Use of spectroscopic indicators for the monitoring of bromate generation in ozonated wastewater containing variable concentrations of bromide

Time-resolved monitoring of bromate and other by-products formed into effluents treated with ozone or advanced oxidation processes in wastewater treatment plants (WWTPs) is time-consuming and expensive. This study examined whether concentrations of bromate formed in wastewater after ozonation in the presence of widely varying bromide levels (from ca. 0.7–21.2 mg/L) can be quantified based on measurements of changes in optical properties (differential UV absorbance (ΔUVA), spectral slopes, total or regional fluorescence) of the ozonated samples. Batch ozonation was carried out using a secondary effluent produced at a major wastewater treatment plant located in the Metropolitan Seattle Area. The tests involved raw and bromide-spiked samples treated with ozone doses from 0.1 to 1 mg O3/mg DOC. Measurements of the absorbance at 254 nm (UVA254), fluorescence and bromate concentrations were performed on the treated samples. In the ozonated wastewater the concentration of bromate increased approximately linearly, from 10 mg/L) tended to inhibit the generation of bromate. Relative reduction of UVA254 and total fluorescence (TF) were found to be good predictors of bromate generation. Specifically, exponential curves could adequately fit the non-linear relationships found to exist between the concentrations of bromate and the relative reductions of the UV254 and TF, for any initial bromide concentrations used in this study. Little formation of bromate was found to occur for reduction ranges for UVA254 and TF of 30–40% and 70–80% respectively. Conversely, rapid increases in bromate generation were observed when the decrease of UVA254 or TF exceeded these threshold values

Analysis of the emergent climate change mitigation technologies

A climate change mitigation refers to efforts to reduce or prevent emission of greenhouse gases. Mitigation can mean using new technologies and renewable energies, making older equipment more energy efficient, or changing management practices or consumer behavior. The mitigation technologies are able to reduce or absorb the greenhouse gases (GHG) and, in particular, the CO2 present in the atmosphere. The CO2 is a persistent atmospheric gas. It seems increasingly likely that concentrations of CO2 and other greenhouse gases in the atmosphere will overshoot the 450 ppm CO2 target, widely seen as the upper limit of concentrations consistent with limiting the increase in global mean temperature from pre-industrial levels to around 2◦C. In order to stay well below to the 2◦C temperature thus compared to the pre-industrial level as required to the Paris Agreement it is necessary that in the future we will obtain a low (or better zero) emissions and it is also necessary that we will absorb a quantity of CO2 from the atmosphere, by 2070, equal to 10 Gt/y. In order to obtain this last point, so in order to absorb an amount of CO2 equal to about 10 Gt/y, it is necessary the implementation of the negative emission technologies. The negative emission technologies are technologies able to absorb the CO2 from the atmosphere. The aim of this work is to perform a detailed overview of the main mitigation technologies possibilities currently developed and, in particular, an analysis of an emergent negative emission technology: the microalgae massive cultivation for CO2 biofixation

Reaction of the NAD(P)H:flavin oxidoreductase from Escherichia coli with NADPH and riboflavin: identification of intermediates.

International audienceFlavin reductase catalyzes the reduction of free flavins by NAD(P)H. As isolated, Escherichia coli flavin reductase does not contain any flavin prosthetic group but accommodates both the reduced pyridine nucleotide and the flavin substrate in a ternary complex prior to oxidoreduction. The reduction of riboflavin by NADPH catalyzed by flavin reductase has been studied by static and rapid kinetics absorption spectroscopies. Static absorption spectroscopy experiments revealed that, in the presence of riboflavin and reduced pyridine nucleotide, flavin reductase stabilizes, although to a small extent, a charge-transfer complex of NADP+ and reduced riboflavin. In addition, reduction of riboflavin was found to be essentially irreversible. Rapid kinetics absorption spectroscopy studies demonstrated the occurrence of two intermediates with long-wavelength absorption during the catalytic cycle. Such intermediate species exhibit spectroscopic properties similar to those of charge-transfer complexes of oxidized flavin and NAD(P)H, and reduced flavin and NAD(P)+, respectively, which have been identified as intermediates during the reaction of flavoenzymes of the ferredoxin-NADP+ reductase family. Thus, a minimal kinetic scheme for the reaction of flavin reductase with NADPH and riboflavin can be proposed. After formation of the Michaelis complex of flavin reductase with NADPH and riboflavin, a first intermediate, identified as a charge-transfer complex of NADPH and riboflavin, is formed. It is followed by a second charge-transfer intermediate of enzyme-bound NADP+ and reduced riboflavin. The latter decays, yielding the Michaelis complex of flavin reductase with NADP+ and reduced riboflavin, which then dissociates to complete the reaction. These results support the initial hypothesis of a structural similarity between flavin reductase and the enzymes of the ferredoxin-NADP+ reductase family and extend it at a functional level

