Promjene u sastavu proteina u mišiću stražnje lože (biceps femoris) iranske jednogrbe deve i njihov utjecaj na kakvoću mesa

In this study physicochemical and quality traits of biceps femoris and longissimus thoracis muscles of male and female Iranian one-humped camel were determined during 14 days of refrigeration storage. Analysis of variance of the results showed that only shear force and temperature were affected by the gender (p<0.05). Anatomical location of the muscle influenced the meat properties except for drip loss (p<0.05). Also, except for cooking loss, ageing influenced the physicochemical and quality properties of meat; during 14 days of storage, proteolysis resulted in an increase of L* and b* values, drip loss and myofibrillar fragmentation index, and the decrease of a* value, expressed juice, shear force and sarcomere length. Proteome changes (myofi brillar proteins) during storage were investigated. Gel analysis revealed that 19 protein spots were signifi cantly changed during 24, 72 and 168 h post-mortem. Fifteen spots were identified by MALDI-TOF/TOF mass spectrometer. Correlation analysis revealed significant correlations of actin, troponin T, capping protein, heat shock proteins (HSP) and desmin with physicochemical and quality properties of meat (p<0.05). Actin might be a potential protein marker for colour, tenderness and water-holding capacity, and HSP27 and desmin are good candidate markers for colour and tenderness, respectively.U ovom su radu određena fizikalno-kemijska svojstva i kakvoća mišića stražnje lože (biceps femoris) i uzdužnog grudnog mišića (longissimus thoracis) muške i ženske iranske jednogrbe deve tijekom 14 dana skladištenja u hladnjaku. Analiza je varijance pokazala da spol deve utječe jedino na silu presijecanja prsnih mišića i temperaturu mesa (p˂0.05). Anatomska lokacija mišića utjecala je na svojstva mesa, osim na iscjedni gubitak (p˂0.05). Starost je deve utjecala na fizikalno-kemijska svojstva (osim na gubitak prilikom kuhanja mesa) i kakvoću mesa. Tijekom 14 dana skladištenja zbog proteolize je došlo do povećanja vrijednosti parametara boje L* i b*, iscjednog gubitka i indeksa fragmentacije miofibrila, te do smanjenja vrijednosti parametra boje a*, mesnog soka, sile presijecanja prsnih mišića i duljine sarkomera. Pomoću proteomske metode ispitane su promjene u udjelu miofibrilarnih proteina tijekom skladištenja. Analiza gela otkrila je 19 bitno izmijenjenih elektroforetskih točaka proteina 24, 72 i 168 h post mortem. Pomoću MALDI-TOF-TOF masenog spektrometra identificirano je 15 elektroforetskih točaka. Analizom su otkrivene značajne korelacije između udjela aktina, troponina T, F-aktina, proteina toplinskog stresa te dezmina i kakvoće mesa (p˂0.05). Aktin bi se mogao upotrijebiti kao proteinski marker za boju, mekoću i sposobnost zadržavanja vode u mesu, protein toplinskog stresa HSP27 kao marker za boju, a dezmin za mekoću mesa

Physician-Patient Relationship: Ethnography of Multiple Patient Visits at Private Offices of Doctors

