13,647 research outputs found
Antioxidant tannins from Syzygium cumini fruit
Hydrolysable and condensed tannins in the fruit of Syzygium cumini were identified using NMR, MALDITOF MS and HPLC analyses. Hydrolysable tannins were identified as ellagitannins, consisting of a glucose core surrounded by gallic acid and ellagic acid units. Condensed tannins were identified as Btype oligomers of epiafzelechin (propelargonidin) with a degree of polymerization up to eleven. The antioxidant activity were measured by two vitro models: 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and ferric reducing/antioxidant power (FRAP). Tannins extracted from S. cumini fruit showed a very good DPPH radical scavenging activity and ferric reducing/antioxidant power. Theresults are promising thus indicating the utilization of the fruit of S. cumini as a significant source of natural antioxidants
Identification of sugarcane interspecies hybrids with RAPDs
Identification of “Saccharum officinarum × Erianthus fulvus” F1 hybrids was performed with random amplified polymorphic DNA (RAPD) analysis. Of 280 RAPD primers used, two primers, OPA-19 and OPN-11, were found to be the most suitable for identification of the hybrids. And the hybrids facticitycheck-out rate was 70.6 and 68.3%, respectively
Rice protein radicals: growth and stability under microwave treatment
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Development and application of a loop-mediated isothermal amplification method for rapid detection of Haemophilus parasuis
Haemophilus parasuis is the causative agent of Glässer’s disease that has received much attention recently, due to the increasing economic losses this disease inflicts upon the pig industry worldwide. In this study, loop-mediated isothermal amplification method (LAMP) methodology was designed for diagnosing H. parasuis infections and tested against 56 clinical samples. Two sets of primers for LAMP were designed based on the H. parasuis inf B gene sequence. Target DNA was amplified and visualized on agarose gels after 50 min incubation at 63°C. The LAMP amplicon was also directly visualized in the reaction tubes by the naked eye following the addition of SYBR green I. The detection limit of the inf BLAMP method was 10 cfu mL-1, that was 10 times more sensitive than conventional PCR. Furthermore, positive rates of H. parasuis detection using inf B-LAMP were higher (46.4%, 26/56) than the rates obtained with conventional PCR (33.9%, 19/56). inf B-LAMP specificity analysis demonstrated no crossreactivity with any other swine pathogens. In conclusion, inf B-LAMP was more sensitive and faster and could be carried out in the absence of expensive equipment. Furthermore, the visual readout demonstrated great potential for the use of inf B-LAMP in the clinical detection of H. parasuis.Key words: Glässer’s disease, Haemophilus parasuis, inf B, PCR, LAM
The wavelet-NARMAX representation : a hybrid model structure combining polynomial models with multiresolution wavelet decompositions
A new hybrid model structure combing polynomial models with multiresolution wavelet decompositions is introduced for nonlinear system identification. Polynomial models play an important role in approximation theory, and have been extensively used in linear and nonlinear system identification. Wavelet decompositions, in which the basis functions have the property of localization in both time and frequency, outperform many other approximation schemes and offer a flexible solution for approximating arbitrary functions. Although wavelet representations can approximate even severe nonlinearities in a given signal very well, the advantage of these representations can be lost when wavelets are used to capture linear or low-order nonlinear behaviour in a signal. In order to sufficiently utilise the global property of polynomials and the local property of wavelet representations simultaneously, in this study polynomial models and wavelet decompositions are combined together in a parallel structure to represent nonlinear input-output systems. As a special form of the NARMAX model, this hybrid model structure will be referred to as the WAvelet-NARMAX model, or simply WANARMAX. Generally, such a WANARMAX representation for an input-output system might involve a large number of basis functions and therefore a great number of model terms. Experience reveals that only a small number of these model terms are significant to the system output. A new fast orthogonal least squares algorithm, called the matching pursuit orthogonal least squares (MPOLS) algorithm, is also introduced in this study to determine which terms should be included in the final model
Modeling user mobility via user psychological and geographical behaviors towards point of-interest recommendation
© Springer International Publishing Switzerland 2016. The pervasive employments of Location-based Social Network call for precise and personalized Point-of-Interest (POI) recommendation to predict which places the users prefer. Modeling user mobility, as an important component of understanding user preference, plays an essential role in POI recommendation. However, existing methods mainly model user mobility through analyzing the check-in data and formulating a distribution without considering why a user checks in at a specific place from psychological perspective. In this paper, we propose a POI recommendation algorithm modeling user mobility by considering check-in data and geographical information. Specifically, with check-in data, we propose a novel probabilistic latent factor model to formulate user psychological behavior from the perspective of utility theory, which could help reveal the inner information underlying the comparative choice behaviors of users. Geographical behavior of all the historical check-ins captured by a power law distribution is then combined with probabilistic latent factor model to form the POI recommendation algorithm. Extensive evaluation experiments conducted on two real-world datasets confirm the superiority of our approach over state-of-the-art methods
Spin flip scattering in magnetic junctions
Processes which flip the spin of an electron tunneling in a junction made up
of magnetic electrodes are studied. It is found that: i) Magnetic impurities
give a contribution which increases the resistance and lowers the
magnetoresistance, which saturates at low temperatures. The conductance
increases at high fields. ii) Magnon assisted tunneling reduces the
magnetoresistance as , and leads to a non ohmic contribution to the
resistance which goes as , iii) Surface antiferromagnetic magnons,
which may appear if the interface has different magnetic properties from the
bulk, gives rise to and contributions to the magnetoresistance and
resistance, respectively, and, iv) Coulomb blockade effects may enhance the
magnetoresistance, when transport is dominated by cotunneling processes.Comment: 5 page
P20-08. Glycosylation: an important factor in Env diversity
Supported by a CAVD Grant from the Bill and Melinda Gates Foundation
Transferring; mapping; cloning of powdery mildew resistance gene of Haynaldia villosa and its utilization in common wheat
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