Traditional free-weight vs. variable resistance training applied to elite young soccer players during a short preseason: effects on strength, speed, and power performance

Maximizing the neuromuscular capacities of players is a critical challenge during short soccer preseasons. This study compared the effects of two strength-power training regimes, on the strength, speed, and power performance of elite young soccer players during a 4-week preseason. Twenty-five under-20 players from the same club were pair-matched in two training groups as follows: traditional training group (TTG) (n=13), athletes performed half-squat (HS) and jump-squat (JS) exercises as traditionally prescribed; and EB group (EBG) (n=12), athletes performed HS and JS with EB attached to the barbell. Vertical jump height, 20-m sprint velocity, change-of-direction (COD) speed, HS and JS power, and one-repetition maximum (1RM) in the HS were assessed pre, post 2-week, and post 4-week of training. An ANOVA two-way with repeated measures was used to assess the effects of both training protocols over the experimental period. Both strategies were effective for significantly improving HS and JS power (effect sizes [ES] 1.00 - 1.77), HS 1RM (ES = 1.68 and 1.51 for TTG and EBG, respectively), vertical jumping ability (ES 0.37 - 0.65), and COD speed (ES = 0.81 and 0.39 for TTG and EBG, respectively), when comparing pre- and post-measures. In contrast, both TTG and EBG failed to increase 20-m sprint velocity (ES ranging between -0.54 and 0.23). In conclusion, both training schemes were able to improve the strength and power performance, but not the sprint capacity of young soccer players. To accelerate strength gains over very-short time periods (i.e., 2-week), variable resistance training may be advantageous. Conversely, to optimize power adaptations in ballistic exercises across a similar time period, traditional FW training may be preferred

PRELIMINARY TAKE-OFF ANALYSIS AND SIMULATION FOR A PRANDTLPLANE COMMERCIAL AIRCRAFT

The paper describes the take-off performances and characteristics of an unconventional aircraft, called PrandtlPlane. The PrandtlPlane has a box-wing architecture, founded on the “Best Wing System” concept due to L. Prandtl, that minimizes the induced drag once wingspan and lift are given. This configuration has the potential to be a more efficient alternative to conventional tube-and-wing aircraft, and it is under investigation in the framework of the PARSIFAL project, funded by the European Union in the Horizon 2020 program. A numerical simulation tool for the take-off dynamics of the aircraft, based on the non-linear equations of motion, has been developed in order to evaluate the performance of the aircraft in take-off condition. The VLM solver has been integrated into this tool in order to evaluate the aerodynamic performance of the aircraft in ground effect at each moment of the manoeuvre. The same assessments have been made for a conventional tube-and-wing reference aircraft, with the aim of conducting a performance comparison with the reference PrandtlPlane. The preliminary results obtained show the aerodynamic and aeromechanical advantages of the reference PrandtlPlane, in terms of runway length and passenger comfort

Structural and functional diversity of ferredoxin-NADP+ reductases

Although all ferredoxin-NADP+ reductases (FNRs) catalyze the same reaction, i.e. the transfer of reducing equivalents between NADP(H) and ferredoxin, they belong to two unrelated families of proteins: the plant-type and the glutathione reductase-type of FNRs. Aim of this review is to provide a general classification scheme for these enzymes, to be used as a framework for the comparison of their properties. Furthermore, we report on some recent findings, which significantly increased the understanding of the structure–function relationships of FNRs, i.e. the ability of adrenodoxin reductase and its homologs to catalyze the oxidation of NADP+ to its 4-oxo derivative, and the properties of plant-type FNRs from non-photosynthetic organisms. Plant-type FNRs from bacteria and Apicomplexan parasites provide examples of novel ways of FAD- and NADP(H)-binding. The recent characterization of an FNR from Plasmodium falciparum brings these enzymes into the field of drug design

