Reconstruction of shale image based on Wasserstein Generative Adversarial Networks with gradient penalty

 Generative Adversarial Networks (GANs), as most popular artificial intelligence models in the current image generation field, have excellent image generation capabilities. Based on Wasserstein GANs with gradient penalty, this paper proposes a novel digital core reconstruction method. First, a convolutional neural network is used as a generative network to learn the distribution of real shale samples, and then a convolutional neural network is constructed as a discriminative network to distinguish reconstructed shale samples from real ones. Through this confrontation training method, realistic digital core samples of shale can be reconstructed. The paper uses two-point covariance function, Frechet Inception Distance and Kernel Inception Distance, to evaluate the quality of digital core samples of shale reconstructed by GANs. The results show that the covariance function can test the similarity between generated and real shale samples, and that GANs can efficiently reconstruct digital core samples of shale with high-quality. Compared with multiple point statistics, the new method does not require prior inference of the probability distribution of the training data, and directly uses noise vector to generate digital core samples of shale without using constraints of "hard data" in advance. It is easy to produce an unlimited number of new samples. Furthermore, the training time is also shorter, only 4 hours in this paper. Therefore, the new method has some good points compared with current methods.Cited as: Zha, W., Li, X., Xing, Y., He, L., Li, D. Reconstruction of shale image based on Wasserstein Generative Adversarial Networks with gradient penalty. Advances in Geo-Energy Research, 2020, 4(1): 107-114, doi: 10.26804/ager.2020.01.1

KDM2B/FBXL10 targets c-Fos for ubiquitylation and degradation in response to mitogenic stimulation.

KDM2B (also known as FBXL10) controls stem cell self-renewal, somatic cell reprogramming and senescence, and tumorigenesis. KDM2B contains multiple functional domains, including a JmjC domain that catalyzes H3K36 demethylation and a CxxC zinc-finger that recognizes CpG islands and recruits the polycomb repressive complex 1. Here, we report that KDM2B, via its F-box domain, functions as a subunit of the CUL1-RING ubiquitin ligase (CRL1/SCF(KDM2B)) complex. KDM2B targets c-Fos for polyubiquitylation and regulates c-Fos protein levels. Unlike the phosphorylation of other SCF (SKP1-CUL1-F-box)/CRL1 substrates that promotes substrates binding to F-box, epidermal growth factor (EGF)-induced c-Fos S374 phosphorylation dissociates c-Fos from KDM2B and stabilizes c-Fos protein. Non-phosphorylatable and phosphomimetic mutations at S374 result in c-Fos protein which cannot be induced by EGF or accumulates constitutively and lead to decreased or increased cell proliferation, respectively. Multiple tumor-derived KDM2B mutations impaired the function of KDM2B to target c-Fos degradation and to suppress cell proliferation. These results reveal a novel function of KDM2B in the negative regulation of cell proliferation by assembling an E3 ligase to targeting c-Fos protein degradation that is antagonized by mitogenic stimulations

Dietary Probiotic Bacillus licheniformis TC22 Increases Growth, Immunity, and Disease Resistance, against Vibrio splendidus Infection in Juvenile Sea Cucumbers Apostichopus japonicus

In this study we examined the effects of probiotic Bacillus licheniformis TC22 on growth, immunity, and disease resistance against Vibrio splendidus in juvenile sea cucumbers Apostichopus japonicus. For 30 days, sea cucumbers were fed diets with TC22 at 0 (control), 105, 107, and 109 CFU/g respectively. Results showed that dietary TC22 at 109 CFU/g significantly improved (P0.05). Dietary TC22 at 109 CFU/g significantly improved phagocytosis, and total nitric oxide synthase activity in sea cucumbers (P0.05). Respiratory burst in sea cucumbers fed dietary TC22 at 109 CFU/g was significantly higher than those fed dietary TC22 at 107 CFU/g (P<0.05). Cumulative mortality after V. splendidus challenge decreased significantly in the sea cucumbers fed with TC22 at 109 CFU/g (P<0.05). The present study confirmed dietary B. licheniformis TC22 at 109 CFU/g could significantly improve immunity and disease resistance in juvenile A. japonicus

