The effects of yam gruel on lowering fasted blood glucose in T2DM rats

© 2020 Xinjun Lin et al., published by De Gruyter 2020. There is increasing evidence of the linkage between type 2 diabetes mellitus (T2DM) and gut microbiota. Based on our previous studies, we investigated the hypoglycemic mechanisms of yam gruel to provide a scientific basis for its popularization and application. Wistar rats were randomly divided into control and T2DM model groups. Rats in the model group were stimulated by a high-sugar/high-fat diet combined with an intraperitoneal injection of streptozotocin to induce T2DM. The T2DM rats were further subdivided randomly into three groups: (1) DM, (2) DM + yam gruel, and (3) DM + metformin. After 4 weeks of intervention, the changes in gut microbiota, short-chain fatty acids (SCFAs) (acetic acid, propionic acid, and butyric acid), the expression of G protein-coupled receptor 43 (GPR43), glucagon-like peptide-1 (GLP-1), peptide YY (PYY), and fasted blood glucose (FBG) levels were observed. Yam gruel intervention elevated the abundance of probiotic bacteria and increased the expression of SCFAs, GPR43 receptor, GLP-1, and PYY. It also reduced FBG levels. We conclude that yam gruel can lower FBG by promoting the growth of probiotic bacteria, increasing the content of SCFAs, and enhancing the expression of GPR43 receptor to increase the content of GLP-1 and PYY in serum

Locomotion Control System Design for the LOCH Humanoid Robot

Abstract-Design of the locomotion control system for the LOCH robot is presented in this paper. Gait planning and control algorithm for uneven terrain is also considered. The LOCH robot is an adult-sized biped humanoid robot. It adopts the distributed control structure based on CAN bus, and uses a Linux operating system as the software platform. The architecture of both the hardware and software system is introduced. The emphasis is then put onto the biped planning and control. An on-line planner is designed on the basis of the inverted arm model. It generates walking gaits adaptively according to user inputs and floor flatness changes. In handling uneven floor, an imaginary foot approach is proposed to convert the problem into flat floor planning. Stability control and compliant landing control are also investigated. Both experiments and simulations are performed to show the effectiveness of the proposed design

Ethylene sensor-enabled dynamic monitoring and multi-strategies control for quality management of fruit cold chain logistics

Due to the presence of bioactive compounds, fruits are an essential part of people’s healthy diet. However, endogenous ethylene produced by climacteric fruits and exogenous ethylene in the microenvironment could play a pivotal role in the physiological and metabolic activities, leading to quality losses during storage or shelf life. Moreover, due to the variety of fruits and complex scenarios, different ethylene control strategies need to be adapted to improve the marketability of fruits and maintain their high quality. Therefore, this study proposed an ethylene dynamic monitoring based on multi-strategies control to reduce the post-harvest quality loss of fruits, which was evaluated here for blueberries, sweet cherries, and apples. The results showed that the ethylene dynamic monitoring had rapid static/dynamic response speed (2 ppm/s) and accurately monitoring of ethylene content (99% accuracy). In addition, the quality parameters evolution (firmness, soluble solids contents, weight loss rate, and chromatic aberration) showed that the ethylene multi-strategies control could effectively reduce the quality loss of fruits studied, which showed great potential in improving the quality management of fruits in the supply chain

Self-assembly of ABC-type patchy nanoparticles formed by crosslinking triblock copolymer micelles

Hierarchical self-assembly is efficient in transferring functionality from small to large scales. Polymer nanoparticles with regularity are highly desirable because they are ideal units of hierarchical self-assembly. Herein, we proposed a crosslinking strategy for generating nanoparticles from ABC linear triblock copolymers and conducted a computational study on the self-assembly of the nanoparticles. The effect of nanoparticle symmetry on aggregation behaviors was examined. We found that the symmetric patchy nanoparticles can form various low-curvature aggregates tuned by the crosslinking numbers and A/C block lengths. Increasing the asymmetry of patchy nanoparticles causes the increased curvature of assemblies, forming well-defined structures such as loops, vesicles, and wavy ribbons. Furthermore, the self-assembly kinetics reveals a step-growth nature, resembling the step polymerization for one-dimensional growth. The work demonstrates that ABC-type polymeric nanoparticles are versatile units for building hierarchical assemblies, considerably enriching the self-assembly strategies

