Detection of MTAP Protein and Gene Expression in Non-small Cell Lung Cancer

Background and objective The abnormal expression of MTAP, a tumor suppressor gene, is found in a variety of tumor tissues. The aim of this study is to detect the expression of MTAP mRNA protein and the clinical significance for the therapy of non-small cell lung cancer tissue (NSCLC). Methods The expression of MTAP protein was detected by immunohistochemistry in 52 cases of NSCLC patients. The relative expression MTAP mRNA was detected by real-time quantitative PCR. Results The expression of MTAP protein in NSCLC tissue was significantly lower than that in paracarcinomous tissue and borderline lung tissue respectively (t=10.283, 10.940, P < 0.001). There was no significant difference among gender, age, smoking history, histology except differentiation (t=2.310, P=0.025). The MTAP mRNA relative expression in NSCLC tissue was significantly lower than that in paracarcinomous tissue (t=9.902, P < 0.001). Conclusion Downregulation of MTAP protein and gene expression is correlated to the oncogenesis and progression of NSCLC

A meta-analysis of the effect of Sjögren′s syndrome on adverse pregnancy outcomes

Objective: This study aimed to assess the correlation between Sjögren′s Syndrome (SS) and adverse pregnancy outcomes, with the aim of providing a basis for preconception and pregnancy interventions in women with SS. Methods: A search of electronic databases in English and Chinese databases from January 2005 to December 2021, was conducted to collect the literature of case-control studies or cohort studies on the association between SS and pregnancy outcome studies. Literature inclusion and data extraction were performed according to established criteria, and the Newcastle-Ottawa scale was used to evaluate the quality of the literature. Stata 15 software was used for meta-analysis. Results: A total of nine papers were included in this study. Meta-analysis results showed that SS was associated with spontaneous abortion (RR = 8.85, 95% CI 3.10‒25.26), preterm birth (RR = 2.27, 95% CI 1.46‒3.52), low birth mass (RR = 1.99, 95% CI 1.34‒2.97), and birth defects (RR = 4.28, 95% CI 3.08‒5.96). Conclusion: SS can increase the incidence of adverse pregnancy outcomes

Design and application of fuel injection drive circuit for engine test bench

Based on the STM32 library function, this design is developed a good docking with the engine, without changing the engine's original ECU. Only on the test bench, this design which connected to the device can enter the injection pulse width, so as to control the engine mixture concentration. Using this experimental device, the fuel adjustment characteristic experiment was completed on the existing engine test bed, which verified that the experimental device developed by this design had good adaptability in use. In this way, universities and research institutes can use this experimental device to easily complete the fuel adjustment characteristic experiment, and can also provide a convenient tool option for the calibration and optimization of the engine pulse spectrum

An Oil Spill Spatial Data Model for Qinzhou Bay Based on the KML

Qinzhou Bay is an important channel of Chinese southwest goes to sea. The Qinzhou Bay was chosen as the study area in this paper. Using the international advanced model Oilmap, Analysis of oil spill on the initial oil membrane formation factors, exclude some of initial oil film effects are not important factors, Find suitable for oil spill earlier an oil spill force model. Combined with the natural condition of Qinzhou Bay, The transport process of oil membrane in the condition of different wind and current was observed in Qinzhou Bay by means of experiments. This paper analyzes the main factors, such as the shape of oil membrane and migration directions, which leads to the oil spill, and got a suitable model of oil spill to Qinzhou Bay. In order to achieve its visual in the software of geographic information system, the model of oil spill was defined through the KML

Investigations into the effects of scaffold microstructure on slow-release system with bioactive factors for bone repair

In recent years, bone tissue engineering (BTE) has played an essential role in the repair of bone tissue defects. Although bioactive factors as one component of BTE have great potential to effectively promote cell differentiation and bone regeneration, they are usually not used alone due to their short effective half-lives, high concentrations, etc. The release rate of bioactive factors could be controlled by loading them into scaffolds, and the scaffold microstructure has been shown to significantly influence release rates of bioactive factors. Therefore, this review attempted to investigate how the scaffold microstructure affected the release rate of bioactive factors, in which the variables included pore size, pore shape and porosity. The loading nature and the releasing mechanism of bioactive factors were also summarized. The main conclusions were achieved as follows: i) The pore shapes in the scaffold may have had no apparent effect on the release of bioactive factors but significantly affected mechanical properties of the scaffolds; ii) The pore size of about 400 μm in the scaffold may be more conducive to controlling the release of bioactive factors to promote bone formation; iii) The porosity of scaffolds may be positively correlated with the release rate, and the porosity of 70%–80% may be better to control the release rate. This review indicates that a slow-release system with proper scaffold microstructure control could be a tremendous inspiration for developing new treatment strategies for bone disease. It is anticipated to eventually be developed into clinical applications to tackle treatment-related issues effectively

