Preliminary investigations of Agrobacterium-mediated transformation in indica rice MR219 embryogenic callus using gusA gene

Preliminary steps in the genetic transformation of indica rice MR219 was investigated in the plant- Agrobacterium tumefaciens interaction. Agrobacterium tumefaciens strain LBA 4404 carrying a binary vector pCAMBIA 1305.2 harboring the modified GUS gene driven by the CaMV 35S promoter was used. Various transformation parameters influences were optimized using embryogenic calli via β- glucuronidase (GUS) as a reporter marker. Various transformation parameters were optimized including bacterial concentration, age of embryogenic callus, pre-culture period, wounding technique, cocultivation period, immersion time and dry time before co-cultivation, acetosyringone (AS) concentration, pH of co-cultivation media and temperature of the co-cultivation period. The expression of the transient gusA gene in the plant genome was preliminary confirmed by histochemical GUS assay activity (as blue spots). The results from transient gusA gene expression of calli suggested that the Agrobacterium-mediated transfer system of T-DNA in indica rice MR219 was highly efficient. Therefore, the investigation of factors that influence T-DNA delivery is an important first step in the utilization of Agrobacterium in the transformation of indica rice MR219 calli.Key words: Indica rice MR219, Agrobacterium tumefaciens, GUS expression

Prevention of Radiocontrast-Induced Nephropathy after Coronary Angiography: N-Acetylcysteine plus Saline Hydration versus Saline Hydration

Purpose: To compare the incidence of radiocontrast-induced nephropathy (RCIN) in patients undergoing coronary angiography pretreated with N-acetylcysteine NAC plus saline hydration or saline hydration alone and to determine the association between various risk factors and RCIN.Methods: Patients were retrospectively evaluated over a one-year period. RCIN was defined as an acute rise in serum creatinine of at least 0.5 mg/dl or more than 25 % from baseline value. SPSS software, version 13 was used for data analysis.Results: Overall, 299 patients were studied. Thirteen patients (4.3%) developed RCIN. RCIN was 3.3 % in patients treated with hydration, and 8.3 % in patients treated with NAC plus hydration (p = 0.09). Inpatients with RCIN, the changes in creatinine and % change in creatinine after 24 h were significantly lower in the NAC plus hydration group (p = 0.039 and p = 0.042, respectively). RCIN was significantly associated with male gender (p = 0.017), history of renal failure (p = 0.006), ischemic heart disease (p =0.003), and diuretic treatment (p = 0.013).Conclusion: NAC plus saline hydration may not be more effective than saline hydration alone in decreasing RCIN after coronary angiography. Additional efforts to find an ideal preventive treatment are needed

Roles and Responsibilities Land Deed Official (PPAT) Of Tax Duty in the Implementation of Acquisition of Land and Buildings (BPHTB) Underpayment by the Taxpayers in Semarang

The purpose of this research are: 1) To identify and analyze the role of responsibility in the Land Deed Official BPHTB implementation of extant underpayment by the taxpayer in the city of Semarang. 2) To know and understand the procedures for implementing the tax collection of BPHTB the underpayment by the taxpayer in the city of Semarang. 3) To analyze the barriers and solutions in tax collection is less paid by the taxpayer in the city of Semarang.The methods of this research approach is empirical jurisdiction. Primary data was taken by the method of structured interviews, secondary data, and the data tertiary literature study and analysis by descriptive analytical method.Based on the results of data analysis concluded that: 1) The role and responsibilities of PPAT can vary in terms of solving problems regarding the lack of pay taxes BPHTB, first there are some of PPAT to omit some chapters in a deed of them, and some are not stated with a settlement notice or in the form of an appeal as officials land deed to the client or taxpayers.2) procession conducted by the tax authorities namely Apparatus State Civil circulate bills of underpayment of tax is intended to taxpayers who are still their underpayment of tax by nature force because the circulars issued by phisicus accordance with rule and regulations in force in accordance with Regulation Semarang City No. 2 of 2011 Chapter VII Article 15 paragraph 1 and 2. 3) Barriers often happens is that first were often there is an error in the calculation of the nominal value of the deposit amount of tax to be paid by the taxpayer to the local government, the second presence at the crux on the amount of tax rates provided to the taxpayer by the SPPTPBB both rural and urban, as well as the steps being taken are in accordance with agreed procedures, but it is still the amount of load that must be paid by the taxpayer. The solution to these problems that is better than the Land Deed Official should conduct socialization to the client then later if there is any underpayment of tax.Keywords: Empirical Juridical Review; Pay less Tax; Customs Tax Collection Procession Acquisition of Lan

