Protein Biochemistry

In protein biochemistry, biochemists specially study proteins as complex polymers. Protein biochemistry is the study of the chemical structure of proteins, including individual amino acids, their linkage as well as physical structure of proteins. Proteins, especially globular proteins, are three dimensional in nature. Proper function of globular proteins is tightly related to their three-dimensional structures (Structure-Function Relationship). Their complex and diverse structure offer them a high degree of functional diversity.Structure-function relationship is one of important area studied in Protein Biochemistry.The chemical reactions which involve proteins are also of interest in Protein Biochemistry. These reactions are usually mediated by enzymes.Enzymes are molecular workers which are used for performing specific reactions in pharmaceutical, medical and food industries. They are applied for production (for example amylase for production of alcohols), elimination (for example catalase for elimination of hydrogen peroxide) and transformation (for example alcohol dehydrogenase for transformation of alcohol to aldehyde) of materials or used as food preservative (lysozyme). 

The Unfolded Protein Response in Relation to Mitochondrial Biogenesis in Skeletal Muscle Cells

Mitochondrial biogenesis involves nuclear- and mitochondrial-derived proteins to be integrated into functional organelles. Muscle development and chronic exercise are two physiological stimuli that trigger the production of mitochondrial components to produce more mitochondria. However, the synthesis of new proteins can induce cellular stress. Thus, the unfolded protein response (UPR), which takes place in the mitochondria or the endoplasmic reticulum, ensures correct protein handling. Whether the UPR must precede mitochondrial biogenesis is unknown. We used two models of mitochondrial biogenesis, skeletal muscle differentiation and chronic exercise of muscle cells in culture, and examined UPR activation. We partially inhibited one branch of the UPR involving the protein CHOP, with either a drug (TUDCA) or gene knockdown. Our results indicate that mitochondrial biogenesis occurs independently of stress-induced CHOP, and reducing ER stress may further augment mitochondrial content

QUANTITATIVE EVALUATION OF MEDICAL RECORD DOCUMENTATION IN IMAM REZA HOSPITAL, MASHHAD, IRAN

Introduction: Diabetes is the most common endocrine disease. Given the importance of medical record documentation for diabetic patients and its significant impact on accurate treatment process, as well as early diagnosis and treatment of acute and chronic complications, this study aimed to qualitatively evaluate medical record documentation of diabetic patients. Methods: This descriptive and cross-sectional study was conducted on all medical records of diabetic patients (1200 cases) in the comprehensive Diabetes Center of Imam Reza Hospital. A checklist was prepared according to the main sectors and their sub-data elements to conduct a qualitative evaluation on documentation of medical records of diabetic patients.  Descriptive statistics were used to report the results. Results: In this study, 1200 (710 women and 490 men) cases were evaluated. Mean documentation of main sectors of diabetic patients’ records were as follows: 49% demographic characteristics, 14% patient referral, 4% diagnosis, 50% lab tests, 25% diabetes medications,13% nephropathy screening test, 10% diabetic neuropathy, 41% specialty and subspecialty consultations and internal medicine physicians visits did not complete for all the patients. Conclusion: According to the results of this study, qualitative evaluation of medical record documentation of diabetic patients Showed poor documentation in this regard. It is suggested that results of this study be accessible to physicians of healthcare centers to take a positive step toward improved documentation of medical records. In addition, it seems necessary to modify diabetic medical records

Fracture Resistance of Premolars Restored by Various Types and Placement Techniques of Resin Composites

To verify the fracture resistance of premolars with mesioocclusodistal preparations restored by different resin composites and placement techniques. Sixty premolars were randomly divided into two groups based on type of composite resin: Filtek P60 or Nulite F, and then each group was separated into three subgroups: bulk, centripetal, and fiber insert according to the type of placement method (n = 10). Single-bond adhesive system was used as composite bonding according to the manufacturer's instructions. Specimens were restored in Groups 1, 2, and 3 with Filtek P60 and in Groups 4, 5, and 6 with Nulite F. After being stored 24 hours at 37°C, a 4 mm diameter steel sphere in a universal testing machine was applied on tooth buccal and lingual cusps at a cross-head speed of 5 mm/min until fracture occurred. Groups 3 and 6 showed higher fracture resistance than Groups 1, 2, 4, and 5. Among the placement techniques, the fiber insert method had a significant effect, but the type of composite was ineffective. The insertion technique in contrast to the type of material had a significant influence on the fracture resistance of premolar teeth

Thermal Changes in Root Surface of Primary Teeth During Root Canal Treatment With Diode Lasers: An In Vitro Study