Simultaneous quantification of 6,7-di-hydroxyligustilide and gastrodin in rat plasma by LC-MS: application to pharmacokinetic study of tianshu capsule

A LC-MS method was developed and validated for simultaneous determination of 6, 7-di-hydroxyligustilide and gastrodin in rat plasma, and which was subsequently applied in the pharmacokinetic analysis of an administration of a Chinese herbal extract containing Chuanxiong Rhizoma and Gastrodia Elata Rhizome, i.e. TianShu capsule against migraine. The analytes were separated on a Kromasil C18 column with a gradient elution program and detected without interference in the selected ion monitoring mode with positive electrospray ionization. The linear range was 0.010-10.0 μg/mL for 6,7-di-hydroxyligustilide and 0.025-25.0 μg/mL for gastrodin with the limit of quantitation of 0.01 and 0.025 μg/mL, respectively. The intra-day and inter-day precisions for the entire validation were less than14.7 % of RSD. The pharmacokinetic parameters indicated that 6, 7-di-hydroxyligustilide and gastrodin are absorbed rapidly and reached a maximum concentration within one hour, which was consistent with the clinical requirements for the rapid relieving the symptoms of migraine.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Global research trends of Mycoplasma pneumoniae pneumonia in children: a bibliometric analysis

BackgroundMycoplasma pneumoniae pneumonia (MPP), attributable to Mycoplasma pneumoniae (MP), represents a predominant form of community-acquired pneumonia in pediatric populations, thereby posing a significant threat to pediatric health. Given the burgeoning volume of research literature associated with pediatric MPP in recent years, it becomes imperative to undertake a bibliometric analysis aimed at delineating the current research landscape and emerging trends, thereby furnishing a framework for subsequent investigations.MethodsA comprehensive literature search targeting pediatric MPP was conducted in the Web of Science Core Collection. After the removal of duplicate entries through Endnote software, the remaining articles were subject to scientometric analysis via Citespace software, VOSviewer software and R language, focusing on variables such as publication volume, contributing nations, institutions and authors, references and keywords.ResultsA total of 1,729 articles pertinent to pediatric MPP were included in the analysis. China and the United States emerged as the nations with the highest publication output. Italian scholar Susanna Esposito and Japanese scholar Kazunobu Ouchi were the most influential authors in the domain of pediatric MPP. Highly-cited articles primarily focused on the epidemiological investigation of pediatric MPP, the clinical characteristics and treatment of macrolide-resistant MPP, and biomarkers for refractory Mycoplasma pneumoniae pneumonia (RMPP). From the corpus of 1,729 articles, 636 keywords were extracted and categorized into ten clusters: Cluster #0 centered on molecular-level typing of macrolide-resistant strains; Cluster #1 focused on lower respiratory tract co-infections; Clusters #2 and #6 emphasized other respiratory ailments caused by MP; Cluster #3 involved biomarkers and treatment of RMPP; Clusters #4 and #9 pertained to extrapulmonary complications of MPP, Clusters #5 and #7 addressed etiological diagnosis of MPP, and Cluster #8 explored pathogenic mechanisms.ConclusionsThe past few years have witnessed extensive attention directed towards pediatric MPP. Research in pediatric MPP principally revolves around diagnostic techniques for MP, macrolide resistance, complications of MPP, treatment and diagnosis of RMPP, and elucidation of pathogenic mechanisms. The present study provides pediatric clinicians and researchers with the research status and focal points in this field, thereby guiding the orientation of future research endeavors

MoWLD: A Robust Motion Image Descriptor for Violence Detection

Abstract Automatic violence detection from video is a hot topic for many video surveillance applications. However, there has been little success in designing an algorithm that can detect violence in surveillance videos with high performance. Existing methods typically apply the Bagof-Words (BoW) model on local spatiotemporal descriptors. However, traditional spatiotemporal features are not discriminative enough, and also the BoW model roughly assigns each feature vector to only one visual word and therefore ignores the spatial relationships among the features. To tackle these problems, in this paper we propose a novel Motion Weber Local Descriptor (MoWLD) in the spirit of the well-known WLD and make it a powerful and robust descriptor for motion images. We extend the WLD spatial descriptions by adding a temporal component to the appearance descriptor, which implicitly captures local motion information as well as low-level image appear information. To eliminate redundant and irrelevant features, the nonparametric Kernel Density Estimation (KDE) is employed on the MoWLD descriptor. In order to obtain more discriminative features, we adopt the sparse coding and max pooling scheme to further process the selected MoWLDs. Experimental results on three benchmark datasets have demonstrated the superiority of the proposed approach over the state-of-the-arts