Heavy metals burden of Keenjhar Lake, District Thatta, Sindh, Pakistan

Detection of heavy metals (HMs) content from Keenjhar Lake water was carried out monthly from January to December, 2003. Zinc, chromium, copper, iron, manganese, nickel and cadmium were analyzed by dual mode of analytical methods flame atomic absorption spectrometry and electrothermal atomic absorption spectrometry (FAAS and ETAAS) by multi element standard solution. The concentrations of zinc, chromium, copper, iron, manganese Nickel and cadmium were 1.4 to 104.3 μgL-1, 1.28 to 4.9 μgL-1, 0.6 to 7.3 μgL-1, 2 to 45.6 μgL-1, 0.2 to 6.7 μgL-1, 3.54 to 39.5 μgL-1 and 1.0 to 4.3 μgL-1, respectively. Zn2+, Cr+, Cu2+, Fe2+ and Mn2+ concentrations were recorded lower than those of permissible limits, whereas Ni+ and Cd+ were higher than those of permissible limits recommended by WHO (2004).Key words: Heavy metals, Keenjhar Lake, toxicity

Abnormal Ophiolite (Olivine/Pyroxene Rich) Sandstone NE Iraq: An Approach to the Origin and Tectonosedimentary Evolution of Zagros Foreland Basin

Unusual Paleocene ophiolite sandstone rich in olivine/pyroxene identified in Zagros Thrust Belt (NZTB) in NE Iraq. NZTB is regionally extended from Iran to Alpen Belt. Kolosh sandstones are controlled by progressive thrusting during late Cretaceous-Paleocene. Zagros thrust sheets composed of ophiolites, oceanic crust, basaltic flows, and ash sequences. Kolosh sandstones reveal high percentages of fresh olivine-pyroxene grains accompanied by igneous intrusive and volcanic ultrabasic-basic fragments, which are reported for the first time in NE Iraq and along ZTB. Olivine, pyroxene, ultrabasic igneous altered, serpentine and chlorite fragments, heavy minerals (includes chrome spinal), anorthite, and labradorite all together composed about 70% of the mineralogical composition. Sanidine, anorthoclase, quartz and cristobalite, argillaceous, carbonate and chert fragments all together composed (12.25%), supported by argillaceous matrix (16.53%), which are derived from mantle and oceanic crust/ophiolite sequences from NE Iraq, emplaced during late Cretaceous with arc volcanism, which subjected to rapid submarine erosion and deposition. Intense wave action accelerated the erosion of beach rocks, and concentrate the heavy minerals insitue that slumped to deeper margins. Identified lithofacies types, grouped in four associations, slope/submarine channel, inner, outer fan, and hemipelagic/pelagic, respectively, represented progressive upward transgression from slope to basin plain systems controlled by progressive thrusting

ANALISIS KONTRIBUSI PENDAPATAN BAGI HASIL PAJAK KENDARAAN BERMOTOR DAN BEA BALIK NAMA KENDARAAN UNTUK DANA PEMBANGUNAN DAERAH KOTA MALANG PERIODE 2010-2014.