Study on the Shear Strength of Root-Soil Composite and Root Reinforcement Mechanism

This study investigates the effects of root distributions and stress paths on the shear strength of root-soil composites using a consolidated-undrained (CU) triaxial test. On the basis of the limit equilibrium, two root reinforcement coefficients (n and m) are proposed for characterizing the effects of shear strength parameters on the principal stress considering different root distribution angles and root diameters. Then, n and m are introduced into the conventional limit equilibrium equation to develop a new limit equilibrium equation for root-soil composites. The results demonstrate that the root distribution angles (α) and root diameters (d) affect the shear strength of the root-soil composites. Under a consolidated-undrained condition, the effective cohesion (crs′) of the rooted soil is high and decreases in the order of 90°, 0°, 30° and 60°. For the same root distribution angle, crs′ increases with the increasing root diameter. Meanwhile, the effective internal friction angle (φrs′) changes slightly. The failure principal stress of the root-soil composites is positively correlated with n and m. Furthermore, the deformation of the samples indicates that the run-through rate of α = 90° and α = 0° are both 0. Meanwhile, the lateral deformation rate declines from 17.0% for α = 60° to 10.9% for α = 90°

Study on the Shear Strength of Root-Soil Composite and Root Reinforcement Mechanism

This study investigates the effects of root distributions and stress paths on the shear strength of root-soil composites using a consolidated-undrained (CU) triaxial test. On the basis of the limit equilibrium, two root reinforcement coefficients (n and m) are proposed for characterizing the effects of shear strength parameters on the principal stress considering different root distribution angles and root diameters. Then, n and m are introduced into the conventional limit equilibrium equation to develop a new limit equilibrium equation for root-soil composites. The results demonstrate that the root distribution angles (α) and root diameters (d) affect the shear strength of the root-soil composites. Under a consolidated-undrained condition, the effective cohesion (crs′) of the rooted soil is high and decreases in the order of 90°, 0°, 30° and 60°. For the same root distribution angle, crs′ increases with the increasing root diameter. Meanwhile, the effective internal friction angle (φrs′) changes slightly. The failure principal stress of the root-soil composites is positively correlated with n and m. Furthermore, the deformation of the samples indicates that the run-through rate of α = 90° and α = 0° are both 0. Meanwhile, the lateral deformation rate declines from 17.0% for α = 60° to 10.9% for α = 90°

First Experimental Evidence for Reversibility of Ammonia Loss from Asparagine

Ammonia loss from (L)-asparaginyls is a nonenzymatic reaction spontaneously occurring in all proteins and eventually resulting in damaging isoaspartate residues that hamper protein function and induce proteinopathy related to aging. Here, we discuss theoretical considerations supporting the possibility of a full repair reaction and present the first experimental evidence of its existence. If confirmed, the true repair of (L)-asparaginyl deamidation could open new avenues for preventing aging and neurodegenerative diseases

Radiotherapy Side Effects: Comprehensive Proteomic Study Unraveled Neural Stem Cell Degenerative Differentiation upon Ionizing Radiation