Introduction: The ways in which doctors and patients interact, have an incredible impact on the quality of treatment and improvement of the disease. Although most researches agree on the impact of these relationships on the quality of healthcare, not many researches and practical work have been done on this very important field. Moreover, recently the multiple patient visit phenomenon (seeing several patients simultaneously) at the doctor's offices, especially obstetricians, is becoming common, which is clearly far from the ideal doctors-patient relationship patterns. Method: In the current research the ethnographic qualitative approach has been used. For collecting data qualitative method of participatory observation and for data analysis the procedure of theoretical coding have been used. The target community is the women going to obstetricians' offices in Ahvaz, from which 28 cases of interactions between these patients and doctors have been chosen and observed through judgment sampling. Results: In the coding step the patients' verbal behavior in terms of 3 minor categories of asking questions, expressing concerns, comments and suggestions are subcategories for the major category of "patient participatory behavior". Furthermore, the doctor's verbal behavior is the main subcategory, and encouraging the patients to talk more, and respecting the patients' ideas and beliefs are the secondary subcategories of "doctor participatory behavior". Patients' and doctors' non-verbal behavior in the process of interactions has been recorded and put in the final analysis through filling out the observation protocol, and field notes. Conclusion: According to the analysis of the obtained data, only a few doctor-patient interactions at the studied doctors' offices were real evidence of participatory behavior. This behavior was observed far less in offices with multiple patient visits than in other offices. Understanding biological, social, and cultural aspects of the disease can affect the doctor’s communication with the patient. It can even help the doctor in understanding the patients’ issues and mental or physical problems, reaching an accurate diagnosis of the disease, and choosing appropriate and efficient treatment. Keywords: Physician-patient relationship, Doctor\'s office, Patients, Multiple patient visits, Participatory, Ethnograph

FeCl 3 .nano SiO 2 : An Efficient Heterogeneous Nano Catalyst for the Synthesis of 14-Aryl-14H-dibenzo[a,j]xanthenes and 1,8-Dioxo-octahydro-xanthenes under Solvent-free Conditions

ABSTRACT A novel, efficient and eco-friendly procedure for the synthesis of 14-aryl-14H-dibenzo[a,j]xanthenes and 1,8-dioxo-octahydroxanthenes is described through one-pot condensation of 2-naphthol and dimedone with aryl aldehydes in the presence of nano silica-supported ferric chloride under solvent-free conditions. The present approach offers several advantages such as short reaction times, high yields, easy purification, recovery and reusability of the catalyst

Association between Rotavirus Infection and Irritable Bowel Syndrome: A Case-control Study in Kerman - Iran

Background: Irritable bowel syndrome is a functional gastrointestinal disease of unknown etiology. Researchers have recently drawn attention to the possible role of viruses in the development of IBS and provided evidence in this regard. In this study, it was decided to investigate the possible role of rotavirus infection in the onset of IBS.Methods: Stool and serum samples were collected from 40 patients with IBS and 40 healthy individuals. To evaluate the previous exposure to rotavirus we checked the presence and concentration of anti-rotavirus IgG by ELISA. ELISA test was performed on the serum samples. Real-time PCR test was also used to measure the viral load in the stool. Finally, the data were analyzed by SPSS-22 software.Results: No significant relationship was found between anti-rotavirus IgG presence and Level in the serum of case and healthy individuals (p-value > 0.05) . Moreover, there was no significant difference between the viral genome load in the stool samples of the two groups (p-value > 0.05).Conclusion: According to the results, it seems unlikely that a link exists between rotavirus infection and the onset of irritable bowel syndrome, but the possible role of other gastrointestinal viruses, including coronavirus, remains

Correlation of Hematologic Indices with CT-pulmonary Arterial Obstruction Index in Patients with Acute Pulmonary Emboli

Introduction: Acute Pulmonary thromboembolism (PTE) is an imperative medical condition with a considerable global impact. Inflammation is deemed to take a notable part in the pathophysiology of this potentially fatal disorder. The aim of the current study was to predict acute PTE severity in helical pulmonary CT-angiography using easily accessible hematological complete blood count (CBC) indices.Methods: After exclusion of inflammatory conditions that may affect CBC parameters, a total of 69 consecutive patients with definite diagnosis of acute PTE according to pulmonary helical CT –angiography were recruited. Laboratory tests, including CBC parameters were performed on admission in the emergency unit, before initiation of any therapy. Neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR) and RDW to platelet ratio (RPR) were calculated in each case of acute PTE. Ultimately, CT pulmonary arterial obstruction index (PAOI) was assessed subsequent to pulmonary helical CT-angiography for each patient.Results: We found that NLR is positively correlated with acute PTE severity according to CT pulmonary arterial obstruction index (PAOI) (P &lt; 0.01, r = 0.56); however, PLR, RDW and RPR did not appear to show such correlations (P &gt; 0.05).Conclusions: NLR could be an easily calculated and capable index to predict severity of acute PTE in pulmonary CT-angiography. Consequently, NLR might be used in precise risk stratification when suspicious for acute PTE and in accurately triage of patients who would benefit greatly from urgent diagnostic and therapeutic interventions

