Dynamic allosteric control of noncovalent DNA catalysis reactions

Allosteric modulation of catalysis kinetics is prevalent in proteins and has been rationally designed for ribozymes. Here, we present an allosteric DNA molecule that, in its active configuration, catalyzes a noncovalent DNA reaction. The catalytic activity is designed to be modulated by the relative concentrations of two DNA regulator molecules, one an inhibitor and the other an activator. Dynamic control of the catalysis rate is experimentally demonstrated via three cycles of up and down regulation by a factor of over 10. Unlike previous works, both the allosteric receptor and catalytic core are designed, rather than evolved. This allows flexibility in the sequence design and modularity in synthetic network construction

Control of DNA Strand Displacement Kinetics Using Toehold Exchange

DNA is increasingly being used as the engineering material of choice for the construction of nanoscale circuits, structures, and motors. Many of these enzyme-free constructions function by DNA strand displacement reactions. The kinetics of strand displacement can be modulated by toeholds, short single-stranded segments of DNA that colocalize reactant DNA molecules. Recently, the toehold exchange process was introduced as a method for designing fast and reversible strand displacement reactions. Here, we characterize the kinetics of DNA toehold exchange and model it as a three-step process. This model is simple and quantitatively predicts the kinetics of 85 different strand displacement reactions from the DNA sequences. Furthermore, we use toehold exchange to construct a simple catalytic reaction. This work improves the understanding of the kinetics of nucleic acid reactions and will be useful in the rational design of dynamic DNA and RNA circuits and nanodevices

Infinite type germs of smooth hypersurfaces in Cn\mathbb C^n

In this paper, we discuss germs of smooth hypersurface in $\mathbb C^n$. We show that if a point on the boundary has infinite D'Angelo type, then there exists a formal complex curve in the hypersurface through that point.Comment: 6 pages. published in complex. Var. Elliptic. Equ.200

Proceedings of the Symposium on Concrete Modelling, CONMOD2018

CONMOD2018 is a symposium on Concrete Modelling which is jointly organised by Delft University and Ghent University as part of the RILEM week 2018 in Delft, The Netherlands. The symposium is the 5th in a series dealing with all aspects concerning modelling of concrete at various scales. The symposium consist of 3 key-note papers and 62 regular papers presented over 3 days. Parallel to the CONMOD2018 symposium a conference on Service Life Design (SLD4) and a workshop honouring Professor Klaas van Breugel were organised with topics that are related to concrete modelling. In total more than 350 participants took part in the events organised during the RILEM week 2018