Cranial radiation therapy is one of the most effective treatments for childhood brain cancers. Despite the ameliorated survival rate of juvenile patients, radiation exposure-induced brain neurogenic region injury could markedly impair patients’ cognitive functions and even their quality of life. Determining the mechanism underlying neural stem cells (NSCs) response to irradiation stress is a crucial therapeutic strategy for cognitive impairment. The present study demonstrated that X-ray irradiation arrested NSCs’ cell cycle and impacted cell differentiation. To further characterize irradiation-induced molecular alterations in NSCs, two-dimensional high-resolution mass spectrometry-based quantitative proteomics analyses were conducted to explore the mechanism underlying ionizing radiation’s influence on stem cell differentiation. We observed that ionizing radiation suppressed intracellular protein transport, neuron projection development, etc., particularly in differentiated cells. Redox proteomics was performed for the quantification of cysteine thiol modifications in order to profile the oxidation-reduction status of proteins in stem cells that underwent ionizing radiation treatment. Via conjoint screening of protein expression abundance and redox status datasets, several significantly expressed and oxidized proteins were identified in differentiating NSCs subjected to X-ray irradiation. Among these proteins, succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (sdha) and the acyl carrier protein, mitochondrial (Ndufab1) were highly related to neurodegenerative diseases such as Parkinson’s disease, Alzheimer’s disease, and Huntington’s disease, illustrating the dual-character of NSCs in cell differentiation: following exposure to ionizing radiation, the normal differentiation of NSCs was compromised, and the upregulated oxidized proteins implied a degenerative differentiation trajectory. These findings could be integrated into research on neurodegenerative diseases and future preventive strategies

Response of transpiration to rain pulses for two tree species in a semiarid plantation

Responses of transpiration (E (c)) to rain pulses are presented for two semiarid tree species in a stand of Pinus tabulaeformis and Robinia pseudoacacia. Our objectives are to investigate (1) the environmental control over the stand transpiration after rainfall by analyzing the effect of vapor pressure deficit (VPD), soil water condition, and rainfall on the post-rainfall E (c) development and recovery rate, and (2) the species responses to rain pulses and implications on vegetation coverage under a changing rainfall regime. Results showed that the sensitivity of canopy conductance (G (c)) to VPD varied under different incident radiation and soil water conditions, and the two species exhibited the same hydraulic control (-dG (c)/dlnVPD to G (cref) ratio) over transpiration. Strengthened physiological control and low sapwood area of the stand contributed to low E (c). VPD after rainfall significantly influenced the magnitude and time series of post-rainfall stand E (c). The fluctuation of post-rainfall VPD in comparison with the pre-rainfall influenced the E (c) recovery. Further, the stand E (c) was significantly related to monthly rainfall, but the recovery was independent of the rainfall event size. E (c) enhanced with cumulative soil moisture change (∆VWC) within each dry-wet cycle, yet still was limited in large rainfall months. The two species had different response patterns of post-rainfall E (c) recovery. E (c) recovery of P. tabulaeformis was influenced by the pre- and post-rainfall VPD differences and the duration of rainless interval. R. pseudoacacia showed a larger immediate post-rainfall E (c) increase than P. tabulaeformis did. We, therefore, concluded that concentrated rainfall events do not trigger significant increase of transpiration unless large events penetrate the deep soil and the species differences of E (c) in response to pulses of rain may shape the composition of semiarid woodlands under future rainfall regimes.13 page(s

Ultralight Ultrafast Enzymes

Inorganic materials depleted of heavy stable isotopes are known to deviate strongly in some physicochemical properties from their isotopically natural counterparts. Here we explored for the first time the effect of simultaneous depletion of the heavy carbon, hydrogen, oxygen and nitrogen isotopes on the bacterium E. coli and the enzymes expressed in it. Bacteria showed faster growth, with most proteins exhibiting higher thermal stability, while for recombinant enzymes expressed in depleted media, faster kinetics was discovered. At room temperature, luciferase, thioredoxin and dihydrofolate reductase and Pfu DNA polymerase showed up to a 250 % increase in activity compared to the native counterparts, with an additional ∼50 % increase at 10 °C. Diminished conformational and vibrational entropy is hypothesized to be the cause of the accelerated kinetics. Ultralight enzymes may find an application where extreme reaction rates are required.</p