Dynamic characteristics of printing cylinder with rotating dynamic load

The structural vibration of the center impression cylinder in the flexographic printing machine will bring about production quality problems such as printing ghosts. To this problem, it become an important method to analysis the dynamic characteristics of the center impression cylinder. In this paper, a three-dimensional structural model of the central impression cylinder is established, and builds the dynamic model in finite element software. The vibration modes of the central impression cylinder were studied in the simple-supported system at both ends of the static impression cylinder. In view of the real existence of the rotating dynamic load, the evolution law of the natural frequency and mode shape of the cylinder with the rotating dynamic load is discussed. It is found that as the rotation speed increases, the natural frequency undergoes two steps, and the natural frequency after each step will keep continuity with the high first-order natural frequency, and the modal shape is the same as the high-order original mode. Then, the mode shape is continuously changed, not have a step

miR-200a attenuated oxidative stress, inflammation, and apoptosis in dextran sulfate sodium-induced colitis through activation of Nrf2

IntroductionOxidative stress and inflammatory responses are critical factors in ulcerative colitis disease pathogenesis. Nuclear factor erythroid 2-related factor 2 (Nrf2) modulates oxidative stress and suppresses inflammatory responses, and the protective benefits of Nrf2 activation have been associated with the therapy of ulcerative colitis. MicroRNA-200a (miR-200a) could target Kelch-like ECH-associated protein 1 (Keap1) and activate the Nrf2-regulated antioxidant pathway. Nevertheless, whether miR-200a modulates the Keap1/Nrf2 pathway in dextran sulfate sodium (DSS)-induced colonic damage is unknown. Here, our research intends to examine the impact of miR-200a in the model of DSS-induced colitis.MethodsPrior to DSS intervention, we overexpressed miR-200a in mice for four weeks using an adeno-associated viral (AAV) vector to address this problem. ELISA detected the concentration of inflammation-related cytokines. The genes involved in inflammatory reactions and oxidative stress were identified using quantitative reverse transcription-polymerase chain reaction (qRT-PCR), western blot, and immunofluorescence. Moreover, we applied siRNAs to weakened Nrf2 expression to confirm the hypothesis that miR-200a provided protection via Nrf2.ResultsThe present study discovered miR-200a down-regulation, excessive inflammatory activation, enterocyte apoptosis, colonic dysfunction, and Keap1/Nrf2 antioxidant pathway inactivation in mouse colitis and NCM460 cells under DSS induction. However, our data demonstrated that miR-200a overexpression represses Keap1 and activates the Nrf2 antioxidant pathway, thereby alleviating these adverse alterations in animal and cellular models. Significantly, following Nrf2 deficiency, we failed to observe the protective benefits of miR-200a against colonic damage.DiscussionTaken together, through activating the Keap1/Nrf2 signaling pathway, miR-200a protected against DSS-induced colonic damage. These studies offer an innovative therapeutic approach for ulcerative colitis

Dynamic characteristics of printing cylinder with rotating dynamic load

The structural vibration of the center impression cylinder in the flexographic printing machine will bring about production quality problems such as printing ghosts. To this problem, it become an important method to analysis the dynamic characteristics of the center impression cylinder. In this paper, a three-dimensional structural model of the central impression cylinder is established, and builds the dynamic model in finite element software. The vibration modes of the central impression cylinder were studied in the simple-supported system at both ends of the static impression cylinder. In view of the real existence of the rotating dynamic load, the evolution law of the natural frequency and mode shape of the cylinder with the rotating dynamic load is discussed. It is found that as the rotation speed increases, the natural frequency undergoes two steps, and the natural frequency after each step will keep continuity with the high first-order natural frequency, and the modal shape is the same as the high-order original mode. Then, the mode shape is continuously changed, not have a step