Expression of genes involved in heavy metal trafficking in plants exposed to salinity stress and elevated cd concentrations

Many areas intended for crop production suffer from the concomitant occurrence of heavy metal pollution and elevated salinity; therefore, halophytes seem to represent a promising perspective for the bioremediation of contaminated soils. In this study, the influence of Cd treatment (0.01–10.0 mM) and salinity stress (0.4 M NaCl) on the expression of genes involved in heavy metal uptake (irt2–iron-regulated protein 2, zip4–zinc-induced protein 4), vacuolar sequestration (abcc2–ATP-binding cassette 2, cax4–cation exchanger 2 pcs1–phytochelatin synthase 1) and translocation into aerial organs (hma4–heavy metal ATPase 4) were analyzed in a soil-grown semi-halophyte Mesembryanthemum crystallinum. The upregulation of irt2 expression induced by salinity was additionally enhanced by Cd treatment. Such changes were not observed for zip4. Stressor-induced alterations in abcc2, cax4, hma4 and pcs1 expression were most pronounced in the root tissue, and the expression of cax4, hma4 and pcs1 was upregulated in response to salinity and Cd. However, the cumulative effect of both stressors, similar to the one described for irt2, was observed only in the case of pcs1. The importance of salt stress in the irt2 expression regulation mechanism is proposed. To the best of our knowledge, this study is the first to report the combined effect of salinity and heavy metal stress on genes involved in heavy metal trafficking

The Pro12Ala Polymorphism of the Peroxisome Proliferator-Activated Receptor Gamma Gene Modifies the Association of Physical Activity and Body Mass Changes in Polish Women

Peroxisome proliferator-activated receptor γ is a key regulator of adipogenesis, responsible for fatty acid storage and maintaining energy balance in the human body. Studies on the functional importance of the PPARG Pro12Ala polymorphic variants indicated that the observed alleles may influence body mass measurements; however, obtained results were inconsistent. We have decided to check if body mass changes observed in physically active participants will be modulated by the PPARG Pro12Ala genotype. The genotype distribution of the PPARG Pro12Ala allele was examined in a group of 201 Polish women measured for selected body mass variables before and after the completion of a 12-week training program. The results of our experiment suggest that PPARG genotype can modulate training-induced body mass measurements changes: after completion of the training program, Pro12/Pro12 homozygotes were characterised by a greater decrease of body fat mass measurements in comparison with 12Ala allele carriers. These results indicate that the PPARG 12Ala variant may impair the training-induced positive effects on body mass measurements; however, the detailed mechanism of such interaction remained unclear and observed correlation between PPARG genotype and body mass differential effects should be interpreted with caution

"Stare" media w obliczu "nowych", "nowe" w obliczu "starych"

Praca recenzowana / peer-reviewed pape

Study of Radial Wall Jets from Ceiling Diffusers at Variable Air Volume

The knowledge of the air velocity distribution in the supply jets is essential when designing ventilation and air conditioning systems. In this study, we tested and analyzed the velocity distributions in the radial wall jets—these jets are commonly used in ventilated rooms. Tests included jets from two ceiling diffusers of different constructions, at three airflow rates. The mean air speed distributions were measured with a 16-channel hot-sphere anemometer both in the self-similarity zone and in the terminal zone. A specially developed method of converting the mean speed to mean velocity was used. The measurement results show that the spread coefficients of the jets from both diffusers were the same, but the positions of the virtual origin were different. Due to the friction of the jet with the ceiling and the transfer of momentum to the recirculating flows, the momentum flux in the self-similarity zone decreased by up to 50%. An improved method for calculating velocity distributions in radial wall jets was developed and validated. This method takes into account the decrease of momentum, non-zero position of the jet origin, and faster velocity decrease in the terminal zone. A reliable method of predicting air velocity distribution in radial wall jets (RWJs) from ceiling diffusers may allow to properly select the diffuser size, its location, and the range of flow rate changes. The design process for variable air volume systems can be facilitated