Theoretical value of the recommended expanded European Standard Set of STR loci for the identification of human remains

We have undertaken a series of simulations to assess the effectiveness of commercially available sets of STR loci, including the loci recommended for inclusion in the expanded European Standard Set, for the purpose of human identification. A total of 9200 genotype simulations were performed using DNA · VIEW. The software was used to calculate likelihood ratios (LRs) for 23 groups of relatives, and to determine the probability of identification given scenarios that ranged between 10 and 250,000 victims. The additional loci included in the recommended expanded European Standard Set, when used in conjunction with the Identifiler® kit, significantly improved the typical LRs for tested scenarios and the likely success of providing correct identifications

Laser capture microdissection in forensic research: a review

In forensic sciences, short tandem repeat (STR) analysis has become the prime tool for DNA-based identification of the donor(s) of biological stains and/or traces. Many traces, however, contain cells and, hence, DNA, from more than a single individual, giving rise to mixed genotypes and the subsequent difficulties in interpreting the results. An even more challenging situation occurs when cells of a victim are much more abundant than the cells of the perpetrator. Therefore, the forensic community seeks to improve cell-separation methods in order to generate single-donor cell populations from a mixed trace in order to facilitate DNA typing and identification. Laser capture microdissection (LCM) offers a valuable tool for precise separation of specific cells. This review summarises all possible forensic applications of LCM, gives an overview of the staining and detection options, including automated detection and retrieval of cells of interest, and reviews the DNA extraction protocols compatible with LCM of cells from forensic samples

Epiphyseal union sequencing: aiding in the recognition and sorting of commingled remains

The presence of accessory osseous material within a seemingly single individual assemblage has the potential to result in misidentification of the remains. Detection of nonrelated material relies on the anthropologist being able to recognize incongruities among the elements. Inconsistencies in developmental status provide evidence to suggest that commingling may have occurred. Analyzing the sequence in which the various epiphyses unite can help to identify outlying elements that do not match the predicted developmental pattern of the remaining skeleton, thus indicating that the element may not belong to that individual. This paper considers the sequence in which 21 various epiphyses of the body unite to serve as a reference for identifying incongruent fusing patterns within a commingled assemblage. Two hundred and fifty-eight male individuals of Bosniak (Bosnian Muslim) descent between the ages of 14 and 30 years were included for analysis. Sequence order was determined for both ‘‘beginning’’ and ‘‘complete’’ union by comparing the fusing status of each epiphysis with each of the other 21 epiphyses. Considering both sequence patterns provides a wider spectrum of evidence from which to recognize incongruities than either sequence pattern could provide in isolation. Variations to the majority sequence pattern were also included to ensure that skeletons displaying less popular but acceptable sequence patterns would not be mistakenly considered as two individuals when using this research as a reference. Although substantial variation in the order in which epiphyses initiate and complete union was discovered within the sample, most epiphyseal relationships did not display any variable patterns. These ‘‘unvaried’’ relationships will be most useful in recognizing the presence of incongruent material if the pattern within an assemblage does not conform to the pattern documented in this study. Figures demonstrating the two sequence patterns are provided for easy application in the field