Equine endometrosis pathological features: are they dependent on NF-κB signaling pathway?

Research Areas: Agriculture ; Veterinary Sciences ; ZoologyEndometrosis is an important mares’ disease which considerably decreases their fertility. As classic endometrial classification methods might be insufficient for tissue pathological evaluation, further categorization into active/inactive and destructive/non-destructive types was developed by Hoffmann and others. This study aimed to compare NF-κB pathway genes transcription among histopathological types of endometrosis, following Hoffmann and co-authors’ classification. Endometrial samples, collected postmortem from cyclic mares (n = 100) in estrus or diestrus, were classified histologically and used for gene transcription assessment. Gene transcription of NF-κB subunits (RelA, NF-κB1, NF-κB2), pro-inflammatory molecules (MCP-1, IL-6), and hyaluronan synthases (HAS 1, HAS 2, HAS 3) was compared among endometrosis types (active, non-active, destructive, non-destructive). Most individual mRNA samples showed high expression of RelA, NF-κB1, and MCP-1 gene transcripts and the destructive type of endometrosis, simultaneously. The expression of RelA and NF-κB1 genes was higher in active destructive group than in the other groups only in the follicular phase, as well as being higher in the inactive destructive group than in the others, only in the mid-luteal phase. The increase in gene transcription of the NF-κB canonical activation pathway in destructive endometrosis may suggest the highest changes in extracellular matrix deposition. Moreover, the estrous cycle phase might influence fibrosis pathogenesis.info:eu-repo/semantics/publishedVersio

Criteria for the selection of passenger air transport as part of Poland’s logistics support system

Polskie porty lotnicze pełnią kluczową funkcję w systemie wsparcia logistycznego kraju. Chcąc obsłużyć możliwie największą liczbę pasażerów powinny one poszukiwać innowacyjnych rozwiązań. Dlatego też, istotne jest by porty lotnicze dokonywały badań kryteriów wyboru pasażerskiego transportu lotniczego w celach realizacji procesów podstawowych realizowanych przez społeczeństwo. Opierając się na nich, istnieje możliwość poznania zachowań transportowych pasażerów, co pozwoliłoby na wprowadzenie zmian w strukturach organizacyjnych portów lotniczych, które skuteczniej zaspokajały ich potrzeby transportowe.Polish airports play a key role in the country's logistic support system. In order to serve as many passengers as possible, they should look for innovative solutions. Therefore, it is essential that airports research the criteria for selecting passenger air transport in order to implement basic processes carried out by society. Based on them, it is possible to learn about the transport behavior of passengers, which would allow for changes in the organizational structures of airports to meet their transport needs more effectively

A phonological description of Wichí: The dialect of Misión La Paz, Salta, Argentina

The Wichí language, a member of the Matacoan language family, is spoken in parts of South America’s Chaco region. Specifically, Wichí is found in the Salta, Chaco, and Formosa provinces of Argentina, as well as in southeastern Bolivia. Wichí is an endangered language, with speakers numbering c. 25,000 (although estimates vary greatly). This study aims to contribute to the documentation of Wichí and to our understanding of human language in general by providing insight into the phonetics and phonology of the dialect of Wichí spoken in the remote village of Misión La Paz, Salta, Argentina. The data for this study were collected in Misión La Paz during two 4-week periods in the summers of 2005 and 2006

The application of High-Speed camera (HS), acoustic analysis and Voice Handicap Index (VHI) questionnaire in diagnosis of voice disorders in elderly men

