Alcohol Pattern Consumption Differently Affects the Efficiency of Macrophage Reverse Cholesterol Transport in Vivo

It has been well established that moderate alcohol consumption inversely correlates with cardiovascular morbidity and mortality, whereas binge alcohol drinking increases cardiovascular disease risk. The aim of this study was to assess in vivo the impact of different drinking patterns on reverse cholesterol transport (RCT); the atheroprotective process leading to the removal of excess cholesterol from the body. RCT was measured with a standardized, radioisotope-based technique in three groups of atherosclerosis-prone apolipoprotein E knock out mice: Placebo group, receiving water, which would mimic the abstainers; moderate group, receiving 0.8 g/kg alcohol/day for 28 days, which would mimic a moderate intake; binge group, receiving 0.8 g/kg alcohol/day for 5 days/week, followed by the administration of 2.8 g/kg alcohol/day for 2 days/week, which would mimic a heavy intake in a short period. Mice in the binge drinking group displayed an increase in total cholesterol, high density lipoprotein cholesterol (HDL-c) and non-HDL-c (all p < 0.0001 vs. placebo), and a significantly reduced elimination of fecal cholesterol. The moderate consumption did not lead to any changes in circulating lipids, but slightly improved cholesterol mobilization along the RCT pathway. Overall, our data confirm the importance of considering not only the total amount, but also the different consumption patterns to define the impact of alcohol on cardiovascular risk

MÓDULO 1: UTILIZAÇÃO DE EMBALAGENS TETRA PAK COMO ISOLANTE TÉRMICO DE HABITAÇÕES POPULARES DE MADEIRA

O trabalho tem o intuito de reutilizar embalagens do tipo Tetra Pak pós-consumo geradas na cidade de Alegrete/RS para a confecção de painéis térmicos para vedação vertical. Esse tipo de material é composto por seis camadas sobrepostas que permite que a embalagem apresente propriedades térmicas significativas. Por se tratar de uma embalagem descartável, de difícil processamento para a reciclagem, representa um produto de alto potencial em geração de resíduos. Embora apresentem comprovadamente boas características térmicas, as embalagens ainda são pouco exploradas na área da construção civil. Este projeto de extensão propõem a reutilização de embalagens do tipo “Tetra Pak” como “matéria prima” para a construção de mantas térmicas para painéis verticais. Tendo por objetivo melhorar a saúde pública por meio do aumento do conforto térmico, evitando a entrada de frio, chuva e calor das residências beneficiadas pelo projeto. A metodologia do projeto é dividida em cinco tópicos principais: coleta e higienização das embalagens Tetra Pak; preparação dos painéis térmicos; escolha das residências; aplicação dos painéis nas paredes internas das residências selecionadas e avaliação da satisfação do público alvo. Sendo o público alvo famílias de baixa renda que residam em edificações de madeira na cidade de Alegrete/RS

Application of fibre optic sensing systems to measure rotor blade structural dynamics

This paper compares two fibre optic sensing techniques for vibration characterisation: (a) optical fibre Bragg grating (FBG) strain gauges and (b) a novel direct fibre optic shape sensing (DFOSS) approach based on differential interferometric strain measurements between multiple fibres within the same fibre arrangement. Operational mode shapes and frequency measurements of an Airbus Helicopters H135 bearingless main rotor blade (5.1 m radius) were acquired during a series of ground vibration tests undertaken in a controlled laboratory environment. Data recorded by the fibre optic instrumentation systems were validated using commercially available accelerometers and compared against a baseline finite element model. Both fibre optic sensing systems proved capable of identifying the natural frequencies of the blade in the frequency range of interest (0–100 Hz). The data from the FBG sensors exhibited a dependency on their position relative to the neutral axes of the blade, which meant that full characterisation of the flapping and lagging modes required careful consideration of sensor location in the chordwise direction. The DFOSS system was able to identify all structural dynamics, despite being located on the neutral axis in the lagging direction, due to its sensitivity to angle changes, rather than strain, and its biaxial measurement capability. The DFOSS system also allowed the operational mode shapes of the blade to be determined directly, without the requirement for strain transfer from the blade to the sensor and without the requirement for a model of the underlying structure. The accuracy of obtained natural frequencies and operational mode shapes is assessed, demonstrating the potential of the use of both fibre optic sensing systems for determining blade structural dynamics