Evaluation of green coffee-roasting biogas with modeling valorization of possible solutions

According to the European Union Directive 2009/28/EC, the goals of obtaining 20% of all energy requirements from renewable sources and a 20% reduction in primary energy use must be fulfilled by 2020. In this work, an evaluation was performed, from the environmental and energy point of view, of anaerobic digestion as a valid solution for the treatment of the byproducts obtained from the coffee-roasting process. In particular, thermophilic anaerobic digestion tests were carried out. Output values from the laboratory were used as input for the MCBioCH4 model to evaluate the produced flow of biogas and biomethane and two different biogas valorization alternatives, namely, the traditional exploitation of biogas for heat/energy production and biomethane conversion. The results of the preliminary simulation showed that a full-scale implementation of the coffee waste biogas production process is technically feasible and environmentally sustainable. Furthermore, the performed analysis validates a general methodology for energy production compatibility planning

Diversity Climate Survey Results: Changing Institutional Culture

Purpose: To gather and analyze data at two points in time on perceptions of institutional values connected to a wide range of diversity issues. This study gauges student, faculty, and staff views on institutional support of diversity with results guiding future inclusion and training efforts within the organization. Presented at the AAMC (Association of American Colleges) Annual Meeting, RIME (Research in Medical Education) Program, November 2008

Debonding of adhesive joints by means of microwave and induction heating processes

In this work, an innovative technique for adhesive joint separation that combines the use of a hybrid-modified adhesive with microwave (MW) or induction heating (IH) [1-3] processes is presented. Graphene nanoplatelets (GnPs) and iron oxide particles were used to modify a thermoplastic adhesive, polyolefin hot-melt adhesive by mean of a twin-screw extruder. This thermoplastic adhesive, already used for bonding automotive applications, was modified with both iron oxide and GnPs in order to enhance the electrical properties and the sensitivity to MW and IH. The mechanical and electrical properties together with the sensitivity of the modified adhesives to microwave or induction heating processes are investigated. Single Lap Joint (SLJ) specimens were used to evaluate the mechanical properties of the pristine and the modified adhesive. The mechanical tests illustrate that the maximum loads of modified adhesives decrease slightly. Tests conducted with microwave and induction heating processes showed that these two systems are able to melt the modified adhesive. Thus, the separation of bonded joints is possible with both systems. The temperature increase of the induction heating system is found to be more rapid than the microwaves but the latter system is energetically more efficient. Scanning Electron Microscope (SEM) was used to measure the particle distribution and to evaluate the differences between the manual mixed mode and the tween extruder system as preliminary analysis

Climate Justice in the City: Mapping Heat-Related Risk for Climate Change Mitigation of the Urban and Peri-Urban Area of Padua (Italy)

The mitigation of urban heat islands (UHIs) is crucial for promoting the sustainable development of urban areas. Geographic information systems (GISs) together with satellite-derived data are powerful tools for investigating the spatiotemporal distribution of UHIs. Depending on the availability of data and the geographic scale of the analysis, different methodologies can be adopted. Here, we show a complete open source GIS-based methodology based on satellite-driven data for investigating and mapping the impact of the UHI on the heat-related elderly risk (HERI) in the Functional Urban Area of Padua. Thermal anomalies in the territory were mapped by modelling satellite data from Sentinel-3. After a socio-demographic analysis, the HERI was mapped according to five levels of risk. The highest vulnerability levels were localised within the urban area and in three municipalities near Padua, which represent about 20% of the entire territory investigated. In these municipalities, a percentage of elderly people over 20%, a thermal anomaly over 2.4 °C, and a HERI over 0.65 were found. Based on these outputs, it is possible to define nature-based solutions for reducing the UHI phenomenon and promote a sustainable development of cities. Stakeholders can use the results of these investigations to define climate and environmental policies