Objective: The process of ageing begins after 60 years of age and is referred to as presbyphonia (Vox senium). The causes include functional or organic voice disorders, often coexisting with dry upper respiratory tract infection. Introduction: The aim of the study is the use of high-speed camera and acoustic voice analysis in diagnostics of the clinical form of presbyphonia. M aterials and methods: The study included a group of 50 men, non-smokers, age from 51 to 72, who do not use their voice professionally. High-Speed Digital Imaging and HS camera have been used, allowing evaluation of real vibrations of vocal folds, along with acoustic voice analysis using a software by DiagNova Technologies. Results: VHI questionnaire has been used for self-assessment of voice disability. Visualizations of the larynx enabled recognition of hypofunctional dysphonia or atrophy of vocal folds that cause voice disorders. This was confirmed by parameters of voice acoustic evaluation: F0, NHR, narrowband spectrography. The pathological value of NHR and the presence of nonharmonic components in the range of high frequency levels indicated glottal insufficiency, recorded with the visualization technique of the larynx by HS camera. A significant shortening of maximum phonation time in relation to the control group has also been recorded. Discussion: The objective examination of voice pathology is crucial in diagnosis and rehabilitation, however, subjective assessment of the patient is important in the scope of the procedure used. The patient’s subjective self-rating assessment (VHI) confirmed the sense of voice disorders in elderly men, indicating the need for rapid and accurate clinical diagnosis

Zastosowanie kamery do zdjęć szybkich – High Speed (HS), badania akustycznego oraz kwestionariusza VHI w diagnostyce zaburzeń jakości głosu u mężczyzn w wieku podeszłym

Wstęp: Starzenie się głosu rozpoczyna się po 60. roku życia i jest określane mianem presbyphonii lub vox senium. Przyczyną tego procesu są zmiany o charakterze czynnościowym i organicznym w obrębie krtani, ze współistniejącym suchym nieżytem górnych dróg oddechowych. C el: Celem pracy jest wykorzystanie kamery do zdjęć szybkich – High-Speed (HS) oraz badania akustycznego głosu w diagnostyce postaci klinicznej presbyphonii u mężczyzn w wieku podeszłym. Materiały i metody: Badaniem objęto grupę 50 mężczyzn w wieku od 51 do 72 lat, bez obciążenia zawodowego głosu. W diagnostyce zastosowano technikę wizualizacji krtani, pozwalającą na ocenę drgań rzeczywistych fałdów głosowych (HSDI), oraz badanie akustyczne głosu z wykorzystaniem oprogramowania komputerowego f. DiagNova Technologies. W samoocenie głosu pacjentów zastosowano kwestionariusz Voice Handicap Index (VHI). Wyniki: Wizualizacja krtani pozwoliła na rozpoznanie dysfonii hipofunkcjonalnej lub atrofii fałdów głosowych, będących przyczyną zaburzeń głosu w diagnozowanej grupie. Potwierdziły to parametry oceny akustycznej głosu, szczególnie: F0, NHR oraz spektrografia wąskopasmowa. Patologiczna wartość parametru NHR oraz obecność składowych nieharmonicznych w zakresie wysokich poziomów częstotliwości w spektrografii wąskopasmowej były potwierdzeniem niedowładu fonacyjnego głośni, rejestrowanego techniką wizualizacyjną krtani z użyciem kamery HS. Rejestrowano również istotne skrócenie maksymalnego czasu fonacji (MPT) w stosunku do grupy kontrolnej. Dyskusja: Badanie obiektywne patologii głosu jest kluczowe w diagnostyce i monitorowaniu terapii i rehabilitacji, jednak subiektywna ocena pacjenta, szczególnie efektów jego leczenia, ma istotne znaczenie w zakresie stosowanej procedury. Subiektywna samoocena głosu pacjenta z zastosowaniem kwestionariusza VHI potwierdziła poczucie istnienia zaburzenia głosu u mężczyzn w wieku podeszłym, wskazując potrzebę szybkiej i trafnej diagnostyki klinicznej

Zastosowanie techniki szybkiego filmu w sekwencji cyfrowej (HSDI) w diagnostyce postaci klinicznej dysfonii u chorych z refluksem krtaniowo-gardłowym (LPR)

