The Necessity of Close Contact Tracing in Combating COVID-19 Infection – A Systemic Study

Many contact tracing solutions developed by countries around the globe in containing the Covid-19 pandemic are in the area of location-based tracing, which does not enable them to identify close contacts accurately. As location-based tracing implementations continuous on, the results have not been as effective as intended. Thus, in providing some closure, this study will dissect the need for close contact tracing solutions for the pandemic by providing a comprehensive contact tracing characteristic framework (CCTCF) for Covid-19, which will help authorities toward better pandemic management. In this study, CCTCF for Covid-19 was constructed by applying several methods. Using Problem, Intervention, Comparison, Outcome (PICO) as the framework, methods conducted were: (1) Case study to analyze the contact tracing systems in 30 countries; (2) Systematic literature review (n=2056) regarding solutions’ elements, (3) Thematic analysis for characteristics framework development. A total of 25 items were obtained for CCTCF, along with valuable insights that necessitate close contact tracing for the pandemic. Results from CCTCF have also shown that the best contact tracing solution for Covid-19 is bi-directional human-to-human close contact tracing, which uses a retrospective approach and is able to identify the source as well as groups of infection using a personal area network (PAN). Doi: 10.28991/esj-2022-SPER-019 Full Text: PD

Confirmation of Skywalker Hoolock Gibbon (Hoolock tianxing) in Myanmar extends known geographic range of an endangered primate

Characterizing genetically distinct populations of primates is important for protecting biodiversity and effectively allocating conservation resources. Skywalker gibbons (Hoolock tianxing) were first described in 2017, with the only confirmed population consisting of 150 individuals in Mt. Gaoligong, Yunnan Province, China. Based on river geography, the distribution of the skywalker gibbon has been hypothesized to extend into Myanmar between the N’Mai Kha and Ayeyarwaddy Rivers to the west, and the Salween River (named the Thanlwin River in Myanmar and Nujiang River in China) to the east. We conducted acoustic point-count sampling surveys, collected noninvasive samples for molecular mitochondrial cytochrome b gene identification, and took photographs for morphological identification at six sites in Kachin State and three sites in Shan State to determine the presence of skywalker gibbons in predicted suitable forest areas in Myanmar. We also conducted 50 semistructured interviews with members of communities surrounding gibbon range forests to understand potential threats. In Kachin State, we audio-recorded 23 gibbon groups with group densities ranging between 0.57 and 3.6 group/km2. In Shan State, we audio-recorded 21 gibbon groups with group densities ranging between 0.134 and 1.0 group/km2. Based on genetic data obtained from skin and saliva samples, the gibbons were identified as skywalker gibbons (99.54–100% identity). Although these findings increase the species’ known population size and confirmed distribution, skywalker gibbons in Myanmar are threatened by local habitat loss, degradation, and fragmentation. Most of the skywalker gibbon population in Myanmar exists outside protected areas. Therefore, the IUCN Red List status of the skywalker gibbon should remain as Endangered

The emergence and diversification of a zoonotic pathogen from within the microbiota of intensively farmed pigs

The expansion and intensification of livestock production is predicted to promote the emergence of pathogens. As pathogens sometimes jump between species, this can affect the health of humans as well as livestock. Here, we investigate how livestock microbiota can act as a source of these emerging pathogens through analysis of Streptococcus suis, a ubiquitous component of the respiratory microbiota of pigs that is also a major cause of disease on pig farms and an important zoonotic pathogen. Combining molecular dating, phylogeography, and comparative genomic analyses of a large collection of isolates, we find that several pathogenic lineages of S. suis emerged in the 19th and 20th centuries, during an early period of growth in pig farming. These lineages have since spread between countries and continents, mirroring trade in live pigs. They are distinguished by the presence of three genomic islands with putative roles in metabolism and cell adhesion, and an ongoing reduction in genome size, which may reflect their recent shift to a more pathogenic ecology. Reconstructions of the evolutionary histories of these islands reveal constraints on pathogen emergence that could inform control strategies, with pathogenic lineages consistently emerging from one subpopulation of S. suis and acquiring genes through horizontal transfer from other pathogenic lineages. These results shed light on the capacity of the microbiota to rapidly evolve to exploit changes in their host population and suggest that the impact of changes in farming on the pathogenicity and zoonotic potential of S. suis is yet to be fully realized