This study is motivated by the revenue receipt of (Motor Vehicle Tax) and (Customs of Motor Vehicle) Profit Sharing of Malang City that is increased and decreased over the period 2010-2014 by seeing its contribution to regional development fund. The method used is qualitative method descriptive research,using observation and documentation as the tools of data collection. The conclusion obtained from this study is that the realization of revenue of the Motor Vehicle Tax and Customs of Motor Vehicle Profit Sharing has reached the defined target despite there is unreachable target in certain year. The growth rate of revenue of Motor Vehicle Tax Profit Sharing has increased and decreased, but revenue growth of Customs of Motor Vehicle Profit Sharing experiences a steady increase, the level of local financial dependence of Malang City is rated so hight on revenue of Motor Vehicle Tax and Customs of Motor Vehicel) Profit Sharing. Contribution of revenue of Motor Vehicle Tax and Customs of Motor Vehicle Profit Sharing is so hight for regional development fund of Malang City. Keyword : the revenue of Motor Vehicle Tax and Customs of Motor Vehicle, regional development fund, Contribution   ABSTRAK Penelitian ini dilatar belakangi oleh penerimaan Bagi Hasil sektor Pajak Kendaraan Bermotor serta Bea Balik Nama Kendaraan Bermotor Kota Malang yang mengalami peningkatan dan penurunan selama periode 2010-2014 dengan melihat kontribusinya untuk dana Pembangunan Daerah. Metode penelitian yang dipilih yaitu metode kualitatif deskriptif, sebagai alat pengumpulan datanya menggunakan observasi serta dokumentasi. Kesimpulan yang diperoleh dari penelitian ini bahwa realisasi pendapatan Bagi Hasil Pajak Kendaraan Bermotor dan Bea Balik Nama Kendaraan Bermotor telah mencapai target meskipun ada ketidaktercapaian di tahun tertentu. Pertumbuhannya mengalami peningkatan dan penurunan, akan tetapi pendapatannya mengalami peningkatan yang stabil, tingkat ketergantungan keuangan daerah Kota Malang dinilai sangat tinggi. Kontribusi pendapatan Bagi Hasil PKB dan BBNKB dinilai sangat tinggi untuk dana pembangunan daerah Kota Malang. Kata Kunci : Bagi Hasil Pajak, Pembangunan Daerah, Kontribus

Modeling of Groundwater Potential Using Cloud Computing Platform: A Case Study from Nineveh Plain, Northern Iraq

Knowledge of the groundwater potential, especially in an arid region, can play a major role in planning the sustainable management of groundwater resources. In this study, nine machine learning (ML) algorithms—namely, Artificial Neural Network (ANN), Decision Jungle (DJ), Averaged Perceptron (AP), Bayes Point Machine (BPM), Decision Forest (DF), Locally-Deep Support Vector Machine (LD-SVM), Boosted Decision Tree (BDT), Logistic Regression (LG), and Support Vector Machine (SVM)—were run on the Microsoft Azure cloud computing platform to model the groundwater potential. We investigated the relationship between 512 operating boreholes with a specified specific capacity and 14 groundwater-influencing occurrence factors. The unconfined aquifer in the Nineveh plain, Mosul Governorate, northern Iraq, was used as a case study. The groundwater-influencing factors used included elevation, slope, curvature, topographic wetness index, stream power index, soil, land use/land cover (LULC), geology, drainage density, aquifer saturated thickness, aquifer hydraulic conductivity, aquifer specific yield, depth to groundwater, distance to faults, and fault density. Analysis of the contribution of these factors in groundwater potential using information gain ratio indicated that aquifer saturated thickness, rainfall, hydraulic conductivity, depth to groundwater, specific yield, and elevation were the most important factors (average merit \u3e 0.1), followed by geology, fault density, drainage density, soil, LULC, and distance to faults (average merit \u3c 0.1). The average merits for the remaining factors were zero, and thus, these factors were removed from the analysis. When the selected ML classifiers were used to estimate groundwater potential in the Azure cloud computing environment, the DJ and BDT models performed the best in terms of all statistical error measures used (accuracy, precision, recall, F-score, and area under the receiver operating characteristics curve), followed by DF and LD-SVM. The probability of groundwater potential from these algorithms was mapped and visualized into five groundwater potential zones: very low, low, moderate, high, and very high, which correspond to the northern (very low to low), southern (moderate), and middle (high to very high) portions of the study area. Using a cloud computing service provides an improved platform for quickly and cheaply running and testing different algorithms for predicting groundwater potential

