Feeding low or pharmacological concentrations of zinc oxide changes the hepatic proteome profiles in weaned piglets.

Pharmacological levels of zinc oxide can promote growth and health of weaning piglets, but the underlying molecular mechanisms are yet not fully understood. The aim of this study was to determine changes in the global hepatic protein expression in response to dietary zinc oxide in weaned piglets. Nine half-sib piglets were allocated to three dietary zinc treatment groups (50, 150, 2500 mg/kg dry matter). After 14 d, pigs were euthanized and liver samples taken. The increase in hepatic zinc concentration following dietary supplementation of zinc was accompanied by up-regulation of metallothionein mRNA and protein expression. Global hepatic protein profiles were obtained by two-dimensional difference gel electrophoresis following matrix-assisted laser desorption ionization/time-of-flight mass spectrometry. A total of 15 proteins were differentially (P<0.05) expressed between groups receiving control (150 mg/kg) or pharmacological levels of zinc (2500 mg/kg) with 7 down- (e.g. arginase1, thiosulfate sulfurtransferase, HSP70) and 8 up-regulated (e.g. apolipoprotein AI, transferrin, C1-tetrahydrofolate synthase) proteins. Additionally, three proteins were differentially expressed with low zinc supply (50 mg/kg Zn) in comparison to the control diet. The identified proteins were mainly associated with functions related to cellular stress, transport, metabolism, and signal transduction. The differential regulation was evaluated at the mRNA level and a subset of three proteins of different functional groups was selected for confirmation by western blotting. The results of this proteomic study suggest that zinc affects important liver functions such as blood protein secretion, protein metabolism, detoxification and redox homeostasis, thus supporting the hypothesis of intermediary effects of pharmacological levels of zinc oxide fed to pigs

Loess Records

Loess is aeolian sediment, dominated by silt-sized particles, that is identifiable in the field as a distinct sedimentary body. It covers a significant portion of the land surface of the Earth and as such constitutes one of the most important archives of long-term dust deposition. Large tracts of loess cover Europe, Asia, South America, and North America, and smaller loess bodies are found covering parts of Africa, the Middle East, New Zealand, and Australia. Loess thickness, particle size, and carbonate content decrease downwind from sources, trends that are powerful tools for reconstructing paleowinds. Many loess sections consist of relatively thick deposits of mostly unaltered sediment with intercalated paleosols. Paleosols represent periods of landscape stability when loess deposition ceased or at least slowed significantly. Studies from several continents show that loess in most regions was deposited during glacial periods and paleosols formed during interglacial and interstadial periods