Wstęp: Choroba refluksowa przełyku (GERD) może powodować występowanie powikłań pozaprzełykowych, do których należy refluks krtaniowo-gardłowy (LPR). Jest on spowodowany zmianami morfologicznymi w krtani z powodu wstecznego zarzucania treści żołądkowej. LPR ma bardzo bogatą symptomatologię. Nie ma patognomonicznego obrazu krtani dla LPR. W diagnostyce LPR wykorzystywana jest subiektywna skala RSI, oceniająca symptomy w połączeniu z laryngoskopową oceną krtani w skali RFS. Cel: Celem pracy była diagnostyka postaci klinicznej dysfonii u chorych z LPR z wykorzystaniem techniki HSDI. Materiał i metody: Badaniem objęto grupę 72 pacjentów osiągających wynik > 13 w skali RSI i > 7 w skali RFS. Wyniki: Szczegółowa diagnostyka obrazowa techniką HSDI z wykorzystaniem kamery w trybie wysokiej szybkości (HS) i wysokiej rozdzielczości (HR) pozwoliła na precyzyjną obiektywną diagnostykę zaburzeń jakości głosu (dysfonii) o typie organicznym i czynnościowym o charakterze hiperfunkcjonalnym

Association between the Area of the Highest Flank Temperature and Concentrations of Reproductive Hormones during Pregnancy in Polish Konik Horses—A Preliminary Study

Determination of the pregnancy status is one of the most important factors for effective pregnancy management. Knowledge of the stage of pregnancy is important to interpret many of the reproductive hormones’ concentrations, including progesterone (P4), estrone sulfate (E1S), 17-ß estradiol (E2), and relaxin (REL). However, it is limited in wildlife or captive equids that cannot be handled. Reproductive hormones affect regional blood flow, the proliferation of tissues, and local metabolism intensity. Therefore, this preliminary study aimed to assess changes in thermal features of the abdomen lateral surface and concentrations of reproductive hormones in Polish native pregnant mares. The study was carried out on 14 non-pregnant and 26 pregnant Polish Konik mares during eleven months of pregnancy. Infrared thermography was conducted to image the lateral surface of mares’ abdomen (Px1) and flank area (Px2); P4, E1S, E2, and REL concentrations in serum were also determined. The evidence of the association between the area with the highest temperatures (Area of Tmax) and serum concentrations of P4 (the slope = 1.373; p = 0.9245) and REL (the slope = 1.342; p = 0.4324) were noted dependent across months of pregnancy. Measures of superficial body temperatures were found to change monthly, similarly to ambient temperatures, with no evidence of coincidence with changes in reproductive hormone concentrations. Individual thermal characteristics of the lateral surface of the abdomen differed between pregnant and non-pregnant mares in other periods. Differences in maximal and average temperature and Area of Tmax were observed from the sixth month of pregnancy, and those in minimal temperature were observed from the eighth month

Phoniatric, Audiological, Orodental and Speech Problems in a Boy with Cardio-Facio-Cutaneous Syndrome Type 3 (CFC 3) Due to a Pathogenic Variant in MAP2K1 - Case Study.

Cardio-facio-cutaneous syndrome 3 (CFC3) due to variants in MAP2K1 is a rare genetic disorder manifested mainly by short stature, facial dysmorphism, abnormalities of the cardiovascular system, skin changes, and intellectual disability. The aim of the study is the evaluation of the occurrence of pathological changes in the upper respiratory tract, orthodontic disorders, as well as voice, speech and hearing abnormalities in an 11-year-old boy with CFC3 syndrome. The lack of detailed diagnostics of speech, voice and hearing disorders, as well as the degree of their severity was an inspiration to undertake research in this field. Pathological changes in face, oral cavity, upper respiratory tract (nose, nasopharynx, larynx), and hearing organ, as well as voice and speech quality, were assessed in an 11-year-old boy with CFC3 syndrome. Pathologies of the upper respiratory tract (adenoid hypertrophy, narrowing of the nasal passages) and laryngeal asymmetry were found without significant changes in voice quality in the acoustic examination, except for the voice timbre change confirmed in narrowband spectrography. Complex audiological assessment confirmed the existence of bilateral sensorineural hearing loss. Speech pathology assessment revealed abnormalities in the structure of articulation organ, its decreased motor efficiency, imprecision, reduced coordination, as well as the presence of autistic features. Exome sequencing showed the heterozygous variant c.371C>T (p.Pro124Leu) in the MAP2K1 gene, previously described as pathogenic, thus supporting a causative relevance. Phoniatric, audiological, orodental and speech problems should be considered as features of cardio-facio-cutaneous syndrome type 3 (CFC 3) phenotype due to a pathogenic variant in MAP2K1