The SSDC Role in the LICIACube Mission: Data Management and the MATISSE Tool

Light Italian Cubesat for Imaging of Asteroids (LICIACube) is an Italian mission managed by the Italian Space Agency (ASI) and part of the NASA Double Asteroid Redirection Test (DART) planetary defense mission. Its main goals are to document the effects of the DART impact on Dimorphos, the secondary member of the (65803) Didymos binary asteroid system, characterizing the shape of the target body and performing dedicated scientific investigations on it. Within this framework, the mission Science Operations Center will be managed by the Space Science Data Center (ASI-SSDC), which will have the responsibility of processing, archiving, and disseminating the data acquired by the two LICIACube onboard cameras. In order to better accomplish this task, SSDC also plans to use and modify its scientific webtool Multi-purpose Advanced Tool for Instruments for the solar system Exploration (MATISSE), making it the primary tool for the LICIACube data analysis, thanks to its advanced capabilities for searching and visualizing data, particularly useful for the irregular shapes common to several small bodies

Bladesense – a novel approach for measuring dynamic helicopter rotor blade deformation

Technologies that allow accurate measurement of rotorblade dynamics can impact almost all areas of the rotorcraft sector; ranging from maintenance all the way to blade design. The BladeSense project initiated in 2016 aims to take a step in developing and demonstrating such a capability using novel fibre optic sensors that allow direct shape measurement. In this article the authors summarise key project activities in modelling and simulation, instrumentation development and ground testing. The engineering approach and associated challenges and achievements in each of these disciplines are discussed albeit briefly. This ranges from the use of computational aerodynamics and structural modelling to predict blade dynamics to the development of direct fibre optic shape sensing that allows measurements above 1kHz over numerous positions on the blade. Moreover, the development of the prototype onboard system that overcomes the challenge of transferring data between the rotating main rotor to the fixed fuselage frames is also discussed

Engineering an Environment for the Study of Fibrosis: A 3D Human Muscle Model with Endothelium Specificity and Endomysium

The integration of vascular structures into in vitro cultured tissues provides realistic models of complex tissue-vascular interactions. Despite the incidence and impact of muscle-wasting disorders, advanced in vitro systems are still far from recapitulating the environmental complexity of skeletal muscle. Our model comprises differentiated human muscle fibers enveloped by a sheath of human muscle-derived fibroblasts and supported by a vascular network with mural-like cells. Here, we demonstrate the induction of muscle-specific endothelium and the self-organization of endomysial muscle fibroblasts mediated by endothelial cells. We use this model to mimic the fibrotic environment characterizing muscular dystrophies and to highlight key signatures of fibrosis that are neglected or underestimated in traditional 2D monocultures. Overall, this vascularized meso-scale cellular construct finely recapitulates the human skeletal muscle environment and provides an advanced solution for in vitro studies of muscle physiology and pathology. Bersini et al. demonstrate the generation of a mesoscale model of the human muscle environment and prove its application for the study of fibrosis. This engineered muscle environment promotes the organ-specific differentiation of endothelial cells and the self-assembly of myofibers spontaneously wrapped by a continuous endomysium-like structure

Early stability and late random tumor progression of a HER2-positive primary breast cancer patient-derived xenograft

We established patient-derived xenografts (PDX) from human primary breast cancers and studied whether stability or progressive events occurred during long-term in vivo passages (up to 4&nbsp;years) in severely immunodeficient mice. While most PDX showed stable biomarker expression and growth phenotype, a HER2-positive PDX (PDX-BRB4) originated a subline (out of 6 studied in parallel) that progressively acquired a significantly increased tumor growth rate, resistance to cell senescence of in vitro cultures, increased stem cell marker expression and high lung metastatic ability, along with a strong decrease of BCL2 expression. RNAseq analysis of the progressed subline showed that BCL2 was connected to three main hub genes also down-regulated (CDKN2A, STAT5A and WT1). Gene expression of progressed subline suggested a partial epithelial-to-mesenchymal transition. PDX-BRB4 with its progressed subline is a preclinical model mirroring the clinical paradox of high level-BCL2 as a good prognostic factor in breast cancer. Sequential in vivo passages of PDX-BRB4 chronically treated with trastuzumab developed progressive loss of sensitivity to trastuzumab while HER2 expression and sensitivity to the pan-HER tyrosine kinase inhibitor neratinib were maintained. Long-term PDX studies, even though demanding, can originate new preclinical models, suitable to investigate the mechanisms of breast cancer progression and new therapeutic approaches

