26 research outputs found
Histopathological and immunohistochemical findings of swine with spontaneous influenza A infection in Brazil, 2009-2010
Uso do plasma suíno ultrafiltrado na recuperação de leitões com sinais clínicos de circovirose
Paroxysmal Nocturnal Hemoglobinuria (Pnh) : Brain Mri Ischemic Lesions in Neurologically Asymtomatic Patients
This study investigated for the first time brain ischemic involvement in 19 consecutive neurologically asymptomatic PNH patients by non-enhanced cerebral MRI, and by intracranial arterial and venous angio-MRI. Eleven cases (58%, 7 aged 5 mm, and 5 cases a score >4 by the age-related white matter changes (ARWMC) scale. Compared with age and sex-matched controls (1:2 ratio), patients showed an increased frequency of periventricular WM vascular degeneration (32% versus 5.2%, p = 0.04) and of severe lesions (ARWMC scale score >4) (26% versus 2.6%, p = 0.05), and a higher overall ARWMC scale score (3.5 \uc2\ub1 1.07 versus 2.0 \uc2\ub1 0.8, mean \uc2\ub1 SD, p < 0.0001). Notably, vascular abnormalities suspected for prior partial venous thrombosis, were observed in PNH cases only. MRI lesions were not related to blood counts, hemolytic markers, clone size, disease duration, and therapy with eculizumab. Neurological examination was unremarkable in all patients but one (Parkinson disease). Psychiatric assessment revealed a case of generalized anxiety disorder, 1 bipolar disorder type 2, and 1 adjustment disorder. In conclusion, brain MRI may be useful at diagnosis and during the course of the disease to explore subclinical neurological involvement
Estratégia para erradicação de focos da Doença de Aujeszky em suínos no Estado de São Paulo
Development and bloom in hybrids of wild passion fruit cultivated in different types of pots and shading levels
Trilhas e seu papel ecológico: o que temos aprendido e quais as perspectivas para a restauração de ecossistemas?
Whole-genome sequencing reveals host factors underlying critical COVID-19
Critical COVID-19 is caused by immune-mediated inflammatory lung injury. Host genetic variation influences the development of illness requiring critical care1 or hospitalization2,3,4 after infection with SARS-CoV-2. The GenOMICC (Genetics of Mortality in Critical Care) study enables the comparison of genomes from individuals who are critically ill with those of population controls to find underlying disease mechanisms. Here we use whole-genome sequencing in 7,491 critically ill individuals compared with 48,400 controls to discover and replicate 23 independent variants that significantly predispose to critical COVID-19. We identify 16 new independent associations, including variants within genes that are involved in interferon signalling (IL10RB and PLSCR1), leucocyte differentiation (BCL11A) and blood-type antigen secretor status (FUT2). Using transcriptome-wide association and colocalization to infer the effect of gene expression on disease severity, we find evidence that implicates multiple genes—including reduced expression of a membrane flippase (ATP11A), and increased expression of a mucin (MUC1)—in critical disease. Mendelian randomization provides evidence in support of causal roles for myeloid cell adhesion molecules (SELE, ICAM5 and CD209) and the coagulation factor F8, all of which are potentially druggable targets. Our results are broadly consistent with a multi-component model of COVID-19 pathophysiology, in which at least two distinct mechanisms can predispose to life-threatening disease: failure to control viral replication; or an enhanced tendency towards pulmonary inflammation and intravascular coagulation. We show that comparison between cases of critical illness and population controls is highly efficient for the detection of therapeutically relevant mechanisms of disease
Genetic Structure And Phenotypic Variation In Wild Populations Of The Medicinal Tetraploid Species Bromelia Antiacantha (bromeliaceae)
Premise of the study: The patterns of genetic structure in plant populations are mainly related to the species life history and breeding system, and knowledge of these patterns is necessary for the management, use, and conservation of biological diversity. Polyploidy is considered an important mode of evolution in plants, but few studies have evaluated genetic structure of polyploid populations. We studied the patterns of genetic structure and morphological variation of Bromelia antiacantha (Bromeliaceae) populations, a polyploid terrestrial species.Methods: Microsatellite markers and morphological analyses were used to explore patterns of genetic and morphological diversity in wild populations of B. antiacantha.Key results: The results of our simple-sequence repeat analyses supported that B. antiacantha is a polyploid species. The inbreeding coefficients were high and significant in all populations (F IS = 0.431), indicating homozygote excess. Bromelia antiacantha showed high levels of genetic differentiation among populations (F ST = 0.224) and therefore was highly structured. High morphological variation was observed in fruit phenotypic traits in the populations studied.Conclusions: The levels of genetic diversity and the pattern of the population ' s structure may be related to the low recruitment of seeds, clonal reproduction, and the population ' s colonization history. 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Characterization Of The Inclusion Complex Ropivacaine: β-cyclodextrin [caracterização Do Complexo De Inclusão Ropivacaína: β-ciclodextrina]
