes identified in this study play important roles in developing salinity stress tolerant barley varieties

Multi-locus genome-wide association studies reveal novel alleles for flowering time under vernalisation and extended photoperiod in a barley MAGIC population

Optimal flowering time has a major impact on grain yield in crop species, including the globally important temperate cereal crop barley (Hordeum vulgare L.). Understanding the genetics of flowering is a key avenue to enhancing yield potential. Although bi-parental populations were used intensively to map genes controlling flowering, their lack of genetic diversity requires additional work to obtain desired gene combinations in the selected lines, especially when the two parental cultivars did not carry the genes. Multi-parent mapping populations, which use a combination of four or eight parental cultivars, have higher genetic and phenotypic diversity and can provide novel genetic combinations that cannot be achieved using bi-parental populations. This study uses a Multi-parent advanced generation intercross (MAGIC) population from four commercial barley cultivars to identify genes controlling flowering time in different environmental conditions. Genome-wide association studies (GWAS) were performed using 5,112 high-quality markers from Diversity Arrays Technology sequencing (DArT-seq), and Kompetitive allele-specific polymerase chain reaction (KASP) genetic markers were developed. Phenotypic data were collected from fifteen different field trials for three consecutive years. Planting was conducted at various sowing times, and plants were grown with/without additional vernalisation and extended photoperiod treatments. This study detected fourteen stable regions associated with flowering time across multiple environments. GWAS combined with pangenome data highlighted the role of CEN gene in flowering and enabled the prediction of different CEN alleles from parental lines. As the founder lines of the multi-parental population are elite germplasm, the favourable alleles identified in this study are directly relevant to breeding, increasing the efficiency of subsequent breeding strategies and offering better grain yield and adaptation to growing conditions

Comparative Assessment of the Binding and Neutralisation Activity of Bispecific Antibodies Against SARS-CoV-2 Variants.

Neutralising antibodies against SARS-CoV-2 are a vital component in the fight against COVID-19 pandemic, having the potential of both therapeutic and prophylactic applications. Bispecific antibodies (BsAbs) against SARS-CoV-2 are particularly promising, given their ability to bind simultaneously to two distinct sites of the receptor-binding domain (RBD) of the viral spike protein. Such antibodies are complex molecules associated with multi-faceted mechanisms of action that require appropriate bioassays to ensure product quality and manufacturing consistency. We developed procedures for biolayer interferometry (BLI) and a cell-based virus neutralisation assay, the focus reduction neutralisation test (FRNT). Using both assays, we tested a panel of five BsAbs against different spike variants (Ancestral, Delta and Omicron) to evaluate the use of these analytical methods in assessing binding and neutralisation activities of anti-SARS-CoV-2 therapeutics. We found comparable trends between BLI-derived binding affinity and FRNT-based virus neutralisation activity. Antibodies that displayed high binding affinity against a variant were often followed by potent neutralisation at lower concentrations, whereas those with low binding affinity also demonstrated reduced neutralisation activity. The results support the utility of BLI and FRNT assays in measuring variant-specific binding and virus neutralisation activity of anti-SARS-CoV-2 antibodies

Enhancement of process capabilities in electrically-assisted double sided incremental forming

© 2015 Elsevier Ltd. Electrically-assisted incremental sheet forming (E-ISF) is an effective method to improve formability by introducing the electric current in ISF process. This method is particularly useful for production of lightweight 'hard-to-form' materials such as magnesium and titanium alloys. However, the use of electricity and heat may also lead to side effects to formed components, such as unacceptable surface finish. In this work, an improved E-DSIF process has been developed by combining the electrically-assisted forming technology, the double sided incremental forming (DSIF) and a newly designed slave tool force control device to ensure stable tool-sheet contact. Different types of forming tools and toolpath strategies are explored to improve surface finish and geometrical accuracy by using a customized DSIF machine. AZ31B magnesium alloy sheets are formed into a truncated cone shape to verify the proposed E-DSIF process. In the investigation, the causes of rough surface finish are investigated in detail, and the surface finish is refined by improving the contact condition at tool-sheet interface. In addition, a hybrid toolpath strategy is proposed to further enhance the geometrical accuracy. The results demonstrate that the two challenging issues, surface finish and geometrical accuracy, could be improved by using the enhanced technologies of E-DSIF

Hund's Rule for Composite Fermions

We consider the ``fractional quantum Hall atom" in the vanishing Zeeman energy limit, and investigate the validity of Hund's maximum-spin rule for interacting electrons in various Landau levels. While it is not valid for {\em electrons} in the lowest Landau level, there are regions of filling factors where it predicts the ground state spin correctly {\em provided it is applied to composite fermions}. The composite fermion theory also reveals a ``self-similar" structure in the filling factor range $4/3>\nu>2/3$.Comment: 10 pages, revte

First long-term study of particle number size distributions and new particle formation events of regional aerosol in the North China Plain

Atmospheric particle number size distributions (size range 0.003–10 μm) were measured between March 2008 and August 2009 at Shangdianzi (SDZ), a rural research station in the North China Plain. These measurements were made in an attempt to better characterize the tropospheric background aerosol in Northern China. The mean particle number concentrations of the total particle, as well as the nucleation, Aitken, accumulation and coarse mode were determined to be 1.2 ± 0.9 × 104, 3.6 ± 7.9 × 103, 4.4 ± 3.4 × 103, 3.5 ± 2.8 × 103 and 2 ± 3 cm−3, respectively. A general finding was that the particle number concentration was higher during spring compared to the other seasons. The air mass origin had an important effect on the particle number concentration and new particle formation events. Air masses from northwest (i.e. inner Asia) favored the new particle formation events, while air masses from southeast showed the highest particle mass concentration. Significant diurnal variations in particle number were observed, which could be linked to new particle formation events, i.e. gas-to-particle conversion. During particle formation events, the number concentration of the nucleation mode rose up to maximum value of 104 cm−3. New particle formation events were observed on 36% of the effective measurement days. The formation rate ranged from 0.7 to 72.7 cm−3 s−1, with a mean value of 8.0 cm−3 s−1. The value of the nucleation mode growth rate was in the range of 0.3–14.5 nm h−1, with a mean value of 4.3 nm h−1. It was an essential observation that on many occasions the nucleation mode was able to grow into the size of cloud condensation nuclei (CCN) within a matter of several hours. Furthermore, the new particle formation was regularly followed by a measurable increase in particle mass concentration and extinction coefficient, indicative of a high abundance of condensable vapors in the atmosphere under study

Fractional Quantum Hall States in Low-Zeeman-Energy Limit

We investigate the spectrum of interacting electrons at arbitrary filling factors in the limit of vanishing Zeeman splitting. The composite fermion theory successfully explains the low-energy spectrum {\em provided the composite fermions are treated as hard-core}.Comment: 12 pages, revte

Cosmic Ray Positron and Electron Excess from Hidden-Fermion Dark Matter Decays

The anomalies observed in recent cosmic ray experiments seem to strongly constrain the nature of the dark matter. In this letter, we investigate a possibility of the fermionic dark matter with a minimal extension of the standard model. We found that the dark matter decays caused by the dimension six operators can naturally explain the anomalies.Comment: 10 pages, 8 figures, accepted by PL