The Degree of Effectiveness of the Educational Decision-Making Process among the Principals of Zarqa Kasbah Schools from the Teachers\u27 Point of View درجة فاعلية عملية اتخاذ القرارات التربوية لدى مديري مدارس تربية قصبة الزرقاء من وجهة نظر المعلمين

Abstract: The study aimed at identifying the degree of effectiveness of the educational decision-making process of the principals of Zarqa Kasbah from the teachers\u27 point of view. It, also, studied the effect of variables on the response of the sample of the study. The study used the descriptive survey methodology. The tool of the study consisted of (14) items, and the validity and reliability of the tool was confirmed. The study was applied on a sample of (375) male and female teachers, who were chosen by a simple random method. The study proved the following results: the degree of effectiveness of educational decision-making process of the principals of Zarqa Kasbah education school from the teachers’ point of view, was medium. The results indicated that there were statistically significant differences at the level (0.05\u3ea) in the mean. Responses of the study sample subjects about the effectiveness of the educational decision-making process of the school principals of Zarqa Kasbah education schools, from the teachers \u27viewpoint. These differences were due to the variables of gender and academic qualification. Moreover, it indicated that there were no statistically significant differences due to the variables of years of experience and specialization. In light of the proved results, the study recommended a set of recommendations, the most important of which are: increasing the participation of teachers in the school decision­ making process, in the way that reflected in the raising the effectiveness of educational decisions. ملخص: هدفت الدراسة التعرف إلى درجة فاعلية عملية اتخاذ القرارات التربوية لدى مديري مدارس تربية قصبة الزرقاء من وجهة نظر المعلمين ودراسة أثر المتغيرات في استجابة افراد عينة الدراسة، واستخدمت الدراسة المنهج الوصفي المسحي وتم تطوير أداة دراسة تكونت من (14) فقرة وتم التأكد من صدق الأداة وثباتها. وطبقت الدراسة على عينة بلغت (375) معلماً ومعلمة وتم اختيارهم بالطريقة العشوائية البسيطة وتوصلت الدراسة إلى النتائج الآتية: إن درجة فاعلية عملية اتخاذ القرارات التربوية لدى مديري مدارس تربية قصبة الزرقاء من وجهة نظر المعلمين جاءت متوسطة، بينما دلت النتائج إلى وجود فروق ذات دلالة احصائية عند مستوى (0.05≥α) في متوسط استجابات أفراد عينة الدراسة عن فاعلية عملية اتخاذ القرارات التربوية لدى مديري مدارس تربية قصبة الزرقاء من وجهة نظر المعلمين تعزى لمتغير الجنس والمؤهل العلمي بينما دلت إلى عدم وجود فروق ذات دلالة احصائية تعزى لمتغيري سنوات الخبرة والتخصص. وفي ضوء نتائج الدراسة أوصت الدراسة بمجموعة من التوصيات من أهميها: زيادة مشاركة المعلمين في عملية اتخاذ القرارات المدرسية بالشكل الذي ينعكس على زيادة فاعلية القرارات التربوية

Antibiosis in Ascia monuste orseis Godart (Lepidoptera: Pieridae) caused by kale genotypes

Ascia monuste orseis (Lepidoptera: Pieridae) is one of the main insect pests of kale. The study was done to  identify kale varieties resistant to A. monuste orseis by the antibiosis resistance mechanism. Kale genotypes (26) were evaluated in experiments performed at the Laboratory of Agricultural Entomology of Goiano Federal Institute - Campus Urutaí. A completely randomized experimental design with 50 replicates was used. The biological parameters evaluated were (a) larval stage: development time, instars, viability and larval weight 15 days after hatching; (b) pupal stage: development time, weight of 24-h-old pupae, viability; (c) larvae-adult stage: development time and viability. The genotypes Gigante I-915 and Pires 1 de Campinas have antibiosis resistance. Gigante I-915 caused high larval mortality and Pires 1 de Campinas resulted in low larval and pupal viability of A. monuste orseis.Key words: Brassica oleracea L. var. acephala, Brassicaceae, Great Southern White, host plant resistance, integrated pest management (IPM)