30 research outputs found
Glanders Diagnosis in an Asymptomatic Mare from Brazil: Insights from Serology, Microbiological Culture, Mass Spectrometry, and Genome Sequencing.
ABSTRACT - This manuscript elucidates the occurrence of glanders in an asymptomatic mare from Brazil presenting positive Burkholderia mallei antibody titers. The diagnosis was established through a multi-pronged approach encompassing microbiological culture, mass spectrometry, and genome sequencing. The outbreak occurred in 2019 in TatuĂ, SĂŁo Paulo, Brazil, and the infected mare, despite displaying no clinical symptoms, had multiple miliary lesions in the liver, as well as intense catarrhal discharge in the trachea. Samples were collected from various organs and subjected to bacterial isolation, molecular detection, and identification. The strain was identified as B. mallei using PCR and confirmed by MALDI-TOF mass spectrometry. Whole-genome sequencing revealed a genome size of 5.51 Mb with a GC content of 65.8%, 5871 genes (including 4 rRNA and 53 tRNA genes), and 5583 coding DNA sequences (CDSs). Additionally, 227 predicted pseudogenes were detected. In silico analysis of different genomic loci that allow for differentiation with Burkholderia pseudomallei confirmed the identity of the isolate as B. mallei, in addition to the characteristic genome size. The BAC 86/19 strain was identified as lineage 3, sublineage 2, which includes other strains from Brazil, India, and Iran. The genome sequencing of this strain provides valuable information that can be used to better understand the pathogen and its epidemiology, as well as to develop diagnostic tools for glanders
A arqueologia dos fermentados: a etĂlica histĂłria dos Tupi-Guarani
O consumo de bebidas fermentadas Ă© geralmente negligenciado pela literatura arqueolĂłgica, que trata a questĂŁo como tema de interesse secundĂĄrio (recreativo) na histĂłria das populaçÔes humanas. Entretanto, a literatura etnogrĂĄfica das sociedades indĂgenas das terras baixas sul-americanas indica exatamente o oposto: Ă© o alimento vegetal sĂłlido e nĂŁo alcoĂłlico que tende a possuir um papel secundĂĄrio na vida cotidiana e ritualĂstica de diversos coletivos. Os dados arqueolĂłgicos aprofundam temporalmente essa relação entre o ser humano e os fermentados. AlĂ©m disso, os vasos cerĂąmicos arqueolĂłgicos utilizados para o preparo e consumo desses fermentados sĂŁo fundamentais para a compreensĂŁo de processos e eventos histĂłricos que modelaram a dispersĂŁo de uma sĂ©rie de grupos pelo continente
Innovation in UK independent homecare services : a thematic narrative review
This paper reports the findings of a thematic narrative review of peer-reviewed articles exploring innovation in UK independent homecare services published between January 2009âAugust 2021. Our analysis of 15 papers reveals four broad innovation types: personalised funding, operational models, workforce development and assistive technology. We conclude that research focused on innovation in independent homecare offers important insights into the positive and negative outcomes of different types of innovation for providers, care workers and people receiving care. There are, however, also areas which are neglected and need further elaboration, including more robust evidence of outcomes and clearer articulation of innovation processes