The emergence and diversification of a zoonotic pathogen from within the microbiota of intensively farmed pigs

The expansion and intensification of livestock production is predicted to promote the emergence of pathogens. As pathogens sometimes jump between species, this can affect the health of humans as well as livestock. Here, we investigate how livestock microbiota can act as a source of these emerging pathogens through analysis of Streptococcus suis, a ubiquitous component of the respiratory microbiota of pigs that is also a major cause of disease on pig farms and an important zoonotic pathogen. Combining molecular dating, phylogeography, and comparative genomic analyses of a large collection of isolates, we find that several pathogenic lineages of S. suis emerged in the 19th and 20th centuries, during an early period of growth in pig farming. These lineages have since spread between countries and continents, mirroring trade in live pigs. They are distinguished by the presence of three genomic islands with putative roles in metabolism and cell adhesion, and an ongoing reduction in genome size, which may reflect their recent shift to a more pathogenic ecology. Reconstructions of the evolutionary histories of these islands reveal constraints on pathogen emergence that could inform control strategies, with pathogenic lineages consistently emerging from one subpopulation of S. suis and acquiring genes through horizontal transfer from other pathogenic lineages. These results shed light on the capacity of the microbiota to rapidly evolve to exploit changes in their host population and suggest that the impact of changes in farming on the pathogenicity and zoonotic potential of S. suis is yet to be fully realized.This work was primarily funded by an EU Horizon 2020 grant “PIGSs” (727966) and a ZELS BBSRC award “Myanmar Pigs Partnership (MPP)” (BB/L018934/1). G.G.R.M., E.L.M., and L.A.W. were supported by a Sir Henry Dale Fellowship to L.A.W. jointly funded by the Wellcome Trust and the Royal Society (109385/Z/15/Z). N.H. was supported by a Challenge grant from the Royal Society (CH16011) and an Isaac Newton Trust Research Grant [17.24(u)]. G.G.R.M. was also supported by a Research Fellowship at Newnham College. S.B. is supported by the Medical Research Council (MR/V032836/1). PIC North America provided part of the funds for the sequencing of the isolates from the USA. A.J.B. and M.M. were funded by Medical Research Council and Biotechnology and Biological Sciences Research Council studentships respectively, and M.M. was co-funded by the Raymond and Beverly Sackler Fund. We would like to acknowledge Susanna Williamson at the APHA for providing samples, Oscar Cabezón for sampling of the wild boar population in Spain, Mark O’Dea for access to sequence data from Australian isolates, the PIGSs and MPP consortiums for providing samples and helpful discussions, Julian Parkhill and John Welch for helpful discussions, and two anonymous reviewers for their valuable suggestions for improving the manuscript. This research was funded in whole or in part by the Wellcome Trust. For the purpose of Open Access, the author has applied a CC BY public copyright license to any Author Accepted Manuscript (AAM) version arising from this submission.info:eu-repo/semantics/publishedVersio

Crystalline Phase and Photocatalysis Activity of Electrochemically Anodized Titania Nanotubes

Titania (TiO2) nanotubes were developed by electrochemical anodization method. The crystalline phase of TiO2 nanotubes annealed at different temperatures was identified by X-ray diffraction. Anatase phase TiO2 started revealing at 300 ºC and pronounced at 500 ºC indicating the better crystallinity of TiO2 nanotubes. Photocatalysis activity of TiO2 nanotubes in methylene blue solution was also examined under UV light irradiation. High temperature annealed TiO2 nanotubes improved the photodegradation of methylene blue in aqueous solution

Effect of Chelating Agent on Surface Morphology and Electrochemical Properties of TiO2 Nanotube Arrays

Tatiana (TiO2) nanotubes were developed by electrochemical anodization method. Upon introducing the chelating agent (Na2EDTA) in the electrochemical growth solution, the changes in surface morphology and size (diameter and length), optical absorption and electrochemical energy levels of TiO2 nanotubes were determined by using Scanning Electron Microscopy (SEM), UV-Vis spectrophotometry with integrating sphere and cyclic voltammetry. In addition, organic dye (LEG4) was infiltrated into TiO2 nanotubes and the effect of Na2EDTA on the dye coverage and dye molecular levels were also investigated