Ropivacaine (RVC) is a widely used local anesthetic. The complexation of RVC with β-cyclodextrin (β-CD) is of great interest for the development of more efficient local anesthetic formulations. The present work focuses on the characterization of the RVC:β-CD complex by nuclear magnetic resonance (NMR). The stoichiometry of the complex is 1:2 RVC:β-CD. DOSY-NMR shows that the association constant is 55.5 M-1. Longitudinal relaxation time results show that RVC changes its mobility in the presence of β-CD. This study is focused on the physicochemical characterization of inclusion complexes that are potentials options for pain treatment.30512031207Covino, B.G., Vassalo, H.G., (1985) Anestésicos locais: Mecanismos de ação e uso clínico, , Colina: Rio de JaneiroCourtney, K.R., Strichartz, G.R., (1987) Local Anesthetics, Handbook of Experimental Pharmacology, , Springer-Verlag: BerlinGupta, S.P., (1991) Chem. Rev, 91, p. 1109Kuzma, P.J., Kline, M.D., Calkins, M.D., Staats, P.S., (1997) Reg. Anesth, 22, p. 543McClure, J.H., (1996) Brit. J. Anaesth, 76, p. 300Cederholm, I., (1997) Drug Saf, 16, p. 391Knudsen, K., Suurkula, M.B., Blomberg, S., Sjovall, J., Edvardsson, N., (1997) Br. J. Anesth, 78, p. 507Emanuelsson, B.M., Person, J., Sadin, S., Alm, C., Gustafsson, L.L., (1997) Ther. Drug Monit, 19, p. 126O'Keeffe, N.J., Healy, T.E.J., (1999) Pharmacol. Ther, 84, p. 233Bennett, G., Deer, T., Stuart, D.P., Rauck, R., Yaksh, T., Hassenbusch, S.J., (2000) J. Pain Symp. Manag, 20, p. 44Whiteside, J.B., Wildsmith, J.A.W., (2001) Br. J. Anesth, 87, p. 27de Araújo, D.R., Pinto, L.M.A., Braga, A.F.A., de Paula, E., (2003) Rev. Bras. Anestesiol, 53, p. 663de Araújo, D.R., Fraceto, L.F., Braga, A.F.A., de Paula, E., (2005) Rev. Bras. Anestesiol, 55, p. 316Loftsson, T., Masson, M., (2001) Int. J. Pharm, 225, p. 15McCormack, B., Gregoriadis, G., (1998) Int. J. Pharm, 162, p. 59Matioli, G.Ciclodextrinas e suas aplicações em: alimentos, fármacos, cosméticos, agricultura, biotecnologia, química analítica e produtos gerais, Ed. Eduem: Maringá, 2000Irie, T., Uekama, K., (1997) J. Pharm. Sci, 86, p. 147Pinto, L.M.A., de Jesus, M.B., de Paula, E., Lino, A.C.S., Alderetec, J.B., Duarte, H.A., Takahata, Y., (2004) J. Mol. Struct. (Theochem), 678, p. 63Pinto, L.M.A., Fraceto, L.F., Santana, M.H., Pertinhez, T.A., Oyama-Junior, S., de Paula, E., (2005) J. Pharm. Biomed. Anal, 39, p. 956Kopecký, F., Vojteková, M., Kaclik, P., Demko, M., Bieliková, Z., (2004) J. Pharm. Pharmacol, 56, p. 581Etos, S., Noda, H., Noda, A., (1994) J. Chromatogr., B: Anal. Technol. Biomed. Life Sci, 19, p. 385Loukas, Y.L., Vraka, V., Gregoriadis, G., (1998) Int. J. Pharm, 162, p. 137Ivanov, P.M., Salvatierra, D., Jaime, C., (1996) J. Org. Chem, 61, p. 7012Laverde Jr., A., da Conceição, G.J.A., Queiroz, S.C.N., Fujiwara, F.Y., Marsaioli, A.J., (2002) Magn. Reson. Chem, 40, p. 433Johnson Jr., C.S., (1999) Prog. Nucl. Magn. Reson. Spectrosc, 34, p. 203Xiliang, G., Yu, Y., Guoyan, Z., Guomei, Z., Jianbin, C., Shaomin, S., (2003) Spectrochim. Acta, Part A, 59, p. 3379Bratu, I., Gavira-Vallejo, J.M., Hernanz, A., (2005) Biopolymers, 77, p. 361Ikeda, Y., Hirayama, F., Arima, H., Uekama, K., Yoshitake, Y., Harano, K., (2004) J. Pharm. Sci, 93, p. 1659Zouvelekis, D., Yannakopoulou, K., Mavridis, I.M., Antoniadou-Vyza, A., (2002) Carbohydr. Res, 337, p. 1387Munoz-Botella, S., Martin, M.A., Del Castillo, B., Menendez, J.C., Vazquez, L., Lerner, D.A., (1996) J. Pharm. Biomed. Anal, 14, p. 909Garcia-Rio, L., Hervés, P., Leis, J.R., Mejuto, J.C., Pérez-Juste, J., Rodriguez-Dafonte, P., (2006) Org. Biomol. Chem, 4, p. 1038Abou-Zeid, O.K., (2005) Spectrochim. Acta, Part A, 62, p. 245Pascal, B., Maud, G., Georges, D., Adelin, A., Valery, B., Bruno, P., Didier, C., Brigitte, E., (2005) J. Pharm. Pharmaceut. Sci, 8, p. 164Morris, K.F., Johnson Jr., C.S., (1992) J. Am. Chem. Soc, 114, p. 3139Morris, K.F., Johnson Jr., C.S., (1993) J. Am. Chem. Soc, 115, p. 4291Dollo, G., Le Corre, P., Chevanne, F., Le Verge, R., (1996) Int. J. Pharm, 136, p. 165Fraceto, L.F., Spisni, A., Schreier, S., de Paula, E., (2005) Biophys. Chem, 115, p. 11Fraceto, L.F., Pinto, L.M.A., Franzoni, L., Braga, A.A.C., Spisni, A., Schreier, S., de Paula, E., (2002) Biophys. Chem, 99, p. 22