Introduction: Increased temperature due to the application of laser during root canal disinfection may damage periodontium, alveolar bone, and permanent dental germ. The aim of this study was to evaluate the temperature increase of the external surface of primary roots due to the application of 810 nm and 980 nm diode lasers.Methods: A total of 58 extracted human primary teeth were prepared and randomly divided into two groups: (a) 810 nm diode laser and (b) 980 nm diode laser. Then, each group was divided into 4 subgroups based on the location of the temperature measurement, including subgroup 1: external root surface of primary anterior roots (A); subgroup 2: external root surface of posterior teeth at inter-root space (IS); subgroup 3: external root surface of posterior teeth at outer-root space (OS) and subgroup 4: external surface of furcation area of posterior teeth (F).Results: The mean temperature rise in group a (7.02±2.95ºC) was less than that of group b (10.62±4.59ºC) (P < 0.001). Also, a significant difference was found between the laser groups in terms of the mean temperature rise of the external root surface at IS, OS and F, with higher temperature increase occurring in all points in laser b. The comparison of irradiation points in each laser showed a higher mean temperature rise for IS than OS, but this difference was only significant in group b (P < 0.001).Conclusion: Within the studied parameters, 810 nm and 980 nm diode lasers should be used cautiously in primary root canals because of their temperature rise during their application

Histopathological and Biochemical Evaluation of Albendazole in the Treatment of Infected Mice with Hydatid Cyst

Abstract Introduction: Hydatidosis, caused by the larval stage of Echinococcus granulosus, is a prevalent parasitic disease affecting both humans and animals. Albendazole is currently the most effective drug for treating hydatid cysts. This research aimed to investigate the histopathological and biochemical effects of Albendazole on the liver, lung, and kidney of mice experimentally infected by hydatid cysts. Materials and methods: A total of 20 mice weighing approximately 220 g were used. The rats were randomly divided into the Albendazole group (100 mg/kg/day) and the control group (infected Rats without treatment). At the end of the experiment, tissue samples from the liver, lung, and kidney were collected for histopathological evaluation. Liver blood tests were used to assess liver functions or liver injury (alkaline phosphatase, alanine aminotransferase, and bilirubin). Results: After 30 days of daily treatment, the total numbers of cysts, size, and weight of the largest cyst were significantly lower in the Albendazole group, compared to the control group. The study addressed histopathological changes in the liver, kidneys, and lungs caused by hydatid cysts, such as tissue necrosis, hemorrhage, and local inflammation, indicating the potential for serious complications and significant damage to these organs. The group treated with Albendazole showed severe histopathological changes in the liver, kidneys, and lungs, compared to the control group. This suggests that Albendazole may trigger a more aggressive response in these organs to the cysts, leading to increased tissue damage. In addition, alkaline phosphatase, alanine aminotransferase, and bilirubin concentrations revealed a significant increase in the Albendazole group. Conclusion: While Albendazole is an effective drug for treating hydatidosis, it can also cause severe side effects on various organs in the body. Therefore, alternative treatment strategies need to be developed to minimize these adverse effects. https://jlar.rovedar.com/index.php/JLAR/article/view/

L-tryptophan production by Escherichia coli in the presence of Iranian cane molasses

The essential amino acid L-tryptophan (Trp) has importance as a pharmaceutical agent, especially in neuro-medicine. It is also added to feed products as a food fortifier. Furthermore, application of Trp is widespread in biotechnology. Trp is produced by a condensation reaction between indole and L-serine, which is catalyzed by bacterial tryptophanase activity. In this study, we have investigated Trp production using microbial system in the presence and absence of its precursors and Iranian cane molasses. The results showed that the optimum concentration of the molasses for maximum bacterial growth is 10 g/lit. Furthermore, in order to assay the amount of tryptophan produced, thin layer chromatography was used. The results showed that Iranian cane molasses contains considerable amounts of serine and indole, enough for Trp production (0.48 mM) in culture medium. But additional indole has inhibitory effects on Trp production. The data are compatible with previous reports on inhibitory effect of indole not only on cell growth but also on tryptophanase formation and function

Thermal aggregation of hen egg white lysozyme: effect of polyamines

Protein aggregation is a serious problem for both biotechnology and cell biology. Diseases such as prion misfolding, Alzheimer’s, and other amyloidosis are phenomena for which protein aggregation in our living cells is of considerable relevance. Human lysozyme has been shown to form amyloid fibrils in individuals suffering from nonneuropathic systemic amyloidosis, all of which have point mutations in the lysozyme gene. In this study, we investigated effect of small additives on the thermal aggregation of lysozyme. The main finding of this work is that multiple amine groups, spermine and spermidine, play pivotal roles in preventing the thermal aggregation of lysozyme. Our results showed that effect of spermine is more than spermidine