Identifying the Challenges of Kerman University of Medical Sciences, Iran, in Achieving the Goals of Iran’s Health Scientific Road Map and Providing Strategies

Background & Objective: After preparing the health scientific road map, universities of medical sciences should play their role in this regard. This study aimed to identify the main challenges of Kerman University of Medical Sciences, Iran, in achieving the goals of the health scientific road map. Methods: This study was conducted as a qualitative study. The study population consisted of KMU`s top managers and experts. Purposeful and snowball sampling methods were used. 25 interviews were conducted in 2012. Using a semi-structured interview, the challenges of Kerman University of Medical Sciences in achieving the goals of health scientific road map were investigated. A framework method was applied for the data analysis. Results: At the end of this qualitative study, 10 basic concepts consisting of policy making and management, financing and facilitating processes, development and promotion of human resources, development and promotion of education, research, dissemination of knowledge, entrepreneurship, services provision, culture and norms, and facilitating communication, and 46 subgroups of challenges were identified. Conclusion: Given the importance of achieving the goals of health scientific road map and the importance of medical sciences universities in this regard, Kerman University of Medical sciences must develop a comprehensive plan to reduce the challenges. Keywords Health scientific road map Kerman University of Medical Sciences Iran Innovation syste

Evaluation Effect of Hydroalcoholic Extract of Eucalyptus globulus and Artemisia draconculus Compared with Acyclovir against Herpes Simplex Virus Type 1

Herpes simplex virus 1 and 2 (HSV-1 and HSV-2), also known as Human herpes virus 1 and 2 are two members of the herpes virus family, Herpesviridae, that infect humans. Both HSV-1 (which produces most cold sores) and HSV-2 (which produces most genital herpes) are ubiquitous and contagious. They can be spread when an infected person is producing and shedding the virus. The present study was carried out to determine the effect of alcoholic extract of two herbs Eucalyptus globulus and Artemisia draconculus on herpes virus compare with acyclovir in Iran during 2011. After preliminary survey, two herbs Eucalyptus globulus and Artemisia draconculus were selected as a drug for the treatment of herpes virus. HSV-1 was isolated from patients and identified by specific monoclonal antibodies. Vero cells (African green monkey kidney cells) were cultured with Dulbecco's Modified Eagles' Medium (DMEM) supplemented with 10% heat inactivated Fetal Bovine Serum (FBS), 100 IV/mL penicillin and l μg/mL streptomycin. Finally, the effect of these plants and Acyclovir compared with together on herpes virus. Results showed Artemisia draconculus could not reduce viral plaques significantly, however methanolic extracts of Eucalyptus globulus had a significant inhibitory effect against HSV-1 and concentration (200, 150, 50 μg/mL) has the best effect and (>200 μg/mL) Has lowest effect on HSV-1.The comparison of results exhibited that Eucalyptus globulus extract has more effects in different dilutions against HSV-1 in cell culture. (PDF) Evaluation effect of Hydroalcoholic Extract of Eucalyptus globulus and Artemisia draconculus compare with acyclovir against Herpes Simplex Virus Type 1. Available from: https://www.researchgate.net/publication/280769019_Evaluation_effect_of_Hydroalcoholic_Extract_of_Eucalyptus_globulus_and_Artemisia_draconculus_compare_with_acyclovir_against_Herpes_Simplex_Virus_Type_1 [accessed Sep 23 2018]