Real Time Fire Detection System with Image Processing

Fire detection by image processing is a growing research in this era. With many methods used to detect fire out there, an accurate method to detect fire without false alarms has not been produced much. This is due to the fact that many methods used RGB colour mode for detection. In this paper, the main focus was on detecting the fire effectively using thermal video from a thermal camera while in the same time will alert the people if fire was detected and also observed the speed of the fire which will benefit enormously to the fire fighters. With this, it was hope that fire can be detected effectively while alerting the people and giving valuable information to the fire fighters to do their job more effectively

A Novel Approach on Covid-19 Contact Tracing – Utilization of Low Calibrated Transmission Power & Signal Captures in BLE

Covid-19 pandemic has compelled countries to conduct contact tracing vigorously in order to curb the highly infectious virus from further spread. In this context, Bluetooth Low Energy (BLE) has been broadly used, utilizing Received Signal Strength Indicator (RSSI) for Close Contact Identification (CCI). However, many of the available solutions are not able to adhere to the guidelines provided by Centers for Disease Control (CDC) and Prevention which are: (1) Distance requirement of within 6-feet (~2 meters) and (2) Minimum 15-minutes duration for CCI. In providing some closure to the gap, we proposed a novel approach of utilizing: (1) Low calibrated transmission power (Tx) and (2) Number of signal captures. Our proposed approach is to lowly calibrate Tx so that when distance is at 2 meters between users, number signal capture gets lower as the chipset’s smallest RSSI sensitivity value has been reached. In this paper, complete experimentation for Proof of Concept (POC) and Pilot test conducted are demonstrated. Results obtained shows that the accuracy for POC utilizing signal captures for 2 0.3 m distance is at: (1) 71.43% for 5 users and (2) 70.69% for 9 users. While so, accuracy for the Pilot test when considering CCI on individual case-basis is at 95% for 5 users

Willingness to use HIV pre-exposure prophylaxis among gay men, other men who have sex with men and transgender women in Myanmar

Introduction: HIV pre-exposure prophylaxis (PrEP) has emerged as a key component of contemporary HIV combination prevention strategies. To explore the local suitability of PrEP, country-specific acceptability studies are needed to inform potential PrEP implementation. In the context of Myanmar, in addition to resource constraints, HIV service access by gay men, other men who have sex with men, and transgender women (GMT) continues to be constrained by legislative and community stigma and marginalization. We aimed to determine PrEP acceptability among GMT in Myanmar and explore the factors associated with willingness to use PrEP. Methods: GMT were recruited in Yangon and Mandalay through local HIV prevention outreach programmes in November and December 2014. Quantitative surveys were administered by trained peer educators and collected data on demographics, sexual risk, testing history and PrEP acceptability. A modified six-item PrEP acceptability scale classified self-reported HIV undiagnosed GMT as willing to use PrEP. Multivariable logistic regression identified factors associated with willingness to use PrEP. Results: Among 434 HIV undiagnosed GMT, PrEP awareness was low (5%). PrEP acceptability was high, with 270 (62%) GMT classified as willing to use PrEP. GMT recruited in Mandalay (adjusted odds ratio (aOR) = 1.79; 95%CI = 1.05–3.03), who perceived themselves as likely to become HIV positive (aOR = 1.82; 95%CI = 1.10–3.02), who had more than one recent regular partner (aOR = 2.94; 95%CI = 1.41–6.14), no regular partners (aOR = 2.05; 95%CI = 1.10–3.67), more than five casual partners (aOR = 2.05; 95%CI = 1.06–3.99) or no casual partners (aOR = 2.25; 95%CI = 1.23–4.11) were more likely to be willing to use PrEP. The association between never or only occasionally using condoms with casual partners and willingness to use PrEP was marginally significant (aOR = 2.02; 95%CI = 1.00–4.10). GMT who reported concern about side effects and long-term use of PrEP were less likely (aOR = 0.35; 95%CI = 0.21–0.59) to be willing to use PrEP. Conclusions: This is the first study to assess PrEP acceptability in Myanmar. Findings suggest PrEP is an acceptable prevention option among GMT in Myanmar, providing they are not required to pay for it. Implementation/demonstration projects are needed to explore the feasibility and cost-effectiveness of PrEP as a prevention option for GMT in Myanmar