Controlled ∗-G-Frames and ∗-G-Multipliers in Hilbert Pro-C∗-Modules

A generalization of multiplier, controlled g-frames and g-Bessel sequences to ∗-g-frames and ∗-g-Bessel sequences in Hilbert pro-C∗-modules is presented. It is demonstrated that controlled ∗-g-frames are equivalent to ∗-g-frames in Hilbert pro-C∗-modules

Učinak kratkotrajne primjene hormona rasta i treninga snage na histopatološke promjene tkiva štitnjače i mutaciju gena BRAF (T1799A) u smeđeg štakora (Rattus norvegicus)

High levels of growth hormone accelerate mitosis rate but decrease the apoptosis process in its target organs. These events might cause the initiation of different cancer types. Thus, the main aims of this study were assessing the effects of short term growth hormone administration and resistance training on the histopathology and detection of the BRAF-V600E mutation in the thyroid tissue of male Rattus norvegicus brown rats. Thirty-two rats were randomly divided into four groups. After 8 weeks of the experiment (i.m), thyroid tissue and blood samples of saline (CS), resistance training+saline (RS), growth hormone (2 mg/kg) (GI) and resistance training+growth hormone (2 mg/kg) (RG) were taken to evaluate histopathology, the BRAF T1799A mutation of thyroid tissue, and circulating levels of IGF-1 and IGFBP-3. The protocol of training consisted of rats climbing a ladder while carrying weights (3 sets/5 reps). Microscopic evaluation of thyroid tissue did not show any histopathological changes, and there were no mutations in the studied region of the BRAF sequence. Serum IGF-1 concentration was significantly lower in the RS group than in other groups (P<0.05). However, serum IGFBP-3 concentration did not change significantly in the RS group. Moreover, serum IGF-1 and IGFBP-3 concentrations were significantly higher in the GI and RG groups than in the others (P<0.05). In conclusion, the decrement of serum IGF-1 concentration and IGF-1/IGFBP-3 ratio after resistance training might decrease the risk of cancer. Furthermore, short term growth hormone administration, with and without resistance training, might increase the risk of cancer through the high levels of serum IGF-1 concentration and IGF-1/ IGFBP-3 ratio in male rats.Visoke razine hormona rasta povećavaju brzinu mitoze, ali smanjuju proces apoptoze u ciljnim organima, što može uzrokovati nastanak različitih tipova raka. Stoga je glavni cilj ovoga istraživanja bio utvrditi učinak kratkotrajne primjene hormona rasta i treninga snage na histopatološke promjene tkiva štitnjače i nalaz mutacije gena BRAF-V600E u mužjaka smeđeg štakora Rattus norvegicus. Ukupno 32 štakora nasumično su podijeljena u četiri skupine koje su ovisno o primjenjenom tretmanu označene kao CS (fiziološka otopina), RS (trening snage + fiziološka otopina), GI (hormone rasta, 2 mg/kg) i RG (trening snage+hormone rasta, 2 mg/kg). Nakon osam tjedana pokusa (i.m.) uzeti su uzorci tkiva štitnjače i uzorci krvi kako bi se procijenila histopatološka svojstva, mutacija BRAF T1799A tkiva štitnjače te razine cirkulacijskog IGF-1 i IGFBP-3. Trening se sastojao od toga da se štakori penju ljestvama noseći težinu (3 seta vježbi, 5 ponavljanja). Mikroskopska procjena tkiva štitnjače nije pokazala histopatološke promjene i nije bilo mutacija u promatranoj regiji sekvencija gena BRAF. Koncentracija serumskog IGF-1 bila je znakovito manja u skupini RS nego u ostalim skupinama (P < 0,05), no u toj skupini nije bilo znakovite promjene u koncentraciji serumskog IGFBP-3. Štoviše, koncentracije serumskog IGF-1 i IGFBP-3 bile su znakovito veće u skupinama GI i RG nego u ostalim skupinama (P < 0,05). Stoga smo zaključili da bi smanjenje koncentracije serumskog IGF-1 i IGF-1/IGFBP-3 nakon treninga snage moglo smanjiti rizik od raka. Osim toga kratkotrajna primjena hormona rasta, s treningom snage kao i bez njega, mogla bi povećati rizik od raka povećavajući koncentracije serumskog IGF-1 i IGF-1/IGFBP-3 u mužjaka štakora