VADER: Probing the Dark Side of Dimorphos with LICIACube LUKE

The ASI cubesat LICIACube has been part of the first planetary defense mission DART, having among its scopes to complement the DRACO images to better constrain the Dimorphos shape. LICIACube had two different cameras, LEIA and LUKE, and to accomplish its goal, it exploited the unique possibility of acquiring images of the Dimorphos hemisphere not seen by DART from a vantage point of view, in both time and space. This work is indeed aimed at constraining the tridimensional shape of Dimorphos, starting from both LUKE images of the nonimpacted hemisphere of Dimorphos and the results obtained by DART looking at the impacted hemisphere. To this aim, we developed a semiautomatic Computer Vision algorithm, named VADER, able to identify objects of interest on the basis of physical characteristics, subsequently used as input to retrieve the shape of the ellipse projected in the LUKE images analyzed. Thanks to this shape, we then extracted information about the Dimorphos ellipsoid by applying a series of quantitative geometric considerations. Although the solution space coming from this analysis includes the triaxial ellipsoid found by using DART images, we cannot discard the possibility that Dimorphos has a more elongated shape, more similar to what is expected from previous theories and observations. The result of our work seems therefore to emphasize the unique value of the LICIACube mission and its images, making even clearer the need of having different points of view to accurately define the shape of an asteroid.This work was supported by the Italian Space Agency (ASI) within the LICIACube project (ASI-INAF agreement AC No. 2019-31-HH.0) and by the DART mission, NASA contract 80MSFC20D0004

The Dimorphos ejecta plume properties revealed by LICIACube

The Double Asteroid Redirection Test (DART) had an impact with Dimorphos (a satellite of the asteroid Didymos) on 26 September 20221. Ground-based observations showed that the Didymos system brightened by a factor of 8.3 after the impact because of ejecta, returning to the pre-impact brightness 23.7 days afterwards2. Hubble Space Telescope observations made from 15 minutes after impact to 18.5 days after, with a spatial resolution of 2.1 kilometres per pixel, showed a complex evolution of the ejecta3, consistent with other asteroid impact events. The momentum enhancement factor, determined using the measured binary period change4, ranges between 2.2 and 4.9, depending on the assumptions about the mass and density of Dimorphos5. Here we report observations from the LUKE and LEIA instruments on the LICIACube cube satellite, which was deployed 15 days in advance of the impact of DART. Data were taken from 71 seconds before the impact until 320 seconds afterwards. The ejecta plume was a cone with an aperture angle of 140 ± 4 degrees. The inner region of the plume was blue, becoming redder with increasing distance from Dimorphos. The ejecta plume exhibited a complex and inhomogeneous structure, characterized by filaments, dust grains and single or clustered boulders. The ejecta velocities ranged from a few tens of metres per second to about 500 metres per second.This work was supported by the Italian Space Agency (ASI) in the LICIACube project (ASI-INAF agreement AC no. 2019-31-HH.0) and by the DART mission, NASA contract 80MSFC20D0004. M.Z. acknowledges Caltech and the Jet Propulsion Laboratory for granting the University of Bologna a licence to an executable version of MONTE Project Edition software. M.Z. is grateful to D. Lubey, M. Smith, D. Mages, C. Hollenberg and S. Bhaskaran of NASA/JPL for the discussions and suggestions regarding the operational navigation of LICIACube. G.P. acknowledges financial support from the Centre national d’études spatiales (CNES, France). A.C.B. acknowledges funding by the NEO-MAPP project (grant agreement 870377, EC H2020-SPACE-2019) and by the Ministerio de Ciencia Innovación (PGC 2018) RTI2018-099464-B-I00. F.F. acknowledges funding from the Swiss National Science Foundation (SNSF) Ambizione (grant no. 193346). J.-Y.L. acknowledges the support from the NASA DART Participating Scientist Program (grant no. 80NSSC21K1131). S.D.R. and M.J. acknowledge support from the Swiss National Science Foundation (project no. 200021_207359)