LONG-TERM CHANGES IN CANADA GOOSE NEST SUCCESS AND NEST DENSITIES AT AN IOWA WETLAND COMPLEX

Giant Canada geese (Branta canadensis maxima) were extirpated from Iowa by the early 1900s due to unregulated hunting, egg gathering, and wetland drainage in the nineteenth century (Bishop 1978). Ef- forts to reintroduce Canada geese in Iowa began in 1964 (Bishop and Howing 1972) and involved releasing flightless adults and goslings at nearly 30 sites across the state (Zenner and LaGrange 1998a). In 1972, 13 flightless pairs were released at Rice Lake Wildlife Management Area (WMA; Bishop 1978). By 1989, the breeding population of Canada geese at Rice Lake WMA had increased to 420 nesting adults (G. G. Zenner, Iowa Department of Natural Resources, unpublished data). Canada goose nest success and nest densities were documented from 1989–1991 on extant islands at Rice Lake WMA (Zenner and LaGrange 1998b). Rice Lake WMA (43.379497, –93.472715) is located in north-central Iowa and lies within the southernmost portion of the Prairie Pothole Region. This wetland complex consists of Rice Lake, a 409-ha shallow, natural lake with a maximum depth of 3 m and 20 natural islands ranging in size from 0.04 to 3.9 ha, and Joice Slough, a 73-ha marsh with a maximum depth of 1 m and 15 natural islands ranging in size from 0.02 to 3.19 ha (Zenner and LaGrange 1998b). During 1989–1991, potential Canada goose nest sites included islands, elevated structures, and muskrat houses. Over the course of that study, drought conditions left Joice Slough completely dry and dramatically lowered water levels at Rice Lake, exposing islands to increased predator activity. Despite the drought, nest densities were high (68–158 nests/ha) and nest success ranged from 40–58% (Zenner and LaGrange 1998b)

Systems model of T cell receptor proximal signaling reveals emergent ultrasensitivity

Receptor phosphorylation is thought to be tightly regulated because phosphorylated receptors initiate signaling cascades leading to cellular activation. The T cell antigen receptor (TCR) on the surface of T cells is phosphorylated by the kinase Lck and dephosphorylated by the phosphatase CD45 on multiple immunoreceptor tyrosine-based activation motifs (ITAMs). Intriguingly, Lck sequentially phosphorylates ITAMs and ZAP-70, a cytosolic kinase, binds to phosphorylated ITAMs with differential affinities. The purpose of multiple ITAMs, their sequential phosphorylation, and the differential ZAP-70 affinities are unknown. Here, we use a systems model to show that this signaling architecture produces emergent ultrasensitivity resulting in switch-like responses at the scale of individual TCRs. Importantly, this switch-like response is an emergent property, so that removal of multiple ITAMs, sequential phosphorylation, or differential affinities abolishes the switch. We propose that highly regulated TCR phosphorylation is achieved by an emergent switch-like response and use the systems model to design novel chimeric antigen receptors for therapy

LONG-TERM CHANGES IN CANADA GOOSE NEST SUCCESS AND NEST DENSITIES AT AN IOWA WETLAND COMPLEX

Giant Canada geese (Branta canadensis maxima) were extirpated from Iowa by the early 1900s due to unregulated hunting, egg gathering, and wetland drainage in the nineteenth century (Bishop 1978). Ef- forts to reintroduce Canada geese in Iowa began in 1964 (Bishop and Howing 1972) and involved releasing flightless adults and goslings at nearly 30 sites across the state (Zenner and LaGrange 1998a). In 1972, 13 flightless pairs were released at Rice Lake Wildlife Management Area (WMA; Bishop 1978). By 1989, the breeding population of Canada geese at Rice Lake WMA had increased to 420 nesting adults (G. G. Zenner, Iowa Department of Natural Resources, unpublished data). Canada goose nest success and nest densities were documented from 1989–1991 on extant islands at Rice Lake WMA (Zenner and LaGrange 1998b). Rice Lake WMA (43.379497, –93.472715) is located in north-central Iowa and lies within the southernmost portion of the Prairie Pothole Region. This wetland complex consists of Rice Lake, a 409-ha shallow, natural lake with a maximum depth of 3 m and 20 natural islands ranging in size from 0.04 to 3.9 ha, and Joice Slough, a 73-ha marsh with a maximum depth of 1 m and 15 natural islands ranging in size from 0.02 to 3.19 ha (Zenner and LaGrange 1998b). During 1989–1991, potential Canada goose nest sites included islands, elevated structures, and muskrat houses. Over the course of that study, drought conditions left Joice Slough completely dry and dramatically lowered water levels at Rice Lake, exposing islands to increased predator activity. Despite the drought, nest densities were high (68–158 nests/ha) and nest success ranged from 40–58% (Zenner and LaGrange 1998b)

Protective Immunity against Congenital Toxoplasmosis with Recombinant SAG1 Protein in a Guinea Pig Model

Primary infection with Toxoplasma gondii during pregnancy can induce fetal pathology and abortion in both humans and animals. The present study describes the development of an experimental model of congenital toxoplasmosis in the guinea pig. In this animal model, we evaluated the protective effect of vaccination with a recombinant form of SAG1 against maternofetal transmission of tachyzoites. The presence of parasites in fetuses was determined by nested PCRs and by an in vivo readout after fetal brain homogenate injections in mice. The absence of parasites was demonstrated in 66 to 86% of fetuses derived from adult guinea pigs immunized with SAG1 and challenged with the mildly virulent T. gondii strain C56. In contrast, more than 80% of fetuses from mock-immunized guinea pigs were infected. The protection was not correlated with titers of antibody to SAG1. Our results indicated that this experimental model constitutes a relevant model for evaluation of vaccine candidates against congenital toxoplasmosis and that SAG1 elicits